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The map label for this gene is hldE

Identifier: 147674683

GI number: 147674683

Start: 2165155

End: 2166585

Strand: Reverse

Name: hldE

Synonym: VC0395_A2014

Alternate gene names: 147674683

Gene position: 2166585-2165155 (Counterclockwise)

Preceding gene: 147673756

Following gene: 147673843

Centisome position: 71.64

GC content: 52.41

Gene sequence:

>1431_bases
ATGAAACCAGTGCTACCTGACTACAGCAAAGCGGGTGTGTTAATTGTCGGTGATGTCATGCTTGACCGTTACTGGTATGG
CCCAACGGGACGTATCTCGCCGGAAGCACCAGTACCTGTAGTGAAAGTAGAGCAGAGTGAAGAGCGTCCGGGCGGTGCCG
CTAACGTTGCGATGAACATCGCTTCTTTAGGTGGCCACGCGCACATCATTGGCCTGACAGGGCAAGATGAGCCGGCGAAT
GTGCTTGCCAACAAGCTCACCTCACTGAAAGTCCATTGTGATTTTGTGGCGCTGCCGGATTACCCGACCATCACTAAATT
GCGTGTATTGAGCCGCGGTCAGCAGTTGATCCGTCTGGATTTTGAAGACAAATTCGAAAACACGGATGCGCAGCTTATCT
TATCGCGCATGGAAAGTGCGCTACCTAAAGTGCGCGCGGTGATCCTGTCTGATTATGCGAAAGGTGCGTTAGAGCACGTA
CAGCAGTTTATTCAGAAAGCCAAGGCGGCTGGTGTACCTGTGTTTATCGATCCTAAGGGCAGCGACTTTGAACGCTATCG
TGGTGCATCGCTGCTTACGCCAAACATGTCTGAATTTGAAGCTGTGGTCGGCAAAGTCAAATCTGAGCAAGAGCTGGTTG
AAAAAGGTTTTGCCCTGATTGAAAAGTTCGATCTCGGCGCATTACTGGTGACTCGTAGCGAACACGGTATGACTCTGCTG
CGCCGTGGACTGGAACCTTTCCATTTACCCACCCAAGCCAAAGAAGTGTATGACGTGACAGGCGCCGGGGATACGGTGAT
TTCTGTGCTTGCGGCGTCGGTTGCGGCAGGTAAAGCGCTGGATGAAGCTTGCGCTCTGGCTAATGCTGCCGCCGGTGTGG
TGGTCGGTAAACTGGGTACGTCTACTGTTTCTACCATTGAATTGGCGGAAGCCGTGCACGGCAGCAAAGACACTGATTAT
GGCGTGATCAGCGAAGATGCTCTGATTGAAGCCGTGAAAAAAGCGCAAGCGCGTGGCGAAAAAGTGGTGATGACCAACGG
CTGCTTTGACATTTTGCACGCAGGCCATGTTTCTTACCTTAACCATGCGGCAGAGCTTGGCGATCGCTTAATCGTTGCGG
TGAACACCGATGAATCGGTGAAACGCTTGAAAGGCCCTGGCCGTCCGGTCAACAGTACCGATCGTCGTATGGCGGTATTG
GCGGGTCTTGGCGCGGTGGACTGGGTCGTGCCATTTAGCGAAGATACACCACAGCGTTTGATCGCAGCCATATTGCCAAA
CCTACTGGTGAAAGGTGGCGACTACAAACCGGAAGAGATTGCGGGTGGCAAAGAAGTGATTGCCGCGGGTGGTGAAGTCA
AAGTATTGAACTTTGAAGAAGGCTGCTCAACCACCGAAATCATCGAAGCGATCAAAGGTGGTCGTGGTTAA

Upstream 100 bases:

>100_bases
ACAAGTGATACAAGCTTGGCAGCAGTGGCTCGGTTGAGCACTTCTGCCACCTTGGCGGCTGTGATAAACTCCGCCCCAAT
CTGAATATTGGAGATTGAGA

Downstream 100 bases:

>100_bases
CGTGGATTGATAAGTAATGTGATACTTATCAATTACTTAATCGATATATAGCAAAAGGGGCCAAATGGCCCCTTCATTTA
TTTCATGCAGCTCAAAAGAG

Product: bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MKPVLPDYSKAGVLIVGDVMLDRYWYGPTGRISPEAPVPVVKVEQSEERPGGAANVAMNIASLGGHAHIIGLTGQDEPAN
VLANKLTSLKVHCDFVALPDYPTITKLRVLSRGQQLIRLDFEDKFENTDAQLILSRMESALPKVRAVILSDYAKGALEHV
QQFIQKAKAAGVPVFIDPKGSDFERYRGASLLTPNMSEFEAVVGKVKSEQELVEKGFALIEKFDLGALLVTRSEHGMTLL
RRGLEPFHLPTQAKEVYDVTGAGDTVISVLAASVAAGKALDEACALANAAAGVVVGKLGTSTVSTIELAEAVHGSKDTDY
GVISEDALIEAVKKAQARGEKVVMTNGCFDILHAGHVSYLNHAAELGDRLIVAVNTDESVKRLKGPGRPVNSTDRRMAVL
AGLGAVDWVVPFSEDTPQRLIAAILPNLLVKGGDYKPEEIAGGKEVIAAGGEVKVLNFEEGCSTTEIIEAIKGGRG

Sequences:

>Translated_476_residues
MKPVLPDYSKAGVLIVGDVMLDRYWYGPTGRISPEAPVPVVKVEQSEERPGGAANVAMNIASLGGHAHIIGLTGQDEPAN
VLANKLTSLKVHCDFVALPDYPTITKLRVLSRGQQLIRLDFEDKFENTDAQLILSRMESALPKVRAVILSDYAKGALEHV
QQFIQKAKAAGVPVFIDPKGSDFERYRGASLLTPNMSEFEAVVGKVKSEQELVEKGFALIEKFDLGALLVTRSEHGMTLL
RRGLEPFHLPTQAKEVYDVTGAGDTVISVLAASVAAGKALDEACALANAAAGVVVGKLGTSTVSTIELAEAVHGSKDTDY
GVISEDALIEAVKKAQARGEKVVMTNGCFDILHAGHVSYLNHAAELGDRLIVAVNTDESVKRLKGPGRPVNSTDRRMAVL
AGLGAVDWVVPFSEDTPQRLIAAILPNLLVKGGDYKPEEIAGGKEVIAAGGEVKVLNFEEGCSTTEIIEAIKGGRG
>Mature_476_residues
MKPVLPDYSKAGVLIVGDVMLDRYWYGPTGRISPEAPVPVVKVEQSEERPGGAANVAMNIASLGGHAHIIGLTGQDEPAN
VLANKLTSLKVHCDFVALPDYPTITKLRVLSRGQQLIRLDFEDKFENTDAQLILSRMESALPKVRAVILSDYAKGALEHV
QQFIQKAKAAGVPVFIDPKGSDFERYRGASLLTPNMSEFEAVVGKVKSEQELVEKGFALIEKFDLGALLVTRSEHGMTLL
RRGLEPFHLPTQAKEVYDVTGAGDTVISVLAASVAAGKALDEACALANAAAGVVVGKLGTSTVSTIELAEAVHGSKDTDY
GVISEDALIEAVKKAQARGEKVVMTNGCFDILHAGHVSYLNHAAELGDRLIVAVNTDESVKRLKGPGRPVNSTDRRMAVL
AGLGAVDWVVPFSEDTPQRLIAAILPNLLVKGGDYKPEEIAGGKEVIAAGGEVKVLNFEEGCSTTEIIEAIKGGRG

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family

Homologues:

Organism=Escherichia coli, GI1789432, Length=472, Percent_Identity=70.1271186440678, Blast_Score=670, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HLDE_VIBC3 (A5F5J2)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F5J2
- SMR:   A5F5J2
- STRING:   A5F5J2
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2014
- eggNOG:   COG2870
- HOGENOM:   HBG545535
- OMA:   LTPNRKE
- ProtClustDB:   PRK11316
- BioCyc:   VCHO345073:VC0395_A2014-MONOMER
- HAMAP:   MF_01603
- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00125
- TIGRFAMs:   TIGR02198
- TIGRFAMs:   TIGR02199

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB

EC number: 2.7.-.-

Molecular weight: Translated: 50661; Mature: 50661

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00583 PFKB_KINASES_1; PS00584 PFKB_KINASES_2

Important sites: ACT_SITE 264-264

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPVLPDYSKAGVLIVGDVMLDRYWYGPTGRISPEAPVPVVKVEQSEERPGGAANVAMNI
CCCCCCCCCCCCEEEEEHHHHHHEEECCCCCCCCCCCCCEEEECCCCCCCCCHHHHHEEH
ASLGGHAHIIGLTGQDEPANVLANKLTSLKVHCDFVALPDYPTITKLRVLSRGQQLIRLD
HHCCCCEEEEEECCCCCHHHHHHHHHHEEEEEEEEEECCCCCCHHHHHHHHCCCEEEEEC
FEDKFENTDAQLILSRMESALPKVRAVILSDYAKGALEHVQQFIQKAKAAGVPVFIDPKG
CHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
SDFERYRGASLLTPNMSEFEAVVGKVKSEQELVEKGFALIEKFDLGALLVTRSEHGMTLL
CCHHHHCCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHH
RRGLEPFHLPTQAKEVYDVTGAGDTVISVLAASVAAGKALDEACALANAAAGVVVGKLGT
HCCCCCCCCCCCHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCC
STVSTIELAEAVHGSKDTDYGVISEDALIEAVKKAQARGEKVVMTNGCFDILHAGHVSYL
CHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCHHHHH
NHAAELGDRLIVAVNTDESVKRLKGPGRPVNSTDRRMAVLAGLGAVDWVVPFSEDTPQRL
HHHHHCCCEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHEEEECCCCCHHHH
IAAILPNLLVKGGDYKPEEIAGGKEVIAAGGEVKVLNFEEGCSTTEIIEAIKGGRG
HHHHHHHHHCCCCCCCHHHHCCCCCEEECCCEEEEEECCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKPVLPDYSKAGVLIVGDVMLDRYWYGPTGRISPEAPVPVVKVEQSEERPGGAANVAMNI
CCCCCCCCCCCCEEEEEHHHHHHEEECCCCCCCCCCCCCEEEECCCCCCCCCHHHHHEEH
ASLGGHAHIIGLTGQDEPANVLANKLTSLKVHCDFVALPDYPTITKLRVLSRGQQLIRLD
HHCCCCEEEEEECCCCCHHHHHHHHHHEEEEEEEEEECCCCCCHHHHHHHHCCCEEEEEC
FEDKFENTDAQLILSRMESALPKVRAVILSDYAKGALEHVQQFIQKAKAAGVPVFIDPKG
CHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
SDFERYRGASLLTPNMSEFEAVVGKVKSEQELVEKGFALIEKFDLGALLVTRSEHGMTLL
CCHHHHCCCEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHH
RRGLEPFHLPTQAKEVYDVTGAGDTVISVLAASVAAGKALDEACALANAAAGVVVGKLGT
HCCCCCCCCCCCHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCC
STVSTIELAEAVHGSKDTDYGVISEDALIEAVKKAQARGEKVVMTNGCFDILHAGHVSYL
CHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHCCHHHHH
NHAAELGDRLIVAVNTDESVKRLKGPGRPVNSTDRRMAVLAGLGAVDWVVPFSEDTPQRL
HHHHHCCCEEEEEECCCHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHEEEECCCCCHHHH
IAAILPNLLVKGGDYKPEEIAGGKEVIAAGGEVKVLNFEEGCSTTEIIEAIKGGRG
HHHHHHHHHCCCCCCCHHHHCCCCCEEECCCEEEEEECCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA