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The map label for this gene is pcm

Identifier: 147675360

GI number: 147675360

Start: 53882

End: 54508

Strand: Direct

Name: pcm

Synonym: VC0395_A0060

Alternate gene names: 147675360

Gene position: 53882-54508 (Clockwise)

Preceding gene: 147674427

Following gene: 147673872

Centisome position: 1.78

GC content: 51.04

Gene sequence:

>627_bases
ATGGCTAACCCAAAAGCCGACAGACTGATTCAATTTTTAACTGAGCAGGGGATTACCTCACCACAAGTGTTGGCGGCAAT
CCATGCACTGCCACGCGAGTTTTTCGTCGCACCGGCCATGATGCATCAAGCTTATGACAATAATGCCCTGCCCATAGGTC
AAGGCCAGACCATCTCGCAACCCTATATCGTGGCGAAGATGACAGAGCTACTGGCGTTAACCCCAGAAACCAAAGTGCTA
GAAATTGGTACGGGCTCTGGTTATCAAACCGCAGTGCTCGCCAAACTGGTTAATCATGTGTTTACTGTTGAGCGGATCAA
AACCCTGCAATGGGATGCGAAAAGACGCTTGAAACAGTTGGATATCTATAACGTGTCGACGAAACATGGCGATGGCTGGC
AAGGTTGGCCCGCTCGTGGACCTTTTGATGCGATCCTTGTGACGGCTGCTGCGGCAAAAGTGCCGCAAAGTCTATTGGAT
CAGCTTGCTGAGGGTGGTCGTATGGTGATCCCTGTGGGCGAAGACGAGCAATATCTGTACAAAATTGTGCGTCAAGGTGG
GCAGTTTATCTCTGAGCGGGTTGAAGCTGTGCGTTTTGTGCCACTGGTCGCGGGAGATCTTGCCTGA

Upstream 100 bases:

>100_bases
GCGATTGAACGTGGCTGGGTGTCACTCACGCCGCTACAAGTTGACCTAACGGCACATGAGTCACTGCGTAGCATGGATCA
TTGGTTAAAGGAAAAGGTGA

Downstream 100 bases:

>100_bases
TGACGCTTTACTCTTTCCGACTGGGCTTATTATTGTTTTGCAGCTTATTATTCGGCTGTACTGCACCTACGCCAGCACCA
GTCTCTGGATTGGGTAAAGA

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MANPKADRLIQFLTEQGITSPQVLAAIHALPREFFVAPAMMHQAYDNNALPIGQGQTISQPYIVAKMTELLALTPETKVL
EIGTGSGYQTAVLAKLVNHVFTVERIKTLQWDAKRRLKQLDIYNVSTKHGDGWQGWPARGPFDAILVTAAAAKVPQSLLD
QLAEGGRMVIPVGEDEQYLYKIVRQGGQFISERVEAVRFVPLVAGDLA

Sequences:

>Translated_208_residues
MANPKADRLIQFLTEQGITSPQVLAAIHALPREFFVAPAMMHQAYDNNALPIGQGQTISQPYIVAKMTELLALTPETKVL
EIGTGSGYQTAVLAKLVNHVFTVERIKTLQWDAKRRLKQLDIYNVSTKHGDGWQGWPARGPFDAILVTAAAAKVPQSLLD
QLAEGGRMVIPVGEDEQYLYKIVRQGGQFISERVEAVRFVPLVAGDLA
>Mature_207_residues
ANPKADRLIQFLTEQGITSPQVLAAIHALPREFFVAPAMMHQAYDNNALPIGQGQTISQPYIVAKMTELLALTPETKVLE
IGTGSGYQTAVLAKLVNHVFTVERIKTLQWDAKRRLKQLDIYNVSTKHGDGWQGWPARGPFDAILVTAAAAKVPQSLLDQ
LAEGGRMVIPVGEDEQYLYKIVRQGGQFISERVEAVRFVPLVAGDLA

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=209, Percent_Identity=33.4928229665072, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1789100, Length=208, Percent_Identity=64.4230769230769, Blast_Score=277, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI71983477, Length=172, Percent_Identity=36.046511627907, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI193207222, Length=142, Percent_Identity=38.0281690140845, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI17981723, Length=214, Percent_Identity=33.6448598130841, Blast_Score=91, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_VIBC3 (A5F9C1)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F9C1
- SMR:   A5F9C1
- STRING:   A5F9C1
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0060
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   MEEVPRE
- ProtClustDB:   PRK00312
- BioCyc:   VCHO345073:VC0395_A0060-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 22863; Mature: 22732

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 59-59

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANPKADRLIQFLTEQGITSPQVLAAIHALPREFFVAPAMMHQAYDNNALPIGQGQTISQ
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PYIVAKMTELLALTPETKVLEIGTGSGYQTAVLAKLVNHVFTVERIKTLQWDAKRRLKQL
CHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIYNVSTKHGDGWQGWPARGPFDAILVTAAAAKVPQSLLDQLAEGGRMVIPVGEDEQYLY
CEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHH
KIVRQGGQFISERVEAVRFVPLVAGDLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ANPKADRLIQFLTEQGITSPQVLAAIHALPREFFVAPAMMHQAYDNNALPIGQGQTISQ
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
PYIVAKMTELLALTPETKVLEIGTGSGYQTAVLAKLVNHVFTVERIKTLQWDAKRRLKQL
CHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DIYNVSTKHGDGWQGWPARGPFDAILVTAAAAKVPQSLLDQLAEGGRMVIPVGEDEQYLY
CEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHH
KIVRQGGQFISERVEAVRFVPLVAGDLA
HHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA