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The map label for this gene is pheT [H]

Identifier: 147673806

GI number: 147673806

Start: 855920

End: 858307

Strand: Direct

Name: pheT [H]

Synonym: VC0395_A0844

Alternate gene names: 147673806

Gene position: 855920-858307 (Clockwise)

Preceding gene: 147673076

Following gene: 147674752

Centisome position: 28.3

GC content: 52.01

Gene sequence:

>2388_bases
ATGAAATTCAGCGAATCATGGCTTCGTGAGTGGGTTAACCCGGCAATTACCACTGACGAATTAACTCACCAAATTACAAT
GGCGGGTCTGGAAGTGGATGACGTACTGGCCGTAGCTGGTGTGTTCGACGGCGTAAAAGTGGGTCACGTGGTTGAGTGTG
CTCAACATCCAGATGCTGACAAACTGCGCGTCACCAAAGTCGATGTAGGCGAAGAAGAGCTTCTGGACATCGTGTGTGGT
GCAGCCAACTGTCGTCAAGGTCTGAAAGTTGCGGTTGCAACCGTAGGCGCGACGCTGCCAGGCGATTTCAAGATTAAAAA
AGCTAAACTGCGTGGCCAACCGTCACACGGCATGCTGTGTTCTTTCTCTGAGCTGGGTATCGATGTTGAATCGAACGGCA
TCATGGAACTGGCTGAAAACGCACCGATTGGTATGGATTTCCGCGATTTTCTGTCACTGAACGATGTGACTATCGATGTG
GATTTGACGTCTAACCGCGCGGACTGTTTCAGCATCCGTGGTCTGGCTCGTGAAGTGGGCGTATTGAACCGTGCGGACGT
GACTGCTCCTGCGGTGAATGCGATTGTAGCAACCATCAATGATACGATTTCAATTGACGTGAAAGCGCCAGCGGCGTGTC
CGCGTTACCTTGGTCGTATCGTGAAGAACGTGAATGTTCAAGCGCAAACGCCACTCTGGATGCAAGAAAAACTGCGTCGT
TGTGGTATCCGCTCTATCGATCCGGTGGTTGATATCACTAACTTCGTGATGCTGGAGCAAGGCCAACCAATGCACGCGTT
TGATCTGGCGAAGATTGAAGGCGGAATCGTGGTTCGTCTGGCTGAGCAAGATGAAAAACTGACGCTGCTTGATGGCTCAG
AAGCCAAATTAAACGCAGATACGCTAGTGATTGCTGACCAACAAAAAGCGCTGGCGATTGCTGGTGTATTCGGTGGCGAG
CATTCTGGCGTCAGCACTGACACCAAAGATGTATTACTTGAGTGTGCGTTCTTCGCACCAGACCACATTCGTGGTCGTGC
ACGTAGCTACGGCCTACACACCGATTCTTCAATGCGTTTTGAACGTGGTGTGGATTATGCTTTGCAACACGCCGCGATGG
AGCGTGCAACGCAACTGCTGGTTGAGATCTGTGGTGGTGACGTTGCGCCAGTGGTAGCTGCAGAATCTGCGGCCGATTTA
CCAAAACCAAACCAAGTTGCGCTGCGTCGTAGCAAGCTCGACAAACTGCTGGGTCATGCGATTCCGGATGCGGACGTCGT
GGAGATTCTAGAGCGTCTAGGCATGCAGGTCGAGACCACCGCAGAAGGCTGGCAAGCGACCGCGCCAACATGGCGTTTTG
ATATTGCTATCGAGCAAGATCTGGTGGAAGAAGTTGGCCGTATCTATGGTTACAACAACATTCCTAATCAAGCGTCAGTG
GCCGCGTTGAACATGAACCTGCACAACGAAGCGAAATTACCGCTGAAGCGTGTACGCGATCTGCTGGTTGACCGTGGTTA
CCAAGAAGCGATCACCTATAGCTTCGTTGAGCCTGAGCAACAGAAACTGGTTGTACCGGGTGTTGATGCGCTGATCCTGC
CAAACCCAATTTCTGCTGAAATGTCAGCGATGCGTTTGAGCTTGATCCAAGGTCTGCTGAACACTGTGGTTCACAACCAG
AAACGTCAGCAACCTCGTGTTCGTTTGTTTGAATACGGTTTGCGCTTCATTCCTGATGCGGCGGCAGAAAATGGCATGCG
CCAAGAGCCTATGCTGGCGGGTGTGATCTCTGGCGCTCGTGGTGAAGAGCACTGGAATATGGAAACGGCAACCGTTGATT
TCTTCGATATGAAGGGCGATCTGGAAGCGGTTCTTGAACTGACAGCGAAAGGCAAAGCCTATAGCTTCGCAGCAACTAAG
CATCCAGCGCTGCATCCAGGTCAAGCTGCAGCGATTATGGTGGATGGCAAAGCGATCGGTGTTATTGGTACGGTTCACCC
AGAACTTGAGCGTAAGTTTGGCCTCAATGGTCGCACTATTGTGTTCGAGATCGAGTGGAATGCGATCAACACACGCGTGA
TCCCAGAAGCCGCAGCGATTTCTAAGTTCCCAGCCAACCGTCGTGATATCGCGTTGGTGGTCGATGGCAACATCGCTTCT
GGCGACATCGTTGAAGCTTGCCGTGTGGCGGGTGGTGAGCTACTGAAAGACGCAAAACTGTTTGACGTTTATGTCGGTAA
AGGCGTGGAAGAAGGCAAGAAGAGCTTGGCGATTGCGCTGACGTTGCAATCGGTTGAACGCACACTTGAAGAAGCGGATA
TCGCAGCAGCGGTAGAGGCGATTGTGCAAGCCGTATCTGCCCAATTCGGCGCGGCTCTGCGCGACTAA

Upstream 100 bases:

>100_bases
TGCTCCGCTATGGCGTGAATGACCTGCGTGCATTCTTCGAAAACGATCTGCGTTTCCTAAAACAGTTCAAATAATTCAGG
GTTCCCAAGAGGTATATACA

Downstream 100 bases:

>100_bases
GCTTAGGTCTATCTTCGCTTTAAATCAGATGAAAACAGGCTCTTCGGAGCCTGTTTTTTTATCGTGATAATCTGGAACTG
AGCCAACAACTCTCTGATTT

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 795; Mature: 795

Protein sequence:

>795_residues
MKFSESWLREWVNPAITTDELTHQITMAGLEVDDVLAVAGVFDGVKVGHVVECAQHPDADKLRVTKVDVGEEELLDIVCG
AANCRQGLKVAVATVGATLPGDFKIKKAKLRGQPSHGMLCSFSELGIDVESNGIMELAENAPIGMDFRDFLSLNDVTIDV
DLTSNRADCFSIRGLAREVGVLNRADVTAPAVNAIVATINDTISIDVKAPAACPRYLGRIVKNVNVQAQTPLWMQEKLRR
CGIRSIDPVVDITNFVMLEQGQPMHAFDLAKIEGGIVVRLAEQDEKLTLLDGSEAKLNADTLVIADQQKALAIAGVFGGE
HSGVSTDTKDVLLECAFFAPDHIRGRARSYGLHTDSSMRFERGVDYALQHAAMERATQLLVEICGGDVAPVVAAESAADL
PKPNQVALRRSKLDKLLGHAIPDADVVEILERLGMQVETTAEGWQATAPTWRFDIAIEQDLVEEVGRIYGYNNIPNQASV
AALNMNLHNEAKLPLKRVRDLLVDRGYQEAITYSFVEPEQQKLVVPGVDALILPNPISAEMSAMRLSLIQGLLNTVVHNQ
KRQQPRVRLFEYGLRFIPDAAAENGMRQEPMLAGVISGARGEEHWNMETATVDFFDMKGDLEAVLELTAKGKAYSFAATK
HPALHPGQAAAIMVDGKAIGVIGTVHPELERKFGLNGRTIVFEIEWNAINTRVIPEAAAISKFPANRRDIALVVDGNIAS
GDIVEACRVAGGELLKDAKLFDVYVGKGVEEGKKSLAIALTLQSVERTLEEADIAAAVEAIVQAVSAQFGAALRD

Sequences:

>Translated_795_residues
MKFSESWLREWVNPAITTDELTHQITMAGLEVDDVLAVAGVFDGVKVGHVVECAQHPDADKLRVTKVDVGEEELLDIVCG
AANCRQGLKVAVATVGATLPGDFKIKKAKLRGQPSHGMLCSFSELGIDVESNGIMELAENAPIGMDFRDFLSLNDVTIDV
DLTSNRADCFSIRGLAREVGVLNRADVTAPAVNAIVATINDTISIDVKAPAACPRYLGRIVKNVNVQAQTPLWMQEKLRR
CGIRSIDPVVDITNFVMLEQGQPMHAFDLAKIEGGIVVRLAEQDEKLTLLDGSEAKLNADTLVIADQQKALAIAGVFGGE
HSGVSTDTKDVLLECAFFAPDHIRGRARSYGLHTDSSMRFERGVDYALQHAAMERATQLLVEICGGDVAPVVAAESAADL
PKPNQVALRRSKLDKLLGHAIPDADVVEILERLGMQVETTAEGWQATAPTWRFDIAIEQDLVEEVGRIYGYNNIPNQASV
AALNMNLHNEAKLPLKRVRDLLVDRGYQEAITYSFVEPEQQKLVVPGVDALILPNPISAEMSAMRLSLIQGLLNTVVHNQ
KRQQPRVRLFEYGLRFIPDAAAENGMRQEPMLAGVISGARGEEHWNMETATVDFFDMKGDLEAVLELTAKGKAYSFAATK
HPALHPGQAAAIMVDGKAIGVIGTVHPELERKFGLNGRTIVFEIEWNAINTRVIPEAAAISKFPANRRDIALVVDGNIAS
GDIVEACRVAGGELLKDAKLFDVYVGKGVEEGKKSLAIALTLQSVERTLEEADIAAAVEAIVQAVSAQFGAALRD
>Mature_795_residues
MKFSESWLREWVNPAITTDELTHQITMAGLEVDDVLAVAGVFDGVKVGHVVECAQHPDADKLRVTKVDVGEEELLDIVCG
AANCRQGLKVAVATVGATLPGDFKIKKAKLRGQPSHGMLCSFSELGIDVESNGIMELAENAPIGMDFRDFLSLNDVTIDV
DLTSNRADCFSIRGLAREVGVLNRADVTAPAVNAIVATINDTISIDVKAPAACPRYLGRIVKNVNVQAQTPLWMQEKLRR
CGIRSIDPVVDITNFVMLEQGQPMHAFDLAKIEGGIVVRLAEQDEKLTLLDGSEAKLNADTLVIADQQKALAIAGVFGGE
HSGVSTDTKDVLLECAFFAPDHIRGRARSYGLHTDSSMRFERGVDYALQHAAMERATQLLVEICGGDVAPVVAAESAADL
PKPNQVALRRSKLDKLLGHAIPDADVVEILERLGMQVETTAEGWQATAPTWRFDIAIEQDLVEEVGRIYGYNNIPNQASV
AALNMNLHNEAKLPLKRVRDLLVDRGYQEAITYSFVEPEQQKLVVPGVDALILPNPISAEMSAMRLSLIQGLLNTVVHNQ
KRQQPRVRLFEYGLRFIPDAAAENGMRQEPMLAGVISGARGEEHWNMETATVDFFDMKGDLEAVLELTAKGKAYSFAATK
HPALHPGQAAAIMVDGKAIGVIGTVHPELERKFGLNGRTIVFEIEWNAINTRVIPEAAAISKFPANRRDIALVVDGNIAS
GDIVEACRVAGGELLKDAKLFDVYVGKGVEEGKKSLAIALTLQSVERTLEEADIAAAVEAIVQAVSAQFGAALRD

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI124028525, Length=409, Percent_Identity=24.4498777506112, Blast_Score=96, Evalue=2e-19,
Organism=Escherichia coli, GI1788006, Length=795, Percent_Identity=64.4025157232704, Blast_Score=1039, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17533403, Length=560, Percent_Identity=22.6785714285714, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21355211, Length=391, Percent_Identity=26.3427109974425, Blast_Score=104, Evalue=3e-22,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 86125; Mature: 86125

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSESWLREWVNPAITTDELTHQITMAGLEVDDVLAVAGVFDGVKVGHVVECAQHPDAD
CCCCHHHHHHHCCCCCCHHHHHHHHEEECCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
KLRVTKVDVGEEELLDIVCGAANCRQGLKVAVATVGATLPGDFKIKKAKLRGQPSHGMLC
CEEEEEECCCHHHHHHHHHCCHHHHCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCEE
SFSELGIDVESNGIMELAENAPIGMDFRDFLSLNDVTIDVDLTSNRADCFSIRGLAREVG
EHHHHCCEECCCCHHHHHCCCCCCCCHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHC
VLNRADVTAPAVNAIVATINDTISIDVKAPAACPRYLGRIVKNVNVQAQTPLWMQEKLRR
CCCCCCCCHHHHHHEEEEECCEEEEEEECCCCCHHHHHHHHHCCCCEECCCHHHHHHHHH
CGIRSIDPVVDITNFVMLEQGQPMHAFDLAKIEGGIVVRLAEQDEKLTLLDGSEAKLNAD
CCCCCCCHHHHHHHEEEECCCCCCCCEEHEEECCCEEEEEECCCCEEEEEECCCCEECCC
TLVIADQQKALAIAGVFGGEHSGVSTDTKDVLLECAFFAPDHIRGRARSYGLHTDSSMRF
EEEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHCCCHHHCCCCCCCCCHH
ERGVDYALQHAAMERATQLLVEICGGDVAPVVAAESAADLPKPNQVALRRSKLDKLLGHA
HHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHEECCCCCCCCCCHHHHHHHHHHHHHHHHC
IPDADVVEILERLGMQVETTAEGWQATAPTWRFDIAIEQDLVEEVGRIYGYNNIPNQASV
CCCHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCEE
AALNMNLHNEAKLPLKRVRDLLVDRGYQEAITYSFVEPEQQKLVVPGVDALILPNPISAE
EEEEECCCCCCCCCHHHHHHHHHCCCHHHHHEEEECCCCCCEEEECCCCEEEECCCCCHH
MSAMRLSLIQGLLNTVVHNQKRQQPRVRLFEYGLRFIPDAAAENGMRQEPMLAGVISGAR
HHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHCCCC
GEEHWNMETATVDFFDMKGDLEAVLELTAKGKAYSFAATKHPALHPGQAAAIMVDGKAIG
CCCCCCCEEEEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCEEE
VIGTVHPELERKFGLNGRTIVFEIEWNAINTRVIPEAAAISKFPANRRDIALVVDGNIAS
EEEECCHHHHHHHCCCCCEEEEEEEECEECCEECCCHHHHHCCCCCCCCEEEEEECCCCC
GDIVEACRVAGGELLKDAKLFDVYVGKGVEEGKKSLAIALTLQSVERTLEEADIAAAVEA
CHHHHHHHHCCHHHHHCCCEEEEEECCCHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHH
IVQAVSAQFGAALRD
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFSESWLREWVNPAITTDELTHQITMAGLEVDDVLAVAGVFDGVKVGHVVECAQHPDAD
CCCCHHHHHHHCCCCCCHHHHHHHHEEECCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
KLRVTKVDVGEEELLDIVCGAANCRQGLKVAVATVGATLPGDFKIKKAKLRGQPSHGMLC
CEEEEEECCCHHHHHHHHHCCHHHHCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCEE
SFSELGIDVESNGIMELAENAPIGMDFRDFLSLNDVTIDVDLTSNRADCFSIRGLAREVG
EHHHHCCEECCCCHHHHHCCCCCCCCHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHC
VLNRADVTAPAVNAIVATINDTISIDVKAPAACPRYLGRIVKNVNVQAQTPLWMQEKLRR
CCCCCCCCHHHHHHEEEEECCEEEEEEECCCCCHHHHHHHHHCCCCEECCCHHHHHHHHH
CGIRSIDPVVDITNFVMLEQGQPMHAFDLAKIEGGIVVRLAEQDEKLTLLDGSEAKLNAD
CCCCCCCHHHHHHHEEEECCCCCCCCEEHEEECCCEEEEEECCCCEEEEEECCCCEECCC
TLVIADQQKALAIAGVFGGEHSGVSTDTKDVLLECAFFAPDHIRGRARSYGLHTDSSMRF
EEEEECCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHCCCHHHCCCCCCCCCHH
ERGVDYALQHAAMERATQLLVEICGGDVAPVVAAESAADLPKPNQVALRRSKLDKLLGHA
HHCHHHHHHHHHHHHHHHHHHHHHCCCCCHHEECCCCCCCCCCHHHHHHHHHHHHHHHHC
IPDADVVEILERLGMQVETTAEGWQATAPTWRFDIAIEQDLVEEVGRIYGYNNIPNQASV
CCCHHHHHHHHHHCCEEEECCCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCCEE
AALNMNLHNEAKLPLKRVRDLLVDRGYQEAITYSFVEPEQQKLVVPGVDALILPNPISAE
EEEEECCCCCCCCCHHHHHHHHHCCCHHHHHEEEECCCCCCEEEECCCCEEEECCCCCHH
MSAMRLSLIQGLLNTVVHNQKRQQPRVRLFEYGLRFIPDAAAENGMRQEPMLAGVISGAR
HHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHCCCC
GEEHWNMETATVDFFDMKGDLEAVLELTAKGKAYSFAATKHPALHPGQAAAIMVDGKAIG
CCCCCCCEEEEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEECCCEEE
VIGTVHPELERKFGLNGRTIVFEIEWNAINTRVIPEAAAISKFPANRRDIALVVDGNIAS
EEEECCHHHHHHHCCCCCEEEEEEEECEECCEECCCHHHHHCCCCCCCCEEEEEECCCCC
GDIVEACRVAGGELLKDAKLFDVYVGKGVEEGKKSLAIALTLQSVERTLEEADIAAAVEA
CHHHHHHHHCCHHHHHCCCEEEEEECCCHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHHH
IVQAVSAQFGAALRD
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]