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The map label for this gene is dltA [H]

Identifier: 147674686

GI number: 147674686

Start: 1242119

End: 1243789

Strand: Reverse

Name: dltA [H]

Synonym: VC0395_A1182

Alternate gene names: 147674686

Gene position: 1243789-1242119 (Counterclockwise)

Preceding gene: 147675267

Following gene: 147674196

Centisome position: 41.13

GC content: 45.84

Gene sequence:

>1671_bases
ATGCCATACAATTCTGCGACAGATTTGTTACAGAGAGAGGGTTATGGTGTCGTGCATAACCAATTTATCAATAACATGGC
GATGTACTGCGAGACAAGACACTCTATTTTGGCGGCTATTCAGGCTAACCGACATGCCCCTGCATTATGGGTTAAACAGA
AAACCTACACCTATCAAGAGATGACCGATATGGCACTCTCATTGAGTGACTATTGGCATTTACAGGGCGTGCAGCGAGTT
GCGATCCTTTCGGTGCGCGATCTCGCGGCTTATTCTGCGATATGGGCGAGCTATTTGGGCGGTATGACTTATATTCCGCT
CAATGCGCGAGCAACAACAGAACAGATCCAAGAGACGCTGATCGCGACACAATGTGATTCCATCATGGTAGACGCGCAGC
AATTGAGTCGTTTGTCCTCCTTACTAGAGACTTGCATTGACCGATTACACATTTATGCACTGCCTGATGTGGATGTGGAA
CCGTTACGTCAGCAATATCCTCAGCACACTTTTCACACAGTGCAAATAACCGAACAAGATGTGGAATTACTGGTAGTGAA
ATACCATTTAGACAATGAACATGAGTACGCTTATATCATGCAAACCTCAGGTTCGACTGGTAAACCCAAACGTATTGCGG
TCAGCTACAGTAATTTGCATTGCTATATCAGTCAAATTGACAAACTATTTCCACTCAATGCGCAAGATCGAGTGGGGCAA
TATTCTGATTTGACTTTTGATCTTTCGGTACATGATATTTTTTACAGTTTGATCTCTGGTGCTTGTTTATATGTTGTGCC
TGAATTGGCGAAATTAAGTCCAGCAGAATTTATTCGTCACCATCAATTAACGGTATGGCTTTCTGTGCCTACTGTTATTG
AGTTGGCCCTACAAAGACAAACCTTAACCCCACACAGTTTACCTTCTCTGCGACTGAGTTTTTTCTGCGGACAAGCCTTG
CTGCATGATTTAGCCGAACAATGGCAACAGGCCACCCAGCAGCCGGTGATCAATCTGTATGGTCCAACAGAGTGCACGAT
TGCCGTTACCTATCATCGATTTGTCGCCCATTCAGGTATGGCATCGGTGCCGATTGGTCGTGCTTTTGAAGAGGAGTGTT
TAGCCATCATCAACGAACAAGGCGAGTTGATGCGCTTTGAATCTGCACCCGAGGGGTATCGCGGAGAATTACTTCTGTCG
GGCAAACAGTTAGTGAAGGGTTATCTGAATGATCCGCTGAATACGCAAAGTGCCTTTTTCCAGCACGAAGGGCGTCTTTG
GTGTCGTTCAGGTGACATTGTCACTAAATCAAATGGCGTGCTGATTCATCTTGGGCGCCGCGATCACCAAGTGAAAATTG
CCGGACAACGGGTGGAATTAGAAGAGATTGAAACCGTTGTTCGCCGCGTGACTCAAGCGCACAGCGTGGCGATTGTGCCT
TGGCCTTTAAGTGAGTCGGGCTATGCCAGCGGCACGGTGGCGTTTGTGGATACCCATACCCAATGGCAACCAGATCTGTG
GTTGAGCCAATGCAAACAACAATTAAATCCGACTTTTGTGCCTAAGCGTTGGTATGCCATTGAACAATTACCAAGAAATG
CCAATGGCAAAACCGATATTAAAGCTTTACAACAACAATTAGCGAGTCAGACATATGAAACAAGCCATTGA

Upstream 100 bases:

>100_bases
TGATATGTCTATTGTCAATCGGTTGCTCGACAACTAGTTGCAATTATAGGTTGTAGACCTTCGCGCCATAGAAAGGAGGC
CGGGCGCTCGGTAGCTGACA

Downstream 100 bases:

>100_bases
AAATATCCTTATTGAGCGATTACAAACCAGTATTGAGGGTATTTCATCGATATTGTCGCCAAAGTAGAAAGCCAGTTTTC
GGCACAAATTAATCTATTTG

Product: enterobactin synthetase component F-related protein

Products: NA

Alternate protein names: D-alanine-D-alanyl carrier protein ligase; DCL; D-alanine-activating enzyme; DAE [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MPYNSATDLLQREGYGVVHNQFINNMAMYCETRHSILAAIQANRHAPALWVKQKTYTYQEMTDMALSLSDYWHLQGVQRV
AILSVRDLAAYSAIWASYLGGMTYIPLNARATTEQIQETLIATQCDSIMVDAQQLSRLSSLLETCIDRLHIYALPDVDVE
PLRQQYPQHTFHTVQITEQDVELLVVKYHLDNEHEYAYIMQTSGSTGKPKRIAVSYSNLHCYISQIDKLFPLNAQDRVGQ
YSDLTFDLSVHDIFYSLISGACLYVVPELAKLSPAEFIRHHQLTVWLSVPTVIELALQRQTLTPHSLPSLRLSFFCGQAL
LHDLAEQWQQATQQPVINLYGPTECTIAVTYHRFVAHSGMASVPIGRAFEEECLAIINEQGELMRFESAPEGYRGELLLS
GKQLVKGYLNDPLNTQSAFFQHEGRLWCRSGDIVTKSNGVLIHLGRRDHQVKIAGQRVELEEIETVVRRVTQAHSVAIVP
WPLSESGYASGTVAFVDTHTQWQPDLWLSQCKQQLNPTFVPKRWYAIEQLPRNANGKTDIKALQQQLASQTYETSH

Sequences:

>Translated_556_residues
MPYNSATDLLQREGYGVVHNQFINNMAMYCETRHSILAAIQANRHAPALWVKQKTYTYQEMTDMALSLSDYWHLQGVQRV
AILSVRDLAAYSAIWASYLGGMTYIPLNARATTEQIQETLIATQCDSIMVDAQQLSRLSSLLETCIDRLHIYALPDVDVE
PLRQQYPQHTFHTVQITEQDVELLVVKYHLDNEHEYAYIMQTSGSTGKPKRIAVSYSNLHCYISQIDKLFPLNAQDRVGQ
YSDLTFDLSVHDIFYSLISGACLYVVPELAKLSPAEFIRHHQLTVWLSVPTVIELALQRQTLTPHSLPSLRLSFFCGQAL
LHDLAEQWQQATQQPVINLYGPTECTIAVTYHRFVAHSGMASVPIGRAFEEECLAIINEQGELMRFESAPEGYRGELLLS
GKQLVKGYLNDPLNTQSAFFQHEGRLWCRSGDIVTKSNGVLIHLGRRDHQVKIAGQRVELEEIETVVRRVTQAHSVAIVP
WPLSESGYASGTVAFVDTHTQWQPDLWLSQCKQQLNPTFVPKRWYAIEQLPRNANGKTDIKALQQQLASQTYETSH
>Mature_555_residues
PYNSATDLLQREGYGVVHNQFINNMAMYCETRHSILAAIQANRHAPALWVKQKTYTYQEMTDMALSLSDYWHLQGVQRVA
ILSVRDLAAYSAIWASYLGGMTYIPLNARATTEQIQETLIATQCDSIMVDAQQLSRLSSLLETCIDRLHIYALPDVDVEP
LRQQYPQHTFHTVQITEQDVELLVVKYHLDNEHEYAYIMQTSGSTGKPKRIAVSYSNLHCYISQIDKLFPLNAQDRVGQY
SDLTFDLSVHDIFYSLISGACLYVVPELAKLSPAEFIRHHQLTVWLSVPTVIELALQRQTLTPHSLPSLRLSFFCGQALL
HDLAEQWQQATQQPVINLYGPTECTIAVTYHRFVAHSGMASVPIGRAFEEECLAIINEQGELMRFESAPEGYRGELLLSG
KQLVKGYLNDPLNTQSAFFQHEGRLWCRSGDIVTKSNGVLIHLGRRDHQVKIAGQRVELEEIETVVRRVTQAHSVAIVPW
PLSESGYASGTVAFVDTHTQWQPDLWLSQCKQQLNPTFVPKRWYAIEQLPRNANGKTDIKALQQQLASQTYETSH

Specific function: Involved in the biosynthesis of D-alanyl-lipoteichoic acid (LTA). Catalyzes an ATP-dependent two-step reaction where it forms a high energy D-alanyl AMP intermediate and transfers the alanyl residues from AMP to Dcp [H]

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. DltA subfamily [H]

Homologues:

Organism=Homo sapiens, GI45580730, Length=527, Percent_Identity=25.0474383301708, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI187761345, Length=532, Percent_Identity=23.8721804511278, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI187761343, Length=532, Percent_Identity=23.8721804511278, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI157311624, Length=538, Percent_Identity=20.631970260223, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI157311622, Length=538, Percent_Identity=20.631970260223, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI58082049, Length=541, Percent_Identity=21.0720887245841, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI42544132, Length=527, Percent_Identity=21.4421252371917, Blast_Score=73, Evalue=8e-13,
Organism=Homo sapiens, GI38505220, Length=361, Percent_Identity=19.9445983379501, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1786801, Length=542, Percent_Identity=30.2583025830258, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1788107, Length=539, Percent_Identity=22.0779220779221, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17556356, Length=380, Percent_Identity=26.5789473684211, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI32564420, Length=358, Percent_Identity=24.3016759776536, Blast_Score=69, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI32564422, Length=358, Percent_Identity=24.3016759776536, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319591, Length=412, Percent_Identity=27.1844660194175, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=373, Percent_Identity=21.71581769437, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648676, Length=391, Percent_Identity=26.3427109974425, Blast_Score=93, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010072 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.1.1.13 [H]

Molecular weight: Translated: 63090; Mature: 62959

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYNSATDLLQREGYGVVHNQFINNMAMYCETRHSILAAIQANRHAPALWVKQKTYTYQE
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHH
MTDMALSLSDYWHLQGVQRVAILSVRDLAAYSAIWASYLGGMTYIPLNARATTEQIQETL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHH
IATQCDSIMVDAQQLSRLSSLLETCIDRLHIYALPDVDVEPLRQQYPQHTFHTVQITEQD
HHHCCHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHCCCCCEEEEEEECCC
VELLVVKYHLDNEHEYAYIMQTSGSTGKPKRIAVSYSNLHCYISQIDKLFPLNAQDRVGQ
EEEEEEEEEECCCCCEEEEEEECCCCCCCCEEEEEECCHHHEEHHHHHHCCCCCHHHCCC
YSDLTFDLSVHDIFYSLISGACLYVVPELAKLSPAEFIRHHQLTVWLSVPTVIELALQRQ
CCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHCEEEEEEECHHHHHHHHHHC
TLTPHSLPSLRLSFFCGQALLHDLAEQWQQATQQPVINLYGPTECTIAVTYHRFVAHSGM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEEEHHHHHHCCC
ASVPIGRAFEEECLAIINEQGELMRFESAPEGYRGELLLSGKQLVKGYLNDPLNTQSAFF
CCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEECCHHHHHHHHCCCCCCHHHHH
QHEGRLWCRSGDIVTKSNGVLIHLGRRDHQVKIAGQRVELEEIETVVRRVTQAHSVAIVP
HCCCEEEEECCCEEEECCCEEEEECCCCCEEEEECCEECHHHHHHHHHHHHHHCCEEEEE
WPLSESGYASGTVAFVDTHTQWQPDLWLSQCKQQLNPTFVPKRWYAIEQLPRNANGKTDI
CCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHH
KALQQQLASQTYETSH
HHHHHHHHHHCCCCCC
>Mature Secondary Structure 
PYNSATDLLQREGYGVVHNQFINNMAMYCETRHSILAAIQANRHAPALWVKQKTYTYQE
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHH
MTDMALSLSDYWHLQGVQRVAILSVRDLAAYSAIWASYLGGMTYIPLNARATTEQIQETL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHH
IATQCDSIMVDAQQLSRLSSLLETCIDRLHIYALPDVDVEPLRQQYPQHTFHTVQITEQD
HHHCCHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHCCCCCEEEEEEECCC
VELLVVKYHLDNEHEYAYIMQTSGSTGKPKRIAVSYSNLHCYISQIDKLFPLNAQDRVGQ
EEEEEEEEEECCCCCEEEEEEECCCCCCCCEEEEEECCHHHEEHHHHHHCCCCCHHHCCC
YSDLTFDLSVHDIFYSLISGACLYVVPELAKLSPAEFIRHHQLTVWLSVPTVIELALQRQ
CCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHCEEEEEEECHHHHHHHHHHC
TLTPHSLPSLRLSFFCGQALLHDLAEQWQQATQQPVINLYGPTECTIAVTYHRFVAHSGM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEEEHHHHHHCCC
ASVPIGRAFEEECLAIINEQGELMRFESAPEGYRGELLLSGKQLVKGYLNDPLNTQSAFF
CCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEECCHHHHHHHHCCCCCCHHHHH
QHEGRLWCRSGDIVTKSNGVLIHLGRRDHQVKIAGQRVELEEIETVVRRVTQAHSVAIVP
HCCCEEEEECCCEEEECCCEEEEECCCCCEEEEECCEECHHHHHHHHHHHHHHCCEEEEE
WPLSESGYASGTVAFVDTHTQWQPDLWLSQCKQQLNPTFVPKRWYAIEQLPRNANGKTDI
CCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCHHH
KALQQQLASQTYETSH
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA