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The map label for this gene is hisF [H]

Identifier: 148241678

GI number: 148241678

Start: 513652

End: 514488

Strand: Direct

Name: hisF [H]

Synonym: SynRCC307_0579

Alternate gene names: 148241678

Gene position: 513652-514488 (Clockwise)

Preceding gene: 148241673

Following gene: 148241679

Centisome position: 23.09

GC content: 64.87

Gene sequence:

>837_bases
GTGCTGACTGGGTTTCGCGGCAACCTTACCAGCGCCATCTCGTTCCTTTGTGCTGCTGGTGCTGCTGTGGTTGCTAAACG
GATCATTCCCTGCCTCGACATGGCTGCCGGCCGCGTGGTGAAGGGGGTGAATTTCGTGGGCCTGCGCGATGCCGGCGATC
CCGTGGAGCTGGCCTGCCGCTACGACGCTGCGGGCGCCGATGAATTGGTGTTTCTCGATATCGCCGCCACCCATCAAGGC
CGCGAAACCCTGGTGGAGCTGGTGGAGCGCACCGCCTCGCAGCTGTTCATTCCGTTCACCGTTGGTGGTGGAATTCGCTC
GCTTGAGGGCATCACGGCGCTGTTGCGGGCTGGTGCCGACAAGGTCAGCCTGAATTCCTCAGCGGTGAAGGATCCCGAGC
TGATCCGCGCCGGGGCCCGCCAGTTCGGAGCCCAGTGCATCGTGGTGGCGATCGATGCTCGCCGCGGCGCCGGTGGTGGC
TGGGATGTCTATGTCAAAGGAGGCCGCGAGAACACCGGCCTCGATGCCATCGCTTGGGCCCAGCAAGTGGTGGAGTGCGG
CGCGGGGGAATTGCTGCTCACGTCCATGGACGGTGATGGCACCCAAAAGGGTTACGACCTTGAGCTCACCGCAGCCATTG
CCGATGCGGTGAGCGTGCCAGTGATTGCCTCTGGTGGGGCGGGCTCTCTGGATCACATCGCTGAGGTGCTGGAGTCCACC
GGGGCCGCCGCTGCACTGCTGGCGTCGCTCCTGCATGACGGCGTGCTCACGGTGGAGCAGATCAAGGCTGATTTGCAGCG
CCGCGGTGTGCCTGTGCGGCCGCTGGTGGACGCTTGA

Upstream 100 bases:

>100_bases
TTGCCGAGCAGCAGCTTCACCAGATCTGCCGGGATGCGATCACGCACGCGGGTGATTTTCACCTTGGATCCCACGTTGAT
GGCAGGCTGGGACATCGACG

Downstream 100 bases:

>100_bases
GTTCGTTAAGTTGTCTGTGACATTTCTTACTAAATGGGGTTCAACGGATTGGATCTGTTACCCGGATTGATTGGTTTATT
GCTCGTGGCCGTGGTGATCA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF1; ImGP synthase subunit hisF1; IGPS subunit hisF1 [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MLTGFRGNLTSAISFLCAAGAAVVAKRIIPCLDMAAGRVVKGVNFVGLRDAGDPVELACRYDAAGADELVFLDIAATHQG
RETLVELVERTASQLFIPFTVGGGIRSLEGITALLRAGADKVSLNSSAVKDPELIRAGARQFGAQCIVVAIDARRGAGGG
WDVYVKGGRENTGLDAIAWAQQVVECGAGELLLTSMDGDGTQKGYDLELTAAIADAVSVPVIASGGAGSLDHIAEVLEST
GAAAALLASLLHDGVLTVEQIKADLQRRGVPVRPLVDA

Sequences:

>Translated_278_residues
MLTGFRGNLTSAISFLCAAGAAVVAKRIIPCLDMAAGRVVKGVNFVGLRDAGDPVELACRYDAAGADELVFLDIAATHQG
RETLVELVERTASQLFIPFTVGGGIRSLEGITALLRAGADKVSLNSSAVKDPELIRAGARQFGAQCIVVAIDARRGAGGG
WDVYVKGGRENTGLDAIAWAQQVVECGAGELLLTSMDGDGTQKGYDLELTAAIADAVSVPVIASGGAGSLDHIAEVLEST
GAAAALLASLLHDGVLTVEQIKADLQRRGVPVRPLVDA
>Mature_278_residues
MLTGFRGNLTSAISFLCAAGAAVVAKRIIPCLDMAAGRVVKGVNFVGLRDAGDPVELACRYDAAGADELVFLDIAATHQG
RETLVELVERTASQLFIPFTVGGGIRSLEGITALLRAGADKVSLNSSAVKDPELIRAGARQFGAQCIVVAIDARRGAGGG
WDVYVKGGRENTGLDAIAWAQQVVECGAGELLLTSMDGDGTQKGYDLELTAAIADAVSVPVIASGGAGSLDHIAEVLEST
GAAAALLASLLHDGVLTVEQIKADLQRRGVPVRPLVDA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=44.921875, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI87082028, Length=237, Percent_Identity=31.6455696202532, Blast_Score=102, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=33.8658146964856, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28562; Mature: 28562

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTGFRGNLTSAISFLCAAGAAVVAKRIIPCLDMAAGRVVKGVNFVGLRDAGDPVELACR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEE
YDAAGADELVFLDIAATHQGRETLVELVERTASQLFIPFTVGGGIRSLEGITALLRAGAD
ECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCC
KVSLNSSAVKDPELIRAGARQFGAQCIVVAIDARRGAGGGWDVYVKGGRENTGLDAIAWA
CEECCCCCCCCHHHHHHHHHHHCCEEEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHH
QQVVECGAGELLLTSMDGDGTQKGYDLELTAAIADAVSVPVIASGGAGSLDHIAEVLEST
HHHHHCCCCCEEEEECCCCCCCCCCCEEEHHHHHHHHCCCEEECCCCCCHHHHHHHHHCC
GAAAALLASLLHDGVLTVEQIKADLQRRGVPVRPLVDA
CHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLTGFRGNLTSAISFLCAAGAAVVAKRIIPCLDMAAGRVVKGVNFVGLRDAGDPVELACR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEE
YDAAGADELVFLDIAATHQGRETLVELVERTASQLFIPFTVGGGIRSLEGITALLRAGAD
ECCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCC
KVSLNSSAVKDPELIRAGARQFGAQCIVVAIDARRGAGGGWDVYVKGGRENTGLDAIAWA
CEECCCCCCCCHHHHHHHHHHHCCEEEEEEEECCCCCCCCEEEEEECCCCCCCCHHHHHH
QQVVECGAGELLLTSMDGDGTQKGYDLELTAAIADAVSVPVIASGGAGSLDHIAEVLEST
HHHHHCCCCCEEEEECCCCCCCCCCCEEEHHHHHHHHCCCEEECCCCCCHHHHHHHHHCC
GAAAALLASLLHDGVLTVEQIKADLQRRGVPVRPLVDA
CHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]