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The map label for this gene is gloA

Identifier: 147674604

GI number: 147674604

Start: 570637

End: 571053

Strand: Reverse

Name: gloA

Synonym: VC0395_A0531

Alternate gene names: 147674604

Gene position: 571053-570637 (Counterclockwise)

Preceding gene: 147674958

Following gene: 147675139

Centisome position: 18.88

GC content: 48.2

Gene sequence:

>417_bases
ATGTCCAACCATCGTATTTTGCACACCATGCTACGCGTCGGCGATTTGGATAAATCGATCGAATTCTATACTCAAGTGAT
GGGTATGAGCCTGCTGCGCAAAAATGAGAATACTGAGTACAAATACACCCTCGCCTTCTTAGGTTATGGTGATGAATCCC
AAGGTGCGGTCATTGAACTGACCTACAACTGGGGCGTGGCGGACTATGAGAAAGGCAACGCATACGGCCATATCGCGATT
GGGGTTGATGATATTTATGCGACTTGCGATACCATCAAAGCAGCGGGAGGCATTGTAACCCGTGAACCAGGCCCAGTGAA
AGGCGGGACAACTCACATCGCTTTCGTGAAAGACCCTGATGGTTACATGATTGAGCTTATCCAAAACAAGCAAGCCCATG
CTGGTCTTGAAGGCTAA

Upstream 100 bases:

>100_bases
GGCAGTTGCATTATCGAAGATCTTTGTGAATTCAAAGATAAAGTGTACCCAGAAAGCTAATTTCCCAAAAAACCGTTATC
CGGAAAGTAAGGAGCTCTTA

Downstream 100 bases:

>100_bases
TCGCCGATTCTGATGCATGATGCCGTCTTATGGACGGCATTTTTTTGCGCGCTGTTCTTTCACCCTTTCTCTACAATGCG
TAATTCCCACACAATTCCTG

Product: lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase

Number of amino acids: Translated: 138; Mature: 137

Protein sequence:

>138_residues
MSNHRILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIELTYNWGVADYEKGNAYGHIAI
GVDDIYATCDTIKAAGGIVTREPGPVKGGTTHIAFVKDPDGYMIELIQNKQAHAGLEG

Sequences:

>Translated_138_residues
MSNHRILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIELTYNWGVADYEKGNAYGHIAI
GVDDIYATCDTIKAAGGIVTREPGPVKGGTTHIAFVKDPDGYMIELIQNKQAHAGLEG
>Mature_137_residues
SNHRILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIELTYNWGVADYEKGNAYGHIAIG
VDDIYATCDTIKAAGGIVTREPGPVKGGTTHIAFVKDPDGYMIELIQNKQAHAGLEG

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family

Homologues:

Organism=Homo sapiens, GI118402586, Length=154, Percent_Identity=38.3116883116883, Blast_Score=99, Evalue=1e-21,
Organism=Escherichia coli, GI1787940, Length=132, Percent_Identity=68.1818181818182, Blast_Score=187, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17552228, Length=139, Percent_Identity=30.9352517985612, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323639, Length=140, Percent_Identity=41.4285714285714, Blast_Score=102, Evalue=2e-23,
Organism=Drosophila melanogaster, GI20129743, Length=146, Percent_Identity=39.7260273972603, Blast_Score=93, Evalue=6e-20,
Organism=Drosophila melanogaster, GI19920404, Length=143, Percent_Identity=34.2657342657343, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LGUL_VIBCH (Q9KT93)

Other databases:

- EMBL:   AE003852
- PIR:   H82251
- RefSeq:   NP_230656.1
- ProteinModelPortal:   Q9KT93
- SMR:   Q9KT93
- GeneID:   2614263
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1010
- TIGR:   VC_1010
- HOGENOM:   HBG704761
- OMA:   PGPMKHG
- ProtClustDB:   CLSK2484291
- BRENDA:   4.4.1.5
- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146
- TIGRFAMs:   TIGR00068

Pfam domain/function: PF00903 Glyoxalase

EC number: =4.4.1.5

Molecular weight: Translated: 15176; Mature: 15045

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00934 GLYOXALASE_I_1; PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNHRILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIEL
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEE
TYNWGVADYEKGNAYGHIAIGVDDIYATCDTIKAAGGIVTREPGPVKGGTTHIAFVKDPD
EECCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCC
GYMIELIQNKQAHAGLEG
CEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SNHRILHTMLRVGDLDKSIEFYTQVMGMSLLRKNENTEYKYTLAFLGYGDESQGAVIEL
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCEEEEE
TYNWGVADYEKGNAYGHIAIGVDDIYATCDTIKAAGGIVTREPGPVKGGTTHIAFVKDPD
EECCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCC
GYMIELIQNKQAHAGLEG
CEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301