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The map label for this gene is pheS

Identifier: 147673076

GI number: 147673076

Start: 854910

End: 855893

Strand: Direct

Name: pheS

Synonym: VC0395_A0843

Alternate gene names: 147673076

Gene position: 854910-855893 (Clockwise)

Preceding gene: 147675174

Following gene: 147673806

Centisome position: 28.27

GC content: 50.2

Gene sequence:

>984_bases
ATGCAACATCTACAAGAGATTATTGCTAATGCGACCTCAGCGATTAACGCTGCAGAGTCGCTCGTCGCACTGGATGAAGT
GCGTGTTCAGTATCTGGGCAAAAAAGGCGAACTGACGGTTCAACTGCAAAGCCTAGGTAAACTACCGCCGGAAGAGCGCC
GTACTGCTGGCCAAGAAATTAACGTTGCGAAAGAAGCGGTGCAAAAAGCGCTGGCTGAGCGTAAAGATGCGCTGCAAAGC
GCAGAGCTGGAAGCCAAGTTAGCGGCTGAAACCATCGATGTGACACTGCCCGGTCGCCGTATCGAAAATGGTGGTTTGCA
CCCAGTGACTCGTACTATCGAACGTATTGAAAGCTTCTTTGGTGAGCTTGGCTTTACTGTAGAGTCTGGCCCTGAAATCG
AAGATGATTTCCATAACTTCGATGCTCTGAACATTGCCGCGGATCACCCAGCGCGTACTGATCACGATACTTTCTTCTTC
AATCCGAAGTTGATGTTGCGTACTCACACCTCTGGCGTGCAAATCCGTACTCTGGAAGAGCGTCAGCCGCCACTGCGTTT
CATCGCGCCGGGCCGTGTTTACCGTAACGACTACGACCAAACGCATACCCCAATGTTCCACCAAGTGGAAGGTATGTTGG
TTGATGAAAACGTTAACTTTGCACAGCTGAAAGGCATCCTGCATGACTTCTTGTGCAACTTCTTTGAAGAAGATTTGGAA
GTGCGTTTCCGTCCGTCTTACTTCCCGTTCACTGAGCCTTCAGCGGAAGTGGATGTCAAAGGCAAAAACGGCAAATGGCT
AGAAGTGCTAGGCTGCGGCATGGTGCACCCAAATGTACTGCGCAGCGTAGGCATCGACCCTGAGAAATACTCAGGCTTCG
CGTTTGGTATGGGCGTTGAGCGTTTAACCATGCTCCGCTATGGCGTGAATGACCTGCGTGCATTCTTCGAAAACGATCTG
CGTTTCCTAAAACAGTTCAAATAA

Upstream 100 bases:

>100_bases
CCAAGAATCCCTTACGGTAACACTATTGGCGGTCTTGCGACTCCCAAAGAAACCTACTCGGGCTCCTAAAGGAAACTGCT
GATTAGCAGACGAGGAAACG

Downstream 100 bases:

>100_bases
TTCAGGGTTCCCAAGAGGTATATACAATGAAATTCAGCGAATCATGGCTTCGTGAGTGGGTTAACCCGGCAATTACCACT
GACGAATTAACTCACCAAAT

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MQHLQEIIANATSAINAAESLVALDEVRVQYLGKKGELTVQLQSLGKLPPEERRTAGQEINVAKEAVQKALAERKDALQS
AELEAKLAAETIDVTLPGRRIENGGLHPVTRTIERIESFFGELGFTVESGPEIEDDFHNFDALNIAADHPARTDHDTFFF
NPKLMLRTHTSGVQIRTLEERQPPLRFIAPGRVYRNDYDQTHTPMFHQVEGMLVDENVNFAQLKGILHDFLCNFFEEDLE
VRFRPSYFPFTEPSAEVDVKGKNGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLTMLRYGVNDLRAFFENDL
RFLKQFK

Sequences:

>Translated_327_residues
MQHLQEIIANATSAINAAESLVALDEVRVQYLGKKGELTVQLQSLGKLPPEERRTAGQEINVAKEAVQKALAERKDALQS
AELEAKLAAETIDVTLPGRRIENGGLHPVTRTIERIESFFGELGFTVESGPEIEDDFHNFDALNIAADHPARTDHDTFFF
NPKLMLRTHTSGVQIRTLEERQPPLRFIAPGRVYRNDYDQTHTPMFHQVEGMLVDENVNFAQLKGILHDFLCNFFEEDLE
VRFRPSYFPFTEPSAEVDVKGKNGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLTMLRYGVNDLRAFFENDL
RFLKQFK
>Mature_327_residues
MQHLQEIIANATSAINAAESLVALDEVRVQYLGKKGELTVQLQSLGKLPPEERRTAGQEINVAKEAVQKALAERKDALQS
AELEAKLAAETIDVTLPGRRIENGGLHPVTRTIERIESFFGELGFTVESGPEIEDDFHNFDALNIAADHPARTDHDTFFF
NPKLMLRTHTSGVQIRTLEERQPPLRFIAPGRVYRNDYDQTHTPMFHQVEGMLVDENVNFAQLKGILHDFLCNFFEEDLE
VRFRPSYFPFTEPSAEVDVKGKNGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLTMLRYGVNDLRAFFENDL
RFLKQFK

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=278, Percent_Identity=34.1726618705036, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI4758340, Length=259, Percent_Identity=33.976833976834, Blast_Score=137, Evalue=1e-32,
Organism=Escherichia coli, GI1788007, Length=327, Percent_Identity=76.4525993883792, Blast_Score=534, Evalue=1e-153,
Organism=Caenorhabditis elegans, GI32566635, Length=245, Percent_Identity=35.9183673469388, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17508957, Length=260, Percent_Identity=31.9230769230769, Blast_Score=128, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32563657, Length=260, Percent_Identity=31.9230769230769, Blast_Score=128, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6321087, Length=265, Percent_Identity=33.5849056603774, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325304, Length=268, Percent_Identity=27.6119402985075, Blast_Score=105, Evalue=1e-23,
Organism=Drosophila melanogaster, GI18858079, Length=258, Percent_Identity=32.1705426356589, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI17137424, Length=279, Percent_Identity=29.3906810035842, Blast_Score=114, Evalue=7e-26,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_VIBC3 (A5F1W4)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F1W4
- SMR:   A5F1W4
- STRING:   A5F1W4
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A0843
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- BioCyc:   VCHO345073:VC0395_A0843-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 37050; Mature: 37050

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHLQEIIANATSAINAAESLVALDEVRVQYLGKKGELTVQLQSLGKLPPEERRTAGQEI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCHHHHHCCCHH
NVAKEAVQKALAERKDALQSAELEAKLAAETIDVTLPGRRIENGGLHPVTRTIERIESFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
GELGFTVESGPEIEDDFHNFDALNIAADHPARTDHDTFFFNPKLMLRTHTSGVQIRTLEE
HHHCCEECCCCCCCHHHCCCCEEEEECCCCCCCCCCEEEECCEEEEEECCCCEEEEEECC
RQPPLRFIAPGRVYRNDYDQTHTPMFHQVEGMLVDENVNFAQLKGILHDFLCNFFEEDLE
CCCCEEEECCCCHHCCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHC
VRFRPSYFPFTEPSAEVDVKGKNGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVE
EEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHCCCCHHHCCCEEECCCHH
RLTMLRYGVNDLRAFFENDLRFLKQFK
HHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQHLQEIIANATSAINAAESLVALDEVRVQYLGKKGELTVQLQSLGKLPPEERRTAGQEI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCHHHHHCCCHH
NVAKEAVQKALAERKDALQSAELEAKLAAETIDVTLPGRRIENGGLHPVTRTIERIESFF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
GELGFTVESGPEIEDDFHNFDALNIAADHPARTDHDTFFFNPKLMLRTHTSGVQIRTLEE
HHHCCEECCCCCCCHHHCCCCEEEEECCCCCCCCCCEEEECCEEEEEECCCCEEEEEECC
RQPPLRFIAPGRVYRNDYDQTHTPMFHQVEGMLVDENVNFAQLKGILHDFLCNFFEEDLE
CCCCEEEECCCCHHCCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHC
VRFRPSYFPFTEPSAEVDVKGKNGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVE
EEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHCCCCHHHCCCEEECCCHH
RLTMLRYGVNDLRAFFENDLRFLKQFK
HHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA