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The map label for this gene is lacA [C]

Identifier: 147674213

GI number: 147674213

Start: 2769041

End: 2769607

Strand: Reverse

Name: lacA [C]

Synonym: VC0395_A2618

Alternate gene names: 147674213

Gene position: 2769607-2769041 (Counterclockwise)

Preceding gene: 147675347

Following gene: 147673869

Centisome position: 91.59

GC content: 37.04

Gene sequence:

>567_bases
TTGCAGATGGCTGGCTTTGGTTTAATTGCAAAATTACGTAATAAAATTCGGGTAAAACCTAACAACAAGATTCAAATAGG
TAAAAATACTCGTATCCGCTATTGCGACATTTATGTTAATGGTAATAATAACCAACTGATTATCCATAATGGTGCAAATT
TAAAAGGCGTCTCTATCGAGCTCAATGGCAACAATTGCCTCTTAGAAATTGGCGAAAATTGCGTGATTGGCGAAAATTGT
TTTTTATCATGCCGAGAATCCGGAACAAAATTAGTGATTGGTAAGGAATGTATGTTTTCGCGCAATGTAAAACTAATGAC
CAGTGATGGACATGATATTTTACGTGATGGAAAAAGGATTAATCCGGCTAAAAGTATTTATATAGGTTCTCATGTATGGC
TTGCGGATAACGTCACTATTTTAAAAGGAGTGGATATCGGTTCTGGTTCGATTATTGGCATCAATTCTACGGTAACAAAA
TCGATTGGCCAGTATTGCATTGCCGCGGGCAACCCAGCGAGAAAAATCAAAGACAATATTAGTTGGAACACGACCCTGAG
CTATTAA

Upstream 100 bases:

>100_bases
CGTCGGATTGCGATTTTTCCAAGAACCGACACTTTTGGTCTGTGGCGAAAAGTGAAAGGGAATTATCTATTTAGAAAAAT
GAACAAAAAATAATAAGAGG

Downstream 100 bases:

>100_bases
AATGAATAATAAAATAACTAAAACCTCAATTTTCCTTACTATCTCACTACTTTTAATTACACCGGGATTTTCTGTTGTAG
CGGTAGGATTACTGACTCTC

Product: hexapaptide repeat-containing transferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MQMAGFGLIAKLRNKIRVKPNNKIQIGKNTRIRYCDIYVNGNNNQLIIHNGANLKGVSIELNGNNCLLEIGENCVIGENC
FLSCRESGTKLVIGKECMFSRNVKLMTSDGHDILRDGKRINPAKSIYIGSHVWLADNVTILKGVDIGSGSIIGINSTVTK
SIGQYCIAAGNPARKIKDNISWNTTLSY

Sequences:

>Translated_188_residues
MQMAGFGLIAKLRNKIRVKPNNKIQIGKNTRIRYCDIYVNGNNNQLIIHNGANLKGVSIELNGNNCLLEIGENCVIGENC
FLSCRESGTKLVIGKECMFSRNVKLMTSDGHDILRDGKRINPAKSIYIGSHVWLADNVTILKGVDIGSGSIIGINSTVTK
SIGQYCIAAGNPARKIKDNISWNTTLSY
>Mature_188_residues
MQMAGFGLIAKLRNKIRVKPNNKIQIGKNTRIRYCDIYVNGNNNQLIIHNGANLKGVSIELNGNNCLLEIGENCVIGENC
FLSCRESGTKLVIGKECMFSRNVKLMTSDGHDILRDGKRINPAKSIYIGSHVWLADNVTILKGVDIGSGSIIGINSTVTK
SIGQYCIAAGNPARKIKDNISWNTTLSY

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6322243, Length=114, Percent_Identity=34.2105263157895, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 20584; Mature: 20584

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMAGFGLIAKLRNKIRVKPNNKIQIGKNTRIRYCDIYVNGNNNQLIIHNGANLKGVSIE
CCCCCHHHHHHHCCEEEECCCCEEEECCCCEEEEEEEEEECCCCEEEEECCCCEEEEEEE
LNGNNCLLEIGENCVIGENCFLSCRESGTKLVIGKECMFSRNVKLMTSDGHDILRDGKRI
ECCCEEEEEECCCEEECCCEEEEECCCCCEEEEECCCEEECCEEEEECCCHHHHHCCCCC
NPAKSIYIGSHVWLADNVTILKGVDIGSGSIIGINSTVTKSIGQYCIAAGNPARKIKDNI
CCCCEEEEECEEEEECCEEEEEEEECCCCCEEEECHHHHHHHCCEEEECCCCHHHHHCCC
SWNTTLSY
CEEEEECC
>Mature Secondary Structure
MQMAGFGLIAKLRNKIRVKPNNKIQIGKNTRIRYCDIYVNGNNNQLIIHNGANLKGVSIE
CCCCCHHHHHHHCCEEEECCCCEEEECCCCEEEEEEEEEECCCCEEEEECCCCEEEEEEE
LNGNNCLLEIGENCVIGENCFLSCRESGTKLVIGKECMFSRNVKLMTSDGHDILRDGKRI
ECCCEEEEEECCCEEECCCEEEEECCCCCEEEEECCCEEECCEEEEECCCHHHHHCCCCC
NPAKSIYIGSHVWLADNVTILKGVDIGSGSIIGINSTVTKSIGQYCIAAGNPARKIKDNI
CCCCEEEEECEEEEECCEEEEEEEECCCCCEEEECHHHHHHHCCEEEECCCCHHHHHCCC
SWNTTLSY
CEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]