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The map label for this gene is glcD [H]

Identifier: 146342450

GI number: 146342450

Start: 5797072

End: 5798493

Strand: Reverse

Name: glcD [H]

Synonym: BRADO5609

Alternate gene names: 146342450

Gene position: 5798493-5797072 (Counterclockwise)

Preceding gene: 146342452

Following gene: 146342445

Centisome position: 77.76

GC content: 67.79

Gene sequence:

>1422_bases
GTGGCGACGACGATCCCCCATCACGATCTGAAGCGGCCCGCGCCGGAGGCGCTGAAAAGCGCGCTCGACGCGCTCGCGGC
CCGCTTCGGCAACAGGCTCATCACCTCGCAGGCCGTGCGCGAGCAGCATGGCCATACCACGACCTGGCTGCCGACGCAGC
CGCCGGATGGTGTGGTCCTGGCGCAGGAGACCGCCGACATCCAGGACGTCGTCCGCATCTGCGCCCCGCTCGGCGTGCCG
ATCATTCCGTTCGGCACCGGCACCTCGCTCGAGGGCCAGGTCAACGCGCCCGCCGGCGGCATCTGCATCGACATGCGCGA
CATGAACAAGGTGCTGGAGGTGCATGCCGAGGATCTCGACTGCGTGATCCAGCCCGGCATCACCCGCAAGACGCTGAACG
AATATCTCCGCGACCAGGGCCTGTTCTTCCCGATCGATCCCGGAGCCGACGCCTCGCTCGGCGGCATGGCCTCGACGCGC
GCCTCCGGTACCAATGCGGTGCGCTACGGCACCATCCGCGAGAACGTGCTGGCGCTGAAGGTGGTGCGCGGCGACGGCGA
GATCATCAAGACCGGCACCCGCGCCAAGAAGTCGGCCGCCGGCTACGACCTGACGCATCTGTTCATCGGCGCCGAAGGCA
CGCTCGGTATCATCTGCGAGATGACGATCCGCCTGCGCGGCATTCCCGAGACGATCGCGGCCGCCTCGTGCTCGTTCGAG
ACCGTGCGCGGCGCCTGCCAGGCGACGATCCTCGCGATCCAGACCGGCATCCCGATGGCGCGGATCGAACTCCTCAACGC
GGCGCAGGTGCGGGCGTGCAACGCCTATTCGAAGCTGACCCTGCCGGAGACGCCGCTGCTGCTCTTGGAGTTCCACGGCA
GCGAAACCGAGGTCGCCGAGCAGTCGCGGAATTTCGCCGAGATCGCCAGGGAATGCGGCGGCGGCGACTTCGCCTGGACC
ACCAAGCCGGAGGACCGCACCAGGCTGTGGCAGGCGCGTCATGATGCCTATTGGTCCGTCAAGGCGATCCGGCCCGGCGT
CGGTGTCGTCGCGACCGACGTCTGCGTGCCGATCTCGCGTCTCGCCGATTGCGTCGGCGAGACCGAGGAGGACCTCGCGC
GTCTCAAGCTGACCTCGCCGATCGTCGGTCATGTCGGCGACGGCAATTTCCACTGCTCGCTGATGTGCGACGTCGACGAT
GCCGACGAGATGGCGCGCGGCGAGGACTTCATGCATCGCCTCGTGGAGCGCGCCCAGGCGATGGGCGGCACCTGCACCGG
CGAGCACGGCATCGGCCAGGGCAAGCAGAAGTACCTGAAGTCGGAGCTCGGCCAGGAAGCGCTCGACGCCATGCGCGCCG
TCAAGCAGGCGCTCGACCCGCAGAACATCTTCAACCCTGGCAAGATCATTCCGGCGAATTGA

Upstream 100 bases:

>100_bases
CCGGCGTAGGCACTGCATTATCGCTCATCGGCTCTTCCGTTTGCTCCCTTGACCCTTGTTGCGTCCTTTGCCACGGTCCG
CCCAACTTTCGGAGGAAGCT

Downstream 100 bases:

>100_bases
TCGCCTTCATTGAAGCCGAGCTGTTGCCGCCGGCATTGCGGCGACAGCTCCGAACTCACCTCATCAGAAAAGCATCAAAT
GAAAACGCCGCCGCATCCGG

Product: putative D-lactate dehydrogenase

Products: pyruvate; ferrocytochrome c

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MATTIPHHDLKRPAPEALKSALDALAARFGNRLITSQAVREQHGHTTTWLPTQPPDGVVLAQETADIQDVVRICAPLGVP
IIPFGTGTSLEGQVNAPAGGICIDMRDMNKVLEVHAEDLDCVIQPGITRKTLNEYLRDQGLFFPIDPGADASLGGMASTR
ASGTNAVRYGTIRENVLALKVVRGDGEIIKTGTRAKKSAAGYDLTHLFIGAEGTLGIICEMTIRLRGIPETIAAASCSFE
TVRGACQATILAIQTGIPMARIELLNAAQVRACNAYSKLTLPETPLLLLEFHGSETEVAEQSRNFAEIARECGGGDFAWT
TKPEDRTRLWQARHDAYWSVKAIRPGVGVVATDVCVPISRLADCVGETEEDLARLKLTSPIVGHVGDGNFHCSLMCDVDD
ADEMARGEDFMHRLVERAQAMGGTCTGEHGIGQGKQKYLKSELGQEALDAMRAVKQALDPQNIFNPGKIIPAN

Sequences:

>Translated_473_residues
MATTIPHHDLKRPAPEALKSALDALAARFGNRLITSQAVREQHGHTTTWLPTQPPDGVVLAQETADIQDVVRICAPLGVP
IIPFGTGTSLEGQVNAPAGGICIDMRDMNKVLEVHAEDLDCVIQPGITRKTLNEYLRDQGLFFPIDPGADASLGGMASTR
ASGTNAVRYGTIRENVLALKVVRGDGEIIKTGTRAKKSAAGYDLTHLFIGAEGTLGIICEMTIRLRGIPETIAAASCSFE
TVRGACQATILAIQTGIPMARIELLNAAQVRACNAYSKLTLPETPLLLLEFHGSETEVAEQSRNFAEIARECGGGDFAWT
TKPEDRTRLWQARHDAYWSVKAIRPGVGVVATDVCVPISRLADCVGETEEDLARLKLTSPIVGHVGDGNFHCSLMCDVDD
ADEMARGEDFMHRLVERAQAMGGTCTGEHGIGQGKQKYLKSELGQEALDAMRAVKQALDPQNIFNPGKIIPAN
>Mature_472_residues
ATTIPHHDLKRPAPEALKSALDALAARFGNRLITSQAVREQHGHTTTWLPTQPPDGVVLAQETADIQDVVRICAPLGVPI
IPFGTGTSLEGQVNAPAGGICIDMRDMNKVLEVHAEDLDCVIQPGITRKTLNEYLRDQGLFFPIDPGADASLGGMASTRA
SGTNAVRYGTIRENVLALKVVRGDGEIIKTGTRAKKSAAGYDLTHLFIGAEGTLGIICEMTIRLRGIPETIAAASCSFET
VRGACQATILAIQTGIPMARIELLNAAQVRACNAYSKLTLPETPLLLLEFHGSETEVAEQSRNFAEIARECGGGDFAWTT
KPEDRTRLWQARHDAYWSVKAIRPGVGVVATDVCVPISRLADCVGETEEDLARLKLTSPIVGHVGDGNFHCSLMCDVDDA
DEMARGEDFMHRLVERAQAMGGTCTGEHGIGQGKQKYLKSELGQEALDAMRAVKQALDPQNIFNPGKIIPAN

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=465, Percent_Identity=49.8924731182796, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI37595754, Length=488, Percent_Identity=47.5409836065574, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI119964728, Length=429, Percent_Identity=28.2051282051282, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI4501993, Length=275, Percent_Identity=29.8181818181818, Blast_Score=99, Evalue=8e-21,
Organism=Escherichia coli, GI1789351, Length=422, Percent_Identity=29.1469194312796, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1787977, Length=493, Percent_Identity=25.3549695740365, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI48994907, Length=208, Percent_Identity=25.4807692307692, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI48994914, Length=157, Percent_Identity=26.1146496815287, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71992373, Length=460, Percent_Identity=45.4347826086956, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI71992381, Length=460, Percent_Identity=43.9130434782609, Blast_Score=396, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71992392, Length=377, Percent_Identity=49.3368700265252, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17534361, Length=446, Percent_Identity=26.6816143497758, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17556096, Length=465, Percent_Identity=24.0860215053763, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320027, Length=486, Percent_Identity=34.9794238683128, Blast_Score=290, Evalue=4e-79,
Organism=Saccharomyces cerevisiae, GI6320023, Length=425, Percent_Identity=27.7647058823529, Blast_Score=142, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6320764, Length=473, Percent_Identity=25.7928118393235, Blast_Score=137, Evalue=4e-33,
Organism=Drosophila melanogaster, GI18921117, Length=423, Percent_Identity=26.4775413711584, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24639277, Length=423, Percent_Identity=26.4775413711584, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24639275, Length=423, Percent_Identity=26.4775413711584, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24653753, Length=457, Percent_Identity=24.72647702407, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.2.4

Molecular weight: Translated: 50837; Mature: 50705

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTIPHHDLKRPAPEALKSALDALAARFGNRLITSQAVREQHGHTTTWLPTQPPDGVVL
CCCCCCCHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEECCCCCCCCEEE
AQETADIQDVVRICAPLGVPIIPFGTGTSLEGQVNAPAGGICIDMRDMNKVLEVHAEDLD
ECCCCCHHHHHHHHHHCCCCEEECCCCCCCCCEECCCCCCEEEEHHHHHHHHHHHHCCCC
CVIQPGITRKTLNEYLRDQGLFFPIDPGADASLGGMASTRASGTNAVRYGTIRENVLALK
EEECCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCEEECCHHCCEEEEE
VVRGDGEIIKTGTRAKKSAAGYDLTHLFIGAEGTLGIICEMTIRLRGIPETIAAASCSFE
EEECCCCEEECCCCHHHHCCCCCEEEEEEECCCCEEEEEEEEEEECCCCHHHHHHCCCHH
TVRGACQATILAIQTGIPMARIELLNAAQVRACNAYSKLTLPETPLLLLEFHGSETEVAE
HHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCEEEEEECCCCHHHHH
QSRNFAEIARECGGGDFAWTTKPEDRTRLWQARHDAYWSVKAIRPGVGVVATDVCVPISR
HCCCHHHHHHHCCCCCCEECCCCCHHHHHHHHHCCCEEEEEEECCCCCEEHHHHHHHHHH
LADCVGETEEDLARLKLTSPIVGHVGDGNFHCSLMCDVDDADEMARGEDFMHRLVERAQA
HHHHHCCCHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCHHHHHCCHHHHHHHHHHHHH
MGGTCTGEHGIGQGKQKYLKSELGQEALDAMRAVKQALDPQNIFNPGKIIPAN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCC
>Mature Secondary Structure 
ATTIPHHDLKRPAPEALKSALDALAARFGNRLITSQAVREQHGHTTTWLPTQPPDGVVL
CCCCCCHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEEECCCCCCCCEEE
AQETADIQDVVRICAPLGVPIIPFGTGTSLEGQVNAPAGGICIDMRDMNKVLEVHAEDLD
ECCCCCHHHHHHHHHHCCCCEEECCCCCCCCCEECCCCCCEEEEHHHHHHHHHHHHCCCC
CVIQPGITRKTLNEYLRDQGLFFPIDPGADASLGGMASTRASGTNAVRYGTIRENVLALK
EEECCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCEEECCHHCCEEEEE
VVRGDGEIIKTGTRAKKSAAGYDLTHLFIGAEGTLGIICEMTIRLRGIPETIAAASCSFE
EEECCCCEEECCCCHHHHCCCCCEEEEEEECCCCEEEEEEEEEEECCCCHHHHHHCCCHH
TVRGACQATILAIQTGIPMARIELLNAAQVRACNAYSKLTLPETPLLLLEFHGSETEVAE
HHHHHHHHEEEEEECCCCHHHHHHHHHHHHHHHCCHHCCCCCCCCEEEEEECCCCHHHHH
QSRNFAEIARECGGGDFAWTTKPEDRTRLWQARHDAYWSVKAIRPGVGVVATDVCVPISR
HCCCHHHHHHHCCCCCCEECCCCCHHHHHHHHHCCCEEEEEEECCCCCEEHHHHHHHHHH
LADCVGETEEDLARLKLTSPIVGHVGDGNFHCSLMCDVDDADEMARGEDFMHRLVERAQA
HHHHHCCCHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCHHHHHCCHHHHHHHHHHHHH
MGGTCTGEHGIGQGKQKYLKSELGQEALDAMRAVKQALDPQNIFNPGKIIPAN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; ferricytochrome c

Specific reaction: (R)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]