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The map label for this gene is hemF

Identifier: 147674221

GI number: 147674221

Start: 2617722

End: 2618639

Strand: Reverse

Name: hemF

Synonym: VC0395_A2465

Alternate gene names: 147674221

Gene position: 2618639-2617722 (Counterclockwise)

Preceding gene: 147673407

Following gene: 147674570

Centisome position: 86.59

GC content: 49.89

Gene sequence:

>918_bases
GTGGAGTCGATAGTCGATAAACAAGCAGTGAAGCACTTTTTACTGCAGCTACAAGATAAGATTTGCCAACAATTGGAAGC
CACAGATGGCCAAGCGCAATTTATTGAAGATGCGTGGCAGCGTGAACCGGGTGAGAAACTGGGTGGTGGTGGCCGTACGC
GAGTCATGCGTGAGGGCGCTGTTTTCGAACAGGGTGGCGTGAACTTTTCCCATGTATTTGGTGAGCAGATGCCTGCCTCT
GCGACGGCCCATCGTCCAGAATTGGCAGGTCGCCGCTTTGAAGCCATGGGCGTTTCTCTGGTCATGCACCCGAAAAATCC
GTATGTTCCGACCTCTCATGCCAATGTGCGCTTTTTTATTGCTGAAAAAGAGGGCGAAGCTCCGATCTGGTGGTTTGGTG
GTGGTTTTGACCTTACGCCGTTTTATCCCTTTGTTGAGGATGGCCAGCACTGGCATCAAACTGCTAAAAATATCTGCGCC
CCTTTTGGGTCAGAAATCTATAACGAACATAAAGCATGGTGTGATCGCTATTTCTACTTACCACACCGCAATGAAACACG
CGGCATCGGCGGATTGTTTTTTGATGATCTCAATGAATGGTCATTCGAGCAATGTTTCGCTTATATGCAGGCGGTCGGCG
AAGGTTATACCCAAGCTTATGTGCCGATTGTCGAAAAACGCAAAAACACGCCCTTTACAGAGCGCGAGCGCCAATTTCAA
CTCTATCGTCGCGGTCGATACGTTGAATTTAACTTGGTGTTGGATCGCGGCACGCTGTTTGGCCTTCAAACCGGTGGGCG
TACCGAATCAATCTTGATGTCGATGCCTCCCTTAGCGCGTTGGGAATACGCATATCAACCGCAAGCAGGAACGCCAGAAG
CCAAGCTAAGCGAGTTTTTAGTGCCTCGTGAATGGTGA

Upstream 100 bases:

>100_bases
AGTAGAAGGGAAGACCAGTGGCCGTCATGGACCGAGCGAAATTCGTGATGCTCGCAGCTTACAAGTGTTAAGACAGGGAT
AACCCTGCGCAGGAGAGAAT

Downstream 100 bases:

>100_bases
CAGGCAAGGTTCTGAGTGATAGGGTCGCGCAAGTGACCCTTTTGATTGTTTAGGCAAGGATATGGCTTCTCAAATCGATC
AGTATGCCGTTTTTGGTAAC

Product: coproporphyrinogen III oxidase

Products: NA

Alternate protein names: Coprogen oxidase; Coproporphyrinogenase

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MESIVDKQAVKHFLLQLQDKICQQLEATDGQAQFIEDAWQREPGEKLGGGGRTRVMREGAVFEQGGVNFSHVFGEQMPAS
ATAHRPELAGRRFEAMGVSLVMHPKNPYVPTSHANVRFFIAEKEGEAPIWWFGGGFDLTPFYPFVEDGQHWHQTAKNICA
PFGSEIYNEHKAWCDRYFYLPHRNETRGIGGLFFDDLNEWSFEQCFAYMQAVGEGYTQAYVPIVEKRKNTPFTERERQFQ
LYRRGRYVEFNLVLDRGTLFGLQTGGRTESILMSMPPLARWEYAYQPQAGTPEAKLSEFLVPREW

Sequences:

>Translated_305_residues
MESIVDKQAVKHFLLQLQDKICQQLEATDGQAQFIEDAWQREPGEKLGGGGRTRVMREGAVFEQGGVNFSHVFGEQMPAS
ATAHRPELAGRRFEAMGVSLVMHPKNPYVPTSHANVRFFIAEKEGEAPIWWFGGGFDLTPFYPFVEDGQHWHQTAKNICA
PFGSEIYNEHKAWCDRYFYLPHRNETRGIGGLFFDDLNEWSFEQCFAYMQAVGEGYTQAYVPIVEKRKNTPFTERERQFQ
LYRRGRYVEFNLVLDRGTLFGLQTGGRTESILMSMPPLARWEYAYQPQAGTPEAKLSEFLVPREW
>Mature_305_residues
MESIVDKQAVKHFLLQLQDKICQQLEATDGQAQFIEDAWQREPGEKLGGGGRTRVMREGAVFEQGGVNFSHVFGEQMPAS
ATAHRPELAGRRFEAMGVSLVMHPKNPYVPTSHANVRFFIAEKEGEAPIWWFGGGFDLTPFYPFVEDGQHWHQTAKNICA
PFGSEIYNEHKAWCDRYFYLPHRNETRGIGGLFFDDLNEWSFEQCFAYMQAVGEGYTQAYVPIVEKRKNTPFTERERQFQ
LYRRGRYVEFNLVLDRGTLFGLQTGGRTESILMSMPPLARWEYAYQPQAGTPEAKLSEFLVPREW

Specific function: Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III

COG id: COG0408

COG function: function code H; Coproporphyrinogen III oxidase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aerobic coproporphyrinogen-III oxidase family

Homologues:

Organism=Homo sapiens, GI41393599, Length=308, Percent_Identity=45.4545454545455, Blast_Score=265, Evalue=3e-71,
Organism=Escherichia coli, GI1788777, Length=300, Percent_Identity=74.3333333333333, Blast_Score=468, Evalue=1e-133,
Organism=Saccharomyces cerevisiae, GI6320249, Length=318, Percent_Identity=43.3962264150943, Blast_Score=259, Evalue=4e-70,
Organism=Drosophila melanogaster, GI17864388, Length=313, Percent_Identity=45.6869009584665, Blast_Score=265, Evalue=4e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM6_VIBC3 (A5F4C4)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F4C4
- STRING:   A5F4C4
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2465
- eggNOG:   COG0408
- HOGENOM:   HBG631180
- OMA:   HMNTRFV
- ProtClustDB:   PRK05330
- BioCyc:   VCHO345073:VC0395_A2465-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00333
- InterPro:   IPR001260
- InterPro:   IPR018375
- Gene3D:   G3DSA:3.40.1500.10
- PANTHER:   PTHR10755
- PIRSF:   PIRSF000166
- PRINTS:   PR00073

Pfam domain/function: PF01218 Coprogen_oxidas; SSF102886 Coprogen_oxidas

EC number: =1.3.3.3

Molecular weight: Translated: 35126; Mature: 35126

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS01021 COPROGEN_OXIDASE

Important sites: ACT_SITE 113-113 BINDING 99-99

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESIVDKQAVKHFLLQLQDKICQQLEATDGQAQFIEDAWQREPGEKLGGGGRTRVMREGA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHCCCCHHHEEECCC
VFEQGGVNFSHVFGEQMPASATAHRPELAGRRFEAMGVSLVMHPKNPYVPTSHANVRFFI
EECCCCCCHHHHHCCCCCCCCCCCCHHHHCCEEHCCCEEEEECCCCCCCCCCCCCEEEEE
AEKEGEAPIWWFGGGFDLTPFYPFVEDGQHWHQTAKNICAPFGSEIYNEHKAWCDRYFYL
EECCCCCCEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEC
PHRNETRGIGGLFFDDLNEWSFEQCFAYMQAVGEGYTQAYVPIVEKRKNTPFTERERQFQ
CCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHH
LYRRGRYVEFNLVLDRGTLFGLQTGGRTESILMSMPPLARWEYAYQPQAGTPEAKLSEFL
HHHCCCEEEEEEEEECCEEEEECCCCCCHHHEECCCCCHHCCEEECCCCCCCHHHHHHHC
VPREW
CCCCC
>Mature Secondary Structure
MESIVDKQAVKHFLLQLQDKICQQLEATDGQAQFIEDAWQREPGEKLGGGGRTRVMREGA
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHCCCCHHHEEECCC
VFEQGGVNFSHVFGEQMPASATAHRPELAGRRFEAMGVSLVMHPKNPYVPTSHANVRFFI
EECCCCCCHHHHHCCCCCCCCCCCCHHHHCCEEHCCCEEEEECCCCCCCCCCCCCEEEEE
AEKEGEAPIWWFGGGFDLTPFYPFVEDGQHWHQTAKNICAPFGSEIYNEHKAWCDRYFYL
EECCCCCCEEEECCCCCCCCCCCCHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEC
PHRNETRGIGGLFFDDLNEWSFEQCFAYMQAVGEGYTQAYVPIVEKRKNTPFTERERQFQ
CCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHH
LYRRGRYVEFNLVLDRGTLFGLQTGGRTESILMSMPPLARWEYAYQPQAGTPEAKLSEFL
HHHCCCEEEEEEEEECCEEEEECCCCCCHHHEECCCCCHHCCEEECCCCCCCHHHHHHHC
VPREW
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA