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The map label for this gene is ushA [H]

Identifier: 147673600

GI number: 147673600

Start: 1871711

End: 1873372

Strand: Reverse

Name: ushA [H]

Synonym: VC0395_A1751

Alternate gene names: 147673600

Gene position: 1873372-1871711 (Counterclockwise)

Preceding gene: 147674359

Following gene: 147675041

Centisome position: 61.95

GC content: 48.26

Gene sequence:

>1662_bases
ATGAAACAAGGCCTCATTCTAAAATCCGTCCTCAGTGCAGCCATTATTGCCTCTTTGGCAGGTTGTGCAACTGCACCCGC
TCAGCAGTGGGAAGCAGACAAAACCTACAAACTCACTATTCTGCACACCAACGACCACCATGGCCGTTTCTGGCAAAACC
AGTATGGTGAATATGGCATGGCAGCACGTAAAACGCTGATTGATCAACTGCGTGCCGACATTGAATCTCAAGGTGGCAGT
GTATTACTGCTCTCGGGGGGTGACATCAATACTGGGGTACCAGAATCAGACCTACAAGATGCAGAGCCTGATTTCAAAGG
TATGAGCAAAATCGGTTACGACGCTATGGCGCTGGGTAACCACGAATTTGATAATCCACTTGAAGTGCTGTTTAAGCAAA
AAGAGTGGGCGAACTTCCCAATGCTGTCGGCCAACATTTACGATAAAGCGACCGGTAAGCGTCTGTTTGAACCGTACCAT
ATCTTTGATAAGCAAGGGATTAAGATTGCGGTTATTGGCTTAACCACCGAAGACACCGCGAAAATTGGTAACCCAGAATA
CATCGGTGGTATCGACTTCCGCGATCCGAAAGAAGAAGCGAAGAAAGTGATCGCTGAGCTGAAGAAAAAAGAGAAGCCGG
ATCTTATCATCGCAGTAACGCACATGGGTCACTACCAAAACGGTGAGCATGGTGTGAACGCTCCGGGTGATGTTGCTTTG
GCGCGCTACTTGCCAGCGGGTGAGTTGGATATGATTGTCGGCGGTCACTCACAAGAGCCTGTGTGTATGGAAGGCCCTAA
TCTGGTGAAAAAGAACTTTAAGCCGGGTGACGAATGTAAGCCTGATATTCAAAACGGCACTTACATTGTGCAAGCTTACG
AGTGGGGTAAATACGTCGGTCGTGCGGATTATGAATTCCGTAACGGTGAGCTGAATATGGTCAGCTATAACCTCATTCCC
GTTAACTTGAAGAAGAAAGTTGAAGTGAATGGTGAAACTCAGCGTGTGTTTGCGACTTCTGAAATTAAAGAAGACAGCGC
AATGCTAGAGTTCCTGCGCCCATTCCAAGAGAAAGGCCAAGAGCAACTGAGCATCAAAATTGCCCACAGTAACGGTAAGC
TAGAAGGCGATCGTAACGTGGTGCGTTTTGAGCAAACCAACTTGGGCCGCATGATTGCAATGGCGCACATGCAACGTGCT
AAAGCCGATTTTGCGGTAATGAACTCTGGTGGCGTACGTGATTCTATCCAAGCCGGTGACATTACGTATAAAGATGTACT
GAAAGTGCAGCCATTTGGCAACATCGTCAGCTACGTGGATATGAATGGTCAGGAAGTGCTGGATTATCTCAATGTCGTCG
CTACCAAGCCGGTCGATTCAGGTGCGTATGCCCAGTTCGCAGGGATTTCAATGACGGTTGCGGATGGCAAAGTCTCTAAC
GTGGTCATCGGTGGCAAACAACTGCGTCTCGATGCGACCTACCGTTTCACTGTGCCAAGTTTTAACGCAGCGGGTGGTGA
CGGTTATCCAAAAATTACTGACCACCCAGGCTATGTCAACACTGGCTTTGTGGATGCCGAAGTACTGAAAGATTACCTTG
AGGCGAACAGTCCAATCGATGTGAACCGTTTCGCGCCAGCGGGCGAGATTGTTTATCGTTAA

Upstream 100 bases:

>100_bases
AAGCAATGTGCTCAAGCGATGTGCTGTGGTAAACGACTGCGCCGTGCGAGCGCCCAACGACATTTCAAGCAGTGGTTTTC
ATCAGTTCAAAGGTATGACA

Downstream 100 bases:

>100_bases
TAAGGTTTGACTCGCAAAGTTGAATTGCGTTTACTGACAGGCGGTGTGCAAATACCGCCTTTTTTGTTTTGCCATACCCA
TATGGCCCGAGCTCTGCCCA

Product: bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MKQGLILKSVLSAAIIASLAGCATAPAQQWEADKTYKLTILHTNDHHGRFWQNQYGEYGMAARKTLIDQLRADIESQGGS
VLLLSGGDINTGVPESDLQDAEPDFKGMSKIGYDAMALGNHEFDNPLEVLFKQKEWANFPMLSANIYDKATGKRLFEPYH
IFDKQGIKIAVIGLTTEDTAKIGNPEYIGGIDFRDPKEEAKKVIAELKKKEKPDLIIAVTHMGHYQNGEHGVNAPGDVAL
ARYLPAGELDMIVGGHSQEPVCMEGPNLVKKNFKPGDECKPDIQNGTYIVQAYEWGKYVGRADYEFRNGELNMVSYNLIP
VNLKKKVEVNGETQRVFATSEIKEDSAMLEFLRPFQEKGQEQLSIKIAHSNGKLEGDRNVVRFEQTNLGRMIAMAHMQRA
KADFAVMNSGGVRDSIQAGDITYKDVLKVQPFGNIVSYVDMNGQEVLDYLNVVATKPVDSGAYAQFAGISMTVADGKVSN
VVIGGKQLRLDATYRFTVPSFNAAGGDGYPKITDHPGYVNTGFVDAEVLKDYLEANSPIDVNRFAPAGEIVYR

Sequences:

>Translated_553_residues
MKQGLILKSVLSAAIIASLAGCATAPAQQWEADKTYKLTILHTNDHHGRFWQNQYGEYGMAARKTLIDQLRADIESQGGS
VLLLSGGDINTGVPESDLQDAEPDFKGMSKIGYDAMALGNHEFDNPLEVLFKQKEWANFPMLSANIYDKATGKRLFEPYH
IFDKQGIKIAVIGLTTEDTAKIGNPEYIGGIDFRDPKEEAKKVIAELKKKEKPDLIIAVTHMGHYQNGEHGVNAPGDVAL
ARYLPAGELDMIVGGHSQEPVCMEGPNLVKKNFKPGDECKPDIQNGTYIVQAYEWGKYVGRADYEFRNGELNMVSYNLIP
VNLKKKVEVNGETQRVFATSEIKEDSAMLEFLRPFQEKGQEQLSIKIAHSNGKLEGDRNVVRFEQTNLGRMIAMAHMQRA
KADFAVMNSGGVRDSIQAGDITYKDVLKVQPFGNIVSYVDMNGQEVLDYLNVVATKPVDSGAYAQFAGISMTVADGKVSN
VVIGGKQLRLDATYRFTVPSFNAAGGDGYPKITDHPGYVNTGFVDAEVLKDYLEANSPIDVNRFAPAGEIVYR
>Mature_553_residues
MKQGLILKSVLSAAIIASLAGCATAPAQQWEADKTYKLTILHTNDHHGRFWQNQYGEYGMAARKTLIDQLRADIESQGGS
VLLLSGGDINTGVPESDLQDAEPDFKGMSKIGYDAMALGNHEFDNPLEVLFKQKEWANFPMLSANIYDKATGKRLFEPYH
IFDKQGIKIAVIGLTTEDTAKIGNPEYIGGIDFRDPKEEAKKVIAELKKKEKPDLIIAVTHMGHYQNGEHGVNAPGDVAL
ARYLPAGELDMIVGGHSQEPVCMEGPNLVKKNFKPGDECKPDIQNGTYIVQAYEWGKYVGRADYEFRNGELNMVSYNLIP
VNLKKKVEVNGETQRVFATSEIKEDSAMLEFLRPFQEKGQEQLSIKIAHSNGKLEGDRNVVRFEQTNLGRMIAMAHMQRA
KADFAVMNSGGVRDSIQAGDITYKDVLKVQPFGNIVSYVDMNGQEVLDYLNVVATKPVDSGAYAQFAGISMTVADGKVSN
VVIGGKQLRLDATYRFTVPSFNAAGGDGYPKITDHPGYVNTGFVDAEVLKDYLEANSPIDVNRFAPAGEIVYR

Specific function: Degradation of extracellular 5'-nucleotides for nutritional needs [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=573, Percent_Identity=26.0034904013962, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI1786687, Length=549, Percent_Identity=62.1129326047359, Blast_Score=733, Evalue=0.0,
Organism=Escherichia coli, GI1790658, Length=494, Percent_Identity=21.4574898785425, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19922446, Length=538, Percent_Identity=27.3234200743494, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24654424, Length=538, Percent_Identity=27.3234200743494, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19922444, Length=581, Percent_Identity=25.1290877796902, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24641187, Length=579, Percent_Identity=24.3523316062176, Blast_Score=109, Evalue=6e-24,
Organism=Drosophila melanogaster, GI24652512, Length=541, Percent_Identity=25.3234750462107, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19921980, Length=541, Percent_Identity=25.3234750462107, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI161076508, Length=541, Percent_Identity=25.3234750462107, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28573524, Length=568, Percent_Identity=24.6478873239437, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI221329836, Length=573, Percent_Identity=23.5602094240838, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.5 [H]

Molecular weight: Translated: 60920; Mature: 60920

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQGLILKSVLSAAIIASLAGCATAPAQQWEADKTYKLTILHTNDHHGRFWQNQYGEYGM
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEEEEEECCCCCCHHHHHCCCCCH
AARKTLIDQLRADIESQGGSVLLLSGGDINTGVPESDLQDAEPDFKGMSKIGYDAMALGN
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECC
HEFDNPLEVLFKQKEWANFPMLSANIYDKATGKRLFEPYHIFDKQGIKIAVIGLTTEDTA
CCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCHHHCCCHHEECCCCCEEEEEEECCCCHH
KIGNPEYIGGIDFRDPKEEAKKVIAELKKKEKPDLIIAVTHMGHYQNGEHGVNAPGDVAL
CCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCHH
ARYLPAGELDMIVGGHSQEPVCMEGPNLVKKNFKPGDECKPDIQNGTYIVQAYEWGKYVG
HHHCCCCCEEEEECCCCCCCEEECCCHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCC
RADYEFRNGELNMVSYNLIPVNLKKKVEVNGETQRVFATSEIKEDSAMLEFLRPFQEKGQ
CCCCEEECCEEEEEEEEEEEECEEEEEEECCCCEEEEEEHHHCHHHHHHHHHHHHHHCCC
EQLSIKIAHSNGKLEGDRNVVRFEQTNLGRMIAMAHMQRAKADFAVMNSGGVRDSIQAGD
EEEEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCC
ITYKDVLKVQPFGNIVSYVDMNGQEVLDYLNVVATKPVDSGAYAQFAGISMTVADGKVSN
CCHHHHEEECCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEECCEEEEEECCCCCE
VVIGGKQLRLDATYRFTVPSFNAAGGDGYPKITDHPGYVNTGFVDAEVLKDYLEANSPID
EEECCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
VNRFAPAGEIVYR
CCCCCCCCCEEEC
>Mature Secondary Structure
MKQGLILKSVLSAAIIASLAGCATAPAQQWEADKTYKLTILHTNDHHGRFWQNQYGEYGM
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCEEEEEEEEECCCCCCHHHHHCCCCCH
AARKTLIDQLRADIESQGGSVLLLSGGDINTGVPESDLQDAEPDFKGMSKIGYDAMALGN
HHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEECC
HEFDNPLEVLFKQKEWANFPMLSANIYDKATGKRLFEPYHIFDKQGIKIAVIGLTTEDTA
CCCCCHHHHHHHHHHCCCCCEEECCCCCCCCCHHHCCCHHEECCCCCEEEEEEECCCCHH
KIGNPEYIGGIDFRDPKEEAKKVIAELKKKEKPDLIIAVTHMGHYQNGEHGVNAPGDVAL
CCCCCCEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCHH
ARYLPAGELDMIVGGHSQEPVCMEGPNLVKKNFKPGDECKPDIQNGTYIVQAYEWGKYVG
HHHCCCCCEEEEECCCCCCCEEECCCHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCC
RADYEFRNGELNMVSYNLIPVNLKKKVEVNGETQRVFATSEIKEDSAMLEFLRPFQEKGQ
CCCCEEECCEEEEEEEEEEEECEEEEEEECCCCEEEEEEHHHCHHHHHHHHHHHHHHCCC
EQLSIKIAHSNGKLEGDRNVVRFEQTNLGRMIAMAHMQRAKADFAVMNSGGVRDSIQAGD
EEEEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCC
ITYKDVLKVQPFGNIVSYVDMNGQEVLDYLNVVATKPVDSGAYAQFAGISMTVADGKVSN
CCHHHHEEECCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEECCEEEEEECCCCCE
VVIGGKQLRLDATYRFTVPSFNAAGGDGYPKITDHPGYVNTGFVDAEVLKDYLEANSPID
EEECCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCC
VNRFAPAGEIVYR
CCCCCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]