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The map label for this gene is rnhB

Identifier: 147673627

GI number: 147673627

Start: 1954593

End: 1955213

Strand: Reverse

Name: rnhB

Synonym: VC0395_A1837

Alternate gene names: 147673627

Gene position: 1955213-1954593 (Counterclockwise)

Preceding gene: 147673997

Following gene: 147675187

Centisome position: 64.66

GC content: 51.53

Gene sequence:

>621_bases
ATGGCGAAGAAACCAAGCATTGAGCTGCCTCCCTTTGAGATTCCTGCAGGTTATGCCTTGATTGCTGGAGTGGATGAAGT
CGGGCGCGGCCCACTGGTGGGGGATGTCGTCACGGCAGCAGTGATCCTTGACCCTAATCGGCCGATTATGGGCTTGAATG
ATTCCAAAAAGCTCAGTGAGAAAAAACGCTTAGCCCTGTTTCCTGAGATTCAGGTAAAAGCCTTAGCTTGGGCGGTGGGG
CGCTGTTCGCCGCAAGAAATTGATGAGCTGAACATCTTCCAAGCCACCATGGTCGCAATGCAGCGCGCCGTCGCAGGGTT
GCGCATTCAGCCTGATTTGGTATTGATTGATGGCAATAAAATTCCCAAGCTCCCAATGGAGGCGCAAGCCGTGGTTAAAG
GCGATCTGCGTGTCGCGCAAATCAGTGCCGCTTCGATTATTGCCAAAGTGATCCGCGACCAAGAGATGGAAGCGTTGGAT
AAACAATATCCTCAGTTTGGTTTCGCCAAGCACAAAGGTTACCCAACCGCCGCGCATTTTGCTGCCATTGAGCAGCATGG
AGTGATTGAGCAGCACCGTAAAAGTTTTGGCCCAGTAAAACGTGCTCTTGGTATAGAATAG

Upstream 100 bases:

>100_bases
CGAAATGCATCAATGGATCCGTAAAGATGCGGATCAGCAAGCGGCGCAAGCTGTGCTGCATTTGATTCAGAAATAACATT
AACCAGATTGAGAAGATACC

Downstream 100 bases:

>100_bases
AGCGCAATTGCGTTTCCTAAGATTTGGATACTCCTTGCATGTCAGACCCAAAGTTCATCCACTTACGTATACACAGTGAT
TTCTCTATGGTGGATGGTTT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MAKKPSIELPPFEIPAGYALIAGVDEVGRGPLVGDVVTAAVILDPNRPIMGLNDSKKLSEKKRLALFPEIQVKALAWAVG
RCSPQEIDELNIFQATMVAMQRAVAGLRIQPDLVLIDGNKIPKLPMEAQAVVKGDLRVAQISAASIIAKVIRDQEMEALD
KQYPQFGFAKHKGYPTAAHFAAIEQHGVIEQHRKSFGPVKRALGIE

Sequences:

>Translated_206_residues
MAKKPSIELPPFEIPAGYALIAGVDEVGRGPLVGDVVTAAVILDPNRPIMGLNDSKKLSEKKRLALFPEIQVKALAWAVG
RCSPQEIDELNIFQATMVAMQRAVAGLRIQPDLVLIDGNKIPKLPMEAQAVVKGDLRVAQISAASIIAKVIRDQEMEALD
KQYPQFGFAKHKGYPTAAHFAAIEQHGVIEQHRKSFGPVKRALGIE
>Mature_205_residues
AKKPSIELPPFEIPAGYALIAGVDEVGRGPLVGDVVTAAVILDPNRPIMGLNDSKKLSEKKRLALFPEIQVKALAWAVGR
CSPQEIDELNIFQATMVAMQRAVAGLRIQPDLVLIDGNKIPKLPMEAQAVVKGDLRVAQISAASIIAKVIRDQEMEALDK
QYPQFGFAKHKGYPTAAHFAAIEQHGVIEQHRKSFGPVKRALGIE

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=186, Percent_Identity=73.1182795698925, Blast_Score=281, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_VIBC3 (A5F626)

Other databases:

- EMBL:   CP000627
- EMBL:   CP001235
- ProteinModelPortal:   A5F626
- SMR:   A5F626
- STRING:   A5F626
- GenomeReviews:   CP000627_GR
- GenomeReviews:   CP001235_GR
- KEGG:   vco:VC0395_A1837
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   VCHO345073:VC0395_A1837-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 22390; Mature: 22258

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKPSIELPPFEIPAGYALIAGVDEVGRGPLVGDVVTAAVILDPNRPIMGLNDSKKLSE
CCCCCCCCCCCCCCCCCEEEEECHHHHCCCCCHHHHHEEEEEECCCCCEECCCCCHHHHH
KKRLALFPEIQVKALAWAVGRCSPQEIDELNIFQATMVAMQRAVAGLRIQPDLVLIDGNK
HHHEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCCEEEECCCC
IPKLPMEAQAVVKGDLRVAQISAASIIAKVIRDQEMEALDKQYPQFGFAKHKGYPTAAHF
CCCCCCHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHH
AAIEQHGVIEQHRKSFGPVKRALGIE
HHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKKPSIELPPFEIPAGYALIAGVDEVGRGPLVGDVVTAAVILDPNRPIMGLNDSKKLSE
CCCCCCCCCCCCCCCCEEEEECHHHHCCCCCHHHHHEEEEEECCCCCEECCCCCHHHHH
KKRLALFPEIQVKALAWAVGRCSPQEIDELNIFQATMVAMQRAVAGLRIQPDLVLIDGNK
HHHEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCCEEEECCCC
IPKLPMEAQAVVKGDLRVAQISAASIIAKVIRDQEMEALDKQYPQFGFAKHKGYPTAAHF
CCCCCCHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHH
AAIEQHGVIEQHRKSFGPVKRALGIE
HHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA