Click here to switch to the map view.

The map label for this gene is ampD [H]

Identifier: 147673296

GI number: 147673296

Start: 2148400

End: 2148945

Strand: Reverse

Name: ampD [H]

Synonym: VC0395_A1997

Alternate gene names: 147673296

Gene position: 2148945-2148400 (Counterclockwise)

Preceding gene: 147675576

Following gene: 147673043

Centisome position: 71.06

GC content: 53.85

Gene sequence:

>546_bases
ATGATTGATAGTCAAGGCTGGTACCGCCTCGCCAGAAAAGTACCTTCTCCACATTGTGATGTGCGCCATGATAGTGAGGA
TATTTCGCTACTTGTGGTGCACAACATCAGCTTGCCCCCCGGCCAGTTTGGCGGCCCGTACATTGAGCAGTTTTTTCTGG
GCGAGCTGGATGCCAATCAACATTCGTTTTTTAAGGTAATCGCCAAGATGCGCGTGTCGGCGCACTGCGTGATCCGCCGA
GATGGTGAGGTGGTGCAGTTTGTGCCGTTTCATCTGCGCGCGTGGCATGCGGGTGTTTCCTCATTTGCAGGCCGAGCCAA
GTGCAATGATTACTCGATTGGTATTGAGCTGGAGGGGAGTGATTTTGTGCCGTATACCGATGCGCAATATCAGGCGCTGA
CTGAGCTGACCCAAGCCTTGATGGTGCGCTACCCGCACATCACGTTGCCGCGCATTACTGGACATCAATATATTGCGCCG
CTGCGTAAAACCGACCCCGGTTTGGTGTTTGACTGGCGGCGTTTTCGGGCTGGGTTGAAGTCATAG

Upstream 100 bases:

>100_bases
TGCTCACGGGGACTTACTTGCCGCCTAGCTGCAACTCCAAGTCGTTTGGGGATAGGCTTGGCGTTACTCTCAACCGACAA
GATATAAGCAAGGAGTAAGG

Downstream 100 bases:

>100_bases
GTTTTAGTTGGTGCGAAATACCCCTCCCTGCCTCCCCCTAGAAGAGGGAGGGGTAAGGGCGGTGCTTGGTTTTGAGTTCC
CTCCCCTTTTAGGGGAGGGG

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MIDSQGWYRLARKVPSPHCDVRHDSEDISLLVVHNISLPPGQFGGPYIEQFFLGELDANQHSFFKVIAKMRVSAHCVIRR
DGEVVQFVPFHLRAWHAGVSSFAGRAKCNDYSIGIELEGSDFVPYTDAQYQALTELTQALMVRYPHITLPRITGHQYIAP
LRKTDPGLVFDWRRFRAGLKS

Sequences:

>Translated_181_residues
MIDSQGWYRLARKVPSPHCDVRHDSEDISLLVVHNISLPPGQFGGPYIEQFFLGELDANQHSFFKVIAKMRVSAHCVIRR
DGEVVQFVPFHLRAWHAGVSSFAGRAKCNDYSIGIELEGSDFVPYTDAQYQALTELTQALMVRYPHITLPRITGHQYIAP
LRKTDPGLVFDWRRFRAGLKS
>Mature_181_residues
MIDSQGWYRLARKVPSPHCDVRHDSEDISLLVVHNISLPPGQFGGPYIEQFFLGELDANQHSFFKVIAKMRVSAHCVIRR
DGEVVQFVPFHLRAWHAGVSSFAGRAKCNDYSIGIELEGSDFVPYTDAQYQALTELTQALMVRYPHITLPRITGHQYIAP
LRKTDPGLVFDWRRFRAGLKS

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=176, Percent_Identity=63.0681818181818, Blast_Score=230, Evalue=5e-62,
Organism=Escherichia coli, GI1787092, Length=130, Percent_Identity=36.9230769230769, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 20612; Mature: 20612

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDSQGWYRLARKVPSPHCDVRHDSEDISLLVVHNISLPPGQFGGPYIEQFFLGELDANQ
CCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCH
HSFFKVIAKMRVSAHCVIRRDGEVVQFVPFHLRAWHAGVSSFAGRAKCNDYSIGIELEGS
HHHHHHHHHHHHCEEEEEECCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCC
DFVPYTDAQYQALTELTQALMVRYPHITLPRITGHQYIAPLRKTDPGLVFDWRRFRAGLK
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCHHCCCCCCCCCCEEEEHHHHHCCCC
S
C
>Mature Secondary Structure
MIDSQGWYRLARKVPSPHCDVRHDSEDISLLVVHNISLPPGQFGGPYIEQFFLGELDANQ
CCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCH
HSFFKVIAKMRVSAHCVIRRDGEVVQFVPFHLRAWHAGVSSFAGRAKCNDYSIGIELEGS
HHHHHHHHHHHHCEEEEEECCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCC
DFVPYTDAQYQALTELTQALMVRYPHITLPRITGHQYIAPLRKTDPGLVFDWRRFRAGLK
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEECCCHHCCCCCCCCCCEEEEHHHHHCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383940 [H]