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The map label for this gene is azoR

Identifier: 146343005

GI number: 146343005

Start: 6418949

End: 6419593

Strand: Direct

Name: azoR

Synonym: BRADO6190

Alternate gene names: 146343005

Gene position: 6418949-6419593 (Clockwise)

Preceding gene: 146343001

Following gene: 146343006

Centisome position: 86.08

GC content: 68.68

Gene sequence:

>645_bases
ATGACCCGCCTGCTTCGCATCGACGCCAGCGCACGCCACACCGGCTCGTTCTCGCGCGATCTCGCCGATCTGTTCGTATC
CGGCTGGCTCGCTCACGAGCCGCAAGCCGAGATCGTCGTCCGTGACCTGGTCGACGCGCCGGTCCCGCACATTTCCGAGC
TCACGATCCAAGGCTACTACACGCCGCCCGCGCAGATGACGCCGCAACTCCGGACCGCGACGGCGCTGTCCGATCAGCTG
ATCGCCGAGATCCGCCGCGCCGACGTGCTGCTGCTGTCGACGCCGATGTACAATTTCTCGATCCCGTCGGCGCTGAAGGC
CTGGATCGATCACATCGTCCGCATCAACGAGACCTTCAGCTTCGACGGCGCCCGGTTCGCCGGACTGCTGCCCGGCAAGC
GCGCCTATGTCGTGACCGCTGCCGGCGCCGGCGGCTACACCGGTGACGGACCGCTCGCCCCGGCCAATTTCGTGCAGCCC
TACCTGCAATTCCTGCTCGGCTTTCTCGGCATCGCCGACGTGACGTTCGTGCCGATCGAGCAGACCACCGCGGACGCAGC
CGCAGTCGCAGACGGCCGCGCCGCAGCCGCCAGCCGGATCAGGACGCTCGTTGCCGGCGCCGTCGGCGCCCAGCAGCCCG
CCTGA

Upstream 100 bases:

>100_bases
ATTCGTGCGGAAGATGCATGATTAAAATGCAATATACTCGATTTTTCCGCCTGGGTCGTTGGCCGCAGGATGCAGCCGGT
CCAACATCGGAGGCCCATCC

Downstream 100 bases:

>100_bases
CCGAACCGCAACCACGTCCCACCGTACGAGAGATCGACCACGATCGACCAACAAGGAATCGACGATGAGCAACATCACCT
GCCTCGGCCTGGGCGCCATG

Product: putative acyl carrier protein phosphodiesterase

Products: 4'-phosphopantetheine; apoprotein

Alternate protein names: Azo-dye reductase; FMN-dependent NADH-azo compound oxidoreductase

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MTRLLRIDASARHTGSFSRDLADLFVSGWLAHEPQAEIVVRDLVDAPVPHISELTIQGYYTPPAQMTPQLRTATALSDQL
IAEIRRADVLLLSTPMYNFSIPSALKAWIDHIVRINETFSFDGARFAGLLPGKRAYVVTAAGAGGYTGDGPLAPANFVQP
YLQFLLGFLGIADVTFVPIEQTTADAAAVADGRAAAASRIRTLVAGAVGAQQPA

Sequences:

>Translated_214_residues
MTRLLRIDASARHTGSFSRDLADLFVSGWLAHEPQAEIVVRDLVDAPVPHISELTIQGYYTPPAQMTPQLRTATALSDQL
IAEIRRADVLLLSTPMYNFSIPSALKAWIDHIVRINETFSFDGARFAGLLPGKRAYVVTAAGAGGYTGDGPLAPANFVQP
YLQFLLGFLGIADVTFVPIEQTTADAAAVADGRAAAASRIRTLVAGAVGAQQPA
>Mature_213_residues
TRLLRIDASARHTGSFSRDLADLFVSGWLAHEPQAEIVVRDLVDAPVPHISELTIQGYYTPPAQMTPQLRTATALSDQLI
AEIRRADVLLLSTPMYNFSIPSALKAWIDHIVRINETFSFDGARFAGLLPGKRAYVVTAAGAGGYTGDGPLAPANFVQPY
LQFLLGFLGIADVTFVPIEQTTADAAAVADGRAAAASRIRTLVAGAVGAQQPA

Specific function: Catalyzes the reductive cleavage of azo bond in aromatic azo compounds to the corresponding amines. Requires NADH, but not NADPH, as an electron donor for its activity

COG id: COG1182

COG function: function code I; Acyl carrier protein phosphodiesterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the azoreductase type 1 family

Homologues:

Organism=Escherichia coli, GI1787680, Length=205, Percent_Identity=36.0975609756098, Blast_Score=121, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AZOR_BRASO (A4Z112)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001208053.1
- ProteinModelPortal:   A4Z112
- SMR:   A4Z112
- STRING:   A4Z112
- GeneID:   5117278
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO6190
- eggNOG:   COG1182
- HOGENOM:   HBG565834
- OMA:   TPMYNFS
- ProtClustDB:   CLSK970894
- BioCyc:   BSP376:BRADO6190-MONOMER
- HAMAP:   MF_01216
- InterPro:   IPR003680
- InterPro:   IPR023048

Pfam domain/function: PF02525 Flavodoxin_2

EC number: 3.1.4.14

Molecular weight: Translated: 22803; Mature: 22672

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLLRIDASARHTGSFSRDLADLFVSGWLAHEPQAEIVVRDLVDAPVPHISELTIQGYY
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEEEEEE
TPPAQMTPQLRTATALSDQLIAEIRRADVLLLSTPMYNFSIPSALKAWIDHIVRINETFS
CCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCC
FDGARFAGLLPGKRAYVVTAAGAGGYTGDGPLAPANFVQPYLQFLLGFLGIADVTFVPIE
CCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEECC
QTTADAAAVADGRAAAASRIRTLVAGAVGAQQPA
CCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TRLLRIDASARHTGSFSRDLADLFVSGWLAHEPQAEIVVRDLVDAPVPHISELTIQGYY
CEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCEEEEEEEE
TPPAQMTPQLRTATALSDQLIAEIRRADVLLLSTPMYNFSIPSALKAWIDHIVRINETFS
CCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCC
FDGARFAGLLPGKRAYVVTAAGAGGYTGDGPLAPANFVQPYLQFLLGFLGIADVTFVPIE
CCCCCEEECCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEECC
QTTADAAAVADGRAAAASRIRTLVAGAVGAQQPA
CCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [acyl-carrier protein]; H2O

Specific reaction: [acyl-carrier protein] + H2O = 4'-phosphopantetheine + apoprotein

General reaction: Phosphoric ester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA