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The map label for this gene is livJ [C]

Identifier: 146342684

GI number: 146342684

Start: 6068155

End: 6069327

Strand: Reverse

Name: livJ [C]

Synonym: BRADO5851

Alternate gene names: 146342684

Gene position: 6069327-6068155 (Counterclockwise)

Preceding gene: 146342685

Following gene: 146342683

Centisome position: 81.4

GC content: 64.19

Gene sequence:

>1173_bases
ATGAAGAAGCTCATCGCGGCCGCGCTCGCGGCCGGCCTGCTCACATCGGCGGGCGCGGCCCGCGCGGCCGACATCACCGT
CTGCATCTGGGGCACGATCACCGGGCCGGACGCACTCGTGAACGGCATGAGCTATGGCCCGCGCGACTATTTCGAGTTCC
TCAACCAGACCAAGGGCGGCATCGCCGGCAACAAGGTGAAACTGCTGCTGCTCGACGGCCGCTACAAGCTCGACGAGGAG
CTGAAGATCTACCGCCGCTGCGTCGACCAGGAGCAGGCGGTGTACGTCAACGGCTGGTCGACCGGATCGGTCAAGGCGCT
GCGCGACCAGATCACGCAGGACAAGGTGCCGTTCATGACCCAGAGCTACGCGAGCGAGGTGCTCGACCCGCAGAAGCTGC
CCTACATCTTCATGGCCGGTCCGACCTACGAGCAGCAGATGATCATCGCGCTGCGCGATCTCGCCAAGAGCGGCGGCAAG
AATCTCGTGATCATGCACCCTGACAACGAATACGGCCGCGGACCGGTCAACGTCGTGCGGCAGTCCAAGGTGATCGAGGG
CAACGGCCTGACGCTGCAGGACACGATCGAATTTCCCTATGATGCCCAGGACTTGACCGCGCAGATGCTGCGCGTCAAGG
CCAAGAACCCCGACATGGTCTACGTCCAGGCGTCGACCCCGCAGCTCCTCGTCGTGCTGCGCGACGCCGCCAAGGTCGGG
CTTTCGGCGAAGAAGTTCGTCGGCAACATCTACAACATCTCGCCGGCGATCCCCGAGCAGCTCGGCAACAATGCCGAAGG
CTTCCGCGCCATCCAGGTCTATTCGGATTTCGGCAGCGACATTCCGGCCATGGCCGATATCAAGGCGTTCGAGGCCAAGG
GCAACGAGATCCAGAAGCGCGACGTCTACTACATGAAAGGCTGGTTCGAGGGGCTCGTCATGGCCAAAGCGATCGAGGCT
GCGATCGCCAAGAACGGCGGCAAGGTGCCGGAGGACATCTCGGCGTTCCGCCAGAGCGTGCGCAACGAGATGGAGGGCCT
GAAGGACGTCGACACCGGCGGCATCGTGCCGCCCGCGAGCTATGCCAACCACCAGGGCTCGACCCAGGCGCGCATGGCCG
AGATCAAGGGCGGCAAATATGTGCCGGTCGGCGACTGGATCGACGCCCGCTGA

Upstream 100 bases:

>100_bases
GGCTGATGTCCCCACGCTGGCTGCGGAAGGCAGGCCAGGTCGACCGCGACAAGCAGGGCCTGCAGTTACAGAAGATCAAA
TCAACACAGGGAGGACTGGA

Downstream 100 bases:

>100_bases
GCGGAGAGGATCATGCTCGCGCTGAAGCAGCTTTCCGCGAATTATGACGGCGCGATCGTGGCGCTCGACGAGGTCGACGT
TAGCTGCGGTGCGGGCCAGA

Product: putative branched-chain amino acid ABC transporter substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: None

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKKLIAAALAAGLLTSAGAARAADITVCIWGTITGPDALVNGMSYGPRDYFEFLNQTKGGIAGNKVKLLLLDGRYKLDEE
LKIYRRCVDQEQAVYVNGWSTGSVKALRDQITQDKVPFMTQSYASEVLDPQKLPYIFMAGPTYEQQMIIALRDLAKSGGK
NLVIMHPDNEYGRGPVNVVRQSKVIEGNGLTLQDTIEFPYDAQDLTAQMLRVKAKNPDMVYVQASTPQLLVVLRDAAKVG
LSAKKFVGNIYNISPAIPEQLGNNAEGFRAIQVYSDFGSDIPAMADIKAFEAKGNEIQKRDVYYMKGWFEGLVMAKAIEA
AIAKNGGKVPEDISAFRQSVRNEMEGLKDVDTGGIVPPASYANHQGSTQARMAEIKGGKYVPVGDWIDAR

Sequences:

>Translated_390_residues
MKKLIAAALAAGLLTSAGAARAADITVCIWGTITGPDALVNGMSYGPRDYFEFLNQTKGGIAGNKVKLLLLDGRYKLDEE
LKIYRRCVDQEQAVYVNGWSTGSVKALRDQITQDKVPFMTQSYASEVLDPQKLPYIFMAGPTYEQQMIIALRDLAKSGGK
NLVIMHPDNEYGRGPVNVVRQSKVIEGNGLTLQDTIEFPYDAQDLTAQMLRVKAKNPDMVYVQASTPQLLVVLRDAAKVG
LSAKKFVGNIYNISPAIPEQLGNNAEGFRAIQVYSDFGSDIPAMADIKAFEAKGNEIQKRDVYYMKGWFEGLVMAKAIEA
AIAKNGGKVPEDISAFRQSVRNEMEGLKDVDTGGIVPPASYANHQGSTQARMAEIKGGKYVPVGDWIDAR
>Mature_390_residues
MKKLIAAALAAGLLTSAGAARAADITVCIWGTITGPDALVNGMSYGPRDYFEFLNQTKGGIAGNKVKLLLLDGRYKLDEE
LKIYRRCVDQEQAVYVNGWSTGSVKALRDQITQDKVPFMTQSYASEVLDPQKLPYIFMAGPTYEQQMIIALRDLAKSGGK
NLVIMHPDNEYGRGPVNVVRQSKVIEGNGLTLQDTIEFPYDAQDLTAQMLRVKAKNPDMVYVQASTPQLLVVLRDAAKVG
LSAKKFVGNIYNISPAIPEQLGNNAEGFRAIQVYSDFGSDIPAMADIKAFEAKGNEIQKRDVYYMKGWFEGLVMAKAIEA
AIAKNGGKVPEDISAFRQSVRNEMEGLKDVDTGGIVPPASYANHQGSTQARMAEIKGGKYVPVGDWIDAR

Specific function: This Protein Is A Component Of The Leucine, Isoleucine, Valine, (Threonine) Transport System, Which Is One Of The Two Periplasmic Binding Protein-Dependent Transport Systems Of The High-Affinity Transport Of The Branched-Chain Amino Acids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 42600; Mature: 42600

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLIAAALAAGLLTSAGAARAADITVCIWGTITGPDALVNGMSYGPRDYFEFLNQTKGG
CHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHCCCC
IAGNKVKLLLLDGRYKLDEELKIYRRCVDQEQAVYVNGWSTGSVKALRDQITQDKVPFMT
CCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHH
QSYASEVLDPQKLPYIFMAGPTYEQQMIIALRDLAKSGGKNLVIMHPDNEYGRGPVNVVR
HHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
QSKVIEGNGLTLQDTIEFPYDAQDLTAQMLRVKAKNPDMVYVQASTPQLLVVLRDAAKVG
HCEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEEHHHHHC
LSAKKFVGNIYNISPAIPEQLGNNAEGFRAIQVYSDFGSDIPAMADIKAFEAKGNEIQKR
CCHHHHHCHHHCCCCCCHHHHCCCCCCEEEEEEEHHCCCCCCCHHHHHHHHCCCCCCHHC
DVYYMKGWFEGLVMAKAIEAAIAKNGGKVPEDISAFRQSVRNEMEGLKDVDTGGIVPPAS
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
YANHQGSTQARMAEIKGGKYVPVGDWIDAR
HCCCCCCCHHHHHHHCCCEEECCCCCCCCC
>Mature Secondary Structure
MKKLIAAALAAGLLTSAGAARAADITVCIWGTITGPDALVNGMSYGPRDYFEFLNQTKGG
CHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHHHCCCCCCHHHHHHHHHHCCCC
IAGNKVKLLLLDGRYKLDEELKIYRRCVDQEQAVYVNGWSTGSVKALRDQITQDKVPFMT
CCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHH
QSYASEVLDPQKLPYIFMAGPTYEQQMIIALRDLAKSGGKNLVIMHPDNEYGRGPVNVVR
HHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHH
QSKVIEGNGLTLQDTIEFPYDAQDLTAQMLRVKAKNPDMVYVQASTPQLLVVLRDAAKVG
HCEEECCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEEHHHHHC
LSAKKFVGNIYNISPAIPEQLGNNAEGFRAIQVYSDFGSDIPAMADIKAFEAKGNEIQKR
CCHHHHHCHHHCCCCCCHHHHCCCCCCEEEEEEEHHCCCCCCCHHHHHHHHCCCCCCHHC
DVYYMKGWFEGLVMAKAIEAAIAKNGGKVPEDISAFRQSVRNEMEGLKDVDTGGIVPPAS
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCH
YANHQGSTQARMAEIKGGKYVPVGDWIDAR
HCCCCCCCHHHHHHHCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA