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The map label for this gene is trmB

Identifier: 147674960

GI number: 147674960

Start: 2500

End: 3219

Strand: Direct

Name: trmB

Synonym: VC0395_A0005

Alternate gene names: 147674960

Gene position: 2500-3219 (Clockwise)

Preceding gene: NA

Following gene: 147674885

Centisome position: 0.08

GC content: 51.25

Gene sequence:

>720_bases
ATGAGCGAAGTCATCACCCAGGAATATACCGAAGATGGCAAAGTGCTGCGTCGTATTCGTAGCTTTGTTCGTCGTGAAGG
TCGTTTGACCAAAGGCCAGGAAGCCGCAATGAAAGAGTGCTGGCCAACCATGGGGATTGATTACCAGCCAGAGCTGTTGG
ATTGGCAACAGGTGTTTGGTAACGACAATCCGGTTGTGCTGGAGATTGGTTTTGGTATGGGCGCGTCCTTGGTCGAAATG
GCGAAAAACGCACCTGAGAAAAACTTCCTCGGCATTGAAGTACACAGCCCAGGTGTCGGTGCTTGTTTGGCTTCGGCGCG
TGAAGCGGGTGTCACTAACTTGCGTGTGATGTGCCATGACGCGGTGGAAGTGTTTGCACACATGATCCCAGATAACAGCC
TGCACACTTTGCAATTGTTCTTCCCAGATCCTTGGCACAAGAAGCGTCACCACAAGCGCCGTATCGTGCAGCTTGAATTT
GCTGAAATGGTACGCCAAAAGCTCATCATCGGCAGCGGTGTATTCCATATGGCAACTGACTGGGAAAACTACGCAGAACA
CATGGTTGAAGTGATGAATCAAGCACCCGGTTTTGCTAACTTGGCAACCGATGGCGATTACATCCCGCGCCCTGATGAGC
GCCCGCTGACTAAGTTTGAGCAGCGTGGTCACCGTTTAGGTCACGGAGTGTGGGATATTAAGTACCAACGCACTGCTTAA

Upstream 100 bases:

>100_bases
GTTTGAGGGCAGGATAGTGAACGGTCAGTAATAACACTTGCACCGTTGACGATTCTTTGGATAATTCCCGCTCTGATTTA
GTAATGTGCAGGCAATAGAA

Downstream 100 bases:

>100_bases
ATATCCCCTTCCTATTTGAAGCTGCAGCGGTGTTGGCTATGTTCATTCACCCCAATCACATAGTCTGTCTATGCTCATGG
GGATGAACTCACTTGCCGCC

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MSEVITQEYTEDGKVLRRIRSFVRREGRLTKGQEAAMKECWPTMGIDYQPELLDWQQVFGNDNPVVLEIGFGMGASLVEM
AKNAPEKNFLGIEVHSPGVGACLASAREAGVTNLRVMCHDAVEVFAHMIPDNSLHTLQLFFPDPWHKKRHHKRRIVQLEF
AEMVRQKLIIGSGVFHMATDWENYAEHMVEVMNQAPGFANLATDGDYIPRPDERPLTKFEQRGHRLGHGVWDIKYQRTA

Sequences:

>Translated_239_residues
MSEVITQEYTEDGKVLRRIRSFVRREGRLTKGQEAAMKECWPTMGIDYQPELLDWQQVFGNDNPVVLEIGFGMGASLVEM
AKNAPEKNFLGIEVHSPGVGACLASAREAGVTNLRVMCHDAVEVFAHMIPDNSLHTLQLFFPDPWHKKRHHKRRIVQLEF
AEMVRQKLIIGSGVFHMATDWENYAEHMVEVMNQAPGFANLATDGDYIPRPDERPLTKFEQRGHRLGHGVWDIKYQRTA
>Mature_238_residues
SEVITQEYTEDGKVLRRIRSFVRREGRLTKGQEAAMKECWPTMGIDYQPELLDWQQVFGNDNPVVLEIGFGMGASLVEMA
KNAPEKNFLGIEVHSPGVGACLASAREAGVTNLRVMCHDAVEVFAHMIPDNSLHTLQLFFPDPWHKKRHHKRRIVQLEFA
EMVRQKLIIGSGVFHMATDWENYAEHMVEVMNQAPGFANLATDGDYIPRPDERPLTKFEQRGHRLGHGVWDIKYQRTA

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=237, Percent_Identity=67.5105485232068, Blast_Score=348, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_VIBC3 (A5F9I8)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F9I8
- SMR:   A5F9I8
- STRING:   A5F9I8
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0005
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- ProtClustDB:   PRK00121
- BioCyc:   VCHO345073:VC0395_A0005-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 27312; Mature: 27180

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: BINDING 68-68 BINDING 93-93 BINDING 120-120 BINDING 143-143 BINDING 147-147 BINDING 180-180

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVITQEYTEDGKVLRRIRSFVRREGRLTKGQEAAMKECWPTMGIDYQPELLDWQQVFG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHC
NDNPVVLEIGFGMGASLVEMAKNAPEKNFLGIEVHSPGVGACLASAREAGVTNLRVMCHD
CCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHH
AVEVFAHMIPDNSLHTLQLFFPDPWHKKRHHKRRIVQLEFAEMVRQKLIIGSGVFHMATD
HHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCC
WENYAEHMVEVMNQAPGFANLATDGDYIPRPDERPLTKFEQRGHRLGHGVWDIKYQRTA
HHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHHHHHCCHHHCCCEEEEEEECCC
>Mature Secondary Structure 
SEVITQEYTEDGKVLRRIRSFVRREGRLTKGQEAAMKECWPTMGIDYQPELLDWQQVFG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHC
NDNPVVLEIGFGMGASLVEMAKNAPEKNFLGIEVHSPGVGACLASAREAGVTNLRVMCHD
CCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCHHHHHHHHH
AVEVFAHMIPDNSLHTLQLFFPDPWHKKRHHKRRIVQLEFAEMVRQKLIIGSGVFHMATD
HHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCC
WENYAEHMVEVMNQAPGFANLATDGDYIPRPDERPLTKFEQRGHRLGHGVWDIKYQRTA
HHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCHHHHHHCCHHHCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA