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The map label for this gene is pyrF

Identifier: 147674982

GI number: 147674982

Start: 1613981

End: 1614676

Strand: Reverse

Name: pyrF

Synonym: VC0395_A1501

Alternate gene names: 147674982

Gene position: 1614676-1613981 (Counterclockwise)

Preceding gene: 147675765

Following gene: 147675397

Centisome position: 53.39

GC content: 48.56

Gene sequence:

>696_bases
ATGAACGACCCAAAAGTGATTGTTGCACTGGACTATGACAATCTGGCGGATGCGCTCGCGTTTGTGGACAAAATTGACCC
AAGCACATGTCGACTCAAAGTGGGTAAAGAGATGTTTACTCTATTTGGTCCTGATTTTGTCCGTGAGCTGCATAAACGAG
GTTTTTCTGTGTTTCTGGATCTTAAATTTCACGATATTCCGAATACCTGTTCAAAAGCGGTGAAAGCTGCCGCTGAGCTT
GGGGTTTGGATGGTGAATGTGCACGCGAGTGGTGGCGAGCGAATGATGGCAGCATCGCGTGAGATTCTGGAGCCGTATGG
TAAAGAGCGCCCACTGCTGATTGGTGTCACTGTATTGACCAGTATGGAATCTGCCGATTTACAAGGTATTGGCATCTTGA
GTGCGCCACAAGATCATGTGCTGCGATTAGCAACGCTCGCCAAAAATGCTGGTCTTGATGGTGTGGTATGCTCCGCACAA
GAGGCATCACTATTGAAGCAACATTTGGGGCGTGAATTCAAATTGGTGACGCCAGGAATTCGTCCAGCGGGTTCAGAGCA
AGGCGATCAACGCCGTATTATGACTCCAGCCCAAGCCATTGCCTCTGGTTCAGATTATCTCGTGATCGGTCGCCCTATCA
CTCAAGCCGCACATCCTGAAGTGGTTCTGGAAGAGATAAACAGCTCTTTAGTTTAG

Upstream 100 bases:

>100_bases
GCCAATCAGAGGCTTAGATGGTGAGTAATTAAAAATCAACAGTATGACGCAGCTATTTATGGCTGCTTTTTTTTAACTCG
ATATACAGATAGGAGAAACG

Downstream 100 bases:

>100_bases
CTTTACTAGATTGGATAAACTCAAGCCCAGTCACAGTACTGGGCTTTTTTAAAAAGGCTATTTAGGAATACCACTGTGAA
ACTTGAAATCGCTATCGGGA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MNDPKVIVALDYDNLADALAFVDKIDPSTCRLKVGKEMFTLFGPDFVRELHKRGFSVFLDLKFHDIPNTCSKAVKAAAEL
GVWMVNVHASGGERMMAASREILEPYGKERPLLIGVTVLTSMESADLQGIGILSAPQDHVLRLATLAKNAGLDGVVCSAQ
EASLLKQHLGREFKLVTPGIRPAGSEQGDQRRIMTPAQAIASGSDYLVIGRPITQAAHPEVVLEEINSSLV

Sequences:

>Translated_231_residues
MNDPKVIVALDYDNLADALAFVDKIDPSTCRLKVGKEMFTLFGPDFVRELHKRGFSVFLDLKFHDIPNTCSKAVKAAAEL
GVWMVNVHASGGERMMAASREILEPYGKERPLLIGVTVLTSMESADLQGIGILSAPQDHVLRLATLAKNAGLDGVVCSAQ
EASLLKQHLGREFKLVTPGIRPAGSEQGDQRRIMTPAQAIASGSDYLVIGRPITQAAHPEVVLEEINSSLV
>Mature_231_residues
MNDPKVIVALDYDNLADALAFVDKIDPSTCRLKVGKEMFTLFGPDFVRELHKRGFSVFLDLKFHDIPNTCSKAVKAAAEL
GVWMVNVHASGGERMMAASREILEPYGKERPLLIGVTVLTSMESADLQGIGILSAPQDHVLRLATLAKNAGLDGVVCSAQ
EASLLKQHLGREFKLVTPGIRPAGSEQGDQRRIMTPAQAIASGSDYLVIGRPITQAAHPEVVLEEINSSLV

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=229, Percent_Identity=69.8689956331878, Blast_Score=323, Evalue=7e-90,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_VIBC3 (A5F6Y1)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F6Y1
- SMR:   A5F6Y1
- STRING:   A5F6Y1
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1501
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   PRK00230
- BioCyc:   VCHO345073:VC0395_A1501-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 24973; Mature: 24973

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 62-62 BINDING 11-11 BINDING 33-33 BINDING 120-120 BINDING 181-181 BINDING 190-190 BINDING 210-210 BINDING 211-211

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDPKVIVALDYDNLADALAFVDKIDPSTCRLKVGKEMFTLFGPDFVRELHKRGFSVFLD
CCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEECCHHHHHHCCHHHHHHHHHCCCEEEEE
LKFHDIPNTCSKAVKAAAELGVWMVNVHASGGERMMAASREILEPYGKERPLLIGVTVLT
EEECCCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHH
SMESADLQGIGILSAPQDHVLRLATLAKNAGLDGVVCSAQEASLLKQHLGREFKLVTPGI
CCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHCCCEEEEECCCC
RPAGSEQGDQRRIMTPAQAIASGSDYLVIGRPITQAAHPEVVLEEINSSLV
CCCCCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNDPKVIVALDYDNLADALAFVDKIDPSTCRLKVGKEMFTLFGPDFVRELHKRGFSVFLD
CCCCCEEEEECCHHHHHHHHHHHCCCCCCEEEECCHHHHHHCCHHHHHHHHHCCCEEEEE
LKFHDIPNTCSKAVKAAAELGVWMVNVHASGGERMMAASREILEPYGKERPLLIGVTVLT
EEECCCCHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEHHHH
SMESADLQGIGILSAPQDHVLRLATLAKNAGLDGVVCSAQEASLLKQHLGREFKLVTPGI
CCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCEEECHHHHHHHHHHCCCEEEEECCCC
RPAGSEQGDQRRIMTPAQAIASGSDYLVIGRPITQAAHPEVVLEEINSSLV
CCCCCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA