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The map label for this gene is yafC [H]

Identifier: 146342446

GI number: 146342446

Start: 5793140

End: 5794057

Strand: Direct

Name: yafC [H]

Synonym: BRADO5605

Alternate gene names: 146342446

Gene position: 5793140-5794057 (Clockwise)

Preceding gene: 146342431

Following gene: 146342447

Centisome position: 77.69

GC content: 69.61

Gene sequence:

>918_bases
TTGCTCGATCGCCTGACCGGGCTGGAAGTGCTGTCCAAGGTGGCCGGCAGCGGCAGCCTGTCGGGCGCCGCGCGCCTGCT
CGGGATGTCGCCGACCATGGTGACGAAGCATCTCGCGGCGCTGGAAACGCGGCTCGGCACCAAGCTGTTTCACCGCACCA
CCCGCCGGCTGACGATCACCGAGGCAGGGCGGCGCTATCTCGATCTCGCCGAGCGGGTGCTCGGCGATTTCGAGGCCGCC
GAGAGCGCGATTGCGGCCGATCGCGTCGAGCCGCGCGGCGTGCTGCGCCTCAACGTCCCGCTCGCCTTCGGCACCCGGCA
GATTGCGCCGCTGCTTGCCGGCTTCGCCGCGCTCTATCCTGGCGTCAGCGTCGAGCTCGGCCTCAACGACCGGCTGGTCG
ACCTGATCGAGGAAGGCTGGGATCTGGCGATCCGGATCGGCACGCTGAAAGACTCCACCTTGGTGGCGCGTCGGCTGGCG
CCGTGCCGGATGATGATCTGTGCCGCGCCATCCTATCTCACCACGCATGGCACACCGCGCACGACAACCGACCTCGCCGC
GCACAATTGCCTCGGCTATACGCTCGCCGCCGCCGGCCCGTCGCGCTGGCGCATGGGCGGCAAGGCGGCGATCGACGTGA
CCGTCAGCGGCAACCTGCGCGCCAACAACGGCGATGCGCTGCTCGCTGCGGCGCTCGCCGGCCAGGGCCTGATCTATCAG
CCGACCTTCATCGTCGGCGACGAGTTGCGGACCGGACGGCTGGTGCCGGTGACGCTCGACGTTCCGCCATCCGATCTGCT
GGCGGTGCACGCGGTGTACCTGCCGGACCGCACGCCCTCGGCCAAGGTACGCGCCTTCATCGATTACCTCGCCGCGGCAT
TCGCGCCGCTCCCCCCATGGGATCGCGACCTTGGATAA

Upstream 100 bases:

>100_bases
CAGAACCTCCGTGGGACACCGGGGATATGGATCATTCCCTGCCGAGCGATAATCCGGTACAAGGGAAATTCAGTTCACAC
TGAGAGAGCGAGATCCAGGG

Downstream 100 bases:

>100_bases
GCACACGCGCTGCCTGCGCGCGGAGACATCGACATGACTGAGCAACCTCACGACGGCAGCCATCATTGGGCGATGCTGCG
CTCGCTGCAGGGCTGGCGGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MLDRLTGLEVLSKVAGSGSLSGAARLLGMSPTMVTKHLAALETRLGTKLFHRTTRRLTITEAGRRYLDLAERVLGDFEAA
ESAIAADRVEPRGVLRLNVPLAFGTRQIAPLLAGFAALYPGVSVELGLNDRLVDLIEEGWDLAIRIGTLKDSTLVARRLA
PCRMMICAAPSYLTTHGTPRTTTDLAAHNCLGYTLAAAGPSRWRMGGKAAIDVTVSGNLRANNGDALLAAALAGQGLIYQ
PTFIVGDELRTGRLVPVTLDVPPSDLLAVHAVYLPDRTPSAKVRAFIDYLAAAFAPLPPWDRDLG

Sequences:

>Translated_305_residues
MLDRLTGLEVLSKVAGSGSLSGAARLLGMSPTMVTKHLAALETRLGTKLFHRTTRRLTITEAGRRYLDLAERVLGDFEAA
ESAIAADRVEPRGVLRLNVPLAFGTRQIAPLLAGFAALYPGVSVELGLNDRLVDLIEEGWDLAIRIGTLKDSTLVARRLA
PCRMMICAAPSYLTTHGTPRTTTDLAAHNCLGYTLAAAGPSRWRMGGKAAIDVTVSGNLRANNGDALLAAALAGQGLIYQ
PTFIVGDELRTGRLVPVTLDVPPSDLLAVHAVYLPDRTPSAKVRAFIDYLAAAFAPLPPWDRDLG
>Mature_305_residues
MLDRLTGLEVLSKVAGSGSLSGAARLLGMSPTMVTKHLAALETRLGTKLFHRTTRRLTITEAGRRYLDLAERVLGDFEAA
ESAIAADRVEPRGVLRLNVPLAFGTRQIAPLLAGFAALYPGVSVELGLNDRLVDLIEEGWDLAIRIGTLKDSTLVARRLA
PCRMMICAAPSYLTTHGTPRTTTDLAAHNCLGYTLAAAGPSRWRMGGKAAIDVTVSGNLRANNGDALLAAALAGQGLIYQ
PTFIVGDELRTGRLVPVTLDVPPSDLLAVHAVYLPDRTPSAKVRAFIDYLAAAFAPLPPWDRDLG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=35.958904109589, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI87081978, Length=297, Percent_Identity=36.026936026936, Blast_Score=164, Evalue=6e-42,
Organism=Escherichia coli, GI1789440, Length=286, Percent_Identity=32.5174825174825, Blast_Score=158, Evalue=4e-40,
Organism=Escherichia coli, GI145693193, Length=301, Percent_Identity=32.5581395348837, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=32.7645051194539, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1787128, Length=284, Percent_Identity=31.6901408450704, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI1787589, Length=281, Percent_Identity=28.8256227758007, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790011, Length=258, Percent_Identity=26.7441860465116, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1789173, Length=297, Percent_Identity=29.2929292929293, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1787806, Length=137, Percent_Identity=33.5766423357664, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788706, Length=292, Percent_Identity=23.972602739726, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788481, Length=303, Percent_Identity=25.0825082508251, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI145693105, Length=159, Percent_Identity=28.9308176100629, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786448, Length=241, Percent_Identity=24.0663900414938, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI157672245, Length=113, Percent_Identity=35.3982300884956, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32528; Mature: 32528

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDRLTGLEVLSKVAGSGSLSGAARLLGMSPTMVTKHLAALETRLGTKLFHRTTRRLTIT
CCCCHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEH
EAGRRYLDLAERVLGDFEAAESAIAADRVEPRGVLRLNVPLAFGTRQIAPLLAGFAALYP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEECCHHHHHHHHHHHHHHCC
GVSVELGLNDRLVDLIEEGWDLAIRIGTLKDSTLVARRLAPCRMMICAAPSYLTTHGTPR
CCEEEECCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCHHHEEEEECCCCEECCCCCC
TTTDLAAHNCLGYTLAAAGPSRWRMGGKAAIDVTVSGNLRANNGDALLAAALAGQGLIYQ
CHHHHHHHHHHCEEEECCCCHHHCCCCCEEEEEEEECCEEECCCCEEEEEHHHCCCEEEC
PTFIVGDELRTGRLVPVTLDVPPSDLLAVHAVYLPDRTPSAKVRAFIDYLAAAFAPLPPW
CEEEECCCCCCCEEEEEEECCCCCHHEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
DRDLG
CCCCC
>Mature Secondary Structure
MLDRLTGLEVLSKVAGSGSLSGAARLLGMSPTMVTKHLAALETRLGTKLFHRTTRRLTIT
CCCCHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEH
EAGRRYLDLAERVLGDFEAAESAIAADRVEPRGVLRLNVPLAFGTRQIAPLLAGFAALYP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCEECCHHHHHHHHHHHHHHCC
GVSVELGLNDRLVDLIEEGWDLAIRIGTLKDSTLVARRLAPCRMMICAAPSYLTTHGTPR
CCEEEECCCHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCHHHEEEEECCCCEECCCCCC
TTTDLAAHNCLGYTLAAAGPSRWRMGGKAAIDVTVSGNLRANNGDALLAAALAGQGLIYQ
CHHHHHHHHHHCEEEECCCCHHHCCCCCEEEEEEEECCEEECCCCEEEEEHHHCCCEEEC
PTFIVGDELRTGRLVPVTLDVPPSDLLAVHAVYLPDRTPSAKVRAFIDYLAAAFAPLPPW
CEEEECCCCCCCEEEEEEECCCCCHHEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCC
DRDLG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]