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The map label for this gene is kdsA [H]

Identifier: 147674359

GI number: 147674359

Start: 1873784

End: 1874635

Strand: Reverse

Name: kdsA [H]

Synonym: VC0395_A1752

Alternate gene names: 147674359

Gene position: 1874635-1873784 (Counterclockwise)

Preceding gene: 147674484

Following gene: 147673600

Centisome position: 61.99

GC content: 50.0

Gene sequence:

>852_bases
ATGGAACACAAAATTGTCCATGTCGGTGATATTCCGGTTGCCAACGATAAGCCGTTTACCCTATTTGCAGGGATGAACGT
ACTGGAATCGCGTGATTTGGCGATGCAAATTTGTGAGCACTACGTGAAAGTGACCGACAAGCTCGGCATCCCTTATGTGT
TCAAAGCATCGTTTGATAAAGCGAACCGCAGTTCAGTGCACTCTTACCGTGGCCCAGGCTTGGAAGAAGGGATGAAAATT
TTCCAAGAGCTGAAAGAGACCTTTGGCGTGAAGATCATCACCGATGTCCACACAGAAGCGCAAGCGCAGCCTGTGGCGGA
TGTGGTGGATGTGATTCAGCTACCTGCCTTTTTGGCCCGTCAAACCGACTTGGTTGAAGCGATGGCGAAAACCGGCGCGG
TCATTAACGTGAAAAAACCGCAGTTCATGAGCCCGGGTCAAGTTGGCAACATCGTTGAGAAGTTTGCTGAGTGTGGCAAT
GACAAAGTGATCTTATGTGAACGTGGCTCTTGCCACGGCTACGATAACCTCGTCGTCGATATGCTCGGCTTTGGTGTGAT
GAAGCAAGCTTCTAACGGCAGTCCAATCATTTTTGATGTGACTCACTCACTGCAAATGCGTGATCCATCAGGTGCGGCAT
CGGGCGGTCGTCGTGAGCAGACCGTTGAACTGGCGAAAGCCGGTTTGGCGACGGGCATTGCAGGTCTGTTTATTGAGGCG
CACCCAAATCCAGATAAAGCGCGTTGCGATGGCCCATCGGCATTACCTCTCGACAAATTAGAGCCATTCCTTGCGCAAAT
GAAAGCGCTGGATGATTTAATCAAAAGCTTTGCGCACATCGATATTCGATAA

Upstream 100 bases:

>100_bases
TGTCCGAACGATCCGGCGGCTGAATTGTTAAAAACGCAAGTCAATGCACTCAGTCACGACAGTCAAGTGACGCTGCATTA
ACCCAACAAGAGAGATTAAG

Downstream 100 bases:

>100_bases
GCGCGATAGCCTGACTCTATATTGTAAAGCCATCTTCGGATGGCTTTTTTCATGCCTAGCGCTTATCAATATGGCTCTGT
TTCAGCAAACGACGGTTTCT

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase [H]

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MEHKIVHVGDIPVANDKPFTLFAGMNVLESRDLAMQICEHYVKVTDKLGIPYVFKASFDKANRSSVHSYRGPGLEEGMKI
FQELKETFGVKIITDVHTEAQAQPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFMSPGQVGNIVEKFAECGN
DKVILCERGSCHGYDNLVVDMLGFGVMKQASNGSPIIFDVTHSLQMRDPSGAASGGRREQTVELAKAGLATGIAGLFIEA
HPNPDKARCDGPSALPLDKLEPFLAQMKALDDLIKSFAHIDIR

Sequences:

>Translated_283_residues
MEHKIVHVGDIPVANDKPFTLFAGMNVLESRDLAMQICEHYVKVTDKLGIPYVFKASFDKANRSSVHSYRGPGLEEGMKI
FQELKETFGVKIITDVHTEAQAQPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFMSPGQVGNIVEKFAECGN
DKVILCERGSCHGYDNLVVDMLGFGVMKQASNGSPIIFDVTHSLQMRDPSGAASGGRREQTVELAKAGLATGIAGLFIEA
HPNPDKARCDGPSALPLDKLEPFLAQMKALDDLIKSFAHIDIR
>Mature_283_residues
MEHKIVHVGDIPVANDKPFTLFAGMNVLESRDLAMQICEHYVKVTDKLGIPYVFKASFDKANRSSVHSYRGPGLEEGMKI
FQELKETFGVKIITDVHTEAQAQPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFMSPGQVGNIVEKFAECGN
DKVILCERGSCHGYDNLVVDMLGFGVMKQASNGSPIIFDVTHSLQMRDPSGAASGGRREQTVELAKAGLATGIAGLFIEA
HPNPDKARCDGPSALPLDKLEPFLAQMKALDDLIKSFAHIDIR

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family [H]

Homologues:

Organism=Escherichia coli, GI1787466, Length=281, Percent_Identity=80.0711743772242, Blast_Score=473, Evalue=1e-135,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269 [H]

Pfam domain/function: PF00793 DAHP_synth_1 [H]

EC number: =2.5.1.55 [H]

Molecular weight: Translated: 30774; Mature: 30774

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHKIVHVGDIPVANDKPFTLFAGMNVLESRDLAMQICEHYVKVTDKLGIPYVFKASFDK
CCCCEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
ANRSSVHSYRGPGLEEGMKIFQELKETFGVKIITDVHTEAQAQPVADVVDVIQLPAFLAR
CCHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEECCCHHCCCHHHHHHHHHHHHHHHHH
QTDLVEAMAKTGAVINVKKPQFMSPGQVGNIVEKFAECGNDKVILCERGSCHGYDNLVVD
HHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
MLGFGVMKQASNGSPIIFDVTHSLQMRDPSGAASGGRREQTVELAKAGLATGIAGLFIEA
HHHHHHHHHCCCCCCEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEE
HPNPDKARCDGPSALPLDKLEPFLAQMKALDDLIKSFAHIDIR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEHKIVHVGDIPVANDKPFTLFAGMNVLESRDLAMQICEHYVKVTDKLGIPYVFKASFDK
CCCCEEEECCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
ANRSSVHSYRGPGLEEGMKIFQELKETFGVKIITDVHTEAQAQPVADVVDVIQLPAFLAR
CCHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEECCCHHCCCHHHHHHHHHHHHHHHHH
QTDLVEAMAKTGAVINVKKPQFMSPGQVGNIVEKFAECGNDKVILCERGSCHGYDNLVVD
HHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHH
MLGFGVMKQASNGSPIIFDVTHSLQMRDPSGAASGGRREQTVELAKAGLATGIAGLFIEA
HHHHHHHHHCCCCCCEEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEE
HPNPDKARCDGPSALPLDKLEPFLAQMKALDDLIKSFAHIDIR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA