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The map label for this gene is mdlC [H]

Identifier: 146342967

GI number: 146342967

Start: 6375060

End: 6376724

Strand: Reverse

Name: mdlC [H]

Synonym: BRADO6150

Alternate gene names: 146342967

Gene position: 6376724-6375060 (Counterclockwise)

Preceding gene: 146342968

Following gene: 146342966

Centisome position: 85.52

GC content: 67.87

Gene sequence:

>1665_bases
ATGAAAAACCGCACCACCGGACGCTCGGCCTTTCTCGCGCTGCTCAAGGACGAGGGCATCACGCATCTGTTCGGCAACCC
CGGGACCACCGAGCTGCCGGTGATGCATGCGCTGAAGGACCATCCCGATCTCACCTATGTGATGGCGATGCAGGAGAGCC
TGGTGGTGGCGATCGCCGACGGCTTCAGCCGCGCCTCGGGACGGCTGGTCGCCTGTAACGTCCATGTCGCGCCGGGGCTC
GGCAATGCGATGGGCTCGCTCTACAACGCGCAGTTCACCGGCACGCCGATGATCCTTACCGCCGGCCAGCAGGAGCAGAG
CCACGGCCTGATGGAGCCGCTGCTTTATGGCCCGCTGGTGCGCATGGCCGAGCCGCTGGTGAAATGGGCGGTCGAGGTGA
CGCGGCTCGAGGATCTGCCGCGCATTGTCAGACGCGCCGCCAAGGTCGCGACCACGCCGCCGACCGGACCGGTGTTCATC
TCGCTGCCCGGCGACATCCTCAACGCCGAGGCCGGCATCGAGCTCGGCCGCGCGACGCGGGTCGATGCCCGCGTGCTCCC
CTCGGCGAGCGCATTGCAAGCATTGGCGCAGCGCATTTTGAAAGCCCAGCGGCCGGTGATCATCACCGGCGACGAGATCG
TCAAGAGCGATGCGCTGCGGGCCGCCGCCGACTTCGCCGCGGCGATCGGCGCGCCCGTGCATCAGCAGTCGGCGCCCTAT
GGGGCGCATTTCCTCTCCGAGCATCCCTGCTACATCGGCTCGTTGTCGCGCACGCAGAAGCAGGTGCGCGACCTCCTGGC
GCCGCATGATCTCCTGATCGTGCTCGGCGCCGATCCGCTGCGCATGTCCGTCCACAGCGAGACCGAGCCGCTGCCCGAGG
GCCTGCCGATCGTCCAGATCGGCCTCGTGGACTGGGAGCTGGGGAAGAATTTCGGCGCCGAGATCGCGCTCAAGGCCGAC
GTCCGCGAGACCTTGCTGCAACTGACGCCGCTGCTGCGCGCGCTCGGCGGTTCGGCCCTGCAGGCCGCCGCGCAAGCGCG
CATCGCGGAGCTGTCACCGAAGAACTGGAGCGCCCGCCGCCGCACGCTGACGACGCAGATCGAAGCCGCGCGCGAGCGGA
CGCCGATCGATCCGGACTATCTGGCGCTGCAGGTCGCGCAGGCCGTGCCGGAGCATGGCATCCTGGTCGACGAGGGCCTG
ACCTCGGCGCGCTACATGACGGCGTTGTGGCCGTATCGCGACCGCTACAATTATCACGGCCTCGCCTCCGGCGGCATCGG
CTGGGGCCTGCCGGCCTCGGTCGGCGTCAGCCTCGCCAATCCCGATCGCCCCGTGGTGTGCTACTCCGGCGACGGCAGCG
CGATGTATTCGATCCAGGCGCTGTGGACCGCCGCCCACCACAAGCTTCCGCTCTCGGTCGTCATCGTCAACAATGGCGGC
TATCGCATCATCAAGCAGCGCCTGCTGTCGTTCCACGGCGACGACAACTACATCGGCATGGATTTCGTCGACCCGCCGGT
GGATTTTGCCAGCCTGGCAAAGTCGCTCGGTTGCGAGGCGCTGCGCGTCAGCGATCCGCGCGAGCTGAAGCCTGTGCTGT
CGGAGGCCTTCCGCCGCCCCGGCACCAAGCTGATCGAGGTGATGGTCAGCAACGCGGTGAATTGA

Upstream 100 bases:

>100_bases
CCAGAGTCGCGCGCGCCGCCCCTGGATTGCTTCGCTTCGCTCGCAATGACGGGGAGAGCCTGCAATTACAAACAACATCG
CAGCCCGGAGGAAACGCACC

Downstream 100 bases:

>100_bases
AGAGCGCGAACTCCTTCCCCTCATGGTGAGGAGCGCCGCTTGCGGCGCGTTCGGGCGATGCGCTTGCATCGCCCTGAGAA
CCATGAGGCCACGGTCCGGG

Product: putative thiamine pyrophosphate-requiring enzyme benzoylformate decarboxylase

Products: NA

Alternate protein names: BFD; BFDC [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MKNRTTGRSAFLALLKDEGITHLFGNPGTTELPVMHALKDHPDLTYVMAMQESLVVAIADGFSRASGRLVACNVHVAPGL
GNAMGSLYNAQFTGTPMILTAGQQEQSHGLMEPLLYGPLVRMAEPLVKWAVEVTRLEDLPRIVRRAAKVATTPPTGPVFI
SLPGDILNAEAGIELGRATRVDARVLPSASALQALAQRILKAQRPVIITGDEIVKSDALRAAADFAAAIGAPVHQQSAPY
GAHFLSEHPCYIGSLSRTQKQVRDLLAPHDLLIVLGADPLRMSVHSETEPLPEGLPIVQIGLVDWELGKNFGAEIALKAD
VRETLLQLTPLLRALGGSALQAAAQARIAELSPKNWSARRRTLTTQIEAARERTPIDPDYLALQVAQAVPEHGILVDEGL
TSARYMTALWPYRDRYNYHGLASGGIGWGLPASVGVSLANPDRPVVCYSGDGSAMYSIQALWTAAHHKLPLSVVIVNNGG
YRIIKQRLLSFHGDDNYIGMDFVDPPVDFASLAKSLGCEALRVSDPRELKPVLSEAFRRPGTKLIEVMVSNAVN

Sequences:

>Translated_554_residues
MKNRTTGRSAFLALLKDEGITHLFGNPGTTELPVMHALKDHPDLTYVMAMQESLVVAIADGFSRASGRLVACNVHVAPGL
GNAMGSLYNAQFTGTPMILTAGQQEQSHGLMEPLLYGPLVRMAEPLVKWAVEVTRLEDLPRIVRRAAKVATTPPTGPVFI
SLPGDILNAEAGIELGRATRVDARVLPSASALQALAQRILKAQRPVIITGDEIVKSDALRAAADFAAAIGAPVHQQSAPY
GAHFLSEHPCYIGSLSRTQKQVRDLLAPHDLLIVLGADPLRMSVHSETEPLPEGLPIVQIGLVDWELGKNFGAEIALKAD
VRETLLQLTPLLRALGGSALQAAAQARIAELSPKNWSARRRTLTTQIEAARERTPIDPDYLALQVAQAVPEHGILVDEGL
TSARYMTALWPYRDRYNYHGLASGGIGWGLPASVGVSLANPDRPVVCYSGDGSAMYSIQALWTAAHHKLPLSVVIVNNGG
YRIIKQRLLSFHGDDNYIGMDFVDPPVDFASLAKSLGCEALRVSDPRELKPVLSEAFRRPGTKLIEVMVSNAVN
>Mature_554_residues
MKNRTTGRSAFLALLKDEGITHLFGNPGTTELPVMHALKDHPDLTYVMAMQESLVVAIADGFSRASGRLVACNVHVAPGL
GNAMGSLYNAQFTGTPMILTAGQQEQSHGLMEPLLYGPLVRMAEPLVKWAVEVTRLEDLPRIVRRAAKVATTPPTGPVFI
SLPGDILNAEAGIELGRATRVDARVLPSASALQALAQRILKAQRPVIITGDEIVKSDALRAAADFAAAIGAPVHQQSAPY
GAHFLSEHPCYIGSLSRTQKQVRDLLAPHDLLIVLGADPLRMSVHSETEPLPEGLPIVQIGLVDWELGKNFGAEIALKAD
VRETLLQLTPLLRALGGSALQAAAQARIAELSPKNWSARRRTLTTQIEAARERTPIDPDYLALQVAQAVPEHGILVDEGL
TSARYMTALWPYRDRYNYHGLASGGIGWGLPASVGVSLANPDRPVVCYSGDGSAMYSIQALWTAAHHKLPLSVVIVNNGG
YRIIKQRLLSFHGDDNYIGMDFVDPPVDFASLAKSLGCEALRVSDPRELKPVLSEAFRRPGTKLIEVMVSNAVN

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=576, Percent_Identity=20.1388888888889, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1790104, Length=558, Percent_Identity=27.7777777777778, Blast_Score=168, Evalue=1e-42,
Organism=Escherichia coli, GI87081685, Length=553, Percent_Identity=26.4014466546112, Blast_Score=152, Evalue=6e-38,
Organism=Escherichia coli, GI1786717, Length=566, Percent_Identity=25.0883392226148, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1787096, Length=501, Percent_Identity=22.3552894211577, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788716, Length=437, Percent_Identity=24.7139588100687, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17542570, Length=604, Percent_Identity=24.6688741721854, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17531299, Length=568, Percent_Identity=23.943661971831, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17531301, Length=568, Percent_Identity=23.943661971831, Blast_Score=92, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323755, Length=587, Percent_Identity=23.679727427598, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI19922626, Length=475, Percent_Identity=24.2105263157895, Blast_Score=105, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.7 [H]

Molecular weight: Translated: 59696; Mature: 59696

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRTTGRSAFLALLKDEGITHLFGNPGTTELPVMHALKDHPDLTYVMAMQESLVVAIAD
CCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHCCCCEEEEEEHHCCEEEEEEC
GFSRASGRLVACNVHVAPGLGNAMGSLYNAQFTGTPMILTAGQQEQSHGLMEPLLYGPLV
CCCCCCCEEEEEEEEECCCCCHHHHHHHCCEECCCCEEEECCCCHHHCCHHHHHHHHHHH
RMAEPLVKWAVEVTRLEDLPRIVRRAAKVATTPPTGPVFISLPGDILNAEAGIELGRATR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHCCCCCCCCCCCCC
VDARVLPSASALQALAQRILKAQRPVIITGDEIVKSDALRAAADFAAAIGAPVHQQSAPY
CCHHHCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GAHFLSEHPCYIGSLSRTQKQVRDLLAPHDLLIVLGADPLRMSVHSETEPLPEGLPIVQI
CHHHHCCCCCEECCHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCCCCCCCEEEE
GLVDWELGKNFGAEIALKADVRETLLQLTPLLRALGGSALQAAAQARIAELSPKNWSARR
EEEEEHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHH
RTLTTQIEAARERTPIDPDYLALQVAQAVPEHGILVDEGLTSARYMTALWPYRDRYNYHG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCC
LASGGIGWGLPASVGVSLANPDRPVVCYSGDGSAMYSIQALWTAAHHKLPLSVVIVNNGG
EECCCCCCCCHHHCCEEECCCCCCEEEECCCCCEEEEHHHHHHHHHCCCCEEEEEECCCC
YRIIKQRLLSFHGDDNYIGMDFVDPPVDFASLAKSLGCEALRVSDPRELKPVLSEAFRRP
HHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCC
GTKLIEVMVSNAVN
CHHHHHHHHHHCCC
>Mature Secondary Structure
MKNRTTGRSAFLALLKDEGITHLFGNPGTTELPVMHALKDHPDLTYVMAMQESLVVAIAD
CCCCCCCHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHCCCCEEEEEEHHCCEEEEEEC
GFSRASGRLVACNVHVAPGLGNAMGSLYNAQFTGTPMILTAGQQEQSHGLMEPLLYGPLV
CCCCCCCEEEEEEEEECCCCCHHHHHHHCCEECCCCEEEECCCCHHHCCHHHHHHHHHHH
RMAEPLVKWAVEVTRLEDLPRIVRRAAKVATTPPTGPVFISLPGDILNAEAGIELGRATR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHCCCCCCCCCCCCC
VDARVLPSASALQALAQRILKAQRPVIITGDEIVKSDALRAAADFAAAIGAPVHQQSAPY
CCHHHCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GAHFLSEHPCYIGSLSRTQKQVRDLLAPHDLLIVLGADPLRMSVHSETEPLPEGLPIVQI
CHHHHCCCCCEECCHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCCCCCCCEEEE
GLVDWELGKNFGAEIALKADVRETLLQLTPLLRALGGSALQAAAQARIAELSPKNWSARR
EEEEEHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHH
RTLTTQIEAARERTPIDPDYLALQVAQAVPEHGILVDEGLTSARYMTALWPYRDRYNYHG
HHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCC
LASGGIGWGLPASVGVSLANPDRPVVCYSGDGSAMYSIQALWTAAHHKLPLSVVIVNNGG
EECCCCCCCCHHHCCEEECCCCCCEEEECCCCCEEEEHHHHHHHHHCCCCEEEEEECCCC
YRIIKQRLLSFHGDDNYIGMDFVDPPVDFASLAKSLGCEALRVSDPRELKPVLSEAFRRP
HHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCC
GTKLIEVMVSNAVN
CHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2271624; 7663351; 9665697 [H]