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The map label for this gene is pilT-1 [H]

Identifier: 147674559

GI number: 147674559

Start: 9423

End: 10460

Strand: Direct

Name: pilT-1 [H]

Synonym: VC0395_A0014

Alternate gene names: 147674559

Gene position: 9423-10460 (Clockwise)

Preceding gene: 147674885

Following gene: 147675116

Centisome position: 0.31

GC content: 48.75

Gene sequence:

>1038_bases
ATGGATATCGCTGAGTTACTGGAATTTAGTGTAAAACATAACGCCTCAGATCTACATCTTTCCGCAGGTGTTCCGCCTAT
GGTACGGATTGATGGCGAAGTTAGGAAGCTTGGCGTGCCTGCTTTTACCCATTCTGATGTACATCGCTTGATTTTTGAGA
TCATGAATGATGCCCAGCGCAGTGAATATGAAGAGAAATTAGAAGTCGATTTTTCTTTTGAACTGCCTAATGTTGGCCGT
TTCCGGGTTAACGCGTTTCATCAAGCGCGCGGATGCTCGGCGGTGTTTCGTACCATTCCCACTGTCATCCCGACGCTAGA
GCAACTGGATGCTCCTGAAATCTTCAGCAAAATTGCCAATTATGAAAAAGGGTTAGTTTTAGTGACTGGGCCAACGGGCT
CGGGTAAGTCGACGACCTTGGCGGCGATGGTGAACTATGTCAATGCTCATCACAATAAGCATATTTTGACCATTGAAGAT
CCGATTGAATTTGTGCACAGCAACAATAAGTGTTTGATCAACCAACGCGAAGTGCACCGTGATACCCACAGTTTTAAAAA
TGCATTGCGCTCGGCATTGCGTGAAGACCCAGATGTGATCCTCGTCGGTGAGCTGCGTGACCAAGAAACCATTAGCTTGG
CGCTTACTGCGGCAGAAACCGGTCACTTGGTGTTTGGCACTCTACACACCAGCTCGGCGGCGAAAACTATTGACCGGATT
ATCGATGTTTTTCCGGGCAGCGACAAAGACATGGTGCGTTCCATGCTGTCGGAATCGCTGCGTGCGGTTATTGCGCAAAA
ACTCCTAAAACGCGTCGGGGGTGGCCGTGTCGCGTGTCATGAAATCATGCTGGCAACGCCAGCGATCCGGAACTTGATCC
GTGAAGATAAAGTGGCGCAGATGTATTCGATCATCCAAACCGGTGCCGCACACGGCATGCAAACCATGGAGCAAAACGCC
AAACAGCTGATCGCTCGTGGTGTGGTGGATGCGCAAGAAGTACAGAGCAAAATCGAGTTGGATTTAAAAGCATTTTAG

Upstream 100 bases:

>100_bases
AGCGCGTCCACACTTTTGTTGTGCGCTCTCAATCTGTGCAGTGATATGTTCAATGTTTTGTTGAATACTACGCATAGCCA
GACTTTATTAAGGAATTTAA

Downstream 100 bases:

>100_bases
GTAGGTAAAGACAGATGGAGTTGAATCAATATCTGGATAGCATGCTGACTCATAAAGCATCGGATCTGTACATTACGGTG
GGTGCACCGATTTTGTATCG

Product: twitching motility protein PilT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF

Sequences:

>Translated_345_residues
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF
>Mature_345_residues
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDAQRSEYEEKLEVDFSFELPNVGR
FRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIANYEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIED
PIEFVHSNNKCLINQREVHRDTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQMYSIIQTGAAHGMQTMEQNA
KQLIARGVVDAQEVQSKIELDLKAF

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=327, Percent_Identity=49.2354740061162, Blast_Score=317, Evalue=6e-88,
Organism=Escherichia coli, GI1789723, Length=349, Percent_Identity=32.6647564469914, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1786296, Length=378, Percent_Identity=29.1005291005291, Blast_Score=125, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38232; Mature: 38232

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDAQR
CCHHHHHHHHHHCCCCCEEEECCCCCEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHH
SEYEEKLEVDFSFELPNVGRFRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIAN
HHHHHHHEEEEEECCCCCCCEEHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
YEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIEDPIEFVHSNNKCLINQREVHR
CCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHCCCCEEECHHHHHH
DTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
HHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCEEEEEECCCHHHHHHHHH
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQ
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHH
MYSIIQTGAAHGMQTMEQNAKQLIARGVVDAQEVQSKIELDLKAF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHCCC
>Mature Secondary Structure
MDIAELLEFSVKHNASDLHLSAGVPPMVRIDGEVRKLGVPAFTHSDVHRLIFEIMNDAQR
CCHHHHHHHHHHCCCCCEEEECCCCCEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHH
SEYEEKLEVDFSFELPNVGRFRVNAFHQARGCSAVFRTIPTVIPTLEQLDAPEIFSKIAN
HHHHHHHEEEEEECCCCCCCEEHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
YEKGLVLVTGPTGSGKSTTLAAMVNYVNAHHNKHILTIEDPIEFVHSNNKCLINQREVHR
CCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHHHCCCCEEECHHHHHH
DTHSFKNALRSALREDPDVILVGELRDQETISLALTAAETGHLVFGTLHTSSAAKTIDRI
HHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCEEEEEECCCHHHHHHHHH
IDVFPGSDKDMVRSMLSESLRAVIAQKLLKRVGGGRVACHEIMLATPAIRNLIREDKVAQ
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHH
MYSIIQTGAAHGMQTMEQNAKQLIARGVVDAQEVQSKIELDLKAF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]