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The map label for this gene is cysD

Identifier: 147674260

GI number: 147674260

Start: 2294446

End: 2295354

Strand: Reverse

Name: cysD

Synonym: VC0395_A2137

Alternate gene names: 147674260

Gene position: 2295354-2294446 (Counterclockwise)

Preceding gene: 147675164

Following gene: 147673489

Centisome position: 75.9

GC content: 49.17

Gene sequence:

>909_bases
ATGGATCAACAGCGTTTAACCCATTTAAAACAGTTGGAAGCAGAAAGCATCCACATCATTCGTGAAGTGGCGGCCGAATT
TGATAATCCGGTGATGATGTACTCGATTGGCAAGGATTCATCGGTGATGTTGCATTTAACGCGCAAAGCGTTTTATCCGG
GCAAGATCCCTTTTCCTTTGTTGCACGTCGATACCGACTGGAAGTTTCGCGACATGATTACGTTTCGTGATGCGACGGCC
AAAAAGTACGGCTTTGATCTTATCGTGCATAAAAATCCAGAAGGTTTGGCGGCGGGGATCAACCCATTTGATCACGGCTC
CTCTAAGCATACCGACATCATGAAAACCCAAGGCTTGAAGCAGGCGTTGAACAAATATGGTTTTGATGCGGCCTTTGGTG
GTGCGCGTCGCGATGAAGAGAAATCGCGTGCCAAAGAGCGTGTCTACTCTTTTCGCGATAAAAACCACACTTGGGATCCG
AAAAACCAGCGCCCAGAGCTATGGCGCACCTATAACGGCCAGATCAACAAAGGTGAAAGCATTCGGGTGTTCCCGCTCTC
GAATTGGACGGAGCTGGATATCTGGCAATACATCTATTTGGAAAACATTGAGATTGTTCCGCTCTATCTGGCGGATGTCC
GCCCAGTCGTACAGCGTGATGGAATGCTGATCATGGTGGACGATGATCGGATGAAGCTGCGCGAAGGTGAACAGATTGAG
CACAAGAGCGTGCGTTTTCGCACCTTAGGCTGTTACCCACTGACTGGAGCCATCGAATCACAAGCCAATACATTGACCGA
GATTATTGAAGAGATGCTGGTGGCGACCTCCAGTGAGCGCCAAGGCCGCGCGATCGACCACGATCAGTCGGGATCAATGG
AACTGAAAAAACGTCAAGGTTACTTCTAA

Upstream 100 bases:

>100_bases
TGCCGCACTTATCTTCTTGGTCACTACTTAAAATTTCACACTACGGCGCTGCTTAAGGGCAGCGCGCCGCCGTCAGGCAA
GCGAAAAGGAAGCAGGTAAA

Downstream 100 bases:

>100_bases
GGATCGAAGGAAAGCGTATGAACAATGCAGTCAAAGAGCAGCTCGCTGAGCTCGGTATTGAAGGTTACCTCAATCAGCAT
CAACACAAATCGTTACTGAG

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MDQQRLTHLKQLEAESIHIIREVAAEFDNPVMMYSIGKDSSVMLHLTRKAFYPGKIPFPLLHVDTDWKFRDMITFRDATA
KKYGFDLIVHKNPEGLAAGINPFDHGSSKHTDIMKTQGLKQALNKYGFDAAFGGARRDEEKSRAKERVYSFRDKNHTWDP
KNQRPELWRTYNGQINKGESIRVFPLSNWTELDIWQYIYLENIEIVPLYLADVRPVVQRDGMLIMVDDDRMKLREGEQIE
HKSVRFRTLGCYPLTGAIESQANTLTEIIEEMLVATSSERQGRAIDHDQSGSMELKKRQGYF

Sequences:

>Translated_302_residues
MDQQRLTHLKQLEAESIHIIREVAAEFDNPVMMYSIGKDSSVMLHLTRKAFYPGKIPFPLLHVDTDWKFRDMITFRDATA
KKYGFDLIVHKNPEGLAAGINPFDHGSSKHTDIMKTQGLKQALNKYGFDAAFGGARRDEEKSRAKERVYSFRDKNHTWDP
KNQRPELWRTYNGQINKGESIRVFPLSNWTELDIWQYIYLENIEIVPLYLADVRPVVQRDGMLIMVDDDRMKLREGEQIE
HKSVRFRTLGCYPLTGAIESQANTLTEIIEEMLVATSSERQGRAIDHDQSGSMELKKRQGYF
>Mature_302_residues
MDQQRLTHLKQLEAESIHIIREVAAEFDNPVMMYSIGKDSSVMLHLTRKAFYPGKIPFPLLHVDTDWKFRDMITFRDATA
KKYGFDLIVHKNPEGLAAGINPFDHGSSKHTDIMKTQGLKQALNKYGFDAAFGGARRDEEKSRAKERVYSFRDKNHTWDP
KNQRPELWRTYNGQINKGESIRVFPLSNWTELDIWQYIYLENIEIVPLYLADVRPVVQRDGMLIMVDDDRMKLREGEQIE
HKSVRFRTLGCYPLTGAIESQANTLTEIIEEMLVATSSERQGRAIDHDQSGSMELKKRQGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily

Homologues:

Organism=Escherichia coli, GI1789109, Length=302, Percent_Identity=81.4569536423841, Blast_Score=525, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSD_VIBC3 (A5F579)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F579
- SMR:   A5F579
- STRING:   A5F579
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2137
- eggNOG:   COG0175
- HOGENOM:   HBG758022
- OMA:   GPEDEVK
- ProtClustDB:   PRK05253
- BioCyc:   VCHO345073:VC0395_A2137-MONOMER
- HAMAP:   MF_00064
- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF002936
- TIGRFAMs:   TIGR02039

Pfam domain/function: PF01507 PAPS_reduct

EC number: =2.7.7.4

Molecular weight: Translated: 35043; Mature: 35043

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQQRLTHLKQLEAESIHIIREVAAEFDNPVMMYSIGKDSSVMLHLTRKAFYPGKIPFPL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCEE
LHVDTDWKFRDMITFRDATAKKYGFDLIVHKNPEGLAAGINPFDHGSSKHTDIMKTQGLK
EEECCCCCEEHEEEECCCCHHHCCEEEEEEECCCCCEECCCCCCCCCCCCCHHHHHHHHH
QALNKYGFDAAFGGARRDEEKSRAKERVYSFRDKNHTWDPKNQRPELWRTYNGQINKGES
HHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEECCCCCCCCCE
IRVFPLSNWTELDIWQYIYLENIEIVPLYLADVRPVVQRDGMLIMVDDDRMKLREGEQIE
EEEEECCCCCCCHHEEEEEECCCEEEEEEECCCCHHHHCCCEEEEECCCCHHHCCCCCCC
HKSVRFRTLGCYPLTGAIESQANTLTEIIEEMLVATSSERQGRAIDHDQSGSMELKKRQG
CCCEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHCCC
YF
CC
>Mature Secondary Structure
MDQQRLTHLKQLEAESIHIIREVAAEFDNPVMMYSIGKDSSVMLHLTRKAFYPGKIPFPL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCEE
LHVDTDWKFRDMITFRDATAKKYGFDLIVHKNPEGLAAGINPFDHGSSKHTDIMKTQGLK
EEECCCCCEEHEEEECCCCHHHCCEEEEEEECCCCCEECCCCCCCCCCCCCHHHHHHHHH
QALNKYGFDAAFGGARRDEEKSRAKERVYSFRDKNHTWDPKNQRPELWRTYNGQINKGES
HHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEECCCCCCCCCE
IRVFPLSNWTELDIWQYIYLENIEIVPLYLADVRPVVQRDGMLIMVDDDRMKLREGEQIE
EEEEECCCCCCCHHEEEEEECCCEEEEEEECCCCHHHHCCCEEEEECCCCHHHCCCCCCC
HKSVRFRTLGCYPLTGAIESQANTLTEIIEEMLVATSSERQGRAIDHDQSGSMELKKRQG
CCCEEEEEECCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEHHHCCC
YF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA