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The map label for this gene is tnpR [H]

Identifier: 147675219

GI number: 147675219

Start: 2516604

End: 2517170

Strand: Reverse

Name: tnpR [H]

Synonym: VC0395_A2362

Alternate gene names: 147675219

Gene position: 2517170-2516604 (Counterclockwise)

Preceding gene: 147674449

Following gene: 147673405

Centisome position: 83.24

GC content: 43.92

Gene sequence:

>567_bases
ATGAAAATTGGCTATGCCCGTGTCTCAACAAATGATCAAGAGCTTCACCTACAACTCGACGCTCTAAATGAAATGGGGTG
TGACAAGATATACACTGAAAAAGCGTCTGGAAAAACTTTTAACCGCCCTGAGCTTGAAAAAGCACTCGACAACCTACGTG
AAGGAGATGTCTTCGTTGTATGGCGCTTAGATCGACTGGGACGAAGCCTGCCTCACCTTATCGAAACAGTTAACGGGTTA
AAAGAGCGAAACATTGGGTTTGTATCAATTGCGGAAGCAATTGATACTTCAAGCCCTGCGGGAGAACTGATCTTTCACAT
TTTCGGTGCTATCGCGCAATTTGAACGTTCTCTGATTAGCGAAAGAACCAAGGCAGGCTTAGCCGCAGCCAAAAGGCGCG
GGAAAAATGTAGGTAGAAAGCCAAGTATGTCTAGGAAAGACATGAGATTAGCTGCTGGTATGCTACTTAGCGGTTCAATC
ACCAAAACTGAAGTAGCAAAACATTTTGGTGTATCAAGACCGACCTTAGATAAGGCACTGAAAAACATATCTTTGGAGGA
AGAGTAA

Upstream 100 bases:

>100_bases
ATAAACAGATAAACCTTGACGATAGTGTAAAACAATATGTAAATAAACTGATGTAAAGTAACTATTGCTTTGTTTTACAC
CGTTTGGGAACCAACATACT

Downstream 100 bases:

>100_bases
AGATATGTTTTTAGCCACATTAGATGAATTAGTCTGAATGCAATCTGTCAGGTTGTCCTAAATCAGTTCTGCCCCAAAAT
GTGTTTAGATAGCTGCAAAC

Product: DNA-invertase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MKIGYARVSTNDQELHLQLDALNEMGCDKIYTEKASGKTFNRPELEKALDNLREGDVFVVWRLDRLGRSLPHLIETVNGL
KERNIGFVSIAEAIDTSSPAGELIFHIFGAIAQFERSLISERTKAGLAAAKRRGKNVGRKPSMSRKDMRLAAGMLLSGSI
TKTEVAKHFGVSRPTLDKALKNISLEEE

Sequences:

>Translated_188_residues
MKIGYARVSTNDQELHLQLDALNEMGCDKIYTEKASGKTFNRPELEKALDNLREGDVFVVWRLDRLGRSLPHLIETVNGL
KERNIGFVSIAEAIDTSSPAGELIFHIFGAIAQFERSLISERTKAGLAAAKRRGKNVGRKPSMSRKDMRLAAGMLLSGSI
TKTEVAKHFGVSRPTLDKALKNISLEEE
>Mature_188_residues
MKIGYARVSTNDQELHLQLDALNEMGCDKIYTEKASGKTFNRPELEKALDNLREGDVFVVWRLDRLGRSLPHLIETVNGL
KERNIGFVSIAEAIDTSSPAGELIFHIFGAIAQFERSLISERTKAGLAAAKRRGKNVGRKPSMSRKDMRLAAGMLLSGSI
TKTEVAKHFGVSRPTLDKALKNISLEEE

Specific function: Resolvase catalyzes the resolution (a site-specific recombination) of the cointegrated replicon to yield the final transposition products [H]

COG id: COG1961

COG function: function code L; Site-specific recombinases, DNA invertase Pin homologs

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the site-specific recombinase resolvase family [H]

Homologues:

Organism=Escherichia coli, GI1787404, Length=178, Percent_Identity=47.752808988764, Blast_Score=162, Evalue=1e-41,
Organism=Escherichia coli, GI1787638, Length=183, Percent_Identity=32.7868852459016, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787827, Length=183, Percent_Identity=32.7868852459016, Blast_Score=87, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR006118
- InterPro:   IPR006119
- InterPro:   IPR006120 [H]

Pfam domain/function: PF02796 HTH_7; PF00239 Resolvase [H]

EC number: NA

Molecular weight: Translated: 20838; Mature: 20838

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS00397 RECOMBINASES_1 ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIGYARVSTNDQELHLQLDALNEMGCDKIYTEKASGKTFNRPELEKALDNLREGDVFVV
CCCCCEEECCCCCCEEEEHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEE
WRLDRLGRSLPHLIETVNGLKERNIGFVSIAEAIDTSSPAGELIFHIFGAIAQFERSLIS
EEHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ERTKAGLAAAKRRGKNVGRKPSMSRKDMRLAAGMLLSGSITKTEVAKHFGVSRPTLDKAL
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHH
KNISLEEE
HHCCCCCC
>Mature Secondary Structure
MKIGYARVSTNDQELHLQLDALNEMGCDKIYTEKASGKTFNRPELEKALDNLREGDVFVV
CCCCCEEECCCCCCEEEEHHHHHHCCCHHHHHCCCCCCCCCCHHHHHHHHHHCCCCEEEE
WRLDRLGRSLPHLIETVNGLKERNIGFVSIAEAIDTSSPAGELIFHIFGAIAQFERSLIS
EEHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ERTKAGLAAAKRRGKNVGRKPSMSRKDMRLAAGMLLSGSITKTEVAKHFGVSRPTLDKAL
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHH
KNISLEEE
HHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2170815; 2555186 [H]