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The map label for this gene is pgk [H]

Identifier: 147673541

GI number: 147673541

Start: 26190

End: 27368

Strand: Direct

Name: pgk [H]

Synonym: VC0395_A0030

Alternate gene names: 147673541

Gene position: 26190-27368 (Clockwise)

Preceding gene: 229259769

Following gene: 147674645

Centisome position: 0.87

GC content: 49.11

Gene sequence:

>1179_bases
TTGAGAGGACAAATCATGTCTGTAATCAAGATGATTGACCTGGATCTTGCAGGTAAACGCGTATTTATCCGTGCTGACCT
GAACGTACCAGTAAAAGACGGCAAAGTGACTTCTGATGCACGTATCCTTGCATCACTACCTACCATTAAGCACTGCTTGG
AAGCGGGCGCGAAAGTAATGGTCACTTCTCACCTAGGTCGTCCAACGGAAGGTGAGTACGCTGAAGAGTTCTCTCTACTG
CCTGTGGTTAACTACCTGAACGATGCTCTCGATTGCGAAGTTCGCCTAGTGAAAGATTACCTAGACGGCGTTGAACTGAA
CGCAGGTGAACTGGTTGTTCTGGAAAACGTTCGCTTCAACAAAGGCGAAAAGAAAAACGAAGAAGCGCTGTCTAAGAAAT
ACGCGGCACTGTGTGACGTATTCGTGATGGATGCGTTTGGTACTGCGCACCGTGCTCAAGCTTCTACTCACGGTGTTGGT
ATGTTTGCTCCTATCGCGTGTGCAGGCCCTCTACTGGCTGACGAACTTGAAGCGCTAGGCAAAGCGATGGACAAACCAGC
TCGCCCAATGGTAGCTATCGTTGGTGGTTCAAAAGTATCAACTAAGCTGACTGTACTTGAGTCACTGTCAAAAATTGCTG
ACCAACTGGTGGTTGGCGGTGGTATCGCGAACACTTTCATCGCTGCAGCGGGTCACAACGTAGGTAAGTCTCTGTATGAA
GCTGACCTTGTTGAAACAGCGAAAAAACTGATGGAAGAGTGCGCGATTCCTGTGGCGACTGACGTAGCTTGTGCCAAAGC
TTTCGATGAGAACGCAGAAGCCGAAATCAAACACGTTTCTGAAGTTCAAGATGACGACATGATTTTCGACTTGGGTCCAA
ACTCGACGGCTGAACTGGCTGAGATCCTGAAAAACGCGAAAACTATCCTGTGGAACGGCCCAGTAGGCGTATTCGAATTC
AAGAACTTTGAAGCGGGTACTCGTGGTATCGCAGAAGCGATCGCACAATCAGAAGGCTTCTCTGTTGCAGGTGGCGGTGA
CACGCTAGCGGCGATCGACAAGTTTGGCATCAAAGCCGATGTTTCTTACATCTCTACTGGTGGCGGCGCGTTCCTTGAGT
TCGTTGAAGGCAAAAAACTGCCTGCAGTAGAAATGCTGGAAGCACGCGCTAAAGCGTAA

Upstream 100 bases:

>100_bases
ATTGAAACCCGAAGGACGCATCCCCATATCGGTGAACAGCTAAGATTTTACCGACTCGGCAGGGTCGCCGAGTTGCAGAA
TTTTCATTTCTTTAAATAGT

Downstream 100 bases:

>100_bases
TAATCGAATGAGAGCGGGGGGAAGACTCCCGCTTTCTTGTTTGCTGCACGGCGAGCATTTGTTTGGTAGAATGCCGCGAT
TGGTGAGCAAACGTTTGCAA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MRGQIMSVIKMIDLDLAGKRVFIRADLNVPVKDGKVTSDARILASLPTIKHCLEAGAKVMVTSHLGRPTEGEYAEEFSLL
PVVNYLNDALDCEVRLVKDYLDGVELNAGELVVLENVRFNKGEKKNEEALSKKYAALCDVFVMDAFGTAHRAQASTHGVG
MFAPIACAGPLLADELEALGKAMDKPARPMVAIVGGSKVSTKLTVLESLSKIADQLVVGGGIANTFIAAAGHNVGKSLYE
ADLVETAKKLMEECAIPVATDVACAKAFDENAEAEIKHVSEVQDDDMIFDLGPNSTAELAEILKNAKTILWNGPVGVFEF
KNFEAGTRGIAEAIAQSEGFSVAGGGDTLAAIDKFGIKADVSYISTGGGAFLEFVEGKKLPAVEMLEARAKA

Sequences:

>Translated_392_residues
MRGQIMSVIKMIDLDLAGKRVFIRADLNVPVKDGKVTSDARILASLPTIKHCLEAGAKVMVTSHLGRPTEGEYAEEFSLL
PVVNYLNDALDCEVRLVKDYLDGVELNAGELVVLENVRFNKGEKKNEEALSKKYAALCDVFVMDAFGTAHRAQASTHGVG
MFAPIACAGPLLADELEALGKAMDKPARPMVAIVGGSKVSTKLTVLESLSKIADQLVVGGGIANTFIAAAGHNVGKSLYE
ADLVETAKKLMEECAIPVATDVACAKAFDENAEAEIKHVSEVQDDDMIFDLGPNSTAELAEILKNAKTILWNGPVGVFEF
KNFEAGTRGIAEAIAQSEGFSVAGGGDTLAAIDKFGIKADVSYISTGGGAFLEFVEGKKLPAVEMLEARAKA
>Mature_392_residues
MRGQIMSVIKMIDLDLAGKRVFIRADLNVPVKDGKVTSDARILASLPTIKHCLEAGAKVMVTSHLGRPTEGEYAEEFSLL
PVVNYLNDALDCEVRLVKDYLDGVELNAGELVVLENVRFNKGEKKNEEALSKKYAALCDVFVMDAFGTAHRAQASTHGVG
MFAPIACAGPLLADELEALGKAMDKPARPMVAIVGGSKVSTKLTVLESLSKIADQLVVGGGIANTFIAAAGHNVGKSLYE
ADLVETAKKLMEECAIPVATDVACAKAFDENAEAEIKHVSEVQDDDMIFDLGPNSTAELAEILKNAKTILWNGPVGVFEF
KNFEAGTRGIAEAIAQSEGFSVAGGGDTLAAIDKFGIKADVSYISTGGGAFLEFVEGKKLPAVEMLEARAKA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=406, Percent_Identity=38.423645320197, Blast_Score=250, Evalue=1e-66,
Organism=Homo sapiens, GI31543397, Length=406, Percent_Identity=37.9310344827586, Blast_Score=250, Evalue=1e-66,
Organism=Escherichia coli, GI1789294, Length=386, Percent_Identity=78.7564766839378, Blast_Score=605, Evalue=1e-174,
Organism=Caenorhabditis elegans, GI17508823, Length=408, Percent_Identity=40.1960784313725, Blast_Score=264, Evalue=6e-71,
Organism=Saccharomyces cerevisiae, GI6319857, Length=420, Percent_Identity=38.5714285714286, Blast_Score=256, Evalue=6e-69,
Organism=Drosophila melanogaster, GI17136394, Length=411, Percent_Identity=40.1459854014599, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24581203, Length=420, Percent_Identity=37.6190476190476, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 41565; Mature: 41565

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGQIMSVIKMIDLDLAGKRVFIRADLNVPVKDGKVTSDARILASLPTIKHCLEAGAKVM
CCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEE
VTSHLGRPTEGEYAEEFSLLPVVNYLNDALDCEVRLVKDYLDGVELNAGELVVLENVRFN
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCC
KGEKKNEEALSKKYAALCDVFVMDAFGTAHRAQASTHGVGMFAPIACAGPLLADELEALG
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHH
KAMDKPARPMVAIVGGSKVSTKLTVLESLSKIADQLVVGGGIANTFIAAAGHNVGKSLYE
HHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHH
ADLVETAKKLMEECAIPVATDVACAKAFDENAEAEIKHVSEVQDDDMIFDLGPNSTAELA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHH
EILKNAKTILWNGPVGVFEFKNFEAGTRGIAEAIAQSEGFSVAGGGDTLAAIDKFGIKAD
HHHHCCCEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHCCCEEE
VSYISTGGGAFLEFVEGKKLPAVEMLEARAKA
HHEEECCCHHHHHHHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MRGQIMSVIKMIDLDLAGKRVFIRADLNVPVKDGKVTSDARILASLPTIKHCLEAGAKVM
CCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEE
VTSHLGRPTEGEYAEEFSLLPVVNYLNDALDCEVRLVKDYLDGVELNAGELVVLENVRFN
EEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCC
KGEKKNEEALSKKYAALCDVFVMDAFGTAHRAQASTHGVGMFAPIACAGPLLADELEALG
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHH
KAMDKPARPMVAIVGGSKVSTKLTVLESLSKIADQLVVGGGIANTFIAAAGHNVGKSLYE
HHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHH
ADLVETAKKLMEECAIPVATDVACAKAFDENAEAEIKHVSEVQDDDMIFDLGPNSTAELA
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHH
EILKNAKTILWNGPVGVFEFKNFEAGTRGIAEAIAQSEGFSVAGGGDTLAAIDKFGIKAD
HHHHCCCEEEECCCCCEEEECCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHHHCCCEEE
VSYISTGGGAFLEFVEGKKLPAVEMLEARAKA
HHEEECCCHHHHHHHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA