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The map label for this gene is parE [H]

Identifier: 147674220

GI number: 147674220

Start: 2158781

End: 2160661

Strand: Reverse

Name: parE [H]

Synonym: VC0395_A2008

Alternate gene names: 147674220

Gene position: 2160661-2158781 (Counterclockwise)

Preceding gene: 147673053

Following gene: 147674657

Centisome position: 71.45

GC content: 52.63

Gene sequence:

>1881_bases
ATGACTGAACAATATAATGCTGGGGCCATTGAGGTTCTCAATGGCTTAGAACCAGTGCGTCGCAGACCGGGAATGTATAC
CGACACCACGCGCCCCAATCACCTTGGGCAAGAAGTGATCGACAACTCCGTCGATGAAGCGCTGGCCGGATACGCCTCCA
AAATTCAGGTAATTCTTCATGCCGATCAATCGCTCGAAGTGATTGATGACGGGCGAGGCATGCCCGTCGATATTCACCCC
GAAGAGAAGGTCTCAGGGGTGGAGCTTATCCTGTGCAAACTGCACGCCGGTGGTAAGTTTTCCAACAAAAACTACCAATT
TTCCGGTGGCTTGCATGGGGTGGGTATTTCGGTGGTGAATGCGCTCTCCAAACGGGTAGAAGTGGCGGTTCGCCGTGATG
GTCAAGTGTATGAAATTGCCTTTGAACATGGCGATAAAGTGACTGAGCTGACGGTTACGGGGACTTGCGGCCGTCGCAAT
ACCGGTACTAGCGTCCATTTCTGGCCAGACACCAAATATTTTGACTCACCGAATTTCTCCGTATCGCGCCTGATTAATAA
CTTGCGTGCCAAGGCGGTACTTTGCCCCGGTTTAGAAATCATCTTTACCGATAAGGTCAATAACAACGAGCACCGCTGGT
TGTACGAAGATGGTCTAAAAGATTATCTGGCCGAAGGGGTGAAAGGTTATACCTTACTGCCGGAAGAGCCGTTTACCGGC
GAATTTACTGCGCAAACCGAAGCGGCAACGTGGGCGGTGATTTGGCTACCCGAAGGTGGCGAGCTCATCACCGAAAGCTA
CGTTAACCTGATCCCAACTGCGCAAGGTGGTACCCATGTCAACGGTTTACGCCAAGGCTTGCTGGATGCGATGCGCGAGT
TTTGTGAGTTCCGCAATCTGCTGCCACGCGGGGTAAAATTGACAGGCGAGGACGTGTTTGACCGCTGTGCTTATGTGCTG
TCGATCAAGATGCAAGATCCGCAGTTTGCTGGCCAAACCAAAGAGCGCCTATCTTCACGCCAATCGGCGGCGTTTGTCTC
CGGTGTGGTGAAAGATGCCTTTAGCTTGTGGCTTAACGAAAAACCACAACTGGCAGAGCAGTTAGCCGAAGTTTGTATTG
CTAATGCGCACAGCCGAATGCGTGCCAGCAAGAAAGTGGTACGTAAGAAGATCGCTTCAGGCCCTGCCTTACCGGGTAAA
TTAACCGACTGTACAGTGCAAGACCTTAACCGCACCGAACTCTTCCTGGTGGAAGGGGATTCGGCGGGCGGCTCGGCGAA
GCAAGCGCGGGATCGTGAATTCCAAGCGGTGATGCCACTGCGCGGCAAAATCCTCAATACATGGGAAGTTTCTGCCGATC
AAGTATTGGCTTCACAAGAAGTACACGACATCTCAGTTGCATTGGGGATTGACCCTGACAGCGATAACCTCGAAGCACTG
CGTTACGGCAAGGTCTGTATCCTTGCCGATGCGGACTCGGATGGTCTGCACATTGCGACCTTGCTTTGCGCCCTATTTAC
CCGCCATTTCCGCGCGCTGGTGAAAGCGGGTCACGTCTATGTTGCCATGCCGCCGCTCTACCGCATCGACTGTGGTAAAG
AGGTGTTCTACGCACTCGATGATCAAGAAAAAGAGGGCGTGTTAGAGCGTCTGAGCCAGAAAAAAGCCAAAGTGAACGTG
CAACGCTTTAAAGGCTTGGGTGAAATGAACCCGCTGCAATTGCGTGAAACCACCATGGATCCCAACACTCGCCGCTTGGT
GCAACTGACCATTGATGATGCTGAAGCGACCGACGAGATGATGGATATGCTGCTGGGTAAAAAGCGTGCGGATGACCGCC
GAGCTTGGCTGCAACGTAACGGCGATATGGCAGAGGTGTAA

Upstream 100 bases:

>100_bases
AAATAATGCGATTTTTCGCACAATTTATGCACAAAAAATCAGGTTTTCACACGGCGCTGATAAAGTTACCAATCATTGAG
CAAATAAAGCATTCCGTATT

Downstream 100 bases:

>100_bases
CGAATGTCTACTGAAATCAGCTTTGATGGCGTTGAACAACTGCCGCTGCGCAAATTTACTGAAGACGCTTATCTCAACTA
TTCGATGTACGTCATCATGG

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 626; Mature: 625

Protein sequence:

>626_residues
MTEQYNAGAIEVLNGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGYASKIQVILHADQSLEVIDDGRGMPVDIHP
EEKVSGVELILCKLHAGGKFSNKNYQFSGGLHGVGISVVNALSKRVEVAVRRDGQVYEIAFEHGDKVTELTVTGTCGRRN
TGTSVHFWPDTKYFDSPNFSVSRLINNLRAKAVLCPGLEIIFTDKVNNNEHRWLYEDGLKDYLAEGVKGYTLLPEEPFTG
EFTAQTEAATWAVIWLPEGGELITESYVNLIPTAQGGTHVNGLRQGLLDAMREFCEFRNLLPRGVKLTGEDVFDRCAYVL
SIKMQDPQFAGQTKERLSSRQSAAFVSGVVKDAFSLWLNEKPQLAEQLAEVCIANAHSRMRASKKVVRKKIASGPALPGK
LTDCTVQDLNRTELFLVEGDSAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHDISVALGIDPDSDNLEAL
RYGKVCILADADSDGLHIATLLCALFTRHFRALVKAGHVYVAMPPLYRIDCGKEVFYALDDQEKEGVLERLSQKKAKVNV
QRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDAEATDEMMDMLLGKKRADDRRAWLQRNGDMAEV

Sequences:

>Translated_626_residues
MTEQYNAGAIEVLNGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGYASKIQVILHADQSLEVIDDGRGMPVDIHP
EEKVSGVELILCKLHAGGKFSNKNYQFSGGLHGVGISVVNALSKRVEVAVRRDGQVYEIAFEHGDKVTELTVTGTCGRRN
TGTSVHFWPDTKYFDSPNFSVSRLINNLRAKAVLCPGLEIIFTDKVNNNEHRWLYEDGLKDYLAEGVKGYTLLPEEPFTG
EFTAQTEAATWAVIWLPEGGELITESYVNLIPTAQGGTHVNGLRQGLLDAMREFCEFRNLLPRGVKLTGEDVFDRCAYVL
SIKMQDPQFAGQTKERLSSRQSAAFVSGVVKDAFSLWLNEKPQLAEQLAEVCIANAHSRMRASKKVVRKKIASGPALPGK
LTDCTVQDLNRTELFLVEGDSAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHDISVALGIDPDSDNLEAL
RYGKVCILADADSDGLHIATLLCALFTRHFRALVKAGHVYVAMPPLYRIDCGKEVFYALDDQEKEGVLERLSQKKAKVNV
QRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDAEATDEMMDMLLGKKRADDRRAWLQRNGDMAEV
>Mature_625_residues
TEQYNAGAIEVLNGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGYASKIQVILHADQSLEVIDDGRGMPVDIHPE
EKVSGVELILCKLHAGGKFSNKNYQFSGGLHGVGISVVNALSKRVEVAVRRDGQVYEIAFEHGDKVTELTVTGTCGRRNT
GTSVHFWPDTKYFDSPNFSVSRLINNLRAKAVLCPGLEIIFTDKVNNNEHRWLYEDGLKDYLAEGVKGYTLLPEEPFTGE
FTAQTEAATWAVIWLPEGGELITESYVNLIPTAQGGTHVNGLRQGLLDAMREFCEFRNLLPRGVKLTGEDVFDRCAYVLS
IKMQDPQFAGQTKERLSSRQSAAFVSGVVKDAFSLWLNEKPQLAEQLAEVCIANAHSRMRASKKVVRKKIASGPALPGKL
TDCTVQDLNRTELFLVEGDSAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHDISVALGIDPDSDNLEALR
YGKVCILADADSDGLHIATLLCALFTRHFRALVKAGHVYVAMPPLYRIDCGKEVFYALDDQEKEGVLERLSQKKAKVNVQ
RFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDAEATDEMMDMLLGKKRADDRRAWLQRNGDMAEV

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=630, Percent_Identity=24.6031746031746, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI19913408, Length=591, Percent_Identity=25.7191201353638, Blast_Score=133, Evalue=6e-31,
Organism=Escherichia coli, GI1789408, Length=628, Percent_Identity=80.7324840764331, Blast_Score=1074, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=555, Percent_Identity=40.7207207207207, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI212645845, Length=600, Percent_Identity=24, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17535065, Length=627, Percent_Identity=23.1259968102073, Blast_Score=112, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI212645657, Length=253, Percent_Identity=27.6679841897233, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=632, Percent_Identity=25.9493670886076, Blast_Score=142, Evalue=1e-34,
Organism=Drosophila melanogaster, GI17136538, Length=637, Percent_Identity=23.7048665620094, Blast_Score=127, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 69467; Mature: 69336

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQYNAGAIEVLNGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGYASKIQVILH
CCCCCCCCHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHEEEEEEE
ADQSLEVIDDGRGMPVDIHPEEKVSGVELILCKLHAGGKFSNKNYQFSGGLHGVGISVVN
CCCCCEEEECCCCCCEECCCCCCCCCEEEEEEEEECCCCCCCCCEEECCCCCHHHHHHHH
ALSKRVEVAVRRDGQVYEIAFEHGDKVTELTVTGTCGRRNTGTSVHFWPDTKYFDSPNFS
HHHHHHEEEEECCCCEEEEEECCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCH
VSRLINNLRAKAVLCPGLEIIFTDKVNNNEHRWLYEDGLKDYLAEGVKGYTLLPEEPFTG
HHHHHHHHHHCEEECCCEEEEEEECCCCCCCCEEHHHHHHHHHHCCCCCCEECCCCCCCC
EFTAQTEAATWAVIWLPEGGELITESYVNLIPTAQGGTHVNGLRQGLLDAMREFCEFRNL
CEEECCCCCEEEEEEECCCCCHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHC
LPRGVKLTGEDVFDRCAYVLSIKMQDPQFAGQTKERLSSRQSAAFVSGVVKDAFSLWLNE
CCCCCEECCHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KPQLAEQLAEVCIANAHSRMRASKKVVRKKIASGPALPGKLTDCTVQDLNRTELFLVEGD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECC
SAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHDISVALGIDPDSDNLEAL
CCCCCHHHHHCCCHHEECCCCCCEEEEECCCHHHHHHHCCCEEEEEEEECCCCCCCCHHE
RYGKVCILADADSDGLHIATLLCALFTRHFRALVKAGHVYVAMPPLYRIDCGKEVFYALD
EECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCCEEEEEEC
DQEKEGVLERLSQKKAKVNVQRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDAEATDEM
CCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEECCCCCCCEEEEEEEECCHHHHHHH
MDMLLGKKRADDRRAWLQRNGDMAEV
HHHHHCCCCCHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TEQYNAGAIEVLNGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGYASKIQVILH
CCCCCCCHHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHEEEEEEE
ADQSLEVIDDGRGMPVDIHPEEKVSGVELILCKLHAGGKFSNKNYQFSGGLHGVGISVVN
CCCCCEEEECCCCCCEECCCCCCCCCEEEEEEEEECCCCCCCCCEEECCCCCHHHHHHHH
ALSKRVEVAVRRDGQVYEIAFEHGDKVTELTVTGTCGRRNTGTSVHFWPDTKYFDSPNFS
HHHHHHEEEEECCCCEEEEEECCCCCEEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCH
VSRLINNLRAKAVLCPGLEIIFTDKVNNNEHRWLYEDGLKDYLAEGVKGYTLLPEEPFTG
HHHHHHHHHHCEEECCCEEEEEEECCCCCCCCEEHHHHHHHHHHCCCCCCEECCCCCCCC
EFTAQTEAATWAVIWLPEGGELITESYVNLIPTAQGGTHVNGLRQGLLDAMREFCEFRNL
CEEECCCCCEEEEEEECCCCCHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHC
LPRGVKLTGEDVFDRCAYVLSIKMQDPQFAGQTKERLSSRQSAAFVSGVVKDAFSLWLNE
CCCCCEECCHHHHHHHEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KPQLAEQLAEVCIANAHSRMRASKKVVRKKIASGPALPGKLTDCTVQDLNRTELFLVEGD
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEECC
SAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHDISVALGIDPDSDNLEAL
CCCCCHHHHHCCCHHEECCCCCCEEEEECCCHHHHHHHCCCEEEEEEEECCCCCCCCHHE
RYGKVCILADADSDGLHIATLLCALFTRHFRALVKAGHVYVAMPPLYRIDCGKEVFYALD
EECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCCEEEEEEC
DQEKEGVLERLSQKKAKVNVQRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDAEATDEM
CCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCEEEECCCCCCCEEEEEEEECCHHHHHHH
MDMLLGKKRADDRRAWLQRNGDMAEV
HHHHHCCCCCHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2170028; 8388096; 9278503; 8227000; 8980775 [H]