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The map label for this gene is mycA [H]

Identifier: 146342532

GI number: 146342532

Start: 5896209

End: 5897873

Strand: Direct

Name: mycA [H]

Synonym: BRADO5694

Alternate gene names: 146342532

Gene position: 5896209-5897873 (Clockwise)

Preceding gene: 146342524

Following gene: 146342536

Centisome position: 79.07

GC content: 65.17

Gene sequence:

>1665_bases
ATGATGGAAACCTCGATTCCTTATGTATCGCTCGCCGTTGGCGCTGCCGTGACGGCCGCCGTCGCCCCGAAGGTCAAGGC
CCGCATCGAATTGTCGCGCGCCAAGCACCGTTCGCTGGCCGGCCATTCCAAGATGTCCCGCCGGATCTCCAAGCTGATCC
CGCGCTATGAATTCGACATCGACGATTTCTTCCGCTCCGACGGCGCGCCGCCTGACGTCGCGACCAGGCGTCAGGACGGC
TTCTTCCGGCTCGGCGCGCAGTTCCAGGAGAAATTCGCCAAGAGCCGCGCGTTGACGGCGGAGGCCGCAAGCCGCATCTC
CGATTTGCAGTTCACCCAGGCTTACCGGGTGCCGTTCCAGTACAGCAGGCTGGTCAAGGAACAGCTCGGCGCCGGATCGT
TCATGCAGTCGTCGTCCGGCGTCACCATCACCGATCTCGACGGCAACCAGTTCTACGACCTCACCGGCTCCTACGGCGTC
AACATCTTCGGCTACGATTTCTACAAGGAGTGCATCGCCGCCGCCGACCGCCGCGCGCAGGCGCTCGGCCCGGTGCTCGG
GCTGTATCATCCGGTGGTTGCCGACAATGTGAAACGGCTGACCGAACTGTCCGGCATGGACGAGGTGTCGTTCCACATGT
CCGGCACCGAGGCGGTGATGCAGGCGGTGCGGCTCGCCCGCTACCATACCGGCCGCAGCCATCTGGTGCGCTTCGCCGGC
GCCTATCACGGCTGGTGGGGCGACGTGCAGCCCGGCGTCGGCAATCCGATCGCGCCGCACGAGACCTATACGCTCGCAGA
AATGTCGGAGCGGACCCTGCATGTGCTGCGCACGCGGCGCGACATCGCCTGCGTGCTGGTCAACCCGCTGCAGGCGCTGC
ATCCGAACAGCAATGCGCCCGGCGATTCGGCGCTGGTCGATTCGTCGCGCAAGGGCCGTTACGACCGCGAGGCCTACACG
GCGTGGCTGAAGCAGCTGCGCGAGGTGTGTACCGAACGCGGCATCGTGCTGCTGTTCGACGAGGTCTTCCTCGGCTTCCG
TCTCGCGCCGGGCGGCGCCCAGGAGTATTTCGGCGTCAAGGCCGACATGGTGACCTACGGCAAGAGCCTCGGCGGCGGTC
TCCCGATCGGCGTGGTCTGCGGCAAGAAGGAATACATGCGCCGCTTCCGTGACGACCGTCCCGCCGACATCTGCTTCGCC
CGCGGCACCTTCAACTCGCACCCCTACGTGATGACGGCGATGGACGAGTTCCTGAGCCGGCTCCACAGTCCGAACTTCCG
CGCCGTCTACAACGGCCTCGACGACACCTGGAACGGCCGCGCCTCCAAGCTCAATGCGGAGCTCACCGCCAGGGACCTGC
CGGTGCGCGTGTCCAACATGCAGTCGATCTGGCTGGTCGAGTACACCGTGCCGTCGCGCTACAATTGGATGCTGCAATAC
TATCTGCGCGCCGAAGGCCTGGCGCTCAGCTGGGTCGGCACCGGCCGCTTCATCTTCAGCCTGAACTACACCGACGCCGA
TTTCGCCGAGGTCACCAAACGCTTCGTCGCGGCGGCCGAGAAGATGAAGCAGGACGGCTGGTGGTGGAACGACGGCACCC
TCACCAACAAGGCGATCAAGCGGCTGATCCTGAAAGAGATGCTCGGGGCGGTCATCAAGCGCTGA

Upstream 100 bases:

>100_bases
TTGGTGAGCAGGAAGTTAAGATCTTCCTGTTGGGTGAAGGAAGCGATCAGGCTTTTGACTGTCATGAATCTGTCAGCAGG
TGGGGTTAGCGCTTTTGGAC

Downstream 100 bases:

>100_bases
GTTTTGCGCCTCCCCCGCTTGCGGGGGAGGTCGGCGGGCTCGCAGAGCCCGACGGGTGGGAGCCGCCGCACGACGCGCTC
TCACGGTGAGCGGAGCAAGC

Product: putative glutamate-1-semialdehyde 2, 1-aminomutase

Products: 5-Amino-4-oxopentanoate [C]

Alternate protein names: Glutamate-1-semialdehyde aminotransferase; GSA-AT; ATP-dependent asparagine adenylase 1; AsnA 1; Asparagine activase 1 [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MMETSIPYVSLAVGAAVTAAVAPKVKARIELSRAKHRSLAGHSKMSRRISKLIPRYEFDIDDFFRSDGAPPDVATRRQDG
FFRLGAQFQEKFAKSRALTAEAASRISDLQFTQAYRVPFQYSRLVKEQLGAGSFMQSSSGVTITDLDGNQFYDLTGSYGV
NIFGYDFYKECIAAADRRAQALGPVLGLYHPVVADNVKRLTELSGMDEVSFHMSGTEAVMQAVRLARYHTGRSHLVRFAG
AYHGWWGDVQPGVGNPIAPHETYTLAEMSERTLHVLRTRRDIACVLVNPLQALHPNSNAPGDSALVDSSRKGRYDREAYT
AWLKQLREVCTERGIVLLFDEVFLGFRLAPGGAQEYFGVKADMVTYGKSLGGGLPIGVVCGKKEYMRRFRDDRPADICFA
RGTFNSHPYVMTAMDEFLSRLHSPNFRAVYNGLDDTWNGRASKLNAELTARDLPVRVSNMQSIWLVEYTVPSRYNWMLQY
YLRAEGLALSWVGTGRFIFSLNYTDADFAEVTKRFVAAAEKMKQDGWWWNDGTLTNKAIKRLILKEMLGAVIKR

Sequences:

>Translated_554_residues
MMETSIPYVSLAVGAAVTAAVAPKVKARIELSRAKHRSLAGHSKMSRRISKLIPRYEFDIDDFFRSDGAPPDVATRRQDG
FFRLGAQFQEKFAKSRALTAEAASRISDLQFTQAYRVPFQYSRLVKEQLGAGSFMQSSSGVTITDLDGNQFYDLTGSYGV
NIFGYDFYKECIAAADRRAQALGPVLGLYHPVVADNVKRLTELSGMDEVSFHMSGTEAVMQAVRLARYHTGRSHLVRFAG
AYHGWWGDVQPGVGNPIAPHETYTLAEMSERTLHVLRTRRDIACVLVNPLQALHPNSNAPGDSALVDSSRKGRYDREAYT
AWLKQLREVCTERGIVLLFDEVFLGFRLAPGGAQEYFGVKADMVTYGKSLGGGLPIGVVCGKKEYMRRFRDDRPADICFA
RGTFNSHPYVMTAMDEFLSRLHSPNFRAVYNGLDDTWNGRASKLNAELTARDLPVRVSNMQSIWLVEYTVPSRYNWMLQY
YLRAEGLALSWVGTGRFIFSLNYTDADFAEVTKRFVAAAEKMKQDGWWWNDGTLTNKAIKRLILKEMLGAVIKR
>Mature_554_residues
MMETSIPYVSLAVGAAVTAAVAPKVKARIELSRAKHRSLAGHSKMSRRISKLIPRYEFDIDDFFRSDGAPPDVATRRQDG
FFRLGAQFQEKFAKSRALTAEAASRISDLQFTQAYRVPFQYSRLVKEQLGAGSFMQSSSGVTITDLDGNQFYDLTGSYGV
NIFGYDFYKECIAAADRRAQALGPVLGLYHPVVADNVKRLTELSGMDEVSFHMSGTEAVMQAVRLARYHTGRSHLVRFAG
AYHGWWGDVQPGVGNPIAPHETYTLAEMSERTLHVLRTRRDIACVLVNPLQALHPNSNAPGDSALVDSSRKGRYDREAYT
AWLKQLREVCTERGIVLLFDEVFLGFRLAPGGAQEYFGVKADMVTYGKSLGGGLPIGVVCGKKEYMRRFRDDRPADICFA
RGTFNSHPYVMTAMDEFLSRLHSPNFRAVYNGLDDTWNGRASKLNAELTARDLPVRVSNMQSIWLVEYTVPSRYNWMLQY
YLRAEGLALSWVGTGRFIFSLNYTDADFAEVTKRFVAAAEKMKQDGWWWNDGTLTNKAIKRLILKEMLGAVIKR

Specific function: This protein is a multifunctional enzyme, able to activate a long chain fatty acid and link it with the amino acid Asn as part of the synthesis of mycosubtilin. The activation sites consist of individual domains [H]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 acyl carrier domains [H]

Homologues:

Organism=Escherichia coli, GI1786349, Length=376, Percent_Identity=28.1914893617021, Blast_Score=128, Evalue=1e-30,
Organism=Escherichia coli, GI1787560, Length=439, Percent_Identity=24.8291571753986, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1789016, Length=318, Percent_Identity=26.7295597484277, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788044, Length=267, Percent_Identity=27.3408239700375, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR005814
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR023213
- InterPro:   IPR001242
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR006162
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00202 Aminotran_3; PF00501 AMP-binding; PF00668 Condensation; PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C; PF00550 PP-binding [H]

EC number: 5.4.3.8 [C]

Molecular weight: Translated: 62208; Mature: 62208

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMETSIPYVSLAVGAAVTAAVAPKVKARIELSRAKHRSLAGHSKMSRRISKLIPRYEFDI
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
DDFFRSDGAPPDVATRRQDGFFRLGAQFQEKFAKSRALTAEAASRISDLQFTQAYRVPFQ
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
YSRLVKEQLGAGSFMQSSSGVTITDLDGNQFYDLTGSYGVNIFGYDFYKECIAAADRRAQ
HHHHHHHHHCCCCCEECCCCCEEEECCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHH
ALGPVLGLYHPVVADNVKRLTELSGMDEVSFHMSGTEAVMQAVRLARYHTGRSHLVRFAG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHH
AYHGWWGDVQPGVGNPIAPHETYTLAEMSERTLHVLRTRRDIACVLVNPLQALHPNSNAP
HHCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCCCC
GDSALVDSSRKGRYDREAYTAWLKQLREVCTERGIVLLFDEVFLGFRLAPGGAQEYFGVK
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHCEEECCCCCHHHCCCC
ADMVTYGKSLGGGLPIGVVCGKKEYMRRFRDDRPADICFARGTFNSHPYVMTAMDEFLSR
HHHHHHHHHHCCCCCEEEEECCHHHHHHHCCCCCCCEEEECCCCCCCCEEHHHHHHHHHH
LHSPNFRAVYNGLDDTWNGRASKLNAELTARDLPVRVSNMQSIWLVEYTVPSRYNWMLQY
HCCCCCHHHHCCCCCCCCCCHHHCCCEEECCCCCEEECCCCEEEEEEEECCCCHHHHHHH
YLRAEGLALSWVGTGRFIFSLNYTDADFAEVTKRFVAAAEKMKQDGWWWNDGTLTNKAIK
HHHHCCEEEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHH
RLILKEMLGAVIKR
HHHHHHHHHHHHCC
>Mature Secondary Structure
MMETSIPYVSLAVGAAVTAAVAPKVKARIELSRAKHRSLAGHSKMSRRISKLIPRYEFDI
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
DDFFRSDGAPPDVATRRQDGFFRLGAQFQEKFAKSRALTAEAASRISDLQFTQAYRVPFQ
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
YSRLVKEQLGAGSFMQSSSGVTITDLDGNQFYDLTGSYGVNIFGYDFYKECIAAADRRAQ
HHHHHHHHHCCCCCEECCCCCEEEECCCCEEEEECCCCCCEEEEHHHHHHHHHHHHHHHH
ALGPVLGLYHPVVADNVKRLTELSGMDEVSFHMSGTEAVMQAVRLARYHTGRSHLVRFAG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHH
AYHGWWGDVQPGVGNPIAPHETYTLAEMSERTLHVLRTRRDIACVLVNPLQALHPNSNAP
HHCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCCCC
GDSALVDSSRKGRYDREAYTAWLKQLREVCTERGIVLLFDEVFLGFRLAPGGAQEYFGVK
CCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHCEEECCCCCHHHCCCC
ADMVTYGKSLGGGLPIGVVCGKKEYMRRFRDDRPADICFARGTFNSHPYVMTAMDEFLSR
HHHHHHHHHHCCCCCEEEEECCHHHHHHHCCCCCCCEEEECCCCCCCCEEHHHHHHHHHH
LHSPNFRAVYNGLDDTWNGRASKLNAELTARDLPVRVSNMQSIWLVEYTVPSRYNWMLQY
HCCCCCHHHHCCCCCCCCCCHHHCCCEEECCCCCEEECCCCEEEEEEEECCCCHHHHHHH
YLRAEGLALSWVGTGRFIFSLNYTDADFAEVTKRFVAAAEKMKQDGWWWNDGTLTNKAIK
HHHHCCEEEEEEECCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHH
RLILKEMLGAVIKR
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Ethanolamine; Methylamine [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-Glutamate 1-semialdehyde [C]

Specific reaction: L-Glutamate 1-semialdehyde --> 5-Amino-4-oxopentanoate [C]

General reaction: Group transfer (intramolecular amino group isomerization) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10557314 [H]