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The map label for this gene is gspD

Identifier: 147674158

GI number: 147674158

Start: 2459042

End: 2461066

Strand: Reverse

Name: gspD

Synonym: VC0395_A2306

Alternate gene names: 147674158

Gene position: 2461066-2459042 (Counterclockwise)

Preceding gene: 147675412

Following gene: 147673384

Centisome position: 81.38

GC content: 49.73

Gene sequence:

>2025_bases
GTGAAATATTGGCTGAAAAAAAGTTCATGGCTGTTGGCGGGCAGTTTGCTGTCTACCCCTCTAGCGATGGCAAACGAGTT
TAGCGCCAGCTTTAAAGGCACCGACATTCAAGAATTTATCAATATTGTGGGACGCAATCTTGAGAAAACCATCATTGTCG
ACCCTTCGGTACGCGGCAAAGTCGATGTGCGCAGTTTTGATACCTTAAACGAAGAGCAATATTACAGTTTCTTCCTCAGT
GTGCTCGAAGTGTACGGTTTCGCCGTCGTTGAAATGGATAATGGCGTACTGAAAGTCATCAAATCGAAAGATGCAAAGAC
CTCAGCCATTCCGGTGTTGAGTGGTGAAGAGCGCGCCAATGGCGATGAAGTCATTACCCAAGTGGTCGCGGTGAAAAACG
TTTCCGTACGCGAGTTGTCCCCTTTGCTGCGCCAACTGATTGATAACGCAGGGGCGGGGAACGTAGTGCACTACGATCCG
GCGAACATCATTTTGATCACGGGGCGAGCTGCGGTAGTGAACCGTTTAGCGGAAATTATTCGTCGCGTTGACCAAGCCGG
TGACAAAGAGATTGAAGTGGTTGAGCTTAATAATGCATCGGCAGCTGAAATGGTGCGGATTGTTGAAGCGCTCAACAAAA
CGACAGACGCGCAAAACACCCCTGAATTCTTAAAGCCCAAGTTTGTGGCAGACGAGCGTACCAACTCGATTTTGATTTCT
GGCGATCCTAAAGTGCGCGAGCGCCTCAAGCGTCTGATCAAGCAGTTGGATGTTGAGATGGCGGCCAAAGGCAATAACCG
CGTGGTGTATTTGAAATACGCCAAAGCTGAAGATCTGGTCGAAGTACTGAAAGGGGTGTCTGAGAACCTGCAAGCGGAAA
AAGGCACCGGACAGCCGACCACTTCAAAACGTAATGAAGTGATGATTGCCGCGCACGCTGACACCAACTCGTTAGTGCTT
ACTGCGCCGCAAGACATTATGAATGCGATGCTGGAAGTGATTGGACAGCTAGATATTCGCCGTGCGCAAGTGTTGATTGA
AGCGCTGATTGTCGAAATGGCAGAGGGCGATGGGATCAACCTTGGTGTGCAGTGGGGCTCGCTGGAAAGTGGTTCAGTTA
TCCAATATGGCAACACTGGCGCGTCGATTGGCAATGTGATGATTGGTCTTGAAGAAGCCAAAGACACAACCCAAACCAAA
GCGGTTTATGATACTAATAACAACTTCTTGAGAAATGAAACGACGACCACCAAAGGGGATTACACCAAGTTAGCCTCTGC
ATTGTCGAGCATTCAAGGTGCTGCAGTCAGCATCGCGATGGGCGACTGGACGGCCTTAATCAACGCGGTCTCTAATGATT
CCAGCTCGAATATCCTGTCATCACCCAGCATTACGGTGATGGATAACGGTGAAGCCTCCTTTATCGTGGGTGAAGAAGTG
CCGGTTATCACAGGTTCTACTGCAGGCTCTAATAACGACAACCCATTCCAAACCGTGGATCGTAAAGAAGTCGGTATCAA
GCTTAAAGTGGTGCCGCAGATCAACGAAGGTAACTCAGTCCAGCTCAATATTGAGCAAGAAGTCTCGAACGTGTTGGGCG
CCAATGGAGCGGTAGACGTGCGTTTTGCGAAGCGTCAGCTCAACACTTCTGTGATGGTGCAAGATGGGCAGATGTTGGTG
CTCGGCGGTTTGATTGATGAGCGAGCTCTTGAGAGTGAGTCGAAAGTCCCGCTCTTGGGGGATATTCCTCTGCTGGGTCA
ACTGTTCCGCTCAACCAGCTCGCAAGTGGAAAAGAAAAACCTGATGGTGTTTATCAAGCCGACCATTATTCGTGATGGCG
TGACGGCCGATGGCATCACCCAACGTAAATACAACTACATCCGCGCCGAGCAACTGTTCCGCGCCGAAAAAGGTTTACGT
CTGCTGGATGATGCTAGCGTGCCTGTGTTGCCGAAATTTGGCGATGACCGCCGCCATTCACCTGAAATTCAAGCCTTTAT
TGAGCAGATGGAAGCCAAGCAATGA

Upstream 100 bases:

>100_bases
GAGTCTGACCGTTGAGCGTGATGGTCAACAACATGATGTATATATTCAATTTTAACGCTAAGGCGAGTAACGACGCCTTA
GTGAGGCAAGGGAGTTCCCA

Downstream 100 bases:

>100_bases
CCGAAATGGTGATCTCTCCAGCTGAGCGACAGTCGATTCGTCGTCTGCCCTTTAGCTTCGCCAATCGCTTTAAGTTGGTG
CTGGATTGGAATGAGGATTT

Product: general secretion pathway protein D

Products: NA

Alternate protein names: Cholera toxin secretion protein epsD

Number of amino acids: Translated: 674; Mature: 674

Protein sequence:

>674_residues
MKYWLKKSSWLLAGSLLSTPLAMANEFSASFKGTDIQEFINIVGRNLEKTIIVDPSVRGKVDVRSFDTLNEEQYYSFFLS
VLEVYGFAVVEMDNGVLKVIKSKDAKTSAIPVLSGEERANGDEVITQVVAVKNVSVRELSPLLRQLIDNAGAGNVVHYDP
ANIILITGRAAVVNRLAEIIRRVDQAGDKEIEVVELNNASAAEMVRIVEALNKTTDAQNTPEFLKPKFVADERTNSILIS
GDPKVRERLKRLIKQLDVEMAAKGNNRVVYLKYAKAEDLVEVLKGVSENLQAEKGTGQPTTSKRNEVMIAAHADTNSLVL
TAPQDIMNAMLEVIGQLDIRRAQVLIEALIVEMAEGDGINLGVQWGSLESGSVIQYGNTGASIGNVMIGLEEAKDTTQTK
AVYDTNNNFLRNETTTTKGDYTKLASALSSIQGAAVSIAMGDWTALINAVSNDSSSNILSSPSITVMDNGEASFIVGEEV
PVITGSTAGSNNDNPFQTVDRKEVGIKLKVVPQINEGNSVQLNIEQEVSNVLGANGAVDVRFAKRQLNTSVMVQDGQMLV
LGGLIDERALESESKVPLLGDIPLLGQLFRSTSSQVEKKNLMVFIKPTIIRDGVTADGITQRKYNYIRAEQLFRAEKGLR
LLDDASVPVLPKFGDDRRHSPEIQAFIEQMEAKQ

Sequences:

>Translated_674_residues
MKYWLKKSSWLLAGSLLSTPLAMANEFSASFKGTDIQEFINIVGRNLEKTIIVDPSVRGKVDVRSFDTLNEEQYYSFFLS
VLEVYGFAVVEMDNGVLKVIKSKDAKTSAIPVLSGEERANGDEVITQVVAVKNVSVRELSPLLRQLIDNAGAGNVVHYDP
ANIILITGRAAVVNRLAEIIRRVDQAGDKEIEVVELNNASAAEMVRIVEALNKTTDAQNTPEFLKPKFVADERTNSILIS
GDPKVRERLKRLIKQLDVEMAAKGNNRVVYLKYAKAEDLVEVLKGVSENLQAEKGTGQPTTSKRNEVMIAAHADTNSLVL
TAPQDIMNAMLEVIGQLDIRRAQVLIEALIVEMAEGDGINLGVQWGSLESGSVIQYGNTGASIGNVMIGLEEAKDTTQTK
AVYDTNNNFLRNETTTTKGDYTKLASALSSIQGAAVSIAMGDWTALINAVSNDSSSNILSSPSITVMDNGEASFIVGEEV
PVITGSTAGSNNDNPFQTVDRKEVGIKLKVVPQINEGNSVQLNIEQEVSNVLGANGAVDVRFAKRQLNTSVMVQDGQMLV
LGGLIDERALESESKVPLLGDIPLLGQLFRSTSSQVEKKNLMVFIKPTIIRDGVTADGITQRKYNYIRAEQLFRAEKGLR
LLDDASVPVLPKFGDDRRHSPEIQAFIEQMEAKQ
>Mature_674_residues
MKYWLKKSSWLLAGSLLSTPLAMANEFSASFKGTDIQEFINIVGRNLEKTIIVDPSVRGKVDVRSFDTLNEEQYYSFFLS
VLEVYGFAVVEMDNGVLKVIKSKDAKTSAIPVLSGEERANGDEVITQVVAVKNVSVRELSPLLRQLIDNAGAGNVVHYDP
ANIILITGRAAVVNRLAEIIRRVDQAGDKEIEVVELNNASAAEMVRIVEALNKTTDAQNTPEFLKPKFVADERTNSILIS
GDPKVRERLKRLIKQLDVEMAAKGNNRVVYLKYAKAEDLVEVLKGVSENLQAEKGTGQPTTSKRNEVMIAAHADTNSLVL
TAPQDIMNAMLEVIGQLDIRRAQVLIEALIVEMAEGDGINLGVQWGSLESGSVIQYGNTGASIGNVMIGLEEAKDTTQTK
AVYDTNNNFLRNETTTTKGDYTKLASALSSIQGAAVSIAMGDWTALINAVSNDSSSNILSSPSITVMDNGEASFIVGEEV
PVITGSTAGSNNDNPFQTVDRKEVGIKLKVVPQINEGNSVQLNIEQEVSNVLGANGAVDVRFAKRQLNTSVMVQDGQMLV
LGGLIDERALESESKVPLLGDIPLLGQLFRSTSSQVEKKNLMVFIKPTIIRDGVTADGITQRKYNYIRAEQLFRAEKGLR
LLDDASVPVLPKFGDDRRHSPEIQAFIEQMEAKQ

Specific function: Required for secretion of cholera toxin through the outer membrane

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family

Homologues:

Organism=Escherichia coli, GI87082242, Length=656, Percent_Identity=44.6646341463415, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI1789793, Length=349, Percent_Identity=25.7879656160458, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSPD_VIBCH (P45779)

Other databases:

- EMBL:   L33796
- EMBL:   AE003852
- GenomeReviews:   AE003852_GR
- TIGR:   VC_2733
- HOGENOM:   HBG691281
- OMA:   GYAVVEM
- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR004846
- InterPro:   IPR013356
- InterPro:   IPR004845
- InterPro:   IPR001814
- PRINTS:   PR00811
- PRINTS:   PR01032
- TIGRFAMs:   TIGR02517

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N

EC number: NA

Molecular weight: Translated: 73471; Mature: 73471

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00875 T2SP_D

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYWLKKSSWLLAGSLLSTPLAMANEFSASFKGTDIQEFINIVGRNLEKTIIVDPSVRGK
CCCEECCCCEEEECHHHHCHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCE
VDVRSFDTLNEEQYYSFFLSVLEVYGFAVVEMDNGVLKVIKSKDAKTSAIPVLSGEERAN
EEECCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCCEEEEECCCCCCCEEEEECCCCCCC
GDEVITQVVAVKNVSVRELSPLLRQLIDNAGAGNVVHYDPANIILITGRAAVVNRLAEII
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCHHHHHHHHHHH
RRVDQAGDKEIEVVELNNASAAEMVRIVEALNKTTDAQNTPEFLKPKFVADERTNSILIS
HHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCEECCCCCCEEEEE
GDPKVRERLKRLIKQLDVEMAAKGNNRVVYLKYAKAEDLVEVLKGVSENLQAEKGTGQPT
CCHHHHHHHHHHHHHHCHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCC
TSKRNEVMIAAHADTNSLVLTAPQDIMNAMLEVIGQLDIRRAQVLIEALIVEMAEGDGIN
CCCCCEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LGVQWGSLESGSVIQYGNTGASIGNVMIGLEEAKDTTQTKAVYDTNNNFLRNETTTTKGD
EEEEECCCCCCCEEEECCCCCCCCCEEEEEHHCCCCCCCEEEEECCCCEEECCCCCCCCH
YTKLASALSSIQGAAVSIAMGDWTALINAVSNDSSSNILSSPSITVMDNGEASFIVGEEV
HHHHHHHHHHCCCCEEEEEECHHHHHHHHHCCCCCCCEECCCCEEEEECCCEEEEECCCC
PVITGSTAGSNNDNPFQTVDRKEVGIKLKVVPQINEGNSVQLNIEQEVSNVLGANGAVDV
CEEECCCCCCCCCCCCCCCCHHHCCEEEEEEEECCCCCEEEEEEHHHHHHHHCCCCCEEE
RFAKRQLNTSVMVQDGQMLVLGGLIDERALESESKVPLLGDIPLLGQLFRSTSSQVEKKN
EEHHHHCCCEEEEECCCEEEEECHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHCCCC
LMVFIKPTIIRDGVTADGITQRKYNYIRAEQLFRAEKGLRLLDDASVPVLPKFGDDRRHS
EEEEEECEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCC
PEIQAFIEQMEAKQ
CHHHHHHHHHHCCC
>Mature Secondary Structure
MKYWLKKSSWLLAGSLLSTPLAMANEFSASFKGTDIQEFINIVGRNLEKTIIVDPSVRGK
CCCEECCCCEEEECHHHHCHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCE
VDVRSFDTLNEEQYYSFFLSVLEVYGFAVVEMDNGVLKVIKSKDAKTSAIPVLSGEERAN
EEECCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCCEEEEECCCCCCCEEEEECCCCCCC
GDEVITQVVAVKNVSVRELSPLLRQLIDNAGAGNVVHYDPANIILITGRAAVVNRLAEII
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEECCHHHHHHHHHHH
RRVDQAGDKEIEVVELNNASAAEMVRIVEALNKTTDAQNTPEFLKPKFVADERTNSILIS
HHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHCCCEECCCCCCEEEEE
GDPKVRERLKRLIKQLDVEMAAKGNNRVVYLKYAKAEDLVEVLKGVSENLQAEKGTGQPT
CCHHHHHHHHHHHHHHCHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCC
TSKRNEVMIAAHADTNSLVLTAPQDIMNAMLEVIGQLDIRRAQVLIEALIVEMAEGDGIN
CCCCCEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LGVQWGSLESGSVIQYGNTGASIGNVMIGLEEAKDTTQTKAVYDTNNNFLRNETTTTKGD
EEEEECCCCCCCEEEECCCCCCCCCEEEEEHHCCCCCCCEEEEECCCCEEECCCCCCCCH
YTKLASALSSIQGAAVSIAMGDWTALINAVSNDSSSNILSSPSITVMDNGEASFIVGEEV
HHHHHHHHHHCCCCEEEEEECHHHHHHHHHCCCCCCCEECCCCEEEEECCCEEEEECCCC
PVITGSTAGSNNDNPFQTVDRKEVGIKLKVVPQINEGNSVQLNIEQEVSNVLGANGAVDV
CEEECCCCCCCCCCCCCCCCHHHCCEEEEEEEECCCCCEEEEEEHHHHHHHHCCCCCEEE
RFAKRQLNTSVMVQDGQMLVLGGLIDERALESESKVPLLGDIPLLGQLFRSTSSQVEKKN
EEHHHHCCCEEEEECCCEEEEECHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHCCCC
LMVFIKPTIIRDGVTADGITQRKYNYIRAEQLFRAEKGLRLLDDASVPVLPKFGDDRRHS
EEEEEECEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCC
PEIQAFIEQMEAKQ
CHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301