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The map label for this gene is ybfF [C]

Identifier: 146343448

GI number: 146343448

Start: 6939847

End: 6940809

Strand: Direct

Name: ybfF [C]

Synonym: BRADO6677

Alternate gene names: 146343448

Gene position: 6939847-6940809 (Clockwise)

Preceding gene: 146343446

Following gene: 146343449

Centisome position: 93.07

GC content: 70.3

Gene sequence:

>963_bases
ATGACCCTGCCCCGCCTGCTGCAACGCACCGCCCTCGCCGCCACCGCGCTCACCGCCCTTGCCGGCGCCGCCCTCGCGGA
CGCCGAGGAAGAGCCCGGCCATTTCCCGCCGGCCTTCCGCACGCAGGAGATCCCCACCAACGGCACCACGATCCACGTCC
GCGTCGGCGGCACCGGCCCTGCAGTGGTGCTGCTGCACGGCTATGGCGACACCGGCGACATGTGGGTGCCGCTCGCCGCC
AAGCTGGCCGCAAACCACACCGTGATCGTGCCCGATCTGCGCGGGCTCGGCCTGTCCGCCCGCGCCGACAAGGGTTTTGA
AAAGGCTAACCAGGCCGAGGACATCACCGGCGTGATGGATGCGTTGGGCGCGCGCACCGCCGACGTCGTGGCGCACGACA
TCGGCAACATGGTCGCCTATGCGCTGGCGGCGCGGCATTCCGACCGGGTGACAAAACTGGTGCTGATGGACGCGCCGGTG
CCCGGCATCGGCCCATGGGAGGAGATCCTGAAGAACCCGCTGCTGTGGCACTTCCGCTTCGGCGGCCCCGACATGGAGCG
CCTGGTCGAGGGCCGCGAGCGCATCTATCTCGACCGCTTCTGGAACGAGTTCTCGGCCAAGCCTGCGAATTTCCCCGAGG
CCGCGCGCGTCCACTACGCGCAGCTCTACGCCGGCCCTGGGCGGATGCATGCCGGCTTCAGCCAGTTCGCCGCCTTCGAT
CAGGACGCGATCGACAACCGCGCCTGGCTCGCCGCCCATGGCAAGCTGACCATGCCGGTGCTCGCGATCGGCGGCAGCGC
CTCGTTCGGCCCGACGATGGCGGTGGTGGCGCGGGCCGGCGCCGACAACGTGCAGGAGAAGGTCATCGACGGCTCCGGCC
ACTGGCTGATGGAGGAGCAGCCCGCCGCGACAGTCGCCGCGATCGACGCCTTCCTCAACGCGCAGCCTGCCGCAAAGCCG
TGA

Upstream 100 bases:

>100_bases
TTTGCGTCCAGATCAAAAATCCTTGGCGTGACTACCCTCTAATACTGCGGATCGCCGCGGTCTAGCTTTGCGTGCATCGA
CAAGCCCAGGAGATCTCGCC

Downstream 100 bases:

>100_bases
TCCGCTGACCGTCAGCGGGAGCAGACAGGGGCAGCGGCGCACGGCGGAGCCCCGCATGGGCGGGGCTCGGAACTCCGATC
AATGGAACGAGGCCATGGGA

Product: putative hydrolase

Products: NA

Alternate protein names: SEH; Cytosolic epoxide hydrolase; cEH; Epoxide hydratase [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MTLPRLLQRTALAATALTALAGAALADAEEEPGHFPPAFRTQEIPTNGTTIHVRVGGTGPAVVLLHGYGDTGDMWVPLAA
KLAANHTVIVPDLRGLGLSARADKGFEKANQAEDITGVMDALGARTADVVAHDIGNMVAYALAARHSDRVTKLVLMDAPV
PGIGPWEEILKNPLLWHFRFGGPDMERLVEGRERIYLDRFWNEFSAKPANFPEAARVHYAQLYAGPGRMHAGFSQFAAFD
QDAIDNRAWLAAHGKLTMPVLAIGGSASFGPTMAVVARAGADNVQEKVIDGSGHWLMEEQPAATVAAIDAFLNAQPAAKP

Sequences:

>Translated_320_residues
MTLPRLLQRTALAATALTALAGAALADAEEEPGHFPPAFRTQEIPTNGTTIHVRVGGTGPAVVLLHGYGDTGDMWVPLAA
KLAANHTVIVPDLRGLGLSARADKGFEKANQAEDITGVMDALGARTADVVAHDIGNMVAYALAARHSDRVTKLVLMDAPV
PGIGPWEEILKNPLLWHFRFGGPDMERLVEGRERIYLDRFWNEFSAKPANFPEAARVHYAQLYAGPGRMHAGFSQFAAFD
QDAIDNRAWLAAHGKLTMPVLAIGGSASFGPTMAVVARAGADNVQEKVIDGSGHWLMEEQPAATVAAIDAFLNAQPAAKP
>Mature_319_residues
TLPRLLQRTALAATALTALAGAALADAEEEPGHFPPAFRTQEIPTNGTTIHVRVGGTGPAVVLLHGYGDTGDMWVPLAAK
LAANHTVIVPDLRGLGLSARADKGFEKANQAEDITGVMDALGARTADVVAHDIGNMVAYALAARHSDRVTKLVLMDAPVP
GIGPWEEILKNPLLWHFRFGGPDMERLVEGRERIYLDRFWNEFSAKPANFPEAARVHYAQLYAGPGRMHAGFSQFAAFDQ
DAIDNRAWLAAHGKLTMPVLAIGGSASFGPTMAVVARAGADNVQEKVIDGSGHWLMEEQPAATVAAIDAFLNAQPAAKP

Specific function: Involved in catabolic degradation of epoxides. Shows highest activity towards C6 and C7 carbocyclic epoxides. Also active towards linear 1,2-epoxyalkanes [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639
- InterPro:   IPR010497 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF06441 EHN [H]

EC number: =3.3.2.10 [H]

Molecular weight: Translated: 34077; Mature: 33946

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPRLLQRTALAATALTALAGAALADAEEEPGHFPPAFRTQEIPTNGTTIHVRVGGTGP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCC
AVVLLHGYGDTGDMWVPLAAKLAANHTVIVPDLRGLGLSARADKGFEKANQAEDITGVMD
EEEEEECCCCCCCEEHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHCHHHHHHHHHH
ALGARTADVVAHDIGNMVAYALAARHSDRVTKLVLMDAPVPGIGPWEEILKNPLLWHFRF
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHCCCEEEEEEC
GGPDMERLVEGRERIYLDRFWNEFSAKPANFPEAARVHYAQLYAGPGRMHAGFSQFAAFD
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEEHHEECCCCHHHCCHHHHHHCC
QDAIDNRAWLAAHGKLTMPVLAIGGSASFGPTMAVVARAGADNVQEKVIDGSGHWLMEEQ
HHHCCCCEEEEECCCEEEEEEEECCCCCCCCHHHHHHHCCCCHHHHHHCCCCCCEEECCC
PAATVAAIDAFLNAQPAAKP
CCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TLPRLLQRTALAATALTALAGAALADAEEEPGHFPPAFRTQEIPTNGTTIHVRVGGTGP
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCC
AVVLLHGYGDTGDMWVPLAAKLAANHTVIVPDLRGLGLSARADKGFEKANQAEDITGVMD
EEEEEECCCCCCCEEHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHCHHHHHHHHHH
ALGARTADVVAHDIGNMVAYALAARHSDRVTKLVLMDAPVPGIGPWEEILKNPLLWHFRF
HHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHCCCEEEEEEC
GGPDMERLVEGRERIYLDRFWNEFSAKPANFPEAARVHYAQLYAGPGRMHAGFSQFAAFD
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEEHHEECCCCHHHCCHHHHHHCC
QDAIDNRAWLAAHGKLTMPVLAIGGSASFGPTMAVVARAGADNVQEKVIDGSGHWLMEEQ
HHHCCCCEEEEECCCEEEEEEEECCCCCCCCHHHHHHHCCCCHHHHHHCCCCCCEEECCC
PAATVAAIDAFLNAQPAAKP
CCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9578475 [H]