Click here to switch to the map view.

The map label for this gene is vacJ [H]

Identifier: 147674073

GI number: 147674073

Start: 1758546

End: 1759310

Strand: Reverse

Name: vacJ [H]

Synonym: VC0395_A1636

Alternate gene names: 147674073

Gene position: 1759310-1758546 (Counterclockwise)

Preceding gene: 147675723

Following gene: 147675696

Centisome position: 58.18

GC content: 47.32

Gene sequence:

>765_bases
TTGCACATGGAATTACGTATGGTTGGCCGCTTATCTTGGATTACGCTATTCACACTTTTTTTAGTTGGGTGCAGCAGTGC
ACCTGATGACTCCTCCCCTCATTCGCAGGTGAACGATCCTCTGGAAAGTTTCAACCGGCAAATGTGGACAATTAACTATG
ACTACCTAGACCCTTATGTGGTGCGTCCGGTCTCTCTATTTTATGTCGGTTATGTACCTAAGCCTGTACGCAGTGGCATT
GCCAACTTCCTCTCTAACTTAGACGAGCCTGCCAGCATGGTGAATAACCTGCTGATGGGCAATGGGACAAAAGCGGTCGA
TCACTTTAATCGTTTTTGGATTAATACCAGCTTTGGTTTACTCGGTTTGATTGATATCGCTTCTGAAGCAGGCATCAAAA
AATACGATGATAAGGCGTTCAGTGATGCGGTAGGCCATTACGGTGTGGGCAATGGCCCCTATTTAATGGTCCCAGGTTAT
GGTCCCTATACGGTACGCGAAGTGACCGATGTGGTGGATGGCATGTATTTCCCGCTTGCCTATCTCAATATCTGGGCTGG
GGTCGGCAAATGGGCACTTGAAGGCATGGAAACGCGCGCTGCGTTAGTTTCGCAAGAGGCCTTATTACAAGACTCACCTG
ATCCTTACAGTTTGGCTCGCGATGCTTATCTCCAACGGCAAGCTTTCAAAGCGGAGATCCAAGTGGATGACTATGACCCT
GAGGAAGAAGAGTATCTCGATGAGTATTTAAATGAAGGGTTATGA

Upstream 100 bases:

>100_bases
AAAACAGGTGAGCCTGTTGACGTTGTGCTTGATAAGCAGTATGAATAAAATCACAAGCTGAAAAATAATATAACAATAAG
GCCGATGGGATCATCGGCCT

Downstream 100 bases:

>100_bases
TGGTCTCTGAAATCAGGCTGACATAACCCTGCCGCTAAAGTATTCATTGGAAACAATATACTCTGTATTACGGATCAGCT
CTTCCCGCATTTCTGACCAG

Product: vacJ lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MHMELRMVGRLSWITLFTLFLVGCSSAPDDSSPHSQVNDPLESFNRQMWTINYDYLDPYVVRPVSLFYVGYVPKPVRSGI
ANFLSNLDEPASMVNNLLMGNGTKAVDHFNRFWINTSFGLLGLIDIASEAGIKKYDDKAFSDAVGHYGVGNGPYLMVPGY
GPYTVREVTDVVDGMYFPLAYLNIWAGVGKWALEGMETRAALVSQEALLQDSPDPYSLARDAYLQRQAFKAEIQVDDYDP
EEEEYLDEYLNEGL

Sequences:

>Translated_254_residues
MHMELRMVGRLSWITLFTLFLVGCSSAPDDSSPHSQVNDPLESFNRQMWTINYDYLDPYVVRPVSLFYVGYVPKPVRSGI
ANFLSNLDEPASMVNNLLMGNGTKAVDHFNRFWINTSFGLLGLIDIASEAGIKKYDDKAFSDAVGHYGVGNGPYLMVPGY
GPYTVREVTDVVDGMYFPLAYLNIWAGVGKWALEGMETRAALVSQEALLQDSPDPYSLARDAYLQRQAFKAEIQVDDYDP
EEEEYLDEYLNEGL
>Mature_254_residues
MHMELRMVGRLSWITLFTLFLVGCSSAPDDSSPHSQVNDPLESFNRQMWTINYDYLDPYVVRPVSLFYVGYVPKPVRSGI
ANFLSNLDEPASMVNNLLMGNGTKAVDHFNRFWINTSFGLLGLIDIASEAGIKKYDDKAFSDAVGHYGVGNGPYLMVPGY
GPYTVREVTDVVDGMYFPLAYLNIWAGVGKWALEGMETRAALVSQEALLQDSPDPYSLARDAYLQRQAFKAEIQVDDYDP
EEEEYLDEYLNEGL

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=225, Percent_Identity=46.6666666666667, Blast_Score=205, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 28574; Mature: 28574

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHMELRMVGRLSWITLFTLFLVGCSSAPDDSSPHSQVNDPLESFNRQMWTINYDYLDPYV
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCHH
VRPVSLFYVGYVPKPVRSGIANFLSNLDEPASMVNNLLMGNGTKAVDHFNRFWINTSFGL
HHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCH
LGLIDIASEAGIKKYDDKAFSDAVGHYGVGNGPYLMVPGYGPYTVREVTDVVDGMYFPLA
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
YLNIWAGVGKWALEGMETRAALVSQEALLQDSPDPYSLARDAYLQRQAFKAEIQVDDYDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECCCCC
EEEEYLDEYLNEGL
CHHHHHHHHHHCCC
>Mature Secondary Structure
MHMELRMVGRLSWITLFTLFLVGCSSAPDDSSPHSQVNDPLESFNRQMWTINYDYLDPYV
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCHH
VRPVSLFYVGYVPKPVRSGIANFLSNLDEPASMVNNLLMGNGTKAVDHFNRFWINTSFGL
HHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHEEECCCCH
LGLIDIASEAGIKKYDDKAFSDAVGHYGVGNGPYLMVPGYGPYTVREVTDVVDGMYFPLA
HHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
YLNIWAGVGKWALEGMETRAALVSQEALLQDSPDPYSLARDAYLQRQAFKAEIQVDDYDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEECCCCC
EEEEYLDEYLNEGL
CHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]