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The map label for this gene is tbpA [H]

Identifier: 147675627

GI number: 147675627

Start: 2274514

End: 2275506

Strand: Reverse

Name: tbpA [H]

Synonym: VC0395_A2119

Alternate gene names: 147675627

Gene position: 2275506-2274514 (Counterclockwise)

Preceding gene: 147673199

Following gene: 147675055

Centisome position: 75.25

GC content: 50.45

Gene sequence:

>993_bases
TTGAAATTTCAACTGACTGCGGTTGCGTTAGCAACCCTATGTGTTGCCTCAGCAGCGTCTGCTGCACCAACCCTCACCGT
TTACACTTACGACTCTTTTGCCGCCGAATGGGGACCTGCACCTGCGATTGAAAAAGCGTTTGAAGCACAATGTGGCTGCG
ATCTGCAATTTGTCGCTTTGGATGATGGCGTGTCGATTCTTAACCGTCTGCGTTTGGAAGGCAGCAACAGCAAAGCCGAT
GTGGTGCTGGGGCTCGATAACAACCTTATCGAAGAAGCCAAGCAAACCGGATTATTGGCGACCCACAGTGTGGATACCAG
CAAGGTGACTCTGCCCAATGGCTGGAGTGACAACACATTTGTGCCTTACGACTATGGCTATTTCGCTTTCGTCTATAACA
AAGAGAAGCTGGCTAACCCGCCAACCAGCTTGAAAGAGCTGGTGGAAAAACGTGATGATCTGAAAGTGATCTACCAAGAC
CCGCGCACTTCAACGCCGGGACAAGGTTTACTGCTGTGGATGAAATCTGTCTATGGCGACCAAGCGCCACAAGCGTGGAA
GCAGTTGGCGAGCAAAACGGTCACTGTGACCAAAGGTTGGTCAGAAGCTTACTCTATGTTTCTCGGTGGAGAGGCGGATC
TGGTGCTCTCTTACACCACATCGCCGGCCTATCATTTGATTGCAGAAAATGATGCACGTTTTGCCACAGCGAATTTCAGC
GAGGGGCATTACCTGCAAGTGGAAGTGGCCGCCAAAGTCAAAAGCACTAAAAATGGCGAGCTGGCGGACAAGTTCCTGCA
ATTTATCCTGAGTAATGAGTTCCAAAGCGTGATCCCAACGGGTAACTGGATGTATCCAGTGACGGCGGTTGAACTGCCAA
AAGGGTTTGATACTTTGGCGGTACCGACACAATCTCTTTCTTTCAGCGCACAGGAAGTCGCGCAAATGCGTAAGCCATGG
ATTCGTGAGTGGCAGCAAGCGCTGAGCTTTTAA

Upstream 100 bases:

>100_bases
GATAATGCTGGCGTAGGAATTGAGCTAGGTTTGCAGAATGGAATAATGGACGATTCTCTGCCTCCCTCAAGCCTGTGCCG
CTCATTAAGGAGAGCGAGCC

Downstream 100 bases:

>100_bases
GGTTAAGCGTTGAATCGAACTCCTCAATTAGGCTTTTGGGTCGCGGCTGGTGTCGCGACCTTTGTCCTCGCGGCATTAGC
GGCGCTGCTCTGGCAAGCGC

Product: thiamine transporter substrate binding subunit

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKFQLTAVALATLCVASAASAAPTLTVYTYDSFAAEWGPAPAIEKAFEAQCGCDLQFVALDDGVSILNRLRLEGSNSKAD
VVLGLDNNLIEEAKQTGLLATHSVDTSKVTLPNGWSDNTFVPYDYGYFAFVYNKEKLANPPTSLKELVEKRDDLKVIYQD
PRTSTPGQGLLLWMKSVYGDQAPQAWKQLASKTVTVTKGWSEAYSMFLGGEADLVLSYTTSPAYHLIAENDARFATANFS
EGHYLQVEVAAKVKSTKNGELADKFLQFILSNEFQSVIPTGNWMYPVTAVELPKGFDTLAVPTQSLSFSAQEVAQMRKPW
IREWQQALSF

Sequences:

>Translated_330_residues
MKFQLTAVALATLCVASAASAAPTLTVYTYDSFAAEWGPAPAIEKAFEAQCGCDLQFVALDDGVSILNRLRLEGSNSKAD
VVLGLDNNLIEEAKQTGLLATHSVDTSKVTLPNGWSDNTFVPYDYGYFAFVYNKEKLANPPTSLKELVEKRDDLKVIYQD
PRTSTPGQGLLLWMKSVYGDQAPQAWKQLASKTVTVTKGWSEAYSMFLGGEADLVLSYTTSPAYHLIAENDARFATANFS
EGHYLQVEVAAKVKSTKNGELADKFLQFILSNEFQSVIPTGNWMYPVTAVELPKGFDTLAVPTQSLSFSAQEVAQMRKPW
IREWQQALSF
>Mature_330_residues
MKFQLTAVALATLCVASAASAAPTLTVYTYDSFAAEWGPAPAIEKAFEAQCGCDLQFVALDDGVSILNRLRLEGSNSKAD
VVLGLDNNLIEEAKQTGLLATHSVDTSKVTLPNGWSDNTFVPYDYGYFAFVYNKEKLANPPTSLKELVEKRDDLKVIYQD
PRTSTPGQGLLLWMKSVYGDQAPQAWKQLASKTVTVTKGWSEAYSMFLGGEADLVLSYTTSPAYHLIAENDARFATANFS
EGHYLQVEVAAKVKSTKNGELADKFLQFILSNEFQSVIPTGNWMYPVTAVELPKGFDTLAVPTQSLSFSAQEVAQMRKPW
IREWQQALSF

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import [H]

COG id: COG4143

COG function: function code H; ABC-type thiamine transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 1 family [H]

Homologues:

Organism=Escherichia coli, GI1786255, Length=309, Percent_Identity=66.9902912621359, Blast_Score=446, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR006061
- InterPro:   IPR005948
- InterPro:   IPR005967 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36318; Mature: 36318

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFQLTAVALATLCVASAASAAPTLTVYTYDSFAAEWGPAPAIEKAFEAQCGCDLQFVAL
CCEEHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHCCCCCHHHHHHHHHHCCCCEEEEEE
DDGVSILNRLRLEGSNSKADVVLGLDNNLIEEAKQTGLLATHSVDTSKVTLPNGWSDNTF
CCCHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCEEEEECCCCCEEECCCCCCCCCE
VPYDYGYFAFVYNKEKLANPPTSLKELVEKRDDLKVIYQDPRTSTPGQGLLLWMKSVYGD
EEECCCEEEEEECHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCC
QAPQAWKQLASKTVTVTKGWSEAYSMFLGGEADLVLSYTTSPAYHLIAENDARFATANFS
CCHHHHHHHHHCEEEEECCHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCEEEEECCC
EGHYLQVEVAAKVKSTKNGELADKFLQFILSNEFQSVIPTGNWMYPVTAVELPKGFDTLA
CCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHCCCCCEEEEEEEEECCCCCCEEE
VPTQSLSFSAQEVAQMRKPWIREWQQALSF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFQLTAVALATLCVASAASAAPTLTVYTYDSFAAEWGPAPAIEKAFEAQCGCDLQFVAL
CCEEHHHHHHHHHHHHHHCCCCCEEEEEEECCHHHCCCCCHHHHHHHHHHCCCCEEEEEE
DDGVSILNRLRLEGSNSKADVVLGLDNNLIEEAKQTGLLATHSVDTSKVTLPNGWSDNTF
CCCHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHCCEEEEECCCCCEEECCCCCCCCCE
VPYDYGYFAFVYNKEKLANPPTSLKELVEKRDDLKVIYQDPRTSTPGQGLLLWMKSVYGD
EEECCCEEEEEECHHHHCCCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHCCC
QAPQAWKQLASKTVTVTKGWSEAYSMFLGGEADLVLSYTTSPAYHLIAENDARFATANFS
CCHHHHHHHHHCEEEEECCHHHHHHHHCCCCCEEEEEEECCCEEEEEECCCCEEEEECCC
EGHYLQVEVAAKVKSTKNGELADKFLQFILSNEFQSVIPTGNWMYPVTAVELPKGFDTLA
CCCEEEEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHCCCCCEEEEEEEEECCCCCCEEE
VPTQSLSFSAQEVAQMRKPWIREWQQALSF
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]