Click here to switch to the map view.

The map label for this gene is yfiC [C]

Identifier: 147675421

GI number: 147675421

Start: 197724

End: 198446

Strand: Reverse

Name: yfiC [C]

Synonym: VC0395_A0192

Alternate gene names: 147675421

Gene position: 198446-197724 (Counterclockwise)

Preceding gene: 147673112

Following gene: 147674594

Centisome position: 6.56

GC content: 48.55

Gene sequence:

>723_bases
ATGAAAACCAATAAATTAATCAATAAAAGCTTTCGCTTTAAACAATTCTCTATAGAAGAAGGCACATGCGGCATGCCAAT
CAGTACCGATGGCGTTTTACTCGGTAGCTGGGCATTTAGCCTCTCTCCAACGACGATTTTAGATATTGGCTGTGGTACAG
GGCTTTTGAGTTTAATGTGCGCGCAGCGCTTCCCTCATGCCCACATCACCGCTTTGGATATTGAGCAAAGTGCGTATCAA
GCGGCAGAGCACAATCGCCAGCAGAGTCCCTGGGCTGAACGCATCGAATGCCAACATGCCGATATTCTGCACTGGCAGCC
CAGCAAACGCTTTGCCGCAATCATTTGTAATCCTCCTTATTTCAACAGCGGAGAAACCGCTCAACACCAAGTCAGAGCGA
CGGCACGCCACACCATTAGCTTGCAGCACCAAGCCTTGATTGAGCGCATACCTCAGCTCCTCGAACCCGATGGCGTAGCG
AGTTTTATTCTGCCTAAAGCGGAAGGTGAAGATTTCATCGTCCTAGCCAAACAAGCTGGACTCTTTGTGGGGCGCTATTG
TCAGGTTCAACCAACCACGGAAAAACCTGTGCATCGTCTATTATTTGAGCTGCACCTATCCCCTTGTTTACCAGTAGAGA
CAAGACTGGTGATCCGTGAGCAGCAGGGTTACAGTGAGGCGTTTTGCCAGTTAACTCGCGATTTTTATCTGAAGATGTCG
TAA

Upstream 100 bases:

>100_bases
CTTTATAAGCTCATTTTTTCACCAATCAACAGAATAAAATATTATTAACCATCTTCAGAAGAGAGCTTTTCGCTTAACTC
TGGTTTGACCTAGAATCTCG

Downstream 100 bases:

>100_bases
TTAAAGATGTGATCTAAAGCGTGATCCATCTATAATGTGCGCCCTGTTTTAAGACAGAGCCTTTCAACGCTTGTGGAGTA
ATTATTTGTGATTAAAACCT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA m6A37 methyltransferase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKTNKLINKSFRFKQFSIEEGTCGMPISTDGVLLGSWAFSLSPTTILDIGCGTGLLSLMCAQRFPHAHITALDIEQSAYQ
AAEHNRQQSPWAERIECQHADILHWQPSKRFAAIICNPPYFNSGETAQHQVRATARHTISLQHQALIERIPQLLEPDGVA
SFILPKAEGEDFIVLAKQAGLFVGRYCQVQPTTEKPVHRLLFELHLSPCLPVETRLVIREQQGYSEAFCQLTRDFYLKMS

Sequences:

>Translated_240_residues
MKTNKLINKSFRFKQFSIEEGTCGMPISTDGVLLGSWAFSLSPTTILDIGCGTGLLSLMCAQRFPHAHITALDIEQSAYQ
AAEHNRQQSPWAERIECQHADILHWQPSKRFAAIICNPPYFNSGETAQHQVRATARHTISLQHQALIERIPQLLEPDGVA
SFILPKAEGEDFIVLAKQAGLFVGRYCQVQPTTEKPVHRLLFELHLSPCLPVETRLVIREQQGYSEAFCQLTRDFYLKMS
>Mature_240_residues
MKTNKLINKSFRFKQFSIEEGTCGMPISTDGVLLGSWAFSLSPTTILDIGCGTGLLSLMCAQRFPHAHITALDIEQSAYQ
AAEHNRQQSPWAERIECQHADILHWQPSKRFAAIICNPPYFNSGETAQHQVRATARHTISLQHQALIERIPQLLEPDGVA
SFILPKAEGEDFIVLAKQAGLFVGRYCQVQPTTEKPVHRLLFELHLSPCLPVETRLVIREQQGYSEAFCQLTRDFYLKMS

Specific function: Specifically methylates the adenine in position 37 of tRNA(Val) [H]

COG id: COG4123

COG function: function code R; Predicted O-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. tRNA (adenine-N(6)-)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082131, Length=242, Percent_Identity=38.4297520661157, Blast_Score=150, Evalue=7e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR007848
- InterPro:   IPR022882 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: =2.1.1.55 [H]

Molecular weight: Translated: 27064; Mature: 27064

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTNKLINKSFRFKQFSIEEGTCGMPISTDGVLLGSWAFSLSPTTILDIGCGTGLLSLMC
CCCCHHHCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCEEEECCCCHHHHHHHH
AQRFPHAHITALDIEQSAYQAAEHNRQQSPWAERIECQHADILHWQPSKRFAAIICNPPY
HHHCCCCEEEEEECHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEECCCCCEEEEEECCCC
FNSGETAQHQVRATARHTISLQHQALIERIPQLLEPDGVASFILPKAEGEDFIVLAKQAG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCC
LFVGRYCQVQPTTEKPVHRLLFELHLSPCLPVETRLVIREQQGYSEAFCQLTRDFYLKMS
EEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHEEECC
>Mature Secondary Structure
MKTNKLINKSFRFKQFSIEEGTCGMPISTDGVLLGSWAFSLSPTTILDIGCGTGLLSLMC
CCCCHHHCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCEEEECCCCHHHHHHHH
AQRFPHAHITALDIEQSAYQAAEHNRQQSPWAERIECQHADILHWQPSKRFAAIICNPPY
HHHCCCCEEEEEECHHHHHHHHHCCCCCCCHHHHHCCCCCCEEEECCCCCEEEEEECCCC
FNSGETAQHQVRATARHTISLQHQALIERIPQLLEPDGVASFILPKAEGEDFIVLAKQAG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEECCC
LFVGRYCQVQPTTEKPVHRLLFELHLSPCLPVETRLVIREQQGYSEAFCQLTRDFYLKMS
EEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEEEEECCCHHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]