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The map label for this gene is hisI

Identifier: 147673838

GI number: 147673838

Start: 732389

End: 733021

Strand: Direct

Name: hisI

Synonym: VC0395_A0709

Alternate gene names: 147673838

Gene position: 732389-733021 (Clockwise)

Preceding gene: 147674704

Following gene: 147673535

Centisome position: 24.22

GC content: 49.76

Gene sequence:

>633_bases
ATGAATCCAGCAAGCCCATTTGCCACGCTAACCGACCGTATTGATTGGCAGAAAGTCGATGGACTTGTGCCCGCCATTGT
GCAAGATTTTCAGTCCAGCCAAGTCTTAATGATGGGGTATATGAACCCTGAAGCGTTGCAAAAAACCCTCGATACTCAGC
AAGTGACCTTTTTCTCGCGCAGCAAACAGCGTTTGTGGACTAAGGGAGAAACCTCAGGTCATGTGTTGCAACTGAAAAAT
ATCGCGCTGGATTGCGACCAAGATACGCTACTGGTGAAAGTGAACCCGATTGGCCCAACCTGTCACACAGGCACCGTGAC
CTGTTGGGATGGTGATGCACAAGAGGAGTCACAAATGGTTTGGCTTCATCAGTTGGAACAACTGTTGGCAGAGCGCAAAA
ATGCCGATCCCAGTTCTTCTTACACGGCCTCTTTGTATGCGCGTGGCACCAAACGCATTGCGCAGAAAGTGGGTGAAGAA
GGGGTTGAAGTCGCGTTGGCGGCGACGGCAGGGGATAAGGAGGAGCTGATTTGCGAATCCGCCGACTTGATGTATCACTT
GTTGGTGTTGCTGCAAGAGCAAGGCTTAGCCATGAATGATGTGATCAATAAGCTGAAAGAACGCCATAAGTAA

Upstream 100 bases:

>100_bases
ACGTCGATGGTGCTTTAGCCGCTTCGGTGTTTCACAAGCAGATCATCAATATCGGTGAACTCAAGCAGTATCTAAAACAA
CAAGGAATTGAGGTGAGACG

Downstream 100 bases:

>100_bases
TTGGTAGACGAGTTTCTCCAACCAAACAATAACGCCCCATTATTGGGGCGTTATTGCCTTCCTACTTGAAGCTGCAGCGG
TGTTGGCTGCATTCGTTCAC

Product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein

Products: NA

Alternate protein names: Phosphoribosyl-AMP cyclohydrolase; PRA-CH; Phosphoribosyl-ATP pyrophosphatase; PRA-PH

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MNPASPFATLTDRIDWQKVDGLVPAIVQDFQSSQVLMMGYMNPEALQKTLDTQQVTFFSRSKQRLWTKGETSGHVLQLKN
IALDCDQDTLLVKVNPIGPTCHTGTVTCWDGDAQEESQMVWLHQLEQLLAERKNADPSSSYTASLYARGTKRIAQKVGEE
GVEVALAATAGDKEELICESADLMYHLLVLLQEQGLAMNDVINKLKERHK

Sequences:

>Translated_210_residues
MNPASPFATLTDRIDWQKVDGLVPAIVQDFQSSQVLMMGYMNPEALQKTLDTQQVTFFSRSKQRLWTKGETSGHVLQLKN
IALDCDQDTLLVKVNPIGPTCHTGTVTCWDGDAQEESQMVWLHQLEQLLAERKNADPSSSYTASLYARGTKRIAQKVGEE
GVEVALAATAGDKEELICESADLMYHLLVLLQEQGLAMNDVINKLKERHK
>Mature_210_residues
MNPASPFATLTDRIDWQKVDGLVPAIVQDFQSSQVLMMGYMNPEALQKTLDTQQVTFFSRSKQRLWTKGETSGHVLQLKN
IALDCDQDTLLVKVNPIGPTCHTGTVTCWDGDAQEESQMVWLHQLEQLLAERKNADPSSSYTASLYARGTKRIAQKVGEE
GVEVALAATAGDKEELICESADLMYHLLVLLQEQGLAMNDVINKLKERHK

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PRA-PH family

Homologues:

Organism=Escherichia coli, GI2367128, Length=197, Percent_Identity=66.497461928934, Blast_Score=269, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6319819, Length=192, Percent_Identity=33.8541666666667, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS2_VIBCH (Q9KSW7)

Other databases:

- EMBL:   AE003852
- PIR:   F82238
- RefSeq:   NP_230784.1
- ProteinModelPortal:   Q9KSW7
- GeneID:   2614409
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC1139
- TIGR:   VC_1139
- HOGENOM:   HBG294308
- OMA:   VMACKDD
- ProtClustDB:   PRK02759
- BRENDA:   3.5.4.19
- BRENDA:   3.6.1.31
- GO:   GO:0005737
- HAMAP:   MF_01019
- InterPro:   IPR023019
- InterPro:   IPR008179
- InterPro:   IPR021130
- InterPro:   IPR002496
- ProDom:   PD002610
- TIGRFAMs:   TIGR03188

Pfam domain/function: PF01502 PRA-CH; PF01503 PRA-PH

EC number: =3.5.4.19; =3.6.1.31

Molecular weight: Translated: 23419; Mature: 23419

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPASPFATLTDRIDWQKVDGLVPAIVQDFQSSQVLMMGYMNPEALQKTLDTQQVTFFSR
CCCCCCHHHHHHCCCHHHHHCHHHHHHHHHCCCCEEEEECCCHHHHHHHHCHHHHHHHHH
SKQRLWTKGETSGHVLQLKNIALDCDQDTLLVKVNPIGPTCHTGTVTCWDGDAQEESQMV
HHHHHCCCCCCCCCEEEEECEEEECCCCEEEEEEECCCCCCCCCEEEEECCCCCHHHHHH
WLHQLEQLLAERKNADPSSSYTASLYARGTKRIAQKVGEEGVEVALAATAGDKEELICES
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
ADLMYHLLVLLQEQGLAMNDVINKLKERHK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MNPASPFATLTDRIDWQKVDGLVPAIVQDFQSSQVLMMGYMNPEALQKTLDTQQVTFFSR
CCCCCCHHHHHHCCCHHHHHCHHHHHHHHHCCCCEEEEECCCHHHHHHHHCHHHHHHHHH
SKQRLWTKGETSGHVLQLKNIALDCDQDTLLVKVNPIGPTCHTGTVTCWDGDAQEESQMV
HHHHHCCCCCCCCCEEEEECEEEECCCCEEEEEEECCCCCCCCCEEEEECCCCCHHHHHH
WLHQLEQLLAERKNADPSSSYTASLYARGTKRIAQKVGEEGVEVALAATAGDKEELICES
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHH
ADLMYHLLVLLQEQGLAMNDVINKLKERHK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301