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The map label for this gene is stcD [H]

Identifier: 146342359

GI number: 146342359

Start: 5697440

End: 5699443

Strand: Direct

Name: stcD [H]

Synonym: BRADO5515

Alternate gene names: 146342359

Gene position: 5697440-5699443 (Clockwise)

Preceding gene: 146342347

Following gene: 146342360

Centisome position: 76.41

GC content: 67.17

Gene sequence:

>2004_bases
TTGTCGCAGCATTGGCCATACGCACAGGTGCTGAGCCCGCTCGATGTCGGTCGCCTACGCTTCCGCAACCGCATCTTCGT
TCCCGCGCACACCACGAATTTCGGCGAGCACCACCTCCCGAGCGCGCAGCACCTCGCCTATCATCACGCTCGTGCTTGTG
GCGGCGTGGGCGCGATCATCTTCGAATCCATTCGTGTGCACGCCAATTCGCTGGGCCGTCCGCAAGCGGTGTGCGGCTTC
GATCCCGCCTGCATTGATCCCTTCCACCGCATCACCGATGCGGTGAAGGCCGAAGGCGCCTGCATGCTCGGCCAGATCAT
CCATCTGGGGCGGCAGGTCGAGGGCGATTTCGAGCGGACGGTGTCGTGGGGCGCCTCGCCCATTCCGTGGTCGATCTCGG
CGCTGCCGCCGCGGCCGATGGACGAGTTCGACATGGAGCAGGTGATCGAGGGGCACCTCGTCACCGCGCGCAACCTCGTC
GCGGCCGGATTCGACGGCATCGAGCTGCAGATGGCGCATGGTCATCTGCTGCAGCAGTTCATGTCACCTCTGTCGAACAA
GCGCGATGATGAATACGGCGGCACGCTGGAGAACCGTCTGCGCTTCCCGGCCCGTGTGCTCGCGGCGTTGCGGGCCGAGC
TTGGCGACGAATTCTGTCTCGGCATCCGCATCAGCGGCGAGGAATATGCCGACGGTGGCCTTGGCATCGACGAGGCCGCG
CGGATCGTGGCAATGCTGGCGCGGCAGACCCAGATCGACTTCGTCAACGTCTCGCATTCAGCCTACGTCGCGAACTATTC
GCTGGCGACGCAGATGGCCGACATGGCGCTCGATCCGTCGCCGTTCCGCGCTCTGCCCGCGCGCATCCGCCTCGAGCTGC
GCGCGCAGGGATTCGGGACGCCCGTGTTCGCCGTGTGCCGATTTACGGGACTTGACGAGGCCGAGGCGATGATCGCCGCG
GGCGCTGCCGACGCCATCGGGATGGCGCGCGCGCATCTCGCCGAACCGGCCATCGTGAAGAAGTCGGTCGAGGGACGTCT
CGACGAAATCCGCCGCTGCATCGGCTGCAACCAGGGCTGCGCCGGCATGCTGGAGCGGAACCTGCCGATCCGCTGCCTGA
TCAATCCGATTGCCGGACTGGAAGGGCAGTTCGACGAGCCCGAGGCGCTGCCTCGCACGGCGCCGCGACGCGTTCTCGTC
GTCGGCGGCGGGCCTGCAGGACTGGAAGCCGCGCGCGTGGCGGCTGCGCTCGGGCATCATGTCACGCTATGGGAGCGCAG
CGATCAGCTTGGCGGACAGTTGCGCACGGCGTGGCTGATGCCGAAGCGTACGAACTTCAAGGCGTTCGTGGAGTTCCAGA
TCGCGGCACTGAGGCGGCTCGGCGCGACCATCGTCTTCGACAAGGAGGCGGACGCCACAGGGATCTCCGCCTTCGGCGCC
GATCGCATCGTACTGGCGACGGGCTCCGCCACGACCGCTATGCCCATGGCCGGCAACGGCCCCGTGTTCACCCTGCCCGA
TGCGCTGCGCGCGCCGGGGCAGCTCGGCGCATCGGTCGCCGTGTTTGACCGCACCGGCGAATGGGGCACGCTGGCGGCGC
TGGAACATTTCGCCGATCTCGGCAAGGCCGTGACCTTGTTCGTGCCGGCGACGAGCTACGCCTGGCGCACCACGATCTAT
TCGACGCTCGCCAACAGCCGCCGCCTGCGCGAGCGCAAGGTCCGCATCGCGACCTTGCGCGCCGTTCGCGCATATGATGG
CGAGACGCTGGAGGTCGAGGATCTCTCGACCGGCGATGTCGAGCGCCTGACGGGGTTCTCGGCGCTGATCGCCGTCGATC
ACAACAGCACGGATCAGTCGCTGTATCTCGCCTTGCGGCGCGCCGGAAAGCCGGTGATGCAGGTCGGCGACAACAATGCG
CCGCGCACGGCGCTCGAAGCGGTCTATCAGGGTCACATGGCCGCGCGCGCCCCCTTCCCTGAAGCGGCCGAACATCCGAC
ATGA

Upstream 100 bases:

>100_bases
TAGGTGTATAGAAAATATAACAGCTTCGGTCAACCAGCTCGCGCGGCTCCTCTGGAGCAAGGATCGAGCCATGGATGGGG
CACCGGGAGGATCACGCGAT

Downstream 100 bases:

>100_bases
CTGGTGCGCTGATGATGCGATCGACTGAGGACATTCGGGAATGACGGGAACAGGGCAGCCGCGGACGCTGTTCGACAAGG
TCTGGGATGGGCATGTGGTG

Product: putative NADH-dependent flavin oxidoreductase N-methylproline demethylase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: Stachydrine utilization protein stcD [H]

Number of amino acids: Translated: 667; Mature: 666

Protein sequence:

>667_residues
MSQHWPYAQVLSPLDVGRLRFRNRIFVPAHTTNFGEHHLPSAQHLAYHHARACGGVGAIIFESIRVHANSLGRPQAVCGF
DPACIDPFHRITDAVKAEGACMLGQIIHLGRQVEGDFERTVSWGASPIPWSISALPPRPMDEFDMEQVIEGHLVTARNLV
AAGFDGIELQMAHGHLLQQFMSPLSNKRDDEYGGTLENRLRFPARVLAALRAELGDEFCLGIRISGEEYADGGLGIDEAA
RIVAMLARQTQIDFVNVSHSAYVANYSLATQMADMALDPSPFRALPARIRLELRAQGFGTPVFAVCRFTGLDEAEAMIAA
GAADAIGMARAHLAEPAIVKKSVEGRLDEIRRCIGCNQGCAGMLERNLPIRCLINPIAGLEGQFDEPEALPRTAPRRVLV
VGGGPAGLEAARVAAALGHHVTLWERSDQLGGQLRTAWLMPKRTNFKAFVEFQIAALRRLGATIVFDKEADATGISAFGA
DRIVLATGSATTAMPMAGNGPVFTLPDALRAPGQLGASVAVFDRTGEWGTLAALEHFADLGKAVTLFVPATSYAWRTTIY
STLANSRRLRERKVRIATLRAVRAYDGETLEVEDLSTGDVERLTGFSALIAVDHNSTDQSLYLALRRAGKPVMQVGDNNA
PRTALEAVYQGHMAARAPFPEAAEHPT

Sequences:

>Translated_667_residues
MSQHWPYAQVLSPLDVGRLRFRNRIFVPAHTTNFGEHHLPSAQHLAYHHARACGGVGAIIFESIRVHANSLGRPQAVCGF
DPACIDPFHRITDAVKAEGACMLGQIIHLGRQVEGDFERTVSWGASPIPWSISALPPRPMDEFDMEQVIEGHLVTARNLV
AAGFDGIELQMAHGHLLQQFMSPLSNKRDDEYGGTLENRLRFPARVLAALRAELGDEFCLGIRISGEEYADGGLGIDEAA
RIVAMLARQTQIDFVNVSHSAYVANYSLATQMADMALDPSPFRALPARIRLELRAQGFGTPVFAVCRFTGLDEAEAMIAA
GAADAIGMARAHLAEPAIVKKSVEGRLDEIRRCIGCNQGCAGMLERNLPIRCLINPIAGLEGQFDEPEALPRTAPRRVLV
VGGGPAGLEAARVAAALGHHVTLWERSDQLGGQLRTAWLMPKRTNFKAFVEFQIAALRRLGATIVFDKEADATGISAFGA
DRIVLATGSATTAMPMAGNGPVFTLPDALRAPGQLGASVAVFDRTGEWGTLAALEHFADLGKAVTLFVPATSYAWRTTIY
STLANSRRLRERKVRIATLRAVRAYDGETLEVEDLSTGDVERLTGFSALIAVDHNSTDQSLYLALRRAGKPVMQVGDNNA
PRTALEAVYQGHMAARAPFPEAAEHPT
>Mature_666_residues
SQHWPYAQVLSPLDVGRLRFRNRIFVPAHTTNFGEHHLPSAQHLAYHHARACGGVGAIIFESIRVHANSLGRPQAVCGFD
PACIDPFHRITDAVKAEGACMLGQIIHLGRQVEGDFERTVSWGASPIPWSISALPPRPMDEFDMEQVIEGHLVTARNLVA
AGFDGIELQMAHGHLLQQFMSPLSNKRDDEYGGTLENRLRFPARVLAALRAELGDEFCLGIRISGEEYADGGLGIDEAAR
IVAMLARQTQIDFVNVSHSAYVANYSLATQMADMALDPSPFRALPARIRLELRAQGFGTPVFAVCRFTGLDEAEAMIAAG
AADAIGMARAHLAEPAIVKKSVEGRLDEIRRCIGCNQGCAGMLERNLPIRCLINPIAGLEGQFDEPEALPRTAPRRVLVV
GGGPAGLEAARVAAALGHHVTLWERSDQLGGQLRTAWLMPKRTNFKAFVEFQIAALRRLGATIVFDKEADATGISAFGAD
RIVLATGSATTAMPMAGNGPVFTLPDALRAPGQLGASVAVFDRTGEWGTLAALEHFADLGKAVTLFVPATSYAWRTTIYS
TLANSRRLRERKVRIATLRAVRAYDGETLEVEDLSTGDVERLTGFSALIAVDHNSTDQSLYLALRRAGKPVMQVGDNNAP
RTALEAVYQGHMAARAPFPEAAEHPT

Specific function: Possible NADH-dependent oxidase, functions as a demethylase that converts N-methylproline to proline [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789463, Length=520, Percent_Identity=27.8846153846154, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1787939, Length=357, Percent_Identity=27.1708683473389, Blast_Score=99, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17564188, Length=383, Percent_Identity=28.9817232375979, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17559804, Length=373, Percent_Identity=28.4182305630027, Blast_Score=116, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17559802, Length=337, Percent_Identity=29.673590504451, Blast_Score=113, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI72001454, Length=360, Percent_Identity=28.6111111111111, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17540738, Length=384, Percent_Identity=26.5625, Blast_Score=105, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI17565138, Length=324, Percent_Identity=27.1604938271605, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17566914, Length=331, Percent_Identity=25.6797583081571, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6325086, Length=82, Percent_Identity=50, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321973, Length=90, Percent_Identity=41.1111111111111, Blast_Score=78, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 72177; Mature: 72046

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQHWPYAQVLSPLDVGRLRFRNRIFVPAHTTNFGEHHLPSAQHLAYHHARACGGVGAII
CCCCCCHHHHHCCCHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHH
FESIRVHANSLGRPQAVCGFDPACIDPFHRITDAVKAEGACMLGQIIHLGRQVEGDFERT
HHHHHHHHHCCCCCCEECCCCCHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHH
VSWGASPIPWSISALPPRPMDEFDMEQVIEGHLVTARNLVAAGFDGIELQMAHGHLLQQF
HHCCCCCCCEECCCCCCCCCCCCCHHHHHCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHH
MSPLSNKRDDEYGGTLENRLRFPARVLAALRAELGDEFCLGIRISGEEYADGGLGIDEAA
HHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHH
RIVAMLARQTQIDFVNVSHSAYVANYSLATQMADMALDPSPFRALPARIRLELRAQGFGT
HHHHHHHHHCCEEEEECCCCEEEEHHHHHHHHHHHCCCCCCHHHCCCCEEEEEEECCCCC
PVFAVCRFTGLDEAEAMIAAGAADAIGMARAHLAEPAIVKKSVEGRLDEIRRCIGCNQGC
HHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC
AGMLERNLPIRCLINPIAGLEGQFDEPEALPRTAPRRVLVVGGGPAGLEAARVAAALGHH
HHHHHCCCCEEEEECHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCE
VTLWERSDQLGGQLRTAWLMPKRTNFKAFVEFQIAALRRLGATIVFDKEADATGISAFGA
EEEEECCHHCCCCEEEEEECCCCCCCEEEHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
DRIVLATGSATTAMPMAGNGPVFTLPDALRAPGQLGASVAVFDRTGEWGTLAALEHFADL
CEEEEEECCCCEECCCCCCCCEEECCHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHC
GKAVTLFVPATSYAWRTTIYSTLANSRRLRERKVRIATLRAVRAYDGETLEVEDLSTGDV
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCH
ERLTGFSALIAVDHNSTDQSLYLALRRAGKPVMQVGDNNAPRTALEAVYQGHMAARAPFP
HHHCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCHHHCCCCC
EAAEHPT
CCCCCCC
>Mature Secondary Structure 
SQHWPYAQVLSPLDVGRLRFRNRIFVPAHTTNFGEHHLPSAQHLAYHHARACGGVGAII
CCCCCHHHHHCCCHHHHHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHCCHHHHH
FESIRVHANSLGRPQAVCGFDPACIDPFHRITDAVKAEGACMLGQIIHLGRQVEGDFERT
HHHHHHHHHCCCCCCEECCCCCHHCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHH
VSWGASPIPWSISALPPRPMDEFDMEQVIEGHLVTARNLVAAGFDGIELQMAHGHLLQQF
HHCCCCCCCEECCCCCCCCCCCCCHHHHHCCCHHHHHHHHHCCCCCEEEEEHHHHHHHHH
MSPLSNKRDDEYGGTLENRLRFPARVLAALRAELGDEFCLGIRISGEEYADGGLGIDEAA
HHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHHH
RIVAMLARQTQIDFVNVSHSAYVANYSLATQMADMALDPSPFRALPARIRLELRAQGFGT
HHHHHHHHHCCEEEEECCCCEEEEHHHHHHHHHHHCCCCCCHHHCCCCEEEEEEECCCCC
PVFAVCRFTGLDEAEAMIAAGAADAIGMARAHLAEPAIVKKSVEGRLDEIRRCIGCNQGC
HHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCC
AGMLERNLPIRCLINPIAGLEGQFDEPEALPRTAPRRVLVVGGGPAGLEAARVAAALGHH
HHHHHCCCCEEEEECHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCE
VTLWERSDQLGGQLRTAWLMPKRTNFKAFVEFQIAALRRLGATIVFDKEADATGISAFGA
EEEEECCHHCCCCEEEEEECCCCCCCEEEHHHHHHHHHHCCCEEEEECCCCCCCCCCCCC
DRIVLATGSATTAMPMAGNGPVFTLPDALRAPGQLGASVAVFDRTGEWGTLAALEHFADL
CEEEEEECCCCEECCCCCCCCEEECCHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHC
GKAVTLFVPATSYAWRTTIYSTLANSRRLRERKVRIATLRAVRAYDGETLEVEDLSTGDV
CCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCH
ERLTGFSALIAVDHNSTDQSLYLALRRAGKPVMQVGDNNAPRTALEAVYQGHMAARAPFP
HHHCCCEEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCHHHCCCCC
EAAEHPT
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9758825; 11481431 [H]