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The map label for this gene is rimN

Identifier: 147673407

GI number: 147673407

Start: 2618658

End: 2619224

Strand: Reverse

Name: rimN

Synonym: VC0395_A2466

Alternate gene names: 147673407

Gene position: 2619224-2618658 (Counterclockwise)

Preceding gene: 147673250

Following gene: 147674221

Centisome position: 86.61

GC content: 53.62

Gene sequence:

>567_bases
GTGGCTCTGGTGGAGAATCTACAACAGGCGGTGGATGCGCTGCGCAAAGGTTGCGTTATTGCCTATCCAACCGAAGGGGT
GTTTGGTTTGGGGTGTGATCCGGATAACCAAACCGCTATGCTGCGCTTATTGGCGATCAAGCAGCGCCCCGTGGAAAAAG
GGGTGATCTTAATTGCCGCTAGCTATGCGCAGTTGCGCCCTTATGTCGATGAAACGCAATTAACCGCAGAGCAACTGACA
CAAGTGCTGGCTTCATGGCCCGCGCCACTGACTTGGGTCATGCCAGCCAGCGGTGATACGCCAAGCTGGGTTCGTGGTCA
GTTTGATACCGTTGCCGTGCGTGTTTCGGATCATCCGGTCGTTCAGAAGTTATGTCTCGCCTTTGGGAAGCCACTGACGT
CAACCAGTGCTAATCTAAGTGGCCAGCCCGCGTGCGTAACTCAACAAGAAGTCATGGTTCAGCTAGGCAATCAGATTGCT
GTGGTAGTAGAAGGGAAGACCAGTGGCCGTCATGGACCGAGCGAAATTCGTGATGCTCGCAGCTTACAAGTGTTAAGACA
GGGATAA

Upstream 100 bases:

>100_bases
CGGGGATTTTTTTCTCAAGCAAACGTTTGCTTTTTCTGGTTGATGAGAAGAACTGGTCAATTGCTCCACGATTTGTACAC
TGAGCACAAATGAAAGAGGA

Downstream 100 bases:

>100_bases
CCCTGCGCAGGAGAGAATGTGGAGTCGATAGTCGATAAACAAGCAGTGAAGCACTTTTTACTGCAGCTACAAGATAAGAT
TTGCCAACAATTGGAAGCCA

Product: Sua5/YciO/YrdC family protein

Products: NA

Alternate protein names: t(6)A37 threonylcarbamoyladenosine biosynthesis protein RimN

Number of amino acids: Translated: 188; Mature: 187

Protein sequence:

>188_residues
MALVENLQQAVDALRKGCVIAYPTEGVFGLGCDPDNQTAMLRLLAIKQRPVEKGVILIAASYAQLRPYVDETQLTAEQLT
QVLASWPAPLTWVMPASGDTPSWVRGQFDTVAVRVSDHPVVQKLCLAFGKPLTSTSANLSGQPACVTQQEVMVQLGNQIA
VVVEGKTSGRHGPSEIRDARSLQVLRQG

Sequences:

>Translated_188_residues
MALVENLQQAVDALRKGCVIAYPTEGVFGLGCDPDNQTAMLRLLAIKQRPVEKGVILIAASYAQLRPYVDETQLTAEQLT
QVLASWPAPLTWVMPASGDTPSWVRGQFDTVAVRVSDHPVVQKLCLAFGKPLTSTSANLSGQPACVTQQEVMVQLGNQIA
VVVEGKTSGRHGPSEIRDARSLQVLRQG
>Mature_187_residues
ALVENLQQAVDALRKGCVIAYPTEGVFGLGCDPDNQTAMLRLLAIKQRPVEKGVILIAASYAQLRPYVDETQLTAEQLTQ
VLASWPAPLTWVMPASGDTPSWVRGQFDTVAVRVSDHPVVQKLCLAFGKPLTSTSANLSGQPACVTQQEVMVQLGNQIAV
VVEGKTSGRHGPSEIRDARSLQVLRQG

Specific function: Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine

COG id: COG0009

COG function: function code J; Putative translation factor (SUA5)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain

Homologues:

Organism=Escherichia coli, GI2367210, Length=189, Percent_Identity=56.6137566137566, Blast_Score=219, Evalue=8e-59,
Organism=Escherichia coli, GI87081864, Length=168, Percent_Identity=27.9761904761905, Blast_Score=75, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321269, Length=160, Percent_Identity=28.125, Blast_Score=65, Evalue=6e-12,
Organism=Drosophila melanogaster, GI78707274, Length=173, Percent_Identity=33.5260115606936, Blast_Score=74, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMN_VIBC3 (A5F4C5)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F4C5
- STRING:   A5F4C5
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A2466
- eggNOG:   COG0009
- HOGENOM:   HBG738264
- OMA:   FGLGCNP
- ProtClustDB:   CLSK793439
- BioCyc:   VCHO345073:VC0395_A2466-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01852
- InterPro:   IPR017945
- InterPro:   IPR004388
- InterPro:   IPR006070
- Gene3D:   G3DSA:3.90.870.10
- TIGRFAMs:   TIGR00057

Pfam domain/function: PF01300 Sua5_yciO_yrdC; SSF55821 DHBP_synth_RibB-like_a/b_dom

EC number: NA

Molecular weight: Translated: 20248; Mature: 20116

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVENLQQAVDALRKGCVIAYPTEGVFGLGCDPDNQTAMLRLLAIKQRPVEKGVILIAA
CHHHHHHHHHHHHHHCCCEEEECCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEE
SYAQLRPYVDETQLTAEQLTQVLASWPAPLTWVMPASGDTPSWVRGQFDTVAVRVSDHPV
CHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHCCCCEEEEEEECCCHH
VQKLCLAFGKPLTSTSANLSGQPACVTQQEVMVQLGNQIAVVVEGKTSGRHGPSEIRDAR
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHH
SLQVLRQG
HHHHHHCC
>Mature Secondary Structure 
ALVENLQQAVDALRKGCVIAYPTEGVFGLGCDPDNQTAMLRLLAIKQRPVEKGVILIAA
HHHHHHHHHHHHHHCCCEEEECCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEE
SYAQLRPYVDETQLTAEQLTQVLASWPAPLTWVMPASGDTPSWVRGQFDTVAVRVSDHPV
CHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHCCCCEEEEEEECCCHH
VQKLCLAFGKPLTSTSANLSGQPACVTQQEVMVQLGNQIAVVVEGKTSGRHGPSEIRDAR
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHH
SLQVLRQG
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA