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The map label for this gene is miaA

Identifier: 146342408

GI number: 146342408

Start: 5752063

End: 5752989

Strand: Reverse

Name: miaA

Synonym: BRADO5567

Alternate gene names: 146342408

Gene position: 5752989-5752063 (Counterclockwise)

Preceding gene: 146342413

Following gene: 146342407

Centisome position: 77.15

GC content: 68.18

Gene sequence:

>927_bases
GTGACTTTGCTAAGCGACAACAAAGCGGTGCTTATCGCAGGGCCGACCGCCAGCGGCAAGTCGGCGCTGGCGTTGGAACT
TGCGCAAAAAGCGGGCGCGGTCATCATCAACACCGACTCGATGCAAGTCTACCACGATCTGCGGGTGCTGACGGCGCGGC
CGGCACCCGAGGACGAGGCGCTGGTCCCGCATCGGCTTTACGGTCACGTCGATGCTGCGGTGAACTACTCGGCCGGCCAT
TATGTCCGTGATGCCGCTCAGGTTCTCGACGAGGTCAGGCGCGAGGGCCGGCTGCCGGTCTTCATTGGCGGCACCGGCCT
GTACTTCAAGGCGCTGACACGCGGATTGTCGGCGGTGCCGCCGGTGCCGGACGACATTCGCGAGGCGGTGCGTCTCAAGC
TCGATCGCGACGGTGTCGAGGCGCTGCACGCCGAACTGGCGCGGCGCGATGCCGCGGCGGCGGCGCGGCTGAACGTCCGC
GACCGCACCCGCATCGCCCGGGCGCTGGAGGTCATCGAAGCGACCGGTCGGCCATTGGCCGACTGGCACGCCGAGACCAC
GCCGCCGTTGCTGCCCCCGGAGCGCACGCATGCGCTGTTCATCGCGCCGGAGCGCGAGGCGCTGTATGCCAGGATCGACG
CGCGCTTCGAGACGATGCTCGAGGCGGGCGCGCTCGCGGAGGTCGAGCGGCTCGCCGCCCGCGGCCTCGATCCGCTGCTG
CCGGCCATGAAGGCCCACGGCGTGCCGGCGCTGATCCGTTACCTCCGGGGCGAGATCACCCGCGAGGAGGCGGCCACCAT
CGGCAAGGCCGATACAAGGCATTATGCCAAGCGGCAGTTCACCTGGTTTCGGCATCAGCTGCCGGAGTTCGAGTGGATGA
GCCCGGAGGCAGCAAAAGGGTTGGTCAGAGCGTTGAAGGTTCGATGA

Upstream 100 bases:

>100_bases
TGCCCGGCGATGGCAGCACGGCCAGGAAGGCCTCCACGATCGTGGTATCAAGTGCGGGATCGGCGGGATTGCAGATGAAG
GTGGCGACGAGAGACATCGG

Downstream 100 bases:

>100_bases
TATCGTGATATCAAGTCATCGGAGGCGATGATGGCCGATATCCTGATCCGCAAAGTAGACGACGAGACCAAGGAGTTGCT
TCGGCTCAGAGCCGAACGCC

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTLLSDNKAVLIAGPTASGKSALALELAQKAGAVIINTDSMQVYHDLRVLTARPAPEDEALVPHRLYGHVDAAVNYSAGH
YVRDAAQVLDEVRREGRLPVFIGGTGLYFKALTRGLSAVPPVPDDIREAVRLKLDRDGVEALHAELARRDAAAAARLNVR
DRTRIARALEVIEATGRPLADWHAETTPPLLPPERTHALFIAPEREALYARIDARFETMLEAGALAEVERLAARGLDPLL
PAMKAHGVPALIRYLRGEITREEAATIGKADTRHYAKRQFTWFRHQLPEFEWMSPEAAKGLVRALKVR

Sequences:

>Translated_308_residues
MTLLSDNKAVLIAGPTASGKSALALELAQKAGAVIINTDSMQVYHDLRVLTARPAPEDEALVPHRLYGHVDAAVNYSAGH
YVRDAAQVLDEVRREGRLPVFIGGTGLYFKALTRGLSAVPPVPDDIREAVRLKLDRDGVEALHAELARRDAAAAARLNVR
DRTRIARALEVIEATGRPLADWHAETTPPLLPPERTHALFIAPEREALYARIDARFETMLEAGALAEVERLAARGLDPLL
PAMKAHGVPALIRYLRGEITREEAATIGKADTRHYAKRQFTWFRHQLPEFEWMSPEAAKGLVRALKVR
>Mature_307_residues
TLLSDNKAVLIAGPTASGKSALALELAQKAGAVIINTDSMQVYHDLRVLTARPAPEDEALVPHRLYGHVDAAVNYSAGHY
VRDAAQVLDEVRREGRLPVFIGGTGLYFKALTRGLSAVPPVPDDIREAVRLKLDRDGVEALHAELARRDAAAAARLNVRD
RTRIARALEVIEATGRPLADWHAETTPPLLPPERTHALFIAPEREALYARIDARFETMLEAGALAEVERLAARGLDPLLP
AMKAHGVPALIRYLRGEITREEAATIGKADTRHYAKRQFTWFRHQLPEFEWMSPEAAKGLVRALKVR

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=308, Percent_Identity=26.2987012987013, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1790613, Length=279, Percent_Identity=40.1433691756272, Blast_Score=183, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI25144712, Length=237, Percent_Identity=24.0506329113924, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324848, Length=295, Percent_Identity=28.8135593220339, Blast_Score=133, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24649830, Length=236, Percent_Identity=29.6610169491525, Blast_Score=98, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_BRASO (A4YZB3)

Other databases:

- EMBL:   CU234118
- RefSeq:   YP_001207456.1
- ProteinModelPortal:   A4YZB3
- STRING:   A4YZB3
- GeneID:   5119452
- GenomeReviews:   CU234118_GR
- KEGG:   bra:BRADO5567
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   BSP376:BRADO5567-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 33950; Mature: 33819

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLSDNKAVLIAGPTASGKSALALELAQKAGAVIINTDSMQVYHDLRVLTARPAPEDEA
CCEECCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHHHHEECCCCCCCCC
LVPHRLYGHVDAAVNYSAGHYVRDAAQVLDEVRREGRLPVFIGGTGLYFKALTRGLSAVP
CCCHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCCCC
PVPDDIREAVRLKLDRDGVEALHAELARRDAAAAARLNVRDRTRIARALEVIEATGRPLA
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCH
DWHAETTPPLLPPERTHALFIAPEREALYARIDARFETMLEAGALAEVERLAARGLDPLL
HCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
PAMKAHGVPALIRYLRGEITREEAATIGKADTRHYAKRQFTWFRHQLPEFEWMSPEAAKG
HHHHHCCHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
LVRALKVR
HHHHHHCC
>Mature Secondary Structure 
TLLSDNKAVLIAGPTASGKSALALELAQKAGAVIINTDSMQVYHDLRVLTARPAPEDEA
CEECCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHHHHEECCCCCCCCC
LVPHRLYGHVDAAVNYSAGHYVRDAAQVLDEVRREGRLPVFIGGTGLYFKALTRGLSAVP
CCCHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCCCCCCC
PVPDDIREAVRLKLDRDGVEALHAELARRDAAAAARLNVRDRTRIARALEVIEATGRPLA
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCH
DWHAETTPPLLPPERTHALFIAPEREALYARIDARFETMLEAGALAEVERLAARGLDPLL
HCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
PAMKAHGVPALIRYLRGEITREEAATIGKADTRHYAKRQFTWFRHQLPEFEWMSPEAAKG
HHHHHCCHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
LVRALKVR
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA