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The map label for this gene is menE [H]

Identifier: 146343096

GI number: 146343096

Start: 6532750

End: 6534138

Strand: Reverse

Name: menE [H]

Synonym: BRADO6291

Alternate gene names: 146343096

Gene position: 6534138-6532750 (Counterclockwise)

Preceding gene: 146343097

Following gene: 146343095

Centisome position: 87.63

GC content: 67.67

Gene sequence:

>1389_bases
ATGTCCCCGCGTGAGGTCTTTGCGCTGCGCGACCATCTCGGCACTGACCTGACCGAGCGGACCATTTCGGATCCGCGTCA
CGTCGTCGGGCTGGCCGAGCTGTCGCGCAAAAGCTGTCTCGGCGGCCGCATCGGCGAGCTCGACGGCCGCTCGGTGCTGG
TCGCAACCTCCGGCCAGCTGCTGGCGGGCTTGGCCTTGGTCGAGATCGACGGCGTGGCCCGGCGCATGCTGCTGTGCCCG
CCGGATCTGAAGAGCGAGCATCTGACCAGCATCATCGCGGACGCCGGAATTGATGCTGTGGTCACCGACGAGCCGGAACG
CTTCGCAGATTGCGGGATCGATCGCGTGGTCCTGGTCGGGCTGCCGCAGCCCGTCGCATGCATGGCGCAGACGGTGCGGG
CGACCGAATGGCTGATGCTGACGTCGGGAACGTCGGGCGTTCCGAAGATCGTCGGCCACACGCTGGATGGCCTATGCGGC
GCCATCATCGGCGATCGACCGGCCCCTGGCGATGCGCTTCCGGTGTGGGCGACGTTCTACGACATCCGCCGCTATGGCGG
ACTGCAGATCTTTCTCCGCGCGATCGTCGGCGGCGGCTCCATGGTGCTGTCCGATCCGGGCGAGGCGCTGTCCGATCACG
TCGCGCGTCTGAATGCGAGCGGAGTCTCGCACATCTCTGGCACGCCGTCGCATTGGCGCAAGCTGCTGATGAGCGGCTCG
GCCGAGCAATTCGCTCCGCGTTATGTGCGGCTGTCGGGCGAGATCGCCGACCAAGCCGTGCTCGACGGTCTGAAGCAGGC
GTTCCCGAATTCCTCGATCGGACACGCCTATGCCTCGACGGAAGCGGGAGTCGGCTTTGCTGTCAATGACGGACTGGAGG
GCTTTCCCGCCACGCTGCTCGGCGAGGGCCTCGGTGCCGTTGACATGAAGATCGTCGACGGATCGTTGCGCATCCGCTCC
AAGCGTGCGGCGCATGCCTATGTCGGGCGCGCCGCGGCGGCGCTGACCGACGCCGACGGCTATGTCGACACCGGCGACAT
GGTTGAGCTGCGTGGCGATCGCTACTACTTCGTCGGACGGCGCAACGGCATCATCAATGTCGGCGGCCTGAAGGTGCATC
CCGAGGAGATCGAGGCCGTGATCAATGGCCATCCGGCCGTCCGGATGTCGCGCGCGAAATCGCGCCGCAGCCCGATCACC
GGCGGCATCGTCGTAGCCGAGGTCGTCCTGACGGCGGACGGCGATCCGGCGCGCGCCGACGCTGTTCGCAACGAGATCCT
CGCCGAGTGCCGCGCGCGTCTCGCGGCCCACAAGGTGCCGGCGATGCTCCGCTTCGTGCCGGCGCTCGACGTCACTCCGG
CCGGCAAGCTGGCGCGCACCGATGCGTAA

Upstream 100 bases:

>100_bases
GTCGCCCGTCTCGAGGACGATCTCGGCGTCGATCCGTTCACCCTGTCTGAAGAGGTCACGTTCCCCAACACGATCGGCGA
CTTCATCAGGGCTTATGAGA

Downstream 100 bases:

>100_bases
CGTCCTGGTCACCGGTGGAAGCCGCGGCATCGGACTTGCGATCGCCCGCAGGCTCGCCGGCGCGGGCTTTGATGTGATCG
CGGTCGCCCGACGCGAGAGC

Product: putative 2-succinylbenzoate--CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSPREVFALRDHLGTDLTERTISDPRHVVGLAELSRKSCLGGRIGELDGRSVLVATSGQLLAGLALVEIDGVARRMLLCP
PDLKSEHLTSIIADAGIDAVVTDEPERFADCGIDRVVLVGLPQPVACMAQTVRATEWLMLTSGTSGVPKIVGHTLDGLCG
AIIGDRPAPGDALPVWATFYDIRRYGGLQIFLRAIVGGGSMVLSDPGEALSDHVARLNASGVSHISGTPSHWRKLLMSGS
AEQFAPRYVRLSGEIADQAVLDGLKQAFPNSSIGHAYASTEAGVGFAVNDGLEGFPATLLGEGLGAVDMKIVDGSLRIRS
KRAAHAYVGRAAAALTDADGYVDTGDMVELRGDRYYFVGRRNGIINVGGLKVHPEEIEAVINGHPAVRMSRAKSRRSPIT
GGIVVAEVVLTADGDPARADAVRNEILAECRARLAAHKVPAMLRFVPALDVTPAGKLARTDA

Sequences:

>Translated_462_residues
MSPREVFALRDHLGTDLTERTISDPRHVVGLAELSRKSCLGGRIGELDGRSVLVATSGQLLAGLALVEIDGVARRMLLCP
PDLKSEHLTSIIADAGIDAVVTDEPERFADCGIDRVVLVGLPQPVACMAQTVRATEWLMLTSGTSGVPKIVGHTLDGLCG
AIIGDRPAPGDALPVWATFYDIRRYGGLQIFLRAIVGGGSMVLSDPGEALSDHVARLNASGVSHISGTPSHWRKLLMSGS
AEQFAPRYVRLSGEIADQAVLDGLKQAFPNSSIGHAYASTEAGVGFAVNDGLEGFPATLLGEGLGAVDMKIVDGSLRIRS
KRAAHAYVGRAAAALTDADGYVDTGDMVELRGDRYYFVGRRNGIINVGGLKVHPEEIEAVINGHPAVRMSRAKSRRSPIT
GGIVVAEVVLTADGDPARADAVRNEILAECRARLAAHKVPAMLRFVPALDVTPAGKLARTDA
>Mature_461_residues
SPREVFALRDHLGTDLTERTISDPRHVVGLAELSRKSCLGGRIGELDGRSVLVATSGQLLAGLALVEIDGVARRMLLCPP
DLKSEHLTSIIADAGIDAVVTDEPERFADCGIDRVVLVGLPQPVACMAQTVRATEWLMLTSGTSGVPKIVGHTLDGLCGA
IIGDRPAPGDALPVWATFYDIRRYGGLQIFLRAIVGGGSMVLSDPGEALSDHVARLNASGVSHISGTPSHWRKLLMSGSA
EQFAPRYVRLSGEIADQAVLDGLKQAFPNSSIGHAYASTEAGVGFAVNDGLEGFPATLLGEGLGAVDMKIVDGSLRIRSK
RAAHAYVGRAAAALTDADGYVDTGDMVELRGDRYYFVGRRNGIINVGGLKVHPEEIEAVINGHPAVRMSRAKSRRSPITG
GIVVAEVVLTADGDPARADAVRNEILAECRARLAAHKVPAMLRFVPALDVTPAGKLARTDA

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=361, Percent_Identity=25.4847645429363, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI122937307, Length=362, Percent_Identity=24.8618784530387, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI115511026, Length=203, Percent_Identity=25.615763546798, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1788595, Length=257, Percent_Identity=26.0700389105058, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 48718; Mature: 48587

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPREVFALRDHLGTDLTERTISDPRHVVGLAELSRKSCLGGRIGELDGRSVLVATSGQL
CCCHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHH
LAGLALVEIDGVARRMLLCPPDLKSEHLTSIIADAGIDAVVTDEPERFADCGIDRVVLVG
HHCCCEEEECHHHHHEEECCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHCCCCEEEEEE
LPQPVACMAQTVRATEWLMLTSGTSGVPKIVGHTLDGLCGAIIGDRPAPGDALPVWATFY
CCCCHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
DIRRYGGLQIFLRAIVGGGSMVLSDPGEALSDHVARLNASGVSHISGTPSHWRKLLMSGS
HHHHHCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCC
AEQFAPRYVRLSGEIADQAVLDGLKQAFPNSSIGHAYASTEAGVGFAVNDGLEGFPATLL
HHHHCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHH
GEGLGAVDMKIVDGSLRIRSKRAAHAYVGRAAAALTDADGYVDTGDMVELRGDRYYFVGR
HCCCCEEEEEEECCCEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEEC
RNGIINVGGLKVHPEEIEAVINGHPAVRMSRAKSRRSPITGGIVVAEVVLTADGDPARAD
CCCEEEECCEEECHHHHHHHHCCCCCEEEHHHHHHCCCCCCCEEEEEEEEECCCCCHHHH
AVRNEILAECRARLAAHKVPAMLRFVPALDVTPAGKLARTDA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SPREVFALRDHLGTDLTERTISDPRHVVGLAELSRKSCLGGRIGELDGRSVLVATSGQL
CCHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHH
LAGLALVEIDGVARRMLLCPPDLKSEHLTSIIADAGIDAVVTDEPERFADCGIDRVVLVG
HHCCCEEEECHHHHHEEECCCCCCHHHHHHHHHHCCCCEEECCCCHHHHHCCCCEEEEEE
LPQPVACMAQTVRATEWLMLTSGTSGVPKIVGHTLDGLCGAIIGDRPAPGDALPVWATFY
CCCCHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
DIRRYGGLQIFLRAIVGGGSMVLSDPGEALSDHVARLNASGVSHISGTPSHWRKLLMSGS
HHHHHCCHHHHHHHHHCCCCEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCC
AEQFAPRYVRLSGEIADQAVLDGLKQAFPNSSIGHAYASTEAGVGFAVNDGLEGFPATLL
HHHHCCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHH
GEGLGAVDMKIVDGSLRIRSKRAAHAYVGRAAAALTDADGYVDTGDMVELRGDRYYFVGR
HCCCCEEEEEEECCCEEECHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEEC
RNGIINVGGLKVHPEEIEAVINGHPAVRMSRAKSRRSPITGGIVVAEVVLTADGDPARAD
CCCEEEECCEEECHHHHHHHHCCCCCEEEHHHHHHCCCCCCCEEEEEEEEECCCCCHHHH
AVRNEILAECRARLAAHKVPAMLRFVPALDVTPAGKLARTDA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA