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The map label for this gene is leuO [H]

Identifier: 147675366

GI number: 147675366

Start: 2219320

End: 2220285

Strand: Reverse

Name: leuO [H]

Synonym: VC0395_A2061

Alternate gene names: 147675366

Gene position: 2220285-2219320 (Counterclockwise)

Preceding gene: 147673059

Following gene: 147675212

Centisome position: 73.42

GC content: 47.72

Gene sequence:

>966_bases
ATGCAAATGTTAGATAAAAAAGACGCAATGAGTGCCATTGCGAGTTACCGCATGGAAAGCACCCTCCGAGGCGTAGACCT
CAATCTGTTAACCGTTTTTGATGCAGTAATGCAAGAGCAAAACATTACGCGAGCGGCACACAACTTAGGTATGTCACAGC
CTGCGGTCAGTAATGCCGTGGCGCGTTTAAAAGTGATGTTCAATGATGAGTTGTTTATGCGCCAAGGGCGTGGCATTCAG
CCAACCCAGCGTGCTCGCCAACTGTTTGGCCCGATCCGTCAAGCTTTGCAATTGATCCGTAACGAGCTACCGAGTTCAGT
TTTCACACCGGAAACCTCAACGCGTCTGTTTAAGCTCGCGATCTGCAGTCCTTGCGATTTACGCTTTGCTCCTCAGATCA
TGGCATCGATTGATGAATTAGCACCGAGTGTACAATTGCATTTGGATGCAGAGTTTGATCGCCAAATCGCAGAGCGCATG
CGTTATCAAGAAATTGATTTTGTGATTGATTACGCTCGATTTGATGATCAAGGTTTTTCAAGCACTGAGATTTTCCAAGA
TGAGTTGGTGGTGGTGGCATCAAAACTTCACCCTCATATCCAAGGAAGTATCACGGCCGAACAATTGAGTGCGGAAAAAC
ACGCAAAATTGTCCAAAGTGCATGGGCAGCGTAGTTTCTCTGAACTGGCGTACCGTGATTTTGATTGCCAGTCTTATTAC
GAAGGCACCAGCCTGAGCAATGTGTTGTATGTGGTCGGTCAGTCTGAACTGGTGACCGTTGCTCCACGTTGGATGGCGGA
AAATGCGGCCAATCGTGATGAGTTGCAAATCCTCGCTTTCCCATTTGCTGAGTCCAAAATCTCGGGTTATCTGAGTTGGC
ATGAGTCGAGTGAAAAAGACAAAGGCCATATCTGGCTGCGTGACCAACTGATGGTGATTTGTGGCGAAGTGGTCGCGAAT
CGCTGA

Upstream 100 bases:

>100_bases
CGCCATCCACCAAATATCTGGGCGGTAAATATTACAAACAGCGCTTATCTTTCCACTGGCGATTTATGCATATTTATCGC
TAGTGACGACACTGTGAGGC

Downstream 100 bases:

>100_bases
TCCTTTGTTCATTCTACGTTTCTAAAGCCTTGGGCAGTAAAAACTGTCCAAGGCTTTTTTGTTACAGAATGAGTTTTAAT
CGATAACTTTGACGTTAGTC

Product: leucine transcriptional activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MQMLDKKDAMSAIASYRMESTLRGVDLNLLTVFDAVMQEQNITRAAHNLGMSQPAVSNAVARLKVMFNDELFMRQGRGIQ
PTQRARQLFGPIRQALQLIRNELPSSVFTPETSTRLFKLAICSPCDLRFAPQIMASIDELAPSVQLHLDAEFDRQIAERM
RYQEIDFVIDYARFDDQGFSSTEIFQDELVVVASKLHPHIQGSITAEQLSAEKHAKLSKVHGQRSFSELAYRDFDCQSYY
EGTSLSNVLYVVGQSELVTVAPRWMAENAANRDELQILAFPFAESKISGYLSWHESSEKDKGHIWLRDQLMVICGEVVAN
R

Sequences:

>Translated_321_residues
MQMLDKKDAMSAIASYRMESTLRGVDLNLLTVFDAVMQEQNITRAAHNLGMSQPAVSNAVARLKVMFNDELFMRQGRGIQ
PTQRARQLFGPIRQALQLIRNELPSSVFTPETSTRLFKLAICSPCDLRFAPQIMASIDELAPSVQLHLDAEFDRQIAERM
RYQEIDFVIDYARFDDQGFSSTEIFQDELVVVASKLHPHIQGSITAEQLSAEKHAKLSKVHGQRSFSELAYRDFDCQSYY
EGTSLSNVLYVVGQSELVTVAPRWMAENAANRDELQILAFPFAESKISGYLSWHESSEKDKGHIWLRDQLMVICGEVVAN
R
>Mature_321_residues
MQMLDKKDAMSAIASYRMESTLRGVDLNLLTVFDAVMQEQNITRAAHNLGMSQPAVSNAVARLKVMFNDELFMRQGRGIQ
PTQRARQLFGPIRQALQLIRNELPSSVFTPETSTRLFKLAICSPCDLRFAPQIMASIDELAPSVQLHLDAEFDRQIAERM
RYQEIDFVIDYARFDDQGFSSTEIFQDELVVVASKLHPHIQGSITAEQLSAEKHAKLSKVHGQRSFSELAYRDFDCQSYY
EGTSLSNVLYVVGQSELVTVAPRWMAENAANRDELQILAFPFAESKISGYLSWHESSEKDKGHIWLRDQLMVICGEVVAN
R

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081684, Length=295, Percent_Identity=53.5593220338983, Blast_Score=317, Evalue=5e-88,
Organism=Escherichia coli, GI87081766, Length=286, Percent_Identity=24.8251748251748, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1786820, Length=288, Percent_Identity=22.5694444444444, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: 825 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36402; Mature: 36402

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMLDKKDAMSAIASYRMESTLRGVDLNLLTVFDAVMQEQNITRAAHNLGMSQPAVSNAV
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ARLKVMFNDELFMRQGRGIQPTQRARQLFGPIRQALQLIRNELPSSVFTPETSTRLFKLA
HHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
ICSPCDLRFAPQIMASIDELAPSVQLHLDAEFDRQIAERMRYQEIDFVIDYARFDDQGFS
ECCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHEEEHHHHCCCCCC
STEIFQDELVVVASKLHPHIQGSITAEQLSAEKHAKLSKVHGQRSFSELAYRDFDCQSYY
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHC
EGTSLSNVLYVVGQSELVTVAPRWMAENAANRDELQILAFPFAESKISGYLSWHESSEKD
CCCCCCCEEEEECCCCEEEECCHHHHHCCCCCCCEEEEEECCHHHHHHHEEEECCCCCCC
KGHIWLRDQLMVICGEVVANR
CCEEEEHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQMLDKKDAMSAIASYRMESTLRGVDLNLLTVFDAVMQEQNITRAAHNLGMSQPAVSNAV
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ARLKVMFNDELFMRQGRGIQPTQRARQLFGPIRQALQLIRNELPSSVFTPETSTRLFKLA
HHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEE
ICSPCDLRFAPQIMASIDELAPSVQLHLDAEFDRQIAERMRYQEIDFVIDYARFDDQGFS
ECCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHEEEHHHHCCCCCC
STEIFQDELVVVASKLHPHIQGSITAEQLSAEKHAKLSKVHGQRSFSELAYRDFDCQSYY
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHC
EGTSLSNVLYVVGQSELVTVAPRWMAENAANRDELQILAFPFAESKISGYLSWHESSEKD
CCCCCCCEEEEECCCCEEEECCHHHHHCCCCCCCEEEEEECCHHHHHHHEEEECCCCCCC
KGHIWLRDQLMVICGEVVANR
CCEEEEHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3413113; 1630901; 9278503; 3519576 [H]