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The map label for this gene is moaA

Identifier: 147674357

GI number: 147674357

Start: 583866

End: 584870

Strand: Direct

Name: moaA

Synonym: VC0395_A0544

Alternate gene names: 147674357

Gene position: 583866-584870 (Clockwise)

Preceding gene: 147674934

Following gene: 147673390

Centisome position: 19.31

GC content: 47.76

Gene sequence:

>1005_bases
TTGGAAAGGTGTTCTGTGGCGCAACAATTCGAAGACAGATTTCAGCGTAAATTTTACTATTTACGTCTATCCATAACCGA
CGTTTGTAATTTCAAATGTACGTATTGCTTACCTGATGGTTACAAGCCATCAGCGGGTCGCCCTGCCTCTTTTCTGACGG
TGAATGAGATTCGCCGAGTGGTGAGTGCGTTTGCCCATTGTGGTACTTCCAAGGTCAGAATTACCGGCGGTGAACCTTCA
CTGCGCAAAGATTTTGGCGAAATCATTCATACCATAGCGCAAACTCCCGGCATTCAAAAAGTTGCGACGACCACCAATGG
CTACCGTTTAGCCAAACATATTGGTGAGTGGCGCGAAGCGGGGTTGACTCAACTGAATGTCAGCGTAGATAGCTTAGATC
CACGTATGTTTGCGCAAATCACCGGTGAAAACCGCTTTCAGCAAGTGATGTCCGGCATTGATCGCGCCTTTGAAGTTGGA
TTCGAGCAAGTCAAAGTCAACGTGGTACTGATGAAAAATCTTAACCACCTTGAACTGCCCCAATTTATGGCGTGGATTAA
AACTCGCCCCATCCAACTGCGTTTTATTGAGTTGATGCAAACCGGAGAAATGGACGCGCTGTTTGCTCGCCATCATGTTT
CTGGCATTTCGATCCGCGACTATCTCTTCGGTAACGGCTGGTTACTCAAAGCCAGAGCCGATAACGATGGCCCTGCACAG
GTGTTTATCCATCCGGATTTCCAAGGCGAGATTGGCCTTATCATGCCTTATGAGAAAGATTTTTGTCGCAGCTGTAACCG
CCTTCGGGTGTCAGCACTTGGCAAACTGCATCTGTGCTTATTTGGCGAACAAGGCATCGAGCTACGCGATCTTTTGCAAG
ACGATCACCAAGAAAACGCATTGATTGAACGAATTCAAACTCAGTTGCAAAACAAGGCGGAAACCCACTTCCTGCATGAT
GGAAATTCGGGTGTGACGCCGCATTTAGCCTCGATTGGCGGCTGA

Upstream 100 bases:

>100_bases
TCCGAGCTTACCGCGCTAAGTTTAAACCTTTAAATATGCGTTGTAAGCCAGTGACCGCTTGTCACAAGGGCAGAATTGGA
AATGATTTTGCCTCCCGTAT

Downstream 100 bases:

>100_bases
GTTTCTACCGATAATCTGACCTCACCATTGAGGTTTAACCATTTTGAAAAGGTGAACAAATGGGTCACGCAGAAAGCAAA
TTTCAAGCAGCAAACATCGC

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MERCSVAQQFEDRFQRKFYYLRLSITDVCNFKCTYCLPDGYKPSAGRPASFLTVNEIRRVVSAFAHCGTSKVRITGGEPS
LRKDFGEIIHTIAQTPGIQKVATTTNGYRLAKHIGEWREAGLTQLNVSVDSLDPRMFAQITGENRFQQVMSGIDRAFEVG
FEQVKVNVVLMKNLNHLELPQFMAWIKTRPIQLRFIELMQTGEMDALFARHHVSGISIRDYLFGNGWLLKARADNDGPAQ
VFIHPDFQGEIGLIMPYEKDFCRSCNRLRVSALGKLHLCLFGEQGIELRDLLQDDHQENALIERIQTQLQNKAETHFLHD
GNSGVTPHLASIGG

Sequences:

>Translated_334_residues
MERCSVAQQFEDRFQRKFYYLRLSITDVCNFKCTYCLPDGYKPSAGRPASFLTVNEIRRVVSAFAHCGTSKVRITGGEPS
LRKDFGEIIHTIAQTPGIQKVATTTNGYRLAKHIGEWREAGLTQLNVSVDSLDPRMFAQITGENRFQQVMSGIDRAFEVG
FEQVKVNVVLMKNLNHLELPQFMAWIKTRPIQLRFIELMQTGEMDALFARHHVSGISIRDYLFGNGWLLKARADNDGPAQ
VFIHPDFQGEIGLIMPYEKDFCRSCNRLRVSALGKLHLCLFGEQGIELRDLLQDDHQENALIERIQTQLQNKAETHFLHD
GNSGVTPHLASIGG
>Mature_334_residues
MERCSVAQQFEDRFQRKFYYLRLSITDVCNFKCTYCLPDGYKPSAGRPASFLTVNEIRRVVSAFAHCGTSKVRITGGEPS
LRKDFGEIIHTIAQTPGIQKVATTTNGYRLAKHIGEWREAGLTQLNVSVDSLDPRMFAQITGENRFQQVMSGIDRAFEVG
FEQVKVNVVLMKNLNHLELPQFMAWIKTRPIQLRFIELMQTGEMDALFARHHVSGISIRDYLFGNGWLLKARADNDGPAQ
VFIHPDFQGEIGLIMPYEKDFCRSCNRLRVSALGKLHLCLFGEQGIELRDLLQDDHQENALIERIQTQLQNKAETHFLHD
GNSGVTPHLASIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=309, Percent_Identity=36.2459546925566, Blast_Score=198, Evalue=5e-51,
Organism=Homo sapiens, GI22027536, Length=309, Percent_Identity=36.2459546925566, Blast_Score=198, Evalue=5e-51,
Organism=Escherichia coli, GI1786999, Length=329, Percent_Identity=63.5258358662614, Blast_Score=452, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI17561210, Length=320, Percent_Identity=36.5625, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI32566331, Length=311, Percent_Identity=37.2990353697749, Blast_Score=206, Evalue=1e-53,
Organism=Caenorhabditis elegans, GI32566329, Length=311, Percent_Identity=37.2990353697749, Blast_Score=205, Evalue=2e-53,
Organism=Drosophila melanogaster, GI28574490, Length=314, Percent_Identity=35.031847133758, Blast_Score=189, Evalue=3e-48,
Organism=Drosophila melanogaster, GI28574488, Length=312, Percent_Identity=35.2564102564103, Blast_Score=188, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_VIBC3 (A5F2Q5)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F2Q5
- SMR:   A5F2Q5
- STRING:   A5F2Q5
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0544
- eggNOG:   COG2896
- HOGENOM:   HBG529885
- OMA:   KHHVSGV
- ProtClustDB:   PRK00164
- BioCyc:   VCHO345073:VC0395_A0544-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 37858; Mature: 37858

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 22-22 BINDING 35-35 BINDING 73-73 BINDING 77-77 BINDING 104-104 BINDING 128-128 BINDING 165-165 BINDING 199-199

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERCSVAQQFEDRFQRKFYYLRLSITDVCNFKCTYCLPDGYKPSAGRPASFLTVNEIRRV
CCCCHHHHHHHHHHHHEEEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCEEEHHHHHHH
VSAFAHCGTSKVRITGGEPSLRKDFGEIIHTIAQTPGIQKVATTTNGYRLAKHIGEWREA
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHC
GLTQLNVSVDSLDPRMFAQITGENRFQQVMSGIDRAFEVGFEQVKVNVVLMKNLNHLELP
CCEEEEEECCCCCCHHEEEECCCHHHHHHHHHHHHHHHCCHHHEEEEEEEECCCCCCCHH
QFMAWIKTRPIQLRFIELMQTGEMDALFARHHVSGISIRDYLFGNGWLLKARADNDGPAQ
HHHHHHHCCCCCEEEEHHHHCCCHHHHHHHHHCCCCEEEHEEECCCEEEEEECCCCCCEE
VFIHPDFQGEIGLIMPYEKDFCRSCNRLRVSALGKLHLCLFGEQGIELRDLLQDDHQENA
EEECCCCCCCEEEEECCCHHHHHHHHHEEHHHCCEEEEEEECCCCCHHHHHHHCCCHHHH
LIERIQTQLQNKAETHFLHDGNSGVTPHLASIGG
HHHHHHHHHHCCHHHEEEECCCCCCCCCHHCCCC
>Mature Secondary Structure
MERCSVAQQFEDRFQRKFYYLRLSITDVCNFKCTYCLPDGYKPSAGRPASFLTVNEIRRV
CCCCHHHHHHHHHHHHEEEEEEEEEECCCCCEEEEECCCCCCCCCCCCCCEEEHHHHHHH
VSAFAHCGTSKVRITGGEPSLRKDFGEIIHTIAQTPGIQKVATTTNGYRLAKHIGEWREA
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHC
GLTQLNVSVDSLDPRMFAQITGENRFQQVMSGIDRAFEVGFEQVKVNVVLMKNLNHLELP
CCEEEEEECCCCCCHHEEEECCCHHHHHHHHHHHHHHHCCHHHEEEEEEEECCCCCCCHH
QFMAWIKTRPIQLRFIELMQTGEMDALFARHHVSGISIRDYLFGNGWLLKARADNDGPAQ
HHHHHHHCCCCCEEEEHHHHCCCHHHHHHHHHCCCCEEEHEEECCCEEEEEECCCCCCEE
VFIHPDFQGEIGLIMPYEKDFCRSCNRLRVSALGKLHLCLFGEQGIELRDLLQDDHQENA
EEECCCCCCCEEEEECCCHHHHHHHHHEEHHHCCEEEEEEECCCCCHHHHHHHCCCHHHH
LIERIQTQLQNKAETHFLHDGNSGVTPHLASIGG
HHHHHHHHHHCCHHHEEEECCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA