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The map label for this gene is mexA [H]

Identifier: 147673434

GI number: 147673434

Start: 1362415

End: 1363446

Strand: Reverse

Name: mexA [H]

Synonym: VC0395_A1280

Alternate gene names: 147673434

Gene position: 1363446-1362415 (Counterclockwise)

Preceding gene: 147673938

Following gene: 147675113

Centisome position: 45.09

GC content: 50.78

Gene sequence:

>1032_bases
ATGAACAAAACCCTTTTGGCCTTTTCCATCTGCTCCACTTTGCTGGTGGGGTGTGGAAAAGAGCTTCCTCCCGTGCCTGA
GCCCGATTCGCGGCCAGCTAAATTGACGACTGTGTCCGTAGGTAACAACACCTTTTCTCGCCAATTTCCAGCCACTACGG
CGGCTGGCGATCGCGCGGTGCTCGCTTTCCGTGTACCCGGACAACTACAAACCATTGATGTTTTAGCCGGACAAGAGGTG
AAGAAAGGTGAGGTACTTGCCAGACTCAACCCTGACGAATATGCGTTGTTAGAACAGCAAGCCAACGCCAATTTTCAATT
AGCCGATGTGCAATTTCAGCGGGCACAGCGATTACGCCAAGACAAAGTCGTGTCCGAGCAGGATTTCGACCAAGCTCAAG
CCAATCACAATTCCGCGAAAGCCACCTGGGAACAAGCCAAAGCCAATCTGCGTTATACCCAATTGATCGCCCCTTATGAT
GGCACCATTTCGCTGATCCCAGCAGAGCAGTATGAGTATGTCGCAGCCAAGCAAGGGGTGATGAATATTCAAACCAATCA
ACTGCTCAAAGTGCAGTTTCTCCTTCCCGATCATTTGCTTAATCGCTTTTCTCGTGACGGTGTAGAAGCGCATATGGTGT
TTGACTCGTTCCCTAACCGCCAATATCCGCTGCAATTTCAAGAGATAGATACCGAGGCCGATTCCAAAACCCGCTCCTAT
AAAGTGACTATGGTGATGGAAAGACCCAATGATGTCGGCATTTTGCCGGGTATGGCGGGACGTGTAAGCCTCATGGCTCC
GAGTGGTAGCGCGACGGTGATCCCCACTTCTGCATTGTTTGCAGAGCAAGGGGGAAAATACGTATGGCGAGTCGATGAAG
CGGGGCTGGTCAGTAAAGCTGCGGTCACTTTGAATGATCAACGCCAAGTGCTTACAGGTTTAAATGATGGTGACCGGATC
ATTATTTCCGGTGTCAGTGGTGTGACCGAGGGCATAAAAGTGCGTGAATGGGTCAAGGAGCGAGGACTGTAG

Upstream 100 bases:

>100_bases
TCTCACTTTCGCTAATATTTGGTTAAAAATCGATGGCGCGAGTGGAGTTAACGCACTGATTCACCTATGTTTGAGCTATG
TCTAAGCATAGGTAATTGGT

Downstream 100 bases:

>100_bases
GATGAAAATAATTCGTTACACCGCTTGGTTGGCTCACTTACTGTTGATCGCAGGATGCAGTGAACCCACGAAGCTTGAAC
GCGATACGCTACCGCGAGTA

Product: putative multidrug resistance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MNKTLLAFSICSTLLVGCGKELPPVPEPDSRPAKLTTVSVGNNTFSRQFPATTAAGDRAVLAFRVPGQLQTIDVLAGQEV
KKGEVLARLNPDEYALLEQQANANFQLADVQFQRAQRLRQDKVVSEQDFDQAQANHNSAKATWEQAKANLRYTQLIAPYD
GTISLIPAEQYEYVAAKQGVMNIQTNQLLKVQFLLPDHLLNRFSRDGVEAHMVFDSFPNRQYPLQFQEIDTEADSKTRSY
KVTMVMERPNDVGILPGMAGRVSLMAPSGSATVIPTSALFAEQGGKYVWRVDEAGLVSKAAVTLNDQRQVLTGLNDGDRI
IISGVSGVTEGIKVREWVKERGL

Sequences:

>Translated_343_residues
MNKTLLAFSICSTLLVGCGKELPPVPEPDSRPAKLTTVSVGNNTFSRQFPATTAAGDRAVLAFRVPGQLQTIDVLAGQEV
KKGEVLARLNPDEYALLEQQANANFQLADVQFQRAQRLRQDKVVSEQDFDQAQANHNSAKATWEQAKANLRYTQLIAPYD
GTISLIPAEQYEYVAAKQGVMNIQTNQLLKVQFLLPDHLLNRFSRDGVEAHMVFDSFPNRQYPLQFQEIDTEADSKTRSY
KVTMVMERPNDVGILPGMAGRVSLMAPSGSATVIPTSALFAEQGGKYVWRVDEAGLVSKAAVTLNDQRQVLTGLNDGDRI
IISGVSGVTEGIKVREWVKERGL
>Mature_343_residues
MNKTLLAFSICSTLLVGCGKELPPVPEPDSRPAKLTTVSVGNNTFSRQFPATTAAGDRAVLAFRVPGQLQTIDVLAGQEV
KKGEVLARLNPDEYALLEQQANANFQLADVQFQRAQRLRQDKVVSEQDFDQAQANHNSAKATWEQAKANLRYTQLIAPYD
GTISLIPAEQYEYVAAKQGVMNIQTNQLLKVQFLLPDHLLNRFSRDGVEAHMVFDSFPNRQYPLQFQEIDTEADSKTRSY
KVTMVMERPNDVGILPGMAGRVSLMAPSGSATVIPTSALFAEQGGKYVWRVDEAGLVSKAAVTLNDQRQVLTGLNDGDRI
IISGVSGVTEGIKVREWVKERGL

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=313, Percent_Identity=25.5591054313099, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI87081796, Length=301, Percent_Identity=29.9003322259136, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789929, Length=388, Percent_Identity=25, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 37766; Mature: 37766

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTLLAFSICSTLLVGCGKELPPVPEPDSRPAKLTTVSVGNNTFSRQFPATTAAGDRAV
CCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEE
LAFRVPGQLQTIDVLAGQEVKKGEVLARLNPDEYALLEQQANANFQLADVQFQRAQRLRQ
EEEECCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHCCCCEEEEHHHHHHHHHHHH
DKVVSEQDFDQAQANHNSAKATWEQAKANLRYTQLIAPYDGTISLIPAEQYEYVAAKQGV
HCCCCCCCHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCEEEEEECHHHHHHHHHCCC
MNIQTNQLLKVQFLLPDHLLNRFSRDGVEAHMVFDSFPNRQYPLQFQEIDTEADSKTRSY
EEECCCCEEEEEEECCHHHHHHHCCCCCEEEEEEECCCCCCCCEEHEECCCCCCCCCCEE
KVTMVMERPNDVGILPGMAGRVSLMAPSGSATVIPTSALFAEQGGKYVWRVDEAGLVSKA
EEEEEEECCCCCEECCCCCCEEEEECCCCCCEEEECHHHHHHCCCCEEEEECCCCCEEEE
AVTLNDQRQVLTGLNDGDRIIISGVSGVTEGIKVREWVKERGL
EEEECCCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKTLLAFSICSTLLVGCGKELPPVPEPDSRPAKLTTVSVGNNTFSRQFPATTAAGDRAV
CCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEE
LAFRVPGQLQTIDVLAGQEVKKGEVLARLNPDEYALLEQQANANFQLADVQFQRAQRLRQ
EEEECCCCEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHCCCCEEEEHHHHHHHHHHHH
DKVVSEQDFDQAQANHNSAKATWEQAKANLRYTQLIAPYDGTISLIPAEQYEYVAAKQGV
HCCCCCCCHHHHHCCCCCHHHHHHHHHCCCEEEEEEECCCCEEEEEECHHHHHHHHHCCC
MNIQTNQLLKVQFLLPDHLLNRFSRDGVEAHMVFDSFPNRQYPLQFQEIDTEADSKTRSY
EEECCCCEEEEEEECCHHHHHHHCCCCCEEEEEEECCCCCCCCEEHEECCCCCCCCCCEE
KVTMVMERPNDVGILPGMAGRVSLMAPSGSATVIPTSALFAEQGGKYVWRVDEAGLVSKA
EEEEEEECCCCCEECCCCCCEEEEECCCCCCEEEECHHHHHHCCCCEEEEECCCCCEEEE
AVTLNDQRQVLTGLNDGDRIIISGVSGVTEGIKVREWVKERGL
EEEECCCHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]