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The map label for this gene is thiP [H]

Identifier: 147675055

GI number: 147675055

Start: 2272912

End: 2274504

Strand: Reverse

Name: thiP [H]

Synonym: VC0395_A2118

Alternate gene names: 147675055

Gene position: 2274504-2272912 (Counterclockwise)

Preceding gene: 147675627

Following gene: 147674110

Centisome position: 75.21

GC content: 52.54

Gene sequence:

>1593_bases
TTGAATCGAACTCCTCAATTAGGCTTTTGGGTCGCGGCTGGTGTCGCGACCTTTGTCCTCGCGGCATTAGCGGCGCTGCT
CTGGCAAGCGCCACCGCTCAATCTGAATCTGATCTGGCAAGATGCCTATTATCGGCATGTTACCCAGTTCAGCTTTTATC
AAGCCTTCTGGTCCACCGTGCTCAGTGTGGGGTTGGCAATTCCGGTTGCTCATGCCCTCAGTCAGCGCCGTTTCCCCGGT
CGTGATTTACTACTGAAACTGTTCGCGATGACGCTGGTTTTGCCCGTACTGGTGGCGATTTTTGGTTTAATCGCGATTTA
CGGTAATAGTGGTTTTTTGGCCAAAACGCTGCACAGTGTCGGGCTCTCGCTGCCGTTTTCGATTTATGGTTTAAACGGTA
TTTTACTTGCCCATGTACTGTTTAATTTGCCTTATGCCAGCCGTTTGCTGCTGCAAAGTTTAGAAGCGATCCCCAGTGAG
CAGCACAAGCTGTGCGCCCATCTCGGCATGAGCCGTTGGCAAAAGTTTAAGTGGGTCGAGTGGCCACGCTTGCGTCAGCA
ACTGCCGCACGTTGCAGGCTTGGTGTTTATGCTTTGCTTTACTAGCTTTGCAACCGTGATGGCGCTCGGCGGCGGGCCTA
AAGCAACCACCATTGAGCTCGCGATCTACCAAGCGATCAAATTCGATTTCGATCTGCAAATTGGCGCGTTATTGGCGATT
TGGCAGATGTTGCTATGTACCTTACTGACTTTTACCCTGCAGCGTTTTTCGCATTCACGCCCCATGAACAGTTCCGGGAG
TAAGGTGACTGAGCGTTGGGTACGTGATTCATGGCGCGCCAAAGTTTGGGATACGAGCTGGATTGTCGCCGCATGTGTCT
TGGTATTGCCACCATTAGTGATGGTGGTGCTGGCAGGGCTCAATTCAAAGCTTGGCAAGGTACTGACGAGCGGCGATTTT
TGGTCGGCAGTCAGCCACTCTTTGCAAGTGGCGAGCTTAGCCAGTAGCTTGGCTCTGGCCAGTGGAATCGCGATGTTACT
CACCAGCCGAGCCTTGCGGTTGAAAGCGGCTCATCGCCGCGCCAATGGCGTGGAGTGGATCGCGACGTTAATTCTGGTCA
CGCCGGGATTAGTGGTGAGTACCGGACTCTTTTTGTTGCTGCGTAATATCACCGATGTGTTTAGTTTAGCTTACTGGATT
GTGGTCGCAGTCAACGCGCTAATGGCGCTGCCTTACGTCGTAAAAACCTTGAGCCAACCTATGTGGCACTTGGCTCAGCA
ATATGAATTGCTGTGCGCCAGTTTAGGCATGTCTGGTTGGCGTCGATTTCGTTTAGTGGAGTGGCGCGCGCTGCGTAAGC
CTCTCTCCCATGCGTTTGCGATCGGTTTTCTGCTCTCGATGGGGGATTTAAGCGCAATTGCCCTGTTTGGTAGCCAAGAT
TTTCGCACACTGCCGCTCTATCTTTATCAACTGCTCGGCAGCTATCAGATGGATACCGCGGCGGTCGTTGCGTTGGCCAT
GCTGATCATGAGTGTGGGTTGGTTTTATGTGATTGAGCGGCTGTTTGCCCAGCGTCCGCTTACCAAACGCTAA

Upstream 100 bases:

>100_bases
ACCGACACAATCTCTTTCTTTCAGCGCACAGGAAGTCGCGCAAATGCGTAAGCCATGGATTCGTGAGTGGCAGCAAGCGC
TGAGCTTTTAAGGTTAAGCG

Downstream 100 bases:

>100_bases
TAAGGAAAAGCATGTTAGCTCTAGATAAAGTGCGTTACGAATATGAGCACGAATGGTTCGAGTTTGATCTGAACGTGGCT
GATGGCGACATTGTGGCGCT

Product: thiamine transporter membrane protein

Products: ADP; phosphate; thiamine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MNRTPQLGFWVAAGVATFVLAALAALLWQAPPLNLNLIWQDAYYRHVTQFSFYQAFWSTVLSVGLAIPVAHALSQRRFPG
RDLLLKLFAMTLVLPVLVAIFGLIAIYGNSGFLAKTLHSVGLSLPFSIYGLNGILLAHVLFNLPYASRLLLQSLEAIPSE
QHKLCAHLGMSRWQKFKWVEWPRLRQQLPHVAGLVFMLCFTSFATVMALGGGPKATTIELAIYQAIKFDFDLQIGALLAI
WQMLLCTLLTFTLQRFSHSRPMNSSGSKVTERWVRDSWRAKVWDTSWIVAACVLVLPPLVMVVLAGLNSKLGKVLTSGDF
WSAVSHSLQVASLASSLALASGIAMLLTSRALRLKAAHRRANGVEWIATLILVTPGLVVSTGLFLLLRNITDVFSLAYWI
VVAVNALMALPYVVKTLSQPMWHLAQQYELLCASLGMSGWRRFRLVEWRALRKPLSHAFAIGFLLSMGDLSAIALFGSQD
FRTLPLYLYQLLGSYQMDTAAVVALAMLIMSVGWFYVIERLFAQRPLTKR

Sequences:

>Translated_530_residues
MNRTPQLGFWVAAGVATFVLAALAALLWQAPPLNLNLIWQDAYYRHVTQFSFYQAFWSTVLSVGLAIPVAHALSQRRFPG
RDLLLKLFAMTLVLPVLVAIFGLIAIYGNSGFLAKTLHSVGLSLPFSIYGLNGILLAHVLFNLPYASRLLLQSLEAIPSE
QHKLCAHLGMSRWQKFKWVEWPRLRQQLPHVAGLVFMLCFTSFATVMALGGGPKATTIELAIYQAIKFDFDLQIGALLAI
WQMLLCTLLTFTLQRFSHSRPMNSSGSKVTERWVRDSWRAKVWDTSWIVAACVLVLPPLVMVVLAGLNSKLGKVLTSGDF
WSAVSHSLQVASLASSLALASGIAMLLTSRALRLKAAHRRANGVEWIATLILVTPGLVVSTGLFLLLRNITDVFSLAYWI
VVAVNALMALPYVVKTLSQPMWHLAQQYELLCASLGMSGWRRFRLVEWRALRKPLSHAFAIGFLLSMGDLSAIALFGSQD
FRTLPLYLYQLLGSYQMDTAAVVALAMLIMSVGWFYVIERLFAQRPLTKR
>Mature_530_residues
MNRTPQLGFWVAAGVATFVLAALAALLWQAPPLNLNLIWQDAYYRHVTQFSFYQAFWSTVLSVGLAIPVAHALSQRRFPG
RDLLLKLFAMTLVLPVLVAIFGLIAIYGNSGFLAKTLHSVGLSLPFSIYGLNGILLAHVLFNLPYASRLLLQSLEAIPSE
QHKLCAHLGMSRWQKFKWVEWPRLRQQLPHVAGLVFMLCFTSFATVMALGGGPKATTIELAIYQAIKFDFDLQIGALLAI
WQMLLCTLLTFTLQRFSHSRPMNSSGSKVTERWVRDSWRAKVWDTSWIVAACVLVLPPLVMVVLAGLNSKLGKVLTSGDF
WSAVSHSLQVASLASSLALASGIAMLLTSRALRLKAAHRRANGVEWIATLILVTPGLVVSTGLFLLLRNITDVFSLAYWI
VVAVNALMALPYVVKTLSQPMWHLAQQYELLCASLGMSGWRRFRLVEWRALRKPLSHAFAIGFLLSMGDLSAIALFGSQD
FRTLPLYLYQLLGSYQMDTAAVVALAMLIMSVGWFYVIERLFAQRPLTKR

Specific function: Part of the ABC transporter complex thiBPQ involved in thiamine import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1178

COG function: function code P; ABC-type Fe3+ transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

Organism=Escherichia coli, GI1786254, Length=496, Percent_Identity=49.5967741935484, Blast_Score=424, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005947 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 58969; Mature: 58969

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure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CHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; thiamine [Periplasm]; H2O [C]

Specific reaction: ATP + thiamine [Periplasm] + H2O = ADP + phosphate + thiamine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1630901; 9278503; 9535878 [H]