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The map label for this gene is cysN

Identifier: 147673489

GI number: 147673489

Start: 2292998

End: 2294428

Strand: Reverse

Name: cysN

Synonym: VC0395_A2136

Alternate gene names: 147673489

Gene position: 2294428-2292998 (Counterclockwise)

Preceding gene: 147674260

Following gene: 147675279

Centisome position: 75.87

GC content: 48.99

Gene sequence:

>1431_bases
ATGAACAATGCAGTCAAAGAGCAGCTCGCTGAGCTCGGTATTGAAGGTTACCTCAATCAGCATCAACACAAATCGTTACT
GAGATTCTTAACTTGTGGCTCGGTCGATGATGGTAAAAGCACTCTGATCGGTCGTTTGCTCCACGATTCAAAGCAGATTT
ATGAAGATCAACTGGCCGCCGTCCATAACGATAGCCAGCGAGTTGGCACCACGGGCAGTCGCCCAGACTTAGCGCTGCTG
GTGGATGGCTTGCAAGCTGAGCGTGAGCAAGGCATCACGATTGATGTGGCGTATCGTTATTTCTCAACCCAGAAGCGCAA
ATTCATCATTGCCGATACACCGGGGCATGAGCAGTACACCCGTAATATGGCGACAGGCGCATCGACTTGTGATCTGGCGG
TGATCTTGATTGACGCGCGTAAAGGCGTGCTGGATCAAACGCGTCGTCACTCATTTATCTCCAACTTACTTGGCTTGAAA
CACTTTATTGTGGCGGTCAACAAAATGGATCTGGTCGATTATTCGCAAGATCGTTTTGAACAGATCCGCGCAGAATATCT
TGAGTTTTCAAAGCATTTACAGGGCGAGACCGAGATTCAGATCATCCCGCTTTCGGCATTGGAAGGCGATAACGTGGTCG
AAAAAAGCCGTTTGATGGATTGGTACCAAGGCCCATCACTGCTTGAGCTACTGGAAAATGTCGATATTGATCGCGACAAG
AGCAGTGGTGCGTTTCGCTTCCCAGTACAGTATGTGAATCGTCCTAACCTCGATTTTCGCGGCTTCGCGGGTACGATTGC
TTCTGGAGTGGTGAAAGTCGGCGATAAAATCAAAGCGCTACCATCGGGGAAAACCTCAACCGTGACAAGGATTGTGACTT
TTGATGGCGATTTGCCTCAAGCACAAGCCGGTTTGGCGGTCACTTTGACACTGGCTGATGAAATTGATATTAGCCGTGGT
GACTTGATTGTGTTGGAAAGTGCGCAAGTCGACAGCACTAATCATTTGTTGGCGGATGTGGTGTGGATGACAGAACAGCC
ACTGCAAGTGGGGCGTGATTACGATATTAAAATCGCGGGCAAGAAAACCGTCGGTCAAGTAAAAGCGGTGCGCCATCAGT
ACGACATCAATAACTTGTCGACCTACCATGCCGAGAGTTTACCGCTTAATGGGATTGGGTTGTGCGAGTGGACGTTTACC
CAAACTGTCGCGCTTGATAAATATCTGGATTGTGCCGATACCGGTGGTTTCATCATTATCGACCGCCTGACAAACGTGAC
CGTTGGCGCGGGTTTAGTGCGTGACAGTTTGCAAAATATCACTGGCCAAACCGAGTCGTTTTCAGCCTTTGAACTTGAGC
TCAATGCCTTGGTGCGTAAGCACTTCCCACATTGGCAAGCGATTGATTTAAGCCGCTTAGGTAAAGCGTGA

Upstream 100 bases:

>100_bases
CCTCCAGTGAGCGCCAAGGCCGCGCGATCGACCACGATCAGTCGGGATCAATGGAACTGAAAAAACGTCAAGGTTACTTC
TAAGGATCGAAGGAAAGCGT

Downstream 100 bases:

>100_bases
GTGAGGATGGATGAGTAAGCAAGCAGATAGCCTGCGCTTTGAACAAGATGCCAAGCTAGAGAATGTGGTGTGGCATCGTC
ATGCGGTGGATAAAGCGCAA

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MNNAVKEQLAELGIEGYLNQHQHKSLLRFLTCGSVDDGKSTLIGRLLHDSKQIYEDQLAAVHNDSQRVGTTGSRPDLALL
VDGLQAEREQGITIDVAYRYFSTQKRKFIIADTPGHEQYTRNMATGASTCDLAVILIDARKGVLDQTRRHSFISNLLGLK
HFIVAVNKMDLVDYSQDRFEQIRAEYLEFSKHLQGETEIQIIPLSALEGDNVVEKSRLMDWYQGPSLLELLENVDIDRDK
SSGAFRFPVQYVNRPNLDFRGFAGTIASGVVKVGDKIKALPSGKTSTVTRIVTFDGDLPQAQAGLAVTLTLADEIDISRG
DLIVLESAQVDSTNHLLADVVWMTEQPLQVGRDYDIKIAGKKTVGQVKAVRHQYDINNLSTYHAESLPLNGIGLCEWTFT
QTVALDKYLDCADTGGFIIIDRLTNVTVGAGLVRDSLQNITGQTESFSAFELELNALVRKHFPHWQAIDLSRLGKA

Sequences:

>Translated_476_residues
MNNAVKEQLAELGIEGYLNQHQHKSLLRFLTCGSVDDGKSTLIGRLLHDSKQIYEDQLAAVHNDSQRVGTTGSRPDLALL
VDGLQAEREQGITIDVAYRYFSTQKRKFIIADTPGHEQYTRNMATGASTCDLAVILIDARKGVLDQTRRHSFISNLLGLK
HFIVAVNKMDLVDYSQDRFEQIRAEYLEFSKHLQGETEIQIIPLSALEGDNVVEKSRLMDWYQGPSLLELLENVDIDRDK
SSGAFRFPVQYVNRPNLDFRGFAGTIASGVVKVGDKIKALPSGKTSTVTRIVTFDGDLPQAQAGLAVTLTLADEIDISRG
DLIVLESAQVDSTNHLLADVVWMTEQPLQVGRDYDIKIAGKKTVGQVKAVRHQYDINNLSTYHAESLPLNGIGLCEWTFT
QTVALDKYLDCADTGGFIIIDRLTNVTVGAGLVRDSLQNITGQTESFSAFELELNALVRKHFPHWQAIDLSRLGKA
>Mature_476_residues
MNNAVKEQLAELGIEGYLNQHQHKSLLRFLTCGSVDDGKSTLIGRLLHDSKQIYEDQLAAVHNDSQRVGTTGSRPDLALL
VDGLQAEREQGITIDVAYRYFSTQKRKFIIADTPGHEQYTRNMATGASTCDLAVILIDARKGVLDQTRRHSFISNLLGLK
HFIVAVNKMDLVDYSQDRFEQIRAEYLEFSKHLQGETEIQIIPLSALEGDNVVEKSRLMDWYQGPSLLELLENVDIDRDK
SSGAFRFPVQYVNRPNLDFRGFAGTIASGVVKVGDKIKALPSGKTSTVTRIVTFDGDLPQAQAGLAVTLTLADEIDISRG
DLIVLESAQVDSTNHLLADVVWMTEQPLQVGRDYDIKIAGKKTVGQVKAVRHQYDINNLSTYHAESLPLNGIGLCEWTFT
QTVALDKYLDCADTGGFIIIDRLTNVTVGAGLVRDSLQNITGQTESFSAFELELNALVRKHFPHWQAIDLSRLGKA

Specific function: May be the GTPase, regulating ATP sulfurylase activity

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily

Homologues:

Organism=Homo sapiens, GI223555963, Length=431, Percent_Identity=29.4663573085847, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI5729864, Length=431, Percent_Identity=29.4663573085847, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI4503471, Length=325, Percent_Identity=29.8461538461538, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI4503475, Length=457, Percent_Identity=26.0393873085339, Blast_Score=138, Evalue=9e-33,
Organism=Homo sapiens, GI194097354, Length=276, Percent_Identity=30.7971014492754, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI194018520, Length=276, Percent_Identity=30.7971014492754, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI194018522, Length=276, Percent_Identity=30.7971014492754, Blast_Score=136, Evalue=4e-32,
Organism=Homo sapiens, GI46094014, Length=276, Percent_Identity=30.4347826086957, Blast_Score=133, Evalue=4e-31,
Organism=Homo sapiens, GI34147630, Length=332, Percent_Identity=28.9156626506024, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1789108, Length=471, Percent_Identity=66.2420382165605, Blast_Score=647, Evalue=0.0,
Organism=Escherichia coli, GI1790412, Length=307, Percent_Identity=25.7328990228013, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789737, Length=307, Percent_Identity=25.7328990228013, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI2367247, Length=293, Percent_Identity=27.9863481228669, Blast_Score=75, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI115532067, Length=311, Percent_Identity=32.475884244373, Blast_Score=164, Evalue=8e-41,
Organism=Caenorhabditis elegans, GI115532065, Length=311, Percent_Identity=32.475884244373, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17552884, Length=439, Percent_Identity=27.5626423690205, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17569207, Length=439, Percent_Identity=27.5626423690205, Blast_Score=149, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI32566629, Length=310, Percent_Identity=30, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI32566303, Length=432, Percent_Identity=25.9259259259259, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI25141371, Length=230, Percent_Identity=30.8695652173913, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32566301, Length=138, Percent_Identity=35.5072463768116, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=346, Percent_Identity=28.3236994219653, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6322937, Length=395, Percent_Identity=29.3670886075949, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6325337, Length=323, Percent_Identity=29.4117647058824, Blast_Score=146, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6319594, Length=323, Percent_Identity=29.4117647058824, Blast_Score=146, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6320377, Length=336, Percent_Identity=28.5714285714286, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324761, Length=284, Percent_Identity=28.5211267605634, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI45550900, Length=431, Percent_Identity=26.4501160092807, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24652838, Length=305, Percent_Identity=30.4918032786885, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI17137572, Length=305, Percent_Identity=30.4918032786885, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI45553807, Length=275, Percent_Identity=32, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI45553816, Length=275, Percent_Identity=32, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24651721, Length=275, Percent_Identity=32, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI17864154, Length=275, Percent_Identity=32, Blast_Score=139, Evalue=4e-33,
Organism=Drosophila melanogaster, GI17137380, Length=326, Percent_Identity=28.2208588957055, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI281363316, Length=330, Percent_Identity=28.4848484848485, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI17864358, Length=330, Percent_Identity=28.4848484848485, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19921738, Length=276, Percent_Identity=30.4347826086957, Blast_Score=87, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSN_VIBC3 (A5F578)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F578
- SMR:   A5F578
- STRING:   A5F578
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2136
- eggNOG:   COG2895
- HOGENOM:   HBG307581
- OMA:   SAQIYED
- ProtClustDB:   PRK05124
- BioCyc:   VCHO345073:VC0395_A2136-MONOMER
- HAMAP:   MF_00062
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR02034
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: =2.7.7.4

Molecular weight: Translated: 52832; Mature: 52832

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNAVKEQLAELGIEGYLNQHQHKSLLRFLTCGSVDDGKSTLIGRLLHDSKQIYEDQLAA
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VHNDSQRVGTTGSRPDLALLVDGLQAEREQGITIDVAYRYFSTQKRKFIIADTPGHEQYT
HCCCCCCCCCCCCCCCCEEEECCHHHHHHCCCEEEEEEEECCCCCCEEEEEECCCCHHHH
RNMATGASTCDLAVILIDARKGVLDQTRRHSFISNLLGLKHFIVAVNKMDLVDYSQDRFE
HHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
QIRAEYLEFSKHLQGETEIQIIPLSALEGDNVVEKSRLMDWYQGPSLLELLENVDIDRDK
HHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
SSGAFRFPVQYVNRPNLDFRGFAGTIASGVVKVGDKIKALPSGKTSTVTRIVTFDGDLPQ
CCCEEECCHHHCCCCCCCCCCCHHHHHHHHHHHCCHHCCCCCCCCCEEEEEEEECCCCCC
AQAGLAVTLTLADEIDISRGDLIVLESAQVDSTNHLLADVVWMTEQPLQVGRDYDIKIAG
CCCCEEEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHCCCCCEEEEEC
KKTVGQVKAVRHQYDINNLSTYHAESLPLNGIGLCEWTFTQTVALDKYLDCADTGGFIII
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEE
DRLTNVTVGAGLVRDSLQNITGQTESFSAFELELNALVRKHFPHWQAIDLSRLGKA
EECCCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCCCCCEECHHHCCCC
>Mature Secondary Structure
MNNAVKEQLAELGIEGYLNQHQHKSLLRFLTCGSVDDGKSTLIGRLLHDSKQIYEDQLAA
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VHNDSQRVGTTGSRPDLALLVDGLQAEREQGITIDVAYRYFSTQKRKFIIADTPGHEQYT
HCCCCCCCCCCCCCCCCEEEECCHHHHHHCCCEEEEEEEECCCCCCEEEEEECCCCHHHH
RNMATGASTCDLAVILIDARKGVLDQTRRHSFISNLLGLKHFIVAVNKMDLVDYSQDRFE
HHHHCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
QIRAEYLEFSKHLQGETEIQIIPLSALEGDNVVEKSRLMDWYQGPSLLELLENVDIDRDK
HHHHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCC
SSGAFRFPVQYVNRPNLDFRGFAGTIASGVVKVGDKIKALPSGKTSTVTRIVTFDGDLPQ
CCCEEECCHHHCCCCCCCCCCCHHHHHHHHHHHCCHHCCCCCCCCCEEEEEEEECCCCCC
AQAGLAVTLTLADEIDISRGDLIVLESAQVDSTNHLLADVVWMTEQPLQVGRDYDIKIAG
CCCCEEEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCCHHHCCCCCEEEEEC
KKTVGQVKAVRHQYDINNLSTYHAESLPLNGIGLCEWTFTQTVALDKYLDCADTGGFIII
CCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEE
DRLTNVTVGAGLVRDSLQNITGQTESFSAFELELNALVRKHFPHWQAIDLSRLGKA
EECCCCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCCCCCEECHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA