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The map label for this gene is budR [H]

Identifier: 147674953

GI number: 147674953

Start: 1252723

End: 1253601

Strand: Reverse

Name: budR [H]

Synonym: VC0395_A1190

Alternate gene names: 147674953

Gene position: 1253601-1252723 (Counterclockwise)

Preceding gene: 147675555

Following gene: 147673445

Centisome position: 41.45

GC content: 48.58

Gene sequence:

>879_bases
ATGGAATTTCGTTACATGAAGTACTTTGCGGCCGTCGCAGAGGCAAAACATTTCACGCGTGCGGCGGAGCAACTTGGCAT
AGCCCAGCCACCTTTAAGTCAGCAAATCAAAAAGTTAGAAGAAGAGCTTGGCGTCAATCTTTTCAAACGATTATCACGAG
GCGTTGAGTTGACGCCCGCCGGTGAGCTTTTTTATCAGGATACGACCAGCATTCTCCAACAAGTTGAACGAGCCAAAGAT
CGAGTTAGACAAAGGGCAAGGGGAGAGCATCTTGAACTGCGTTTAGGCTTTGCTGCCTCAACCGCCAGCAGTTCTGTGGT
ATTAGAAAAAGTGTGCCAACTGCATGAAACGATTCCAGATCTACAATTGATCGCCGTAGAACATCCGATGCCAGAGTTGG
CTAGAATGATCCAAGAAAAACAGTTGGATATCGCATTTATGCGTTTGCCTTGTTATGCCAGTGAAGCGCTACGGCGAAAG
ATATTATTTGATGACCCATTTGTGGCGGTGATCCCCGCCGTGCATACGTTAGCGGAAGTGGAAAAGATTCAGCTCATCCA
GCTTAACCATGAAAATGTATTGATGTTTCCACGAGAGGTCGGCCCGGCCCTGTACGACAATATTGAAGCCGCATTCAGCG
CAGCCGGTGTCAATATCGCGCGATCGTATGCTGCGCCACAGTTAAGAACGGCAATTACTATGGCGCAAGCTGGGTTTGGC
ATTGCGATCGTTCCTTTATCGCTCACAGATCATCTTGACCATTCGGTGGTGGTGAAGACGATTCAAGATATGCCGTTTGT
CAGTCAGGTTGCTATCGTTTCTGATGCCGGACATCACCACCCCTTACTCAATCGCTGCATTGCGTGTATGGGGGAGTGA

Upstream 100 bases:

>100_bases
TCTAATATGAATCTGTGTGTATATTTAGGTTGACTTCTTAGTGGCGCAAAATCCAATATAAATAAGATATATTTTGATAT
AGAAAATATATGGGTGGTTA

Downstream 100 bases:

>100_bases
ATACAGAAGCAACATTTATTTGCCATCATCGACCAAGTGAATGTGGTGGATCTGATTCGCTACCTATCCCAACGATCCAA
AACCACACTATGAAGGTGGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Bud operon transcriptional regulator [H]

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MEFRYMKYFAAVAEAKHFTRAAEQLGIAQPPLSQQIKKLEEELGVNLFKRLSRGVELTPAGELFYQDTTSILQQVERAKD
RVRQRARGEHLELRLGFAASTASSSVVLEKVCQLHETIPDLQLIAVEHPMPELARMIQEKQLDIAFMRLPCYASEALRRK
ILFDDPFVAVIPAVHTLAEVEKIQLIQLNHENVLMFPREVGPALYDNIEAAFSAAGVNIARSYAAPQLRTAITMAQAGFG
IAIVPLSLTDHLDHSVVVKTIQDMPFVSQVAIVSDAGHHHPLLNRCIACMGE

Sequences:

>Translated_292_residues
MEFRYMKYFAAVAEAKHFTRAAEQLGIAQPPLSQQIKKLEEELGVNLFKRLSRGVELTPAGELFYQDTTSILQQVERAKD
RVRQRARGEHLELRLGFAASTASSSVVLEKVCQLHETIPDLQLIAVEHPMPELARMIQEKQLDIAFMRLPCYASEALRRK
ILFDDPFVAVIPAVHTLAEVEKIQLIQLNHENVLMFPREVGPALYDNIEAAFSAAGVNIARSYAAPQLRTAITMAQAGFG
IAIVPLSLTDHLDHSVVVKTIQDMPFVSQVAIVSDAGHHHPLLNRCIACMGE
>Mature_292_residues
MEFRYMKYFAAVAEAKHFTRAAEQLGIAQPPLSQQIKKLEEELGVNLFKRLSRGVELTPAGELFYQDTTSILQQVERAKD
RVRQRARGEHLELRLGFAASTASSSVVLEKVCQLHETIPDLQLIAVEHPMPELARMIQEKQLDIAFMRLPCYASEALRRK
ILFDDPFVAVIPAVHTLAEVEKIQLIQLNHENVLMFPREVGPALYDNIEAAFSAAGVNIARSYAAPQLRTAITMAQAGFG
IAIVPLSLTDHLDHSVVVKTIQDMPFVSQVAIVSDAGHHHPLLNRCIACMGE

Specific function: Regulator of the budABC operon for 2,3-butanediol synthesis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=248, Percent_Identity=34.6774193548387, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1788887, Length=294, Percent_Identity=32.6530612244898, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1790399, Length=245, Percent_Identity=32.6530612244898, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI2367136, Length=248, Percent_Identity=28.2258064516129, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI157672245, Length=199, Percent_Identity=32.1608040201005, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788297, Length=167, Percent_Identity=34.1317365269461, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787601, Length=140, Percent_Identity=31.4285714285714, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI145693105, Length=257, Percent_Identity=23.3463035019455, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI48994962, Length=146, Percent_Identity=31.5068493150685, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786401, Length=160, Percent_Identity=32.5, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87081904, Length=204, Percent_Identity=26.4705882352941, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1789639, Length=132, Percent_Identity=31.8181818181818, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1787806, Length=254, Percent_Identity=25.1968503937008, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI145693193, Length=126, Percent_Identity=30.1587301587302, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32543; Mature: 32543

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFRYMKYFAAVAEAKHFTRAAEQLGIAQPPLSQQIKKLEEELGVNLFKRLSRGVELTPA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCC
GELFYQDTTSILQQVERAKDRVRQRARGEHLELRLGFAASTASSSVVLEKVCQLHETIPD
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCC
LQLIAVEHPMPELARMIQEKQLDIAFMRLPCYASEALRRKILFDDPFVAVIPAVHTLAEV
EEEEEECCCCHHHHHHHHHHCCCHHEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
EKIQLIQLNHENVLMFPREVGPALYDNIEAAFSAAGVNIARSYAAPQLRTAITMAQAGFG
HHEEEEEECCCCEEEECHHHCHHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHCCCC
IAIVPLSLTDHLDHSVVVKTIQDMPFVSQVAIVSDAGHHHPLLNRCIACMGE
EEEEEEEHHHHCCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MEFRYMKYFAAVAEAKHFTRAAEQLGIAQPPLSQQIKKLEEELGVNLFKRLSRGVELTPA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCHHHHHHHHCCCCCCCC
GELFYQDTTSILQQVERAKDRVRQRARGEHLELRLGFAASTASSSVVLEKVCQLHETIPD
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCCC
LQLIAVEHPMPELARMIQEKQLDIAFMRLPCYASEALRRKILFDDPFVAVIPAVHTLAEV
EEEEEECCCCHHHHHHHHHHCCCHHEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
EKIQLIQLNHENVLMFPREVGPALYDNIEAAFSAAGVNIARSYAAPQLRTAITMAQAGFG
HHEEEEEECCCCEEEECHHHCHHHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHHHHCCCC
IAIVPLSLTDHLDHSVVVKTIQDMPFVSQVAIVSDAGHHHPLLNRCIACMGE
EEEEEEEHHHHCCHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA