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The map label for this gene is xerD

Identifier: 147673043

GI number: 147673043

Start: 2146613

End: 2147521

Strand: Reverse

Name: xerD

Synonym: VC0395_A1995

Alternate gene names: 147673043

Gene position: 2147521-2146613 (Counterclockwise)

Preceding gene: 147673296

Following gene: 147674865

Centisome position: 71.01

GC content: 50.5

Gene sequence:

>909_bases
GTGAGCGAAGCCTTATCTCCCGACCAAGGGTTGGTGGAACAATTTTTAGATACCATGTGGTTTGAGCGCGGTTTGGCAGA
AAATACCGTGGCGTCTTATCGTAATGACTTGAGTAAGCTATTAGAGTGGATGGCGCAGAACCAGTATCGGCTCGATTTTA
TTAGCTTTGCTGGTTTGCAGGAGTATCAAAGCTGGCTGTCGGAGCAAAATTACAAACCGACCTCGAAAGCGCGCATGTTG
TCGGCGATTCGTCGCCTGTTTCAATATCTGCATCGGGAAAAGGTGCGCGCTGATGATCCGAGTGCGCTGTTGGTGAGCCC
GAAACTGCCAACGCGCTTACCAAAAGATTTGAGCGAAGCGCAAGTTGAAGCTTTGCTGAGTGCGCCAGATCCGCAAAGTC
CACTTGAGCTGCGTGATAAAGCCATGCTGGAATTGCTGTATGCTACGGGACTGCGTGTGACAGAGCTGGTCTCACTGACC
ATGGAAAACATGAGCTTGCGCCAAGGTGTGGTGCGAGTCATGGGTAAGGGTGGTAAAGAGCGTTTGGTGCCTATGGGTGA
AAATGCCATTGAATGGATTGAAACTTTTTTGCAGCAGGGACGGTCTTTACTGCTAGGGGAGCAGACGTCGGATATCGTTT
TCCCCAGTAGCCGCGGTCAGCAGATGACCCGGCAAACCTTCTGGCATCGCATTAAACACTATGCGGTTATTGCAGGAATT
GATGTGGAAAAACTCTCGCCGCACGTATTGCGCCATGCGTTTGCCACTCACTTGCTCAATTATGGGGCCGATCTGCGCGT
AGTGCAGATGTTGCTTGGGCATAGTGATTTATCGACCACACAAATTTATACTCATGTCGCCACTGAGCGGCTTAAACAAC
TCCACAACGAGCACCATCCAAGGGCTTGA

Upstream 100 bases:

>100_bases
CTAACCATAAAAATGATCGGAATGCAGCCAATATCCTGCATCCTGCGTATTTTTATGCTATTAAACGCCCAGTAAAAAGA
GAGCTAGAGCTGAGGATAGA

Downstream 100 bases:

>100_bases
AAATAAGGTGATTTTCATGAGCGTACTGCGCCGACTAACATGGCTGGCATTTCCTTTACTGAGTATGGCATTCAACGTAC
AAGCGAACACTGCACCAGCA

Product: site-specific tyrosine recombinase XerD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MSEALSPDQGLVEQFLDTMWFERGLAENTVASYRNDLSKLLEWMAQNQYRLDFISFAGLQEYQSWLSEQNYKPTSKARML
SAIRRLFQYLHREKVRADDPSALLVSPKLPTRLPKDLSEAQVEALLSAPDPQSPLELRDKAMLELLYATGLRVTELVSLT
MENMSLRQGVVRVMGKGGKERLVPMGENAIEWIETFLQQGRSLLLGEQTSDIVFPSSRGQQMTRQTFWHRIKHYAVIAGI
DVEKLSPHVLRHAFATHLLNYGADLRVVQMLLGHSDLSTTQIYTHVATERLKQLHNEHHPRA

Sequences:

>Translated_302_residues
MSEALSPDQGLVEQFLDTMWFERGLAENTVASYRNDLSKLLEWMAQNQYRLDFISFAGLQEYQSWLSEQNYKPTSKARML
SAIRRLFQYLHREKVRADDPSALLVSPKLPTRLPKDLSEAQVEALLSAPDPQSPLELRDKAMLELLYATGLRVTELVSLT
MENMSLRQGVVRVMGKGGKERLVPMGENAIEWIETFLQQGRSLLLGEQTSDIVFPSSRGQQMTRQTFWHRIKHYAVIAGI
DVEKLSPHVLRHAFATHLLNYGADLRVVQMLLGHSDLSTTQIYTHVATERLKQLHNEHHPRA
>Mature_301_residues
SEALSPDQGLVEQFLDTMWFERGLAENTVASYRNDLSKLLEWMAQNQYRLDFISFAGLQEYQSWLSEQNYKPTSKARMLS
AIRRLFQYLHREKVRADDPSALLVSPKLPTRLPKDLSEAQVEALLSAPDPQSPLELRDKAMLELLYATGLRVTELVSLTM
ENMSLRQGVVRVMGKGGKERLVPMGENAIEWIETFLQQGRSLLLGEQTSDIVFPSSRGQQMTRQTFWHRIKHYAVIAGID
VEKLSPHVLRHAFATHLLNYGADLRVVQMLLGHSDLSTTQIYTHVATERLKQLHNEHHPRA

Specific function: Site-specific tyrosine recombinase, which acts by catalyzing the cutting and rejoining of the recombining DNA molecules. The xerC-xerD complex is essential to convert dimers of the bacterial chromosome into monomers to permit their segregation at cell div

COG id: COG4974

COG function: function code L; Site-specific recombinase XerD

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family. XerD subfamily

Homologues:

Organism=Escherichia coli, GI1789261, Length=295, Percent_Identity=70.1694915254237, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI1790244, Length=302, Percent_Identity=36.4238410596026, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1790768, Length=179, Percent_Identity=30.1675977653631, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790767, Length=169, Percent_Identity=28.9940828402367, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): XERD_VIBCH (Q9KPE9)

Other databases:

- EMBL:   AE003852
- PIR:   A82080
- RefSeq:   NP_232049.1
- ProteinModelPortal:   Q9KPE9
- SMR:   Q9KPE9
- GeneID:   2613088
- GenomeReviews:   AE003852_GR
- KEGG:   vch:VC2419
- TIGR:   VC_2419
- HOGENOM:   HBG727654
- OMA:   YLEYGRP
- ProtClustDB:   PRK00283
- GO:   GO:0005737
- HAMAP:   MF_01807
- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR010998
- InterPro:   IPR023109
- InterPro:   IPR004107
- InterPro:   IPR011932
- Gene3D:   G3DSA:1.10.150.130
- Gene3D:   G3DSA:1.10.443.10
- TIGRFAMs:   TIGR02225

Pfam domain/function: PF02899 Phage_integr_N; PF00589 Phage_integrase; SSF56349 DNA_brk_join_enz; SSF47823 L_intgrse_like_N

EC number: NA

Molecular weight: Translated: 34565; Mature: 34434

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: ACT_SITE 152-152 ACT_SITE 176-176 ACT_SITE 248-248 ACT_SITE 251-251 ACT_SITE 274-274 ACT_SITE 283-283

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEALSPDQGLVEQFLDTMWFERGLAENTVASYRNDLSKLLEWMAQNQYRLDFISFAGLQ
CCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EYQSWLSEQNYKPTSKARMLSAIRRLFQYLHREKVRADDPSALLVSPKLPTRLPKDLSEA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHH
QVEALLSAPDPQSPLELRDKAMLELLYATGLRVTELVSLTMENMSLRQGVVRVMGKGGKE
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RLVPMGENAIEWIETFLQQGRSLLLGEQTSDIVFPSSRGQQMTRQTFWHRIKHYAVIAGI
CCCCCCCHHHHHHHHHHHCCCCEECCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCC
DVEKLSPHVLRHAFATHLLNYGADLRVVQMLLGHSDLSTTQIYTHVATERLKQLHNEHHP
CHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
RA
CC
>Mature Secondary Structure 
SEALSPDQGLVEQFLDTMWFERGLAENTVASYRNDLSKLLEWMAQNQYRLDFISFAGLQ
CCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
EYQSWLSEQNYKPTSKARMLSAIRRLFQYLHREKVRADDPSALLVSPKLPTRLPKDLSEA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHH
QVEALLSAPDPQSPLELRDKAMLELLYATGLRVTELVSLTMENMSLRQGVVRVMGKGGKE
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
RLVPMGENAIEWIETFLQQGRSLLLGEQTSDIVFPSSRGQQMTRQTFWHRIKHYAVIAGI
CCCCCCCHHHHHHHHHHHCCCCEECCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHCCC
DVEKLSPHVLRHAFATHLLNYGADLRVVQMLLGHSDLSTTQIYTHVATERLKQLHNEHHP
CHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCC
RA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301; 12050668