Definition Bradyrhizobium sp. ORS278 chromosome, complete genome.
Accession NC_009445
Length 7,456,587

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The map label for this gene is livF [H]

Identifier: 146342683

GI number: 146342683

Start: 6067429

End: 6068142

Strand: Reverse

Name: livF [H]

Synonym: BRADO5850

Alternate gene names: 146342683

Gene position: 6068142-6067429 (Counterclockwise)

Preceding gene: 146342684

Following gene: 146342682

Centisome position: 81.38

GC content: 64.43

Gene sequence:

>714_bases
ATGCTCGCGCTGAAGCAGCTTTCCGCGAATTATGACGGCGCGATCGTGGCGCTCGACGAGGTCGACGTTAGCTGCGGTGC
GGGCCAGATCGTGGCGCTGCTCGGGGCCAACGGCGCCGGGAAGACCACGCTGCTGAAGGCCGTCGCCGGCATGCTGCCGT
TCGATCGCGGCGACATCGTCGCCGGCGACATCCTGTTCGAGGACGTCTCGCTGCTCCGCCGACATCCGGCCGACATCTCG
CGGCGCGGCATCGCACTCGTCCAGGATGGCCGGCAGTGCTTCCGGCAGCTGACCGTAGCGGAAAACCTGCAAGCCGCGTC
CTTTGCGCATTCGAAGGGCGCGTCCGAGCGGCTGGAGCTCGTCCAGAGCTATTTCCCGTTCCTGCGCGAGATGATGGACC
GGAAAGCGGGACTGCTGTCCGGAGGTCAGCTGCAAATGCTGGTGATCGGCATGGCGATCATGTGCAATCCCAGGCTTCTG
ATGCTAGACGAGCCAAGTCTCGGCCTGTCGCCGGTGATGGTGCAGAGCGTGTTCGACGCGATCGAGCGCATGCATCGCGA
GCTGAAGATGACGATCGTCGTCGCGGAGCAGACCGTGCCGCGGCTGCTCAAGATCGCCTCCGACGCCTATGTGCTGTCGC
GGGGCCGGATCGTGATGCATGCGCCGCCATCCGAGATGACCGAGGAAGAGCTGCAGAAGGTCTATTTGAACTAG

Upstream 100 bases:

>100_bases
CAACCACCAGGGCTCGACCCAGGCGCGCATGGCCGAGATCAAGGGCGGCAAATATGTGCCGGTCGGCGACTGGATCGACG
CCCGCTGAGCGGAGAGGATC

Downstream 100 bases:

>100_bases
CATGTGGCCCGCGCTGCCCGTCACCGGCGTGACGCCGCGGAGCGCATCCAAGGATCATAAGAAGTCGGAGAGCCGCCATG
AACGCCAATGTCAAACTGCC

Product: putative branched-chain amino acid ABC transporter ATP binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein F [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MLALKQLSANYDGAIVALDEVDVSCGAGQIVALLGANGAGKTTLLKAVAGMLPFDRGDIVAGDILFEDVSLLRRHPADIS
RRGIALVQDGRQCFRQLTVAENLQAASFAHSKGASERLELVQSYFPFLREMMDRKAGLLSGGQLQMLVIGMAIMCNPRLL
MLDEPSLGLSPVMVQSVFDAIERMHRELKMTIVVAEQTVPRLLKIASDAYVLSRGRIVMHAPPSEMTEEELQKVYLN

Sequences:

>Translated_237_residues
MLALKQLSANYDGAIVALDEVDVSCGAGQIVALLGANGAGKTTLLKAVAGMLPFDRGDIVAGDILFEDVSLLRRHPADIS
RRGIALVQDGRQCFRQLTVAENLQAASFAHSKGASERLELVQSYFPFLREMMDRKAGLLSGGQLQMLVIGMAIMCNPRLL
MLDEPSLGLSPVMVQSVFDAIERMHRELKMTIVVAEQTVPRLLKIASDAYVLSRGRIVMHAPPSEMTEEELQKVYLN
>Mature_237_residues
MLALKQLSANYDGAIVALDEVDVSCGAGQIVALLGANGAGKTTLLKAVAGMLPFDRGDIVAGDILFEDVSLLRRHPADIS
RRGIALVQDGRQCFRQLTVAENLQAASFAHSKGASERLELVQSYFPFLREMMDRKAGLLSGGQLQMLVIGMAIMCNPRLL
MLDEPSLGLSPVMVQSVFDAIERMHRELKMTIVVAEQTVPRLLKIASDAYVLSRGRIVMHAPPSEMTEEELQKVYLN

Specific function: Component of the leucine-specific transport system [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI153792144, Length=210, Percent_Identity=29.5238095238095, Blast_Score=74, Evalue=8e-14,
Organism=Homo sapiens, GI31657092, Length=242, Percent_Identity=28.5123966942149, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI27262624, Length=212, Percent_Identity=27.8301886792453, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI27262626, Length=212, Percent_Identity=27.8301886792453, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI105990541, Length=222, Percent_Identity=27.9279279279279, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI116734710, Length=172, Percent_Identity=30.2325581395349, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI27477115, Length=189, Percent_Identity=32.2751322751323, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI62526033, Length=207, Percent_Identity=28.9855072463768, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI27436953, Length=211, Percent_Identity=28.9099526066351, Blast_Score=66, Evalue=2e-11,
Organism=Homo sapiens, GI21536376, Length=173, Percent_Identity=28.3236994219653, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI87082268, Length=220, Percent_Identity=45, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1789593, Length=242, Percent_Identity=32.2314049586777, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788761, Length=236, Percent_Identity=28.3898305084746, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788897, Length=213, Percent_Identity=30.9859154929577, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790467, Length=217, Percent_Identity=27.6497695852535, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1789864, Length=231, Percent_Identity=26.8398268398268, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787370, Length=226, Percent_Identity=30.0884955752212, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1787029, Length=247, Percent_Identity=26.3157894736842, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI87082267, Length=230, Percent_Identity=26.0869565217391, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081791, Length=240, Percent_Identity=30.8333333333333, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789991, Length=236, Percent_Identity=25.8474576271186, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI48994943, Length=200, Percent_Identity=33, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788225, Length=230, Percent_Identity=29.1304347826087, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1787792, Length=233, Percent_Identity=27.8969957081545, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1789672, Length=231, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=7e-16,
Organism=Escherichia coli, GI48995001, Length=245, Percent_Identity=30.2040816326531, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788210, Length=224, Percent_Identity=26.7857142857143, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787089, Length=246, Percent_Identity=29.2682926829268, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788450, Length=220, Percent_Identity=27.7272727272727, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789586, Length=214, Percent_Identity=27.5700934579439, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786563, Length=228, Percent_Identity=28.5087719298246, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87081709, Length=224, Percent_Identity=26.3392857142857, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788472, Length=237, Percent_Identity=25.7383966244726, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786872, Length=228, Percent_Identity=27.6315789473684, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790190, Length=227, Percent_Identity=23.7885462555066, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786253, Length=206, Percent_Identity=29.6116504854369, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1786398, Length=221, Percent_Identity=26.6968325791855, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI87081782, Length=224, Percent_Identity=25.4464285714286, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1786319, Length=194, Percent_Identity=29.3814432989691, Blast_Score=67, Evalue=7e-13,
Organism=Escherichia coli, GI1790544, Length=231, Percent_Identity=24.6753246753247, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790525, Length=228, Percent_Identity=26.7543859649123, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1786803, Length=238, Percent_Identity=23.5294117647059, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787712, Length=216, Percent_Identity=25, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1787164, Length=216, Percent_Identity=27.3148148148148, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI71983510, Length=207, Percent_Identity=28.9855072463768, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71983516, Length=207, Percent_Identity=28.9855072463768, Blast_Score=68, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71983523, Length=207, Percent_Identity=28.9855072463768, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI115533608, Length=186, Percent_Identity=29.5698924731183, Blast_Score=67, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17510237, Length=210, Percent_Identity=24.7619047619048, Blast_Score=64, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6324498, Length=233, Percent_Identity=26.1802575107296, Blast_Score=72, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6322177, Length=187, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24581383, Length=238, Percent_Identity=27.7310924369748, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24581385, Length=238, Percent_Identity=27.7310924369748, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24581387, Length=238, Percent_Identity=27.7310924369748, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI28574533, Length=206, Percent_Identity=29.6116504854369, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24650855, Length=187, Percent_Identity=24.5989304812834, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24650853, Length=187, Percent_Identity=24.5989304812834, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24582014, Length=190, Percent_Identity=27.3684210526316, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24582012, Length=190, Percent_Identity=27.3684210526316, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281362751, Length=187, Percent_Identity=24.5989304812834, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24662800, Length=208, Percent_Identity=27.8846153846154, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 25867; Mature: 25867

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MLALKQLSANYDGAIVALDEVDVSCGAGQIVALLGANGAGKTTLLKAVAGMLPFDRGDIV
CCCHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEE
AGDILFEDVSLLRRHPADISRRGIALVQDGRQCFRQLTVAENLQAASFAHSKGASERLEL
EHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VQSYFPFLREMMDRKAGLLSGGQLQMLVIGMAIMCNPRLLMLDEPSLGLSPVMVQSVFDA
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHH
IERMHRELKMTIVVAEQTVPRLLKIASDAYVLSRGRIVMHAPPSEMTEEELQKVYLN
HHHHHHHHHEEEEEEHHHHHHHHHHHCCCEEEECCEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLALKQLSANYDGAIVALDEVDVSCGAGQIVALLGANGAGKTTLLKAVAGMLPFDRGDIV
CCCHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCEE
AGDILFEDVSLLRRHPADISRRGIALVQDGRQCFRQLTVAENLQAASFAHSKGASERLEL
EHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VQSYFPFLREMMDRKAGLLSGGQLQMLVIGMAIMCNPRLLMLDEPSLGLSPVMVQSVFDA
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHH
IERMHRELKMTIVVAEQTVPRLLKIASDAYVLSRGRIVMHAPPSEMTEEELQKVYLN
HHHHHHHHHEEEEEEHHHHHHHHHHHCCCEEEECCEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]