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The map label for this gene is nudF [H]

Identifier: 147673843

GI number: 147673843

Start: 2162875

End: 2163501

Strand: Reverse

Name: nudF [H]

Synonym: VC0395_A2012

Alternate gene names: 147673843

Gene position: 2163501-2162875 (Counterclockwise)

Preceding gene: 147674683

Following gene: 147674226

Centisome position: 71.54

GC content: 46.09

Gene sequence:

>627_bases
ATGCAAGACGCGAATCAGCAGCCAGTCTCTTTTAATAAGAAAGATGTCGAAATCCTTAATAAAGAGACACTGTTTAAAGG
CTTTTTCCGCATGGTGAAGTACCGTTTTAAGCATAAACGTTTTGCGGGAGGCTGGAGTGAGCCTGTCGAGCGTGAAATGT
TTGAACGTGGTCATGCCGCAGCGATGTTGCCGTACGATCCTATTCGTGACCAAGTGGTGATCATCGAACAGATCCGCGTC
GGTGCGCTAGAACATGCACAGCCTTGGCAACTGGAAATTGTCGCTGGAGTGATTGATACCGATGAGAGTGCTGAACAAGT
CGTGCGCCGTGAAGCGGTAGAAGAAGCAGGGCTGACTGTAGGCAGAATTGAAAAAATTACCTCTTATTATCCTTCTTCTG
GGGGATGTTCTGAAAAATTGGATGTCTTTATAGGTGAAGTCGATTGTTCACAAGCGGGTGGCATTCATGGTTTAGACTGT
GAAGGAGAGGACATCAAAGTGCATGTGATGAGTCGGACTGAAGCGTATCAATTGGTCAAAGATGGGCGAATTGAAAATGG
GGCATCAATCATTGCCTTACAGTGGTTGGCGCTGAATTATCAGCCATTACAACAGCAATGGCAGTAA

Upstream 100 bases:

>100_bases
CTTAAAAAATAAGATAATTTTTATATAAAAAGTTGAGCCATCACTTGGTAGTCGGCAAATTCCACGAGTAAACTATAAAG
TTCATCAATAGGAGGTACGA

Downstream 100 bases:

>100_bases
CGAGTAATGAGTCAATTGACAGTTAAAAAAACGTATCATGTTGATCTCCCTGAATTAATGCGGGTGTATGAGACCAACTA
CGCCAAACTGAATGCTCTGC

Product: MutT/nudix family protein

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MQDANQQPVSFNKKDVEILNKETLFKGFFRMVKYRFKHKRFAGGWSEPVEREMFERGHAAAMLPYDPIRDQVVIIEQIRV
GALEHAQPWQLEIVAGVIDTDESAEQVVRREAVEEAGLTVGRIEKITSYYPSSGGCSEKLDVFIGEVDCSQAGGIHGLDC
EGEDIKVHVMSRTEAYQLVKDGRIENGASIIALQWLALNYQPLQQQWQ

Sequences:

>Translated_208_residues
MQDANQQPVSFNKKDVEILNKETLFKGFFRMVKYRFKHKRFAGGWSEPVEREMFERGHAAAMLPYDPIRDQVVIIEQIRV
GALEHAQPWQLEIVAGVIDTDESAEQVVRREAVEEAGLTVGRIEKITSYYPSSGGCSEKLDVFIGEVDCSQAGGIHGLDC
EGEDIKVHVMSRTEAYQLVKDGRIENGASIIALQWLALNYQPLQQQWQ
>Mature_208_residues
MQDANQQPVSFNKKDVEILNKETLFKGFFRMVKYRFKHKRFAGGWSEPVEREMFERGHAAAMLPYDPIRDQVVIIEQIRV
GALEHAQPWQLEIVAGVIDTDESAEQVVRREAVEEAGLTVGRIEKITSYYPSSGGCSEKLDVFIGEVDCSQAGGIHGLDC
EGEDIKVHVMSRTEAYQLVKDGRIENGASIIALQWLALNYQPLQQQWQ

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=208, Percent_Identity=54.3269230769231, Blast_Score=243, Evalue=6e-66,
Organism=Escherichia coli, GI1788810, Length=149, Percent_Identity=35.5704697986577, Blast_Score=88, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 23569; Mature: 23569

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDANQQPVSFNKKDVEILNKETLFKGFFRMVKYRFKHKRFAGGWSEPVEREMFERGHAA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCE
AMLPYDPIRDQVVIIEQIRVGALEHAQPWQLEIVAGVIDTDESAEQVVRREAVEEAGLTV
EECCCCCCCCCEEEEEHHHHCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCH
GRIEKITSYYPSSGGCSEKLDVFIGEVDCSQAGGIHGLDCEGEDIKVHVMSRTEAYQLVK
HHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHH
DGRIENGASIIALQWLALNYQPLQQQWQ
CCCCCCCCEEEEEEEHHCCCHHHHHCCC
>Mature Secondary Structure
MQDANQQPVSFNKKDVEILNKETLFKGFFRMVKYRFKHKRFAGGWSEPVEREMFERGHAA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCE
AMLPYDPIRDQVVIIEQIRVGALEHAQPWQLEIVAGVIDTDESAEQVVRREAVEEAGLTV
EECCCCCCCCCEEEEEHHHHCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHCCCH
GRIEKITSYYPSSGGCSEKLDVFIGEVDCSQAGGIHGLDCEGEDIKVHVMSRTEAYQLVK
HHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHH
DGRIENGASIIALQWLALNYQPLQQQWQ
CCCCCCCCEEEEEEEHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]