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The map label for this gene is tsf

Identifier: 147675519

GI number: 147675519

Start: 1967760

End: 1968602

Strand: Reverse

Name: tsf

Synonym: VC0395_A1850

Alternate gene names: 147675519

Gene position: 1968602-1967760 (Counterclockwise)

Preceding gene: 147673418

Following gene: 147674990

Centisome position: 65.1

GC content: 48.16

Gene sequence:

>843_bases
ATGGCTGTTACTGCTGCTCTAGTAAAAGAACTGCGCGAGCGTACTGGCGCAGGTATGATGGAATGTAAGAAAGCCCTTGT
TGAAACCAACGGTGACATCGAGCTGGCGATCGAGAACATGCGTAAGAGTGGTGCTGCTAAAGCTGCTAAGAAAGCTGGCA
ACATCGCTGCTGAAGGCACCATCATGATCAAAGAAGGCGAAGGCATTGCGGCTCTAGTTGAAGTCAACTGCCAAACTGAC
TTCGTAGCAAAAGACAGCAACTTCGTAGCATTTGCTAACCAAGTTACTGACGCTGCTCTCGCTTCTAAAGCCTCTGTAGA
AGAGCTGCAAGCACAATTCGAAGAAGCTCGTGTTGCTCTTGTTGCGAAAATTGGTGAGAACATCAACATCCGTCGCGTTC
AGTACGTTGAAGGCGAAGCTCTGGCGACTTATCGCCATGGCGACCGTATCGGTGTTGTCGTTGCTGGTTCTGCTGACGTT
GAAACTCTAAAACACGTTGCAATGCACGTAGCGGCTTCTCGCCCAGAATTCCTGACTCCAGACGACGTTCCTGCTGAAGT
GGTTGCTAAAGAACGTGAAGTTCAAGTTGGCATCGCAATGAACGAAGGCAAGTCAAAAGAAATCGCAGAGAAAATGGTTG
AAGGCCGTATGAAGAAATTCACTGGCGAAGTTTCTCTGACTGGTCAGCCATTCGTAATGGAGCCAAAGAAAACTGTTGGT
GAAATTCTGGCTGAGAAAGGCGCAACTGTTTCTGCATTCATCCGTCTAGAAGTAGGTGAAGGCATCGAGAAACAAGAAGG
CCTGAGCTTTGCTGAAGAAGTAGCACTGGCGCAAAAAGGTTAA

Upstream 100 bases:

>100_bases
CCATGATTGTGTAGCATAGCCCAAGTTATAGTTAGCATTGGGGGCCGATAATGTAGGCCCTCAATTTTTACCCAATCCAG
AATTAACTGAGGAATAGAGA

Downstream 100 bases:

>100_bases
TCCTAGCGCGATTGCTCAGAAAAAGACCGTGGCCATGGCTGCGGTCTTTTCACGATTAAGGTGTTGATTCGTTAATGATT
GAATCATAAAGCGTCGACTC

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MAVTAALVKELRERTGAGMMECKKALVETNGDIELAIENMRKSGAAKAAKKAGNIAAEGTIMIKEGEGIAALVEVNCQTD
FVAKDSNFVAFANQVTDAALASKASVEELQAQFEEARVALVAKIGENINIRRVQYVEGEALATYRHGDRIGVVVAGSADV
ETLKHVAMHVAASRPEFLTPDDVPAEVVAKEREVQVGIAMNEGKSKEIAEKMVEGRMKKFTGEVSLTGQPFVMEPKKTVG
EILAEKGATVSAFIRLEVGEGIEKQEGLSFAEEVALAQKG

Sequences:

>Translated_280_residues
MAVTAALVKELRERTGAGMMECKKALVETNGDIELAIENMRKSGAAKAAKKAGNIAAEGTIMIKEGEGIAALVEVNCQTD
FVAKDSNFVAFANQVTDAALASKASVEELQAQFEEARVALVAKIGENINIRRVQYVEGEALATYRHGDRIGVVVAGSADV
ETLKHVAMHVAASRPEFLTPDDVPAEVVAKEREVQVGIAMNEGKSKEIAEKMVEGRMKKFTGEVSLTGQPFVMEPKKTVG
EILAEKGATVSAFIRLEVGEGIEKQEGLSFAEEVALAQKG
>Mature_279_residues
AVTAALVKELRERTGAGMMECKKALVETNGDIELAIENMRKSGAAKAAKKAGNIAAEGTIMIKEGEGIAALVEVNCQTDF
VAKDSNFVAFANQVTDAALASKASVEELQAQFEEARVALVAKIGENINIRRVQYVEGEALATYRHGDRIGVVVAGSADVE
TLKHVAMHVAASRPEFLTPDDVPAEVVAKEREVQVGIAMNEGKSKEIAEKMVEGRMKKFTGEVSLTGQPFVMEPKKTVGE
ILAEKGATVSAFIRLEVGEGIEKQEGLSFAEEVALAQKG

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Homo sapiens, GI171846268, Length=292, Percent_Identity=24.6575342465753, Blast_Score=75, Evalue=5e-14,
Organism=Escherichia coli, GI1786366, Length=279, Percent_Identity=70.6093189964158, Blast_Score=374, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17561440, Length=292, Percent_Identity=26.027397260274, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_VIBC3 (A5F606)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F606
- STRING:   A5F606
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1850
- eggNOG:   COG0264
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- ProtClustDB:   PRK09377
- BioCyc:   VCHO345073:VC0395_A1850-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 29848; Mature: 29716

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVTAALVKELRERTGAGMMECKKALVETNGDIELAIENMRKSGAAKAAKKAGNIAAEGT
CCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEHHHHHCCHHHHHHHCCCEEECCE
IMIKEGEGIAALVEVNCQTDFVAKDSNFVAFANQVTDAALASKASVEELQAQFEEARVAL
EEEECCCCEEEEEEECCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAKIGENINIRRVQYVEGEALATYRHGDRIGVVVAGSADVETLKHVAMHVAASRPEFLTP
HHHHCCCCCEEEEEEECCCEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCC
DDVPAEVVAKEREVQVGIAMNEGKSKEIAEKMVEGRMKKFTGEVSLTGQPFVMEPKKTVG
CCCCHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEECCCCEEECCHHHHH
EILAEKGATVSAFIRLEVGEGIEKQEGLSFAEEVALAQKG
HHHHHCCCEEEEEEEEECCCCCCHHHCCCHHHHHHHHCCC
>Mature Secondary Structure 
AVTAALVKELRERTGAGMMECKKALVETNGDIELAIENMRKSGAAKAAKKAGNIAAEGT
CHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEHHHHHCCHHHHHHHCCCEEECCE
IMIKEGEGIAALVEVNCQTDFVAKDSNFVAFANQVTDAALASKASVEELQAQFEEARVAL
EEEECCCCEEEEEEECCCCCEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VAKIGENINIRRVQYVEGEALATYRHGDRIGVVVAGSADVETLKHVAMHVAASRPEFLTP
HHHHCCCCCEEEEEEECCCEEEEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCC
DDVPAEVVAKEREVQVGIAMNEGKSKEIAEKMVEGRMKKFTGEVSLTGQPFVMEPKKTVG
CCCCHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEECCCCEEECCHHHHH
EILAEKGATVSAFIRLEVGEGIEKQEGLSFAEEVALAQKG
HHHHHCCCEEEEEEEEECCCCCCHHHCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA