Click here to switch to the map view.

The map label for this gene is nylA [H]

Identifier: 146342452

GI number: 146342452

Start: 5799077

End: 5800576

Strand: Reverse

Name: nylA [H]

Synonym: BRADO5611

Alternate gene names: 146342452

Gene position: 5800576-5799077 (Counterclockwise)

Preceding gene: 146342458

Following gene: 146342450

Centisome position: 77.79

GC content: 70.67

Gene sequence:

>1500_bases
GTGAAGCTCCAGGACTTTCTGCAGCGCGACGCGACCGCGCTGGCCGAAGCCGTACGCGCCGGCGAGACGACCGCTGCCGA
GCTGCTCCAGCTTGCGATGCAGCAGAGCGAGCGCGTCCAGCCGCGCACCAACGCCATCTGCACGATGATGGAGCGCGAGG
CGCGCGCGCAGCTGGCCAGGCCAGCGACCGGGCCTTTCACCGGCGTGCCGTTTCTGATCAAGGACTGCGCGCAGGACTAT
GCTGGCCTGCCGACGGCCTATGGCAGCAAGGCGCTGTCCGGCCGCGTGGCAACCGAGCATGCCTCCGTGGTCCGGCGCTA
CCTCGCGGCCGGTCTCGTGATCTTCGGCAAGACCAACCTCCCGGAGCTCGCGCTCAAGGGCGTCTCCGACTCGCGCGCTT
TCGGGCGCGTCAACAATCCCTGGAATGCGGCGCATACGCCCGGCGGCTCCAGCGGCGGTGCGGCGGCAGCGGTCGCCTCG
GGCGTCGTGCCGATGGCCGCTGGCAATGACGGCGGCGGATCGATCCGCATTCCCGCCGCCTGCTGCGGTCTGTTCGGGCT
GAAGCCGTCGCGCGGGCTGGTGTCGTCCGGGCCGGGCTTCGGCGAATACTGGTTCGGCGCCTCCTGCGAAGGCGTGCTCT
CGCGCAGCGTCCGCGACACCGCGGCAGCGCTCGACGTGATCTCGGGCGCCGAGCCCGGCGATCCCTTTCTCGTCGCGGAG
CCCGGCGTGGTGTTCGCGAATGCCGTCACACGCGATCCGCCGCGCCTGCGCATCGGGGTCACGACGGCATCGCCGATCGG
CACCGAGGTGCACGCCGAGGCGAAGCTCGCGGTCGAGACCACCGTGAAGCTGTTGCGTGGACTCGGCCATGAGGTCGAGG
AGGCCGCGCCCGAGATCGACGGCGCCGCGCTCGCGACCTCGTTCCTGCACATCTATTTCGGCCAGGTCGCCGCCTTCGTG
GCCGAGGCTCGCGCCAACGGTGCGCGTGGCGACCAGTTCGAGCTGCTGACCCGCGTGCTGGCGACGCTCGGCAGCGCGAT
CTCGGCCGGCGCGCTGACGACGCAACTGCTCAAATGGAATGAGTTTGCCCGCGCGCTCGCGCGCTTCCATGCCCGCTACG
ACCTGCTGCTGACCCCGACGCTCGCGCATCCGCCGATCCGCCACGGCCAGGGCGATCCCTCGACCGCCGAGCAGACCCTG
CTCGACATGCTCGACCGCATCGGGCTGCTCGGGCTGTTGACGCGCTGGGGCGCGCTGGACGGCATGATCGACAAGATCGC
CCGCGACAGCCTGCAATATGTGCCGTTCACCCAGCTTGCCAATCTCACGGGAACGCCGGCGATGAGCGTGCCGCTGCACT
GGACCGCAGACGGCCTGCCGCTCGGCGTGCAGTTCACCGGCCGGCTCGGCGACGAGGCGCGGCTTCTGCAGCTCGCCCGC
CAGCTCGAGCAGGCGCAGCCCTGGTTCGACCGGTTGCCGGACTGGGTGCGATCGGACTAA

Upstream 100 bases:

>100_bases
GTGCTGCCCTGATTTTGCCGCGTTCGATCCGGCGGTGCCGCCGTTGCAGCCAGAATCAGTTACCATCACGGCGGCCGTTT
GCGGTCACGGGGAGGAGAAT

Downstream 100 bases:

>100_bases
TTCATCGCTTCGCCGGCATGCTGATGGCGCGGCCGAGCCCGTCCGGTCGCGGCAGCGCGGCATGCGCCTGCAGCACGCGG
GAAATGTTGGCCTGGATGTC

Product: putative amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MKLQDFLQRDATALAEAVRAGETTAAELLQLAMQQSERVQPRTNAICTMMEREARAQLARPATGPFTGVPFLIKDCAQDY
AGLPTAYGSKALSGRVATEHASVVRRYLAAGLVIFGKTNLPELALKGVSDSRAFGRVNNPWNAAHTPGGSSGGAAAAVAS
GVVPMAAGNDGGGSIRIPAACCGLFGLKPSRGLVSSGPGFGEYWFGASCEGVLSRSVRDTAAALDVISGAEPGDPFLVAE
PGVVFANAVTRDPPRLRIGVTTASPIGTEVHAEAKLAVETTVKLLRGLGHEVEEAAPEIDGAALATSFLHIYFGQVAAFV
AEARANGARGDQFELLTRVLATLGSAISAGALTTQLLKWNEFARALARFHARYDLLLTPTLAHPPIRHGQGDPSTAEQTL
LDMLDRIGLLGLLTRWGALDGMIDKIARDSLQYVPFTQLANLTGTPAMSVPLHWTADGLPLGVQFTGRLGDEARLLQLAR
QLEQAQPWFDRLPDWVRSD

Sequences:

>Translated_499_residues
MKLQDFLQRDATALAEAVRAGETTAAELLQLAMQQSERVQPRTNAICTMMEREARAQLARPATGPFTGVPFLIKDCAQDY
AGLPTAYGSKALSGRVATEHASVVRRYLAAGLVIFGKTNLPELALKGVSDSRAFGRVNNPWNAAHTPGGSSGGAAAAVAS
GVVPMAAGNDGGGSIRIPAACCGLFGLKPSRGLVSSGPGFGEYWFGASCEGVLSRSVRDTAAALDVISGAEPGDPFLVAE
PGVVFANAVTRDPPRLRIGVTTASPIGTEVHAEAKLAVETTVKLLRGLGHEVEEAAPEIDGAALATSFLHIYFGQVAAFV
AEARANGARGDQFELLTRVLATLGSAISAGALTTQLLKWNEFARALARFHARYDLLLTPTLAHPPIRHGQGDPSTAEQTL
LDMLDRIGLLGLLTRWGALDGMIDKIARDSLQYVPFTQLANLTGTPAMSVPLHWTADGLPLGVQFTGRLGDEARLLQLAR
QLEQAQPWFDRLPDWVRSD
>Mature_499_residues
MKLQDFLQRDATALAEAVRAGETTAAELLQLAMQQSERVQPRTNAICTMMEREARAQLARPATGPFTGVPFLIKDCAQDY
AGLPTAYGSKALSGRVATEHASVVRRYLAAGLVIFGKTNLPELALKGVSDSRAFGRVNNPWNAAHTPGGSSGGAAAAVAS
GVVPMAAGNDGGGSIRIPAACCGLFGLKPSRGLVSSGPGFGEYWFGASCEGVLSRSVRDTAAALDVISGAEPGDPFLVAE
PGVVFANAVTRDPPRLRIGVTTASPIGTEVHAEAKLAVETTVKLLRGLGHEVEEAAPEIDGAALATSFLHIYFGQVAAFV
AEARANGARGDQFELLTRVLATLGSAISAGALTTQLLKWNEFARALARFHARYDLLLTPTLAHPPIRHGQGDPSTAEQTL
LDMLDRIGLLGLLTRWGALDGMIDKIARDSLQYVPFTQLANLTGTPAMSVPLHWTADGLPLGVQFTGRLGDEARLLQLAR
QLEQAQPWFDRLPDWVRSD

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=522, Percent_Identity=27.2030651340996, Blast_Score=119, Evalue=8e-27,
Organism=Homo sapiens, GI195972892, Length=237, Percent_Identity=31.2236286919831, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17556264, Length=312, Percent_Identity=30.1282051282051, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17538252, Length=355, Percent_Identity=27.6056338028169, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=456, Percent_Identity=27.6315789473684, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=297, Percent_Identity=29.2929292929293, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17537465, Length=200, Percent_Identity=30, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=359, Percent_Identity=27.0194986072423, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=359, Percent_Identity=27.0194986072423, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=293, Percent_Identity=24.2320819112628, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319685, Length=249, Percent_Identity=29.3172690763052, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320448, Length=189, Percent_Identity=30.1587301587302, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648113, Length=485, Percent_Identity=28.8659793814433, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21356731, Length=512, Percent_Identity=24.609375, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24644968, Length=246, Percent_Identity=29.2682926829268, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI161078093, Length=451, Percent_Identity=26.8292682926829, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI45550774, Length=259, Percent_Identity=27.4131274131274, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24648435, Length=206, Percent_Identity=29.6116504854369, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648437, Length=206, Percent_Identity=29.6116504854369, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24652985, Length=251, Percent_Identity=27.8884462151394, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19922090, Length=251, Percent_Identity=27.8884462151394, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24652981, Length=251, Percent_Identity=27.8884462151394, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24652983, Length=251, Percent_Identity=27.8884462151394, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648441, Length=131, Percent_Identity=35.1145038167939, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648439, Length=131, Percent_Identity=35.1145038167939, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 52732; Mature: 52732

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLQDFLQRDATALAEAVRAGETTAAELLQLAMQQSERVQPRTNAICTMMEREARAQLAR
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
PATGPFTGVPFLIKDCAQDYAGLPTAYGSKALSGRVATEHASVVRRYLAAGLVIFGKTNL
CCCCCCCCCHHHHHHHHHHHCCCCCHHCCHHHCCCHHHHHHHHHHHHHHCCEEEECCCCC
PELALKGVSDSRAFGRVNNPWNAAHTPGGSSGGAAAAVASGVVPMAAGNDGGGSIRIPAA
HHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEECCCCCCCEEEHHHH
CCGLFGLKPSRGLVSSGPGFGEYWFGASCEGVLSRSVRDTAAALDVISGAEPGDPFLVAE
HHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEC
PGVVFANAVTRDPPRLRIGVTTASPIGTEVHAEAKLAVETTVKLLRGLGHEVEEAAPEID
CCEEEEHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
GAALATSFLHIYFGQVAAFVAEARANGARGDQFELLTRVLATLGSAISAGALTTQLLKWN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFARALARFHARYDLLLTPTLAHPPIRHGQGDPSTAEQTLLDMLDRIGLLGLLTRWGALD
HHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GMIDKIARDSLQYVPFTQLANLTGTPAMSVPLHWTADGLPLGVQFTGRLGDEARLLQLAR
HHHHHHHHHHHCCCCHHHHHCCCCCCCEECCEEECCCCCEEEEEECCCCCCHHHHHHHHH
QLEQAQPWFDRLPDWVRSD
HHHHCCCHHHHCCHHHCCC
>Mature Secondary Structure
MKLQDFLQRDATALAEAVRAGETTAAELLQLAMQQSERVQPRTNAICTMMEREARAQLAR
CCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
PATGPFTGVPFLIKDCAQDYAGLPTAYGSKALSGRVATEHASVVRRYLAAGLVIFGKTNL
CCCCCCCCCHHHHHHHHHHHCCCCCHHCCHHHCCCHHHHHHHHHHHHHHCCEEEECCCCC
PELALKGVSDSRAFGRVNNPWNAAHTPGGSSGGAAAAVASGVVPMAAGNDGGGSIRIPAA
HHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEECCCCCCCEEEHHHH
CCGLFGLKPSRGLVSSGPGFGEYWFGASCEGVLSRSVRDTAAALDVISGAEPGDPFLVAE
HHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEC
PGVVFANAVTRDPPRLRIGVTTASPIGTEVHAEAKLAVETTVKLLRGLGHEVEEAAPEID
CCEEEEHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCC
GAALATSFLHIYFGQVAAFVAEARANGARGDQFELLTRVLATLGSAISAGALTTQLLKWN
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFARALARFHARYDLLLTPTLAHPPIRHGQGDPSTAEQTLLDMLDRIGLLGLLTRWGALD
HHHHHHHHHHHHHCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
GMIDKIARDSLQYVPFTQLANLTGTPAMSVPLHWTADGLPLGVQFTGRLGDEARLLQLAR
HHHHHHHHHHHCCCCHHHHHCCCCCCCEECCEEECCCCCEEEEEECCCCCCHHHHHHHHH
QLEQAQPWFDRLPDWVRSD
HHHHCCCHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]