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The map label for this gene is nemA [H]

Identifier: 146342968

GI number: 146342968

Start: 6376874

End: 6377998

Strand: Reverse

Name: nemA [H]

Synonym: BRADO6151

Alternate gene names: 146342968

Gene position: 6377998-6376874 (Counterclockwise)

Preceding gene: 146342971

Following gene: 146342967

Centisome position: 85.54

GC content: 68.98

Gene sequence:

>1125_bases
ATGCTGCCTGCGCTGTTTTCGCCGCTCACGATCGGTCCTTACCATCTCAAGCATCGCGTCGTGATGGCGCCGCTGACCCG
CATGCGCGCCGCCAGGGAGACGCTCGCGCCGCGCAAGCTCAACGTGGAATACTATGCGCAGCGCGCGACGCCGGGCGGCC
TGATCATCGCCGAGGCCTCGCCGGTGCTGCCGACCGGCACCGGCAGTCCGAACGTGCCGGGCATCTATAGCGAGGGGCAG
GTCGCCGGCTGGAGACAGGTGGTCGACGCCGTCCACGCCAAGGGCGGACTCATCTTCCTGCAGCTCTGGCATGTCGGCCG
TGTGTCGCATTCCTCGTTCCAGCCGGGCGGCGCGCCACCGGTGGCGCCCTCGGCGATCGCGATTCCCGACGATCTCAAGG
CGATGACGGCGGACGGCAAGGGCGCAGCCTATGAGACGCCGCGCGCGCTCGAGACCGATGAGATTCCGGCGCTCGTCGCC
GCCTATGGTCAGGCCGCGCGCAATGCGATGGCGGCCGGCTTCGACGGCGTCGAGGTGCACGGCGCGAACGGCTATCTGAT
CGAGCAGTTCCTGCAGACCCGCAGCAACCAGCGCACCGACCGCTATGGCGGCTCCATCGAGAACCGATGCCGCTTCCTGA
TCGAGGTGACGGAAGCCGTGATCGCCGCCTGCGGCGCCGACAAGGTCGGCGTGCGGCTGTCGCCTTACGGCATCGCCAAC
GGCTCCGGCGAGGCCGATCCGATGCCGCTGTATACGCACGCGATTGAGGCCCTCAACAAGCTCGGCATCGCTTACCTGCA
CCTGATCGAGCCGCGCTCCTCCGGTGCCGGCCGCGCCGATGTCAACTGGCAGAACGTGCCGTCGGCGATCGCCCTGTTCC
GGCTGGTCTGGAAGGGCGTGCTGATCGGCGCCGGCGGCTTCACCGGCGAGCCGGCGGAGGCCGCGATCCAGGCGGGGCAT
GCGGACGCCATCGCCTTCGGCCGCTACTTCATCAGCAATCCGGACCTGCCCGCCCGCCTGCAGCACGGCTGGCCGCTGAC
GCCCTACAACCGCCCGACCTTCTACGGCGGCGAGGAGAAGGGCTACACGGACTATCCGGTGTATGACGCGACGGTGACGG
CATAG

Upstream 100 bases:

>100_bases
GGAGGGTCTGCTCGGACTGAGGTGGCGCGGACATTGCCACCATCATGTTTTGCGAACAAGATGATCCCAATCGACAAACA
CAACGTCCTCAGGGGGAGAA

Downstream 100 bases:

>100_bases
TCCGCAGCTCTCTCCGTCGTCATTGCGAGGAGCGCAGCGACCAAGCAATCCAGAGTCGCGCGCGCCGCCCCTGGATTGCT
TCGCTTCGCTCGCAATGACG

Product: putative NADH-dependent flavin oxidoreductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MLPALFSPLTIGPYHLKHRVVMAPLTRMRAARETLAPRKLNVEYYAQRATPGGLIIAEASPVLPTGTGSPNVPGIYSEGQ
VAGWRQVVDAVHAKGGLIFLQLWHVGRVSHSSFQPGGAPPVAPSAIAIPDDLKAMTADGKGAAYETPRALETDEIPALVA
AYGQAARNAMAAGFDGVEVHGANGYLIEQFLQTRSNQRTDRYGGSIENRCRFLIEVTEAVIAACGADKVGVRLSPYGIAN
GSGEADPMPLYTHAIEALNKLGIAYLHLIEPRSSGAGRADVNWQNVPSAIALFRLVWKGVLIGAGGFTGEPAEAAIQAGH
ADAIAFGRYFISNPDLPARLQHGWPLTPYNRPTFYGGEEKGYTDYPVYDATVTA

Sequences:

>Translated_374_residues
MLPALFSPLTIGPYHLKHRVVMAPLTRMRAARETLAPRKLNVEYYAQRATPGGLIIAEASPVLPTGTGSPNVPGIYSEGQ
VAGWRQVVDAVHAKGGLIFLQLWHVGRVSHSSFQPGGAPPVAPSAIAIPDDLKAMTADGKGAAYETPRALETDEIPALVA
AYGQAARNAMAAGFDGVEVHGANGYLIEQFLQTRSNQRTDRYGGSIENRCRFLIEVTEAVIAACGADKVGVRLSPYGIAN
GSGEADPMPLYTHAIEALNKLGIAYLHLIEPRSSGAGRADVNWQNVPSAIALFRLVWKGVLIGAGGFTGEPAEAAIQAGH
ADAIAFGRYFISNPDLPARLQHGWPLTPYNRPTFYGGEEKGYTDYPVYDATVTA
>Mature_374_residues
MLPALFSPLTIGPYHLKHRVVMAPLTRMRAARETLAPRKLNVEYYAQRATPGGLIIAEASPVLPTGTGSPNVPGIYSEGQ
VAGWRQVVDAVHAKGGLIFLQLWHVGRVSHSSFQPGGAPPVAPSAIAIPDDLKAMTADGKGAAYETPRALETDEIPALVA
AYGQAARNAMAAGFDGVEVHGANGYLIEQFLQTRSNQRTDRYGGSIENRCRFLIEVTEAVIAACGADKVGVRLSPYGIAN
GSGEADPMPLYTHAIEALNKLGIAYLHLIEPRSSGAGRADVNWQNVPSAIALFRLVWKGVLIGAGGFTGEPAEAAIQAGH
ADAIAFGRYFISNPDLPARLQHGWPLTPYNRPTFYGGEEKGYTDYPVYDATVTA

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=371, Percent_Identity=46.0916442048518, Blast_Score=298, Evalue=3e-82,
Organism=Escherichia coli, GI1789463, Length=268, Percent_Identity=35.0746268656716, Blast_Score=121, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17559804, Length=312, Percent_Identity=28.8461538461538, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17540738, Length=313, Percent_Identity=30.0319488817891, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=354, Percent_Identity=26.5536723163842, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17559802, Length=312, Percent_Identity=28.8461538461538, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17564188, Length=313, Percent_Identity=28.4345047923323, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17565138, Length=308, Percent_Identity=27.2727272727273, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17566914, Length=198, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=381, Percent_Identity=39.6325459317585, Blast_Score=255, Evalue=9e-69,
Organism=Saccharomyces cerevisiae, GI6321973, Length=385, Percent_Identity=40.2597402597403, Blast_Score=254, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39826; Mature: 39826

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPALFSPLTIGPYHLKHRVVMAPLTRMRAARETLAPRKLNVEYYAQRATPGGLIIAEAS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCEEEEEECCCCCEEEECCC
PVLPTGTGSPNVPGIYSEGQVAGWRQVVDAVHAKGGLIFLQLWHVGRVSHSSFQPGGAPP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCC
VAPSAIAIPDDLKAMTADGKGAAYETPRALETDEIPALVAAYGQAARNAMAAGFDGVEVH
CCCCEEECCCCCHHEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
GANGYLIEQFLQTRSNQRTDRYGGSIENRCRFLIEVTEAVIAACGADKVGVRLSPYGIAN
CCCCHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCC
GSGEADPMPLYTHAIEALNKLGIAYLHLIEPRSSGAGRADVNWQNVPSAIALFRLVWKGV
CCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LIGAGGFTGEPAEAAIQAGHADAIAFGRYFISNPDLPARLQHGWPLTPYNRPTFYGGEEK
EEECCCCCCCCHHHHHHCCCCHHEEHHHHEECCCCCCHHHCCCCCCCCCCCCCCCCCCCC
GYTDYPVYDATVTA
CCCCCCCCCEEECC
>Mature Secondary Structure
MLPALFSPLTIGPYHLKHRVVMAPLTRMRAARETLAPRKLNVEYYAQRATPGGLIIAEAS
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCEEEEEECCCCCEEEECCC
PVLPTGTGSPNVPGIYSEGQVAGWRQVVDAVHAKGGLIFLQLWHVGRVSHSSFQPGGAPP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCC
VAPSAIAIPDDLKAMTADGKGAAYETPRALETDEIPALVAAYGQAARNAMAAGFDGVEVH
CCCCEEECCCCCHHEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEE
GANGYLIEQFLQTRSNQRTDRYGGSIENRCRFLIEVTEAVIAACGADKVGVRLSPYGIAN
CCCCHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCC
GSGEADPMPLYTHAIEALNKLGIAYLHLIEPRSSGAGRADVNWQNVPSAIALFRLVWKGV
CCCCCCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LIGAGGFTGEPAEAAIQAGHADAIAFGRYFISNPDLPARLQHGWPLTPYNRPTFYGGEEK
EEECCCCCCCCHHHHHHCCCCHHEEHHHHEECCCCCCHHHCCCCCCCCCCCCCCCCCCCC
GYTDYPVYDATVTA
CCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]