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The map label for this gene is zraS [H]

Identifier: 147675202

GI number: 147675202

Start: 642046

End: 643350

Strand: Direct

Name: zraS [H]

Synonym: VC0395_A0604

Alternate gene names: 147675202

Gene position: 642046-643350 (Clockwise)

Preceding gene: 147673721

Following gene: 147674873

Centisome position: 21.23

GC content: 46.59

Gene sequence:

>1305_bases
ATGGTAGACAAACTACGCACTTCTGGCGCAGCTATGCGAGAACAAGAGAATGATTGCCATATTCCTTTTGCTCTGTTGGC
CTTTATCAAGGTGCTGTTTTCCAATGTAAAGCCAAAGCGTAAGTTCGATAGTTTAATGACCACCATCAGCGATTTAGTGC
CCGGGTGTCAGATTGCGTTACTTGAACGTCAATCCGACGAGCACTGGCGTATTATTCAAACCAGTGGCTTGACAGTGGAG
ACTCGCTTCAAAACCGCATTACCTCCATTTATTGAAGATGAGTGGTACTGCATGCCGAATGTGCAAGCGGAAATGGTGTG
GCGAGAGCATTACGCACACTGTTTTCCTGACTGTCAGAGCGCCTTAGCTGCGAAGTTTCACACCGCCGAAAATCATTATC
TCCTGCTGATTATGCAGCCGATGTTGGATGGTTTTAATCATTCACAACGCGATGAGTTATATGCGCTATTTGATGTTACC
AAAAGTGCGCTTTACTCCATGCTGCAATTTCGCAAAACCGTTATCGATAAGCATCAGGTTCAGCAAGAAGAAAAATTGGC
GTCTTTAGGTAAGTTAGCCGCTGGCGTCGCCCATGAAATCAATAACCCGCTTGGGTTTGTGATGAGTAACTTCAGTTTCC
TGAATGAATACGTTGCTCAGATAAGGCAGCATCCACAAATTCAACCGATAGCCGACCCTAGGCTTCATGAGATGATCGCC
GATAGTGAAGCGATCTTGATGGAGAGCTTAGAGGGACTGTCGCGGATCAAAAATATCGTTTCGAGCCTCAATGTGTACTC
ACATACGCATGAAAATATGGCAGAAATTGATATGCGTGACGTACTGAGTTCGGCACTGGCTTTGATTATTGGTGATCTGA
AATACCGAGCTCAGTTTGTGTATCCCGCACCAGAAAAGCCTTATTACATTCGTGGCAGCTACAACAAGCTGCAACAAGTG
TTTATCAACTTGATTATTAATGCGGTGCAATCCGTCTCTGCAACCCAAGAAGGGGTCGTGCGTCTTGAGCTCTGTGAAGT
CCGTTGGCCGGGAGAAGGCATTCCACGCGTACAAGTGCTGATTGAAGACAATGGCAAAGGGATCAGTGCAGAGCACCTTG
AAAAAGTGTTTGATCCCTTTTTCACAACTAAAAAAGTTGGTGACGGAGCGGGCTTGGGGTTATCCGTGGCACGTGAAATC
ATCGAAGAACACCATGGCAAAATAAAGCTCTGTTCGACAGAAGGGGTCGGAACGCGAGTCTTTGTGACACTGCCACAAGC
GGTGCGTGCTCAAGGAGCCTTCTGA

Upstream 100 bases:

>100_bases
AAGTCGGTAAGGGGAGCGAATTCACCATTCGTTTACCGGTGAATCCCAGTGCCGAAGCGGTAGACAATACCGATGCCAAA
GCGATGTAAATGGGGGCATT

Downstream 100 bases:

>100_bases
TGCAAAGCAACCGTGAAATTCTGCTAGTGGATGATGAACCTGCGGTGTTGAATGCCTTAAAGCGCGAGCTGCGTCCTTAT
TTCCCCCATCTACATACCGC

Product: sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 434; Mature: 434

Protein sequence:

>434_residues
MVDKLRTSGAAMREQENDCHIPFALLAFIKVLFSNVKPKRKFDSLMTTISDLVPGCQIALLERQSDEHWRIIQTSGLTVE
TRFKTALPPFIEDEWYCMPNVQAEMVWREHYAHCFPDCQSALAAKFHTAENHYLLLIMQPMLDGFNHSQRDELYALFDVT
KSALYSMLQFRKTVIDKHQVQQEEKLASLGKLAAGVAHEINNPLGFVMSNFSFLNEYVAQIRQHPQIQPIADPRLHEMIA
DSEAILMESLEGLSRIKNIVSSLNVYSHTHENMAEIDMRDVLSSALALIIGDLKYRAQFVYPAPEKPYYIRGSYNKLQQV
FINLIINAVQSVSATQEGVVRLELCEVRWPGEGIPRVQVLIEDNGKGISAEHLEKVFDPFFTTKKVGDGAGLGLSVAREI
IEEHHGKIKLCSTEGVGTRVFVTLPQAVRAQGAF

Sequences:

>Translated_434_residues
MVDKLRTSGAAMREQENDCHIPFALLAFIKVLFSNVKPKRKFDSLMTTISDLVPGCQIALLERQSDEHWRIIQTSGLTVE
TRFKTALPPFIEDEWYCMPNVQAEMVWREHYAHCFPDCQSALAAKFHTAENHYLLLIMQPMLDGFNHSQRDELYALFDVT
KSALYSMLQFRKTVIDKHQVQQEEKLASLGKLAAGVAHEINNPLGFVMSNFSFLNEYVAQIRQHPQIQPIADPRLHEMIA
DSEAILMESLEGLSRIKNIVSSLNVYSHTHENMAEIDMRDVLSSALALIIGDLKYRAQFVYPAPEKPYYIRGSYNKLQQV
FINLIINAVQSVSATQEGVVRLELCEVRWPGEGIPRVQVLIEDNGKGISAEHLEKVFDPFFTTKKVGDGAGLGLSVAREI
IEEHHGKIKLCSTEGVGTRVFVTLPQAVRAQGAF
>Mature_434_residues
MVDKLRTSGAAMREQENDCHIPFALLAFIKVLFSNVKPKRKFDSLMTTISDLVPGCQIALLERQSDEHWRIIQTSGLTVE
TRFKTALPPFIEDEWYCMPNVQAEMVWREHYAHCFPDCQSALAAKFHTAENHYLLLIMQPMLDGFNHSQRDELYALFDVT
KSALYSMLQFRKTVIDKHQVQQEEKLASLGKLAAGVAHEINNPLGFVMSNFSFLNEYVAQIRQHPQIQPIADPRLHEMIA
DSEAILMESLEGLSRIKNIVSSLNVYSHTHENMAEIDMRDVLSSALALIIGDLKYRAQFVYPAPEKPYYIRGSYNKLQQV
FINLIINAVQSVSATQEGVVRLELCEVRWPGEGIPRVQVLIEDNGKGISAEHLEKVFDPFFTTKKVGDGAGLGLSVAREI
IEEHHGKIKLCSTEGVGTRVFVTLPQAVRAQGAF

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=328, Percent_Identity=29.8780487804878, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1788549, Length=263, Percent_Identity=30.4182509505703, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI145693157, Length=271, Percent_Identity=24.7232472324723, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081816, Length=265, Percent_Identity=27.1698113207547, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786600, Length=274, Percent_Identity=23.3576642335766, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786783, Length=262, Percent_Identity=22.5190839694656, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 49030; Mature: 49030

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVDKLRTSGAAMREQENDCHIPFALLAFIKVLFSNVKPKRKFDSLMTTISDLVPGCQIAL
CCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEE
LERQSDEHWRIIQTSGLTVETRFKTALPPFIEDEWYCMPNVQAEMVWREHYAHCFPDCQS
EECCCCCCEEEEEECCCEEEECHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHCHHHHH
ALAAKFHTAENHYLLLIMQPMLDGFNHSQRDELYALFDVTKSALYSMLQFRKTVIDKHQV
HHHHHHCCCCCCEEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQEEKLASLGKLAAGVAHEINNPLGFVMSNFSFLNEYVAQIRQHPQIQPIADPRLHEMIA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHC
DSEAILMESLEGLSRIKNIVSSLNVYSHTHENMAEIDMRDVLSSALALIIGDLKYRAQFV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
YPAPEKPYYIRGSYNKLQQVFINLIINAVQSVSATQEGVVRLELCEVRWPGEGIPRVQVL
ECCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCEEEEE
IEDNGKGISAEHLEKVFDPFFTTKKVGDGAGLGLSVAREIIEEHHGKIKLCSTEGVGTRV
EECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCEE
FVTLPQAVRAQGAF
EEECCHHHHCCCCC
>Mature Secondary Structure
MVDKLRTSGAAMREQENDCHIPFALLAFIKVLFSNVKPKRKFDSLMTTISDLVPGCQIAL
CCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEEE
LERQSDEHWRIIQTSGLTVETRFKTALPPFIEDEWYCMPNVQAEMVWREHYAHCFPDCQS
EECCCCCCEEEEEECCCEEEECHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHCHHHHH
ALAAKFHTAENHYLLLIMQPMLDGFNHSQRDELYALFDVTKSALYSMLQFRKTVIDKHQV
HHHHHHCCCCCCEEEEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QQEEKLASLGKLAAGVAHEINNPLGFVMSNFSFLNEYVAQIRQHPQIQPIADPRLHEMIA
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHC
DSEAILMESLEGLSRIKNIVSSLNVYSHTHENMAEIDMRDVLSSALALIIGDLKYRAQFV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
YPAPEKPYYIRGSYNKLQQVFINLIINAVQSVSATQEGVVRLELCEVRWPGEGIPRVQVL
ECCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCCCCEEEEE
IEDNGKGISAEHLEKVFDPFFTTKKVGDGAGLGLSVAREIIEEHHGKIKLCSTEGVGTRV
EECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCEE
FVTLPQAVRAQGAF
EEECCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]