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The map label for this gene is petB [H]

Identifier: 147674128

GI number: 147674128

Start: 95597

End: 96862

Strand: Direct

Name: petB [H]

Synonym: VC0395_A0106

Alternate gene names: 147674128

Gene position: 95597-96862 (Clockwise)

Preceding gene: 147673531

Following gene: 147673543

Centisome position: 3.16

GC content: 44.23

Gene sequence:

>1266_bases
ATGCAAGCTCTACTTGATTGGGTAGAAAAACGCCTACCTGCCATGAATGCGTACAAGAAACACCTTTCAGAATACCCAAT
GCCAAAGAACTTTAACTTTTGGTATCTGTTTGGTTCGCTTGCGATGTTGGTTTTGGTCAACCAACTCCTGACCGGTATTT
GGTTAACCATGAACTATGTGCCATCGGGAGAGGGCGCTTTTGCTTCGATTGAATACATCATGCGTGATGTGGAGTACGGC
TGGTTATTGCGTTATATGCATTCAACCGGGGCTTCTGCATTTTTCGTGGTAGTTTATCTGCACATGTTCCGTGGCTTGAT
TTATGGATCATACCAAAAGCCTCGTGAGCTATTGTGGATCTTCGGTATGCTGATTTTCCTTGTGTTGATGGCAGAAGCCT
TTATGGGCTACTTGTTACCTTGGGGACAGATGTCTTACTGGGGCGCACAGGTAATCATTTCGCTGTTTGGTGCAATTCCG
GTTATTGGTGATGATCTCACGCTGTGGATCCGTGGTGACTATGTCATTTCTGGTGCCACACTAAACCGTTTCTTTGCTTT
GCATGTGATTGCGTTGCCAATCGTATTACTGCTTTTGATCGTGCTGCATATTCTGGCTCTGCACGAAGTTGGCTCAAATA
ACCCTGATGGTATTGAGACTAAATTGCCAAAAGGTTCAATGGGTGAAGGCTACGAATCACAGTTTAAATTCCATGAGTAC
TACACTAAGAAGTACGACATTATCGATTCGATCCCATTCCATCCTTATGGCACGGTGAAAGATTTAATTGGTGTTGCGGG
ATTCTTATTCTTGTTCTGCTATGTGTTGTTCTTCAATCCAGAGATGGGAGGATACTTCCTTGAGCCGCCCAACTTTGAAG
CAGCTAACCCACTGAAAACACCAGAACATATTGCGCCTGTTTGGTACTTTACGCCGTTTTACGCCATCTTGCGTGCGGTT
CCAGATAAGCTGCTAGGGGTTATCGCGATGGGGGCATCGATTGTTGTATTGTTCCTGTTACCTTGGCTGGATCGTTGTAA
GGTTCGTTCTTACCGTTATCGTAGCAAATTCCATTTATTCAATATTGCCCAGTTCACTGTTAGCTTTATTGCCTTAGGGA
TTTTAGGCGCGCTACCTGCAACTCCAACCTACACACTGTTGGCTCAGATATTTAGCTTGTGTTACTTCATGTTCTTTGTG
TTGCTGTTCTTCTACAGCAAAAACGAAGCCACTAAGCCATTACCAACAAGGGTGACATACAAATGA

Upstream 100 bases:

>100_bases
TCCAAGCTGTTCCGGCACCACTAAATCTTGTTATCCCGCCGCACATGTATCTCAGTGATACGCGGATCGTGATTGGTCTT
GATGAGACAGGGGAGGCATA

Downstream 100 bases:

>100_bases
AAAAATGGATTGTAGTTTTGTTTGCTATGCTGCCTTCACTGGCGATGGCGGCTGGAGCAAATGTGCATTTAGATAAAGCG
AATAACGATTTGACGGATCA

Product: ubiquinol--cytochrome c reductase, cytochrome B

Products: Q; ferrocytochrome c

Alternate protein names: NA

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MQALLDWVEKRLPAMNAYKKHLSEYPMPKNFNFWYLFGSLAMLVLVNQLLTGIWLTMNYVPSGEGAFASIEYIMRDVEYG
WLLRYMHSTGASAFFVVVYLHMFRGLIYGSYQKPRELLWIFGMLIFLVLMAEAFMGYLLPWGQMSYWGAQVIISLFGAIP
VIGDDLTLWIRGDYVISGATLNRFFALHVIALPIVLLLLIVLHILALHEVGSNNPDGIETKLPKGSMGEGYESQFKFHEY
YTKKYDIIDSIPFHPYGTVKDLIGVAGFLFLFCYVLFFNPEMGGYFLEPPNFEAANPLKTPEHIAPVWYFTPFYAILRAV
PDKLLGVIAMGASIVVLFLLPWLDRCKVRSYRYRSKFHLFNIAQFTVSFIALGILGALPATPTYTLLAQIFSLCYFMFFV
LLFFYSKNEATKPLPTRVTYK

Sequences:

>Translated_421_residues
MQALLDWVEKRLPAMNAYKKHLSEYPMPKNFNFWYLFGSLAMLVLVNQLLTGIWLTMNYVPSGEGAFASIEYIMRDVEYG
WLLRYMHSTGASAFFVVVYLHMFRGLIYGSYQKPRELLWIFGMLIFLVLMAEAFMGYLLPWGQMSYWGAQVIISLFGAIP
VIGDDLTLWIRGDYVISGATLNRFFALHVIALPIVLLLLIVLHILALHEVGSNNPDGIETKLPKGSMGEGYESQFKFHEY
YTKKYDIIDSIPFHPYGTVKDLIGVAGFLFLFCYVLFFNPEMGGYFLEPPNFEAANPLKTPEHIAPVWYFTPFYAILRAV
PDKLLGVIAMGASIVVLFLLPWLDRCKVRSYRYRSKFHLFNIAQFTVSFIALGILGALPATPTYTLLAQIFSLCYFMFFV
LLFFYSKNEATKPLPTRVTYK
>Mature_421_residues
MQALLDWVEKRLPAMNAYKKHLSEYPMPKNFNFWYLFGSLAMLVLVNQLLTGIWLTMNYVPSGEGAFASIEYIMRDVEYG
WLLRYMHSTGASAFFVVVYLHMFRGLIYGSYQKPRELLWIFGMLIFLVLMAEAFMGYLLPWGQMSYWGAQVIISLFGAIP
VIGDDLTLWIRGDYVISGATLNRFFALHVIALPIVLLLLIVLHILALHEVGSNNPDGIETKLPKGSMGEGYESQFKFHEY
YTKKYDIIDSIPFHPYGTVKDLIGVAGFLFLFCYVLFFNPEMGGYFLEPPNFEAANPLKTPEHIAPVWYFTPFYAILRAV
PDKLLGVIAMGASIVVLFLLPWLDRCKVRSYRYRSKFHLFNIAQFTVSFIALGILGALPATPTYTLLAQIFSLCYFMFFV
LLFFYSKNEATKPLPTRVTYK

Specific function: Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis [H]

COG id: COG1290

COG function: function code C; Cytochrome b subunit of the bc complex

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome b family [H]

Homologues:

Organism=Homo sapiens, GI251831119, Length=400, Percent_Identity=40.5, Blast_Score=264, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6226529, Length=386, Percent_Identity=41.7098445595855, Blast_Score=277, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6226532, Length=273, Percent_Identity=40.6593406593407, Blast_Score=200, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6226531, Length=161, Percent_Identity=48.4472049689441, Blast_Score=150, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6226530, Length=152, Percent_Identity=46.0526315789474, Blast_Score=126, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016175
- InterPro:   IPR005798
- InterPro:   IPR005797
- InterPro:   IPR016174 [H]

Pfam domain/function: PF00032 Cytochrom_B_C; PF00033 Cytochrom_B_N [H]

EC number: 1.10.2.2

Molecular weight: Translated: 48301; Mature: 48301

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS51002 CYTB_NTER ; PS51003 CYTB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQALLDWVEKRLPAMNAYKKHLSEYPMPKNFNFWYLFGSLAMLVLVNQLLTGIWLTMNYV
CCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEC
PSGEGAFASIEYIMRDVEYGWLLRYMHSTGASAFFVVVYLHMFRGLIYGSYQKPRELLWI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FGMLIFLVLMAEAFMGYLLPWGQMSYWGAQVIISLFGAIPVIGDDLTLWIRGDYVISGAT
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEECCCEEEEEECCEEEECHH
LNRFFALHVIALPIVLLLLIVLHILALHEVGSNNPDGIETKLPKGSMGEGYESQFKFHEY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
YTKKYDIIDSIPFHPYGTVKDLIGVAGFLFLFCYVLFFNPEMGGYFLEPPNFEAANPLKT
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCC
PEHIAPVWYFTPFYAILRAVPDKLLGVIAMGASIVVLFLLPWLDRCKVRSYRYRSKFHLF
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIAQFTVSFIALGILGALPATPTYTLLAQIFSLCYFMFFVLLFFYSKNEATKPLPTRVTY
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
K
C
>Mature Secondary Structure
MQALLDWVEKRLPAMNAYKKHLSEYPMPKNFNFWYLFGSLAMLVLVNQLLTGIWLTMNYV
CCHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEC
PSGEGAFASIEYIMRDVEYGWLLRYMHSTGASAFFVVVYLHMFRGLIYGSYQKPRELLWI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
FGMLIFLVLMAEAFMGYLLPWGQMSYWGAQVIISLFGAIPVIGDDLTLWIRGDYVISGAT
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEECCCEEEEEECCEEEECHH
LNRFFALHVIALPIVLLLLIVLHILALHEVGSNNPDGIETKLPKGSMGEGYESQFKFHEY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
YTKKYDIIDSIPFHPYGTVKDLIGVAGFLFLFCYVLFFNPEMGGYFLEPPNFEAANPLKT
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCCC
PEHIAPVWYFTPFYAILRAVPDKLLGVIAMGASIVVLFLLPWLDRCKVRSYRYRSKFHLF
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NIAQFTVSFIALGILGALPATPTYTLLAQIFSLCYFMFFVLLFFYSKNEATKPLPTRVTY
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: QH2; ferricytochrome c

Specific reaction: QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA